BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,784,871	C→A	35.0%	D211Y (GAT→TAT)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,784,871	0	C	A	35.0%	335.8 / 190.7	220	D211Y (GAT→TAT)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand): ref base C (65/78); new base A (39/38); total (104/116)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 244 total aligned reads displayed.

ACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCG  >  CP009273/1784724‑1785017

aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc                                                                                                                                                    >  1:899509/1‑148 (MQ=255)

aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc                                                                                                                                                    >  2:1035969/1‑148 (MQ=255)

aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc                                                                                                                                                    >  2:666078/1‑148 (MQ=255)

 cGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatca                                                                                                                                                   >  2:2600084/1‑148 (MQ=255)

  ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTcataat                                                                                                                                                  <  1:700584/148‑1 (MQ=255)

   gCCCGTAATCACGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCataatc                                                                                                                                                 <  1:535191/148‑1 (MQ=255)

   gCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCataatc                                                                                                                                                 <  1:536099/148‑1 (MQ=255)

   gCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCataatc                                                                                                                                                 <  1:2007353/148‑1 (MQ=255)

     cgttAAACACGCGTCAGCCCCCGCAGCGACATGTTGTCGTTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTg                                                                                                                                               <  1:298501/145‑1 (MQ=255)

     ccGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTg                                                                                                                                               <  1:122529/148‑1 (MQ=255)

       gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCa                                                                                                                                             <  2:3257261/148‑1 (MQ=255)

       gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCa                                                                                                                                             <  2:3034959/148‑1 (MQ=255)

         aaTCAAGCGTCAGCCCCAGCAGCGACTTGTTGTCGGTATTCATCACCCCCTGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCATCATCATCTGCAAg                                                                                                                                           <  1:321143/148‑1 (MQ=255)

          aTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGa                                                                                                                                          >  2:2416414/1‑148 (MQ=255)

          aTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGa                                                                                                                                          <  1:3245625/148‑1 (MQ=255)

            cAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtg                                                                                                                                        <  2:1116742/148‑1 (MQ=255)

             aaGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtgt                                                                                                                                       <  2:647748/148‑1 (MQ=255)

                cGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAc                                                                                                                                    >  1:1193062/1‑148 (MQ=255)

                cGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAc                                                                                                                                    >  1:409380/1‑148 (MQ=255)

                 gTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAcc                                                                                                                                   <  2:166143/148‑1 (MQ=255)

                    aGCCCCCGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGt                                                                                                                                <  1:68213/148‑1 (MQ=255)

                       ccccGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAAt                                                                                                                             <  2:377647/148‑1 (MQ=255)

                         cagcagCGACATGTTGTCGTTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGa                                                                                                                           <  2:2875468/148‑1 (MQ=255)

                         cagcagCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGa                                                                                                                           >  1:2272745/1‑148 (MQ=255)

                         cagcagCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGa                                                                                                                           <  2:2591285/148‑1 (MQ=255)

                         cagcagCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGa                                                                                                                           <  2:2591203/148‑1 (MQ=255)

                            cagcGACATGTTGTCGGTATTCATCCCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTCACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGa                                                                                                                        <  1:963445/148‑1 (MQ=255)

                               cGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                                                     >  1:1399311/1‑148 (MQ=255)

                               cGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                                                     >  2:710668/1‑148 (MQ=255)

                               cGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                                                     >  2:923695/1‑148 (MQ=255)

                                   aTGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCaa                                                                                                                 <  1:2165046/148‑1 (MQ=255)

                                    tgttgtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCaaa                                                                                                                >  1:3065984/1‑148 (MQ=255)

                                          cGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGc                                                                                                          >  2:3382507/1‑148 (MQ=255)

                                             tATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTaa                                                                                                       <  2:2915813/148‑1 (MQ=255)

                                             tATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTaa                                                                                                       >  1:330910/1‑148 (MQ=255)

                                                 catcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGa                                                                                                   >  2:856141/1‑148 (MQ=255)

                                                      ccccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAc                                                                                              <  2:2429635/148‑1 (MQ=255)

                                                       cccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                                                             <  2:3409857/148‑1 (MQ=255)

                                                       cccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                                                             <  2:891335/148‑1 (MQ=255)

                                                        ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCt                                                                                            >  1:1009198/1‑148 (MQ=255)

                                                        ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCt                                                                                            >  2:2965159/1‑148 (MQ=255)

                                                           tGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtttt                                                                                         <  1:860759/148‑1 (MQ=255)

                                                            gAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTc                                                                                        <  1:655058/148‑1 (MQ=255)

                                                            gAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTc                                                                                        <  1:2416414/148‑1 (MQ=255)

                                                             aGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTcc                                                                                       <  1:3080679/148‑1 (MQ=255)

                                                               cAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                     >  2:2847436/1‑148 (MQ=255)

                                                               cAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                     <  1:2320059/148‑1 (MQ=255)

                                                               cAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                     <  1:3303708/148‑1 (MQ=255)

                                                               cAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                     <  2:1446194/148‑1 (MQ=255)

                                                                  aGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTc                                                                                  >  1:2199889/1‑148 (MQ=255)

                                                                     cGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcgcg                                                                               >  1:1322108/1‑148 (MQ=255)

                                                                        ccGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggcg                                                                            <  2:1981802/148‑1 (MQ=255)

                                                                           tCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgta                                                                         >  2:267899/1‑148 (MQ=255)

                                                                           tCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgta                                                                         >  2:1504379/1‑148 (MQ=255)

                                                                              gCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTGTTCCGACTCGCGGCGgtagta                                                                      <  1:1610999/148‑1 (MQ=255)

                                                                                cATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaaa                                                                    >  1:1439908/1‑148 (MQ=255)

                                                                                 aTTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaaaa                                                                   >  2:1788044/1‑148 (MQ=255)

                                                                                 aTTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaaaa                                                                   <  1:2538448/148‑1 (MQ=255)

                                                                                   tGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAAt                                                                 >  2:1122556/1‑148 (MQ=255)

                                                                                    gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATg                                                                <  2:2273673/148‑1 (MQ=255)

                                                                                     ggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGt                                                               <  1:710668/148‑1 (MQ=255)

                                                                                      gCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGtt                                                              <  2:2943548/148‑1 (MQ=255)

                                                                                      gCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGtt                                                              <  1:2518098/148‑1 (MQ=255)

                                                                                        aaTTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCg                                                            <  2:177696/148‑1 (MQ=255)

                                                                                          ttAACGACGCGTTAAGTTCGACAACATCGATAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATATCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGaa                                                          <  1:2657119/148‑1 (MQ=255)

                                                                                          ttAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGaa                                                          >  2:47496/1‑148 (MQ=255)

                                                                                              cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGa                                                      >  2:592539/1‑148 (MQ=255)

                                                                                              cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGa                                                      <  2:1765747/148‑1 (MQ=255)

                                                                                               gacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAc                                                     <  1:3430720/148‑1 (MQ=255)

                                                                                               gacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAc                                                     <  1:299906/148‑1 (MQ=255)

                                                                                                 cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCa                                                   >  1:2078566/1‑148 (MQ=255)

                                                                                                   cgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                 <  1:1240430/148‑1 (MQ=255)

                                                                                                     gTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgc                                               >  2:955202/1‑148 (MQ=255)

                                                                                                      tACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgcg                                              >  2:1002027/1‑148 (MQ=255)

                                                                                                          tGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                          >  1:1330892/1‑148 (MQ=255)

                                                                                                          tGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                          <  2:1085577/148‑1 (MQ=255)

                                                                                                                aacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt                                    <  2:2007773/148‑1 (MQ=255)

                                                                                                                aacaTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt                                    <  2:2199889/148‑1 (MQ=255)

                                                                                                                 acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTg                                   <  1:93339/148‑1 (MQ=255)

                                                                                                                 acaTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTg                                   <  2:518665/148‑1 (MQ=255)

                                                                                                                         aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCcaca                           >  1:518512/1‑148 (MQ=255)

                                                                                                                          aaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                          >  2:2478065/1‑148 (MQ=255)

                                                                                                                          aaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                          >  1:2105373/1‑148 (MQ=255)

                                                                                                                          aaCCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                          >  1:997498/1‑148 (MQ=255)

                                                                                                                            ccAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGc                        >  1:2860168/1‑148 (MQ=255)

                                                                                                                              agagACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAATCGCAGATGACTTGGTGCCACACGCAt                      >  1:689073/1‑148 (MQ=255)

                                                                                                                                  aCGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGc                  >  1:2180095/1‑148 (MQ=255)

                                                                                                                                    ggTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCAt                <  1:1522402/148‑1 (MQ=255)

                                                                                                                                    ggTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCAt                <  1:2015948/148‑1 (MQ=255)

                                                                                                                                     gTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATc               >  2:1321517/1‑148 (MQ=255)

                                                                                                                                        tttGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgcg            >  2:112870/1‑148 (MQ=255)

                                                                                                                                        tttGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgcg            >  2:2639838/1‑148 (MQ=255)

                                                                                                                                         ttGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCgcgc           >  1:108381/1‑148 (MQ=255)

                                                                                                                                         ttGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCgcgc           >  1:1686156/1‑148 (MQ=255)

                                                                                                                                         ttGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCgcgc           >  1:3472080/1‑148 (MQ=255)

                                                                                                                                            tCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTg        >  1:2773663/1‑148 (MQ=255)

                                                                                                                                             ccTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTgg       <  2:2766336/148‑1 (MQ=255)

                                                                                                                                               tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCt     <  1:1916885/148‑1 (MQ=255)

                                                                                                                                                  tcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccg  <  1:3168281/148‑1 (MQ=255)

                                                                                                                                                  tcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccg  <  1:71767/148‑1 (MQ=255)

ACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCG  >  CP009273/1784724‑1785017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`