Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,755,150 A→T 100% L300* (TTG→TAG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,755,1500AT95.0% 563.0 / 15.1 180L300* (TTG→TAG) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base A (7/2);  new base T (89/82);  total (96/84)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 180 total aligned reads displayed.

CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294
                                                                                                                                                 |                                                                                                                                                
cTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTAc                                                                                                                                                >  1:1528600/1‑148 (MQ=255)
  gCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTg                                                                                                                                              >  2:3482622/1‑148 (MQ=255)
      tttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCgg                                                                                                                                          >  2:581274/1‑148 (MQ=255)
       ttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGc                                                                                                                                         <  2:622645/148‑1 (MQ=255)
        tCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCt                                                                                                                                        <  2:321517/148‑1 (MQ=255)
         cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                       <  2:250076/148‑1 (MQ=255)
         cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                       <  2:2022545/148‑1 (MQ=255)
           tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGtt                                                                                                                                     <  2:1330344/148‑1 (MQ=255)
             tCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTAc                                                                                                                                   >  1:646675/1‑148 (MQ=255)
                aCTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaa                                                                                                                                >  1:2925795/1‑148 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               <  1:3260413/148‑1 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               <  1:1989825/148‑1 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATATGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               >  2:350268/1‑148 (MQ=255)
                  ttCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaaa                                                                                                                              >  1:576354/1‑148 (MQ=255)
                       ccAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                         >  2:2862337/1‑148 (MQ=255)
                       ccAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                         <  2:2993058/148‑1 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        >  1:1368131/1‑148 (MQ=255)
                              gCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTgtgt                                                                                                                  <  2:2683367/148‑1 (MQ=255)
                              gCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTgtgt                                                                                                                  <  1:2780223/148‑1 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 <  2:2272801/148‑1 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 >  2:156721/1‑148 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 >  1:567689/1‑148 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:1801756/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:2321329/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  1:983322/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:949456/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                >  1:2174785/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               >  2:299546/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               >  2:299566/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               <  2:2448829/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            >  2:3218885/1‑148 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            >  1:3253359/1‑148 (MQ=255)
                                      gcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagcca                                                                                                          >  2:569966/1‑148 (MQ=255)
                                      gcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagcca                                                                                                          >  2:570433/1‑148 (MQ=255)
                                       cagATTGTTGATGGTCATCTGACCATCCGTTTTGAATGCGTGCTGCGCGACGTTGAACAAACCGTTAAATACCGCGCGCCCATTGTCGCTGACGATAGTTTTGAGCTACTGTCGGCTGTAACAAAAACCTTTATTTTGTTccagacag                                                                                                         >  2:877257/1‑148 (MQ=255)
                                         gATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGt                                                                                                       >  1:1873911/1‑148 (MQ=255)
                                          aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTa                                                                                                      <  2:763483/148‑1 (MQ=255)
                                                  tgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATc                                                                                              >  2:3300630/1‑148 (MQ=255)
                                                  tgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATc                                                                                              >  2:691114/1‑148 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            >  1:2596860/1‑148 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            >  2:804474/1‑148 (MQ=255)
                                                        tCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCacacac                                                                                        <  2:1368131/148‑1 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       >  2:2627346/1‑148 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       <  1:102858/148‑1 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    >  1:2863064/1‑148 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    >  1:2909761/1‑148 (MQ=255)
                                                             ccATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGt                                                                                   >  1:3498370/1‑148 (MQ=255)
                                                             ccATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGt                                                                                   <  2:2519155/148‑1 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 >  1:1228072/1‑148 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 >  1:1228067/1‑148 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGAAGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCTCTGACGATATTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 <  1:125550/148‑1 (MQ=255)
                                                                          tGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCAt                                                                      <  1:510237/148‑1 (MQ=255)
                                                                                    cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                            <  2:2578698/148‑1 (MQ=255)
                                                                                    cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                            <  1:1595658/148‑1 (MQ=255)
                                                                                      cgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTAtt                                                          <  1:156721/148‑1 (MQ=255)
                                                                                        aCGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGa                                                        >  1:118280/1‑148 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       >  1:999683/1‑148 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       <  2:2150572/148‑1 (MQ=255)
                                                                                          gTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATc                                                      <  2:3458796/148‑1 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  1:1815894/1‑148 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  2:1097932/1‑148 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  1:442350/1‑148 (MQ=255)
                                                                                             gATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGc                                                   <  1:3343398/148‑1 (MQ=255)
                                                                                               tCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                 >  1:3076585/1‑148 (MQ=255)
                                                                                                cAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGc                                                >  2:3026942/1‑148 (MQ=255)
                                                                                                  aaCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGt                                              >  2:46844/1‑148 (MQ=255)
                                                                                                   aCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTg                                             >  2:2188589/1‑148 (MQ=255)
                                                                                                     cGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCt                                           >  2:1093586/1‑148 (MQ=255)
                                                                                                      gTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                          >  2:2515922/1‑148 (MQ=255)
                                                                                                        tAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtt                                        >  1:2048179/1‑148 (MQ=255)
                                                                                                        tAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtt                                        >  2:1794276/1‑148 (MQ=255)
                                                                                                         aaaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGttt                                       >  1:285902/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  1:1113598/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  2:1349036/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  2:3152193/1‑148 (MQ=255)
                                                                                                                   cgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTAGTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGa                             <  2:3066727/148‑1 (MQ=255)
                                                                                                                     gCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                           >  1:882594/1‑148 (MQ=255)
                                                                                                                     gCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                           <  2:1615534/148‑1 (MQ=255)
                                                                                                                      ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                          >  1:2481737/1‑148 (MQ=255)
                                                                                                                      ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                          <  2:3355027/148‑1 (MQ=255)
                                                                                                                        tttGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGc                        <  1:109845/148‑1 (MQ=255)
                                                                                                                          tGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGt                      <  1:2550483/148‑1 (MQ=255)
                                                                                                                           gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGGTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTa                     <  2:1962687/148‑1 (MQ=255)
                                                                                                                            tCGCTGACGATAGTTTTGTGCTACTGTCCGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAc                    <  2:1467770/148‑1 (MQ=255)
                                                                                                                             cGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACt                   >  1:3130160/1‑148 (MQ=255)
                                                                                                                              gCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTg                  <  1:1016348/148‑1 (MQ=255)
                                                                                                                               cTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTgg                 >  2:2755398/1‑148 (MQ=255)
                                                                                                                                tGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgt                >  1:2370441/1‑148 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               >  1:3216954/1‑148 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               >  1:3217176/1‑148 (MQ=255)
                                                                                                                                  aCGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtgt              <  2:1815894/148‑1 (MQ=255)
                                                                                                                                  aCGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtgt              <  1:3459415/148‑1 (MQ=255)
                                                                                                                                   cGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgtt             <  1:691036/148‑1 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           >  2:2946708/1‑148 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           >  2:678784/1‑148 (MQ=255)
                                                                                                                                      tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTCTTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg          >  1:3306877/1‑148 (MQ=255)
                                                                                                                                      tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg          >  1:1567475/1‑148 (MQ=255)
                                                                                                                                       aGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTCCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTgtgt         <  2:576927/148‑1 (MQ=255)
                                                                                                                                         ttttGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCg       <  1:2622756/148‑1 (MQ=255)
                                                                                                                                              tgCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAaca  <  2:646675/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑