Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,818,738 C→A 94.3% D153Y (GAC→TAC)  ves ← cold‑ and stress‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,818,7380CA94.3% 498.5 / 19.7 176D153Y (GAC→TAC) vescold‑ and stress‑inducible protein
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base A (100/66);  total (104/72)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 179 total aligned reads displayed.

CCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATC  >  CP009273/1818594‑1818882
                                                                                                                                                |                                                                                                                                                
ccGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGt                                                                                                                                               <  2:2769724/148‑1 (MQ=255)
  gctgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAATGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAg                                                                                                                                             <  2:2885138/148‑1 (MQ=255)
  gctgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAg                                                                                                                                             <  2:1135041/148‑1 (MQ=255)
   ctgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGt                                                                                                                                            >  1:751181/1‑148 (MQ=255)
    tgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTg                                                                                                                                           >  1:1373456/1‑148 (MQ=255)
      ctgtCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAg                                                                                                                                         <  2:2130560/145‑1 (MQ=255)
      ctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAg                                                                                                                                         <  2:3318950/148‑1 (MQ=255)
      ctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAg                                                                                                                                         >  1:3227097/1‑148 (MQ=255)
      ctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAg                                                                                                                                         <  2:2431589/148‑1 (MQ=255)
        ttCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCa                                                                                                                                       >  1:2096378/1‑148 (MQ=255)
             gTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTa                                                                                                                                  <  2:3090927/148‑1 (MQ=255)
             gTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTa                                                                                                                                  <  2:1259093/148‑1 (MQ=255)
                  tATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGcc                                                                                                                             >  1:3078637/1‑148 (MQ=255)
                   aTGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGccc                                                                                                                            <  2:3177113/148‑1 (MQ=255)
                   aTGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGccc                                                                                                                            >  1:3359428/1‑148 (MQ=255)
                   aTGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGccc                                                                                                                            <  2:1386543/148‑1 (MQ=255)
                    tGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCa                                                                                                                           >  2:2406772/1‑148 (MQ=255)
                        aTTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTg                                                                                                                       >  2:1635308/1‑148 (MQ=255)
                         ttACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGc                                                                                                                      <  1:600565/148‑1 (MQ=255)
                          tACTGAACTTGATCCGGAGAATGACAAGCGAGCAAGTTGTTTTCACTGAACAACAGTTTACCTTGGGGTTGTAAAAGCCGAAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGcc                                                                                                                     <  1:829922/148‑1 (MQ=255)
                              gAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGc                                                                                                                 >  2:1625572/1‑148 (MQ=255)
                               aaCTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGcc                                                                                                                >  2:878914/1‑148 (MQ=255)
                                   tGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGt                                                                                                            <  1:2679236/148‑1 (MQ=255)
                                     aTCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTa                                                                                                          <  1:750395/148‑1 (MQ=255)
                                     aTCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTa                                                                                                          <  1:857484/148‑1 (MQ=255)
                                     aTCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTa                                                                                                          <  1:1971889/148‑1 (MQ=255)
                                      tCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTaa                                                                                                         <  1:3434689/148‑1 (MQ=255)
                                      tCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTaa                                                                                                         >  2:1492222/1‑148 (MQ=255)
                                        cGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATc                                                                                                       <  1:1744517/148‑1 (MQ=255)
                                         ggCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATca                                                                                                      >  1:3158964/1‑148 (MQ=255)
                                           cGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATcaca                                                                                                    >  1:266561/1‑148 (MQ=255)
                                             aaTGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACaaa                                                                                                  >  1:680472/1‑148 (MQ=255)
                                             aaTGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACaaa                                                                                                  >  1:675556/1‑148 (MQ=255)
                                               tGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAaca                                                                                                <  2:1957199/148‑1 (MQ=255)
                                               tGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAaca                                                                                                <  2:1956147/148‑1 (MQ=255)
                                                gACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAacac                                                                                               >  2:2672765/1‑148 (MQ=255)
                                                 aCCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAAcacc                                                                                              <  2:1417096/148‑1 (MQ=255)
                                                     gCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCAccc                                                                                          >  2:2504669/1‑148 (MQ=255)
                                                      cGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCAcccc                                                                                         >  2:1763950/1‑148 (MQ=255)
                                                        aGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCccgc                                                                                       <  1:170243/148‑1 (MQ=255)
                                                        aGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCccgc                                                                                       <  1:170232/148‑1 (MQ=255)
                                                         gCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCccgcc                                                                                      >  2:535346/1‑148 (MQ=255)
                                                            aGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCcgcg                                                                                   >  2:1540246/1‑148 (MQ=255)
                                                            aGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCcgcg                                                                                   <  2:661612/148‑1 (MQ=255)
                                                                gATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAg                                                                               >  1:3462526/1‑148 (MQ=255)
                                                                 aTTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGc                                                                              <  1:3377579/148‑1 (MQ=255)
                                                                  tttCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGcc                                                                             >  2:885986/1‑148 (MQ=255)
                                                                   ttCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCa                                                                            >  1:1119136/1‑148 (MQ=255)
                                                                   ttCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCa                                                                            >  1:1119180/1‑148 (MQ=255)
                                                                    tCACTGAACAACAGTTTACCTTGCGGTTGTAAAANCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTANTGNGCAATTTATCGCCCAGTTGCCACGNCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCaa                                                                           <  1:1664575/148‑1 (MQ=255)
                                                                     cACTGACCTTCAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCTGCACGCGCCCTGGTAGGTAGTGAGCAATTTTTCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCaaaa                                                                          <  2:576129/148‑1 (MQ=255)
                                                                      aCTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAg                                                                         >  1:2378037/1‑148 (MQ=255)
                                                                       cTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAgg                                                                        >  1:405035/1‑148 (MQ=255)
                                                                         gAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgta                                                                      >  2:36462/1‑148 (MQ=255)
                                                                          aacaacAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtag                                                                     >  1:3177748/1‑148 (MQ=255)
                                                                           acaacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtagt                                                                    >  1:1185731/1‑148 (MQ=255)
                                                                           acaacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtagt                                                                    >  1:2860271/1‑148 (MQ=255)
                                                                            caacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtagta                                                                   >  2:1017870/1‑148 (MQ=255)
                                                                            caacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgtagta                                                                   >  1:411615/1‑148 (MQ=255)
                                                                             tagtttttACCTTTGGGTTGTAAACGCCGCAGGGTGTGGCGGCCATAGAACGAGCACGCGCCCTGGTAGGTTGTGAGCAATTTATCGCCGAGTTGCCACGGCCCGTTTATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaa                                                                  <  2:1874139/143‑1 (MQ=255)
                                                                                  tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             >  1:3465088/1‑148 (MQ=255)
                                                                                  tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             >  2:693779/1‑148 (MQ=255)
                                                                                  tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             <  1:1930039/148‑1 (MQ=255)
                                                                                  tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             >  1:2428217/1‑148 (MQ=255)
                                                                                       cTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTc                                                        <  2:671182/148‑1 (MQ=255)
                                                                                       cTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTc                                                        <  1:864159/148‑1 (MQ=255)
                                                                                        ttGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCg                                                       >  1:3244674/1‑148 (MQ=255)
                                                                                           cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCa                                                    <  1:123869/148‑1 (MQ=255)
                                                                                             gTTGTAAAATCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTTGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAAt                                                  <  2:3414218/148‑1 (MQ=255)
                                                                                               tGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTc                                                >  1:1440006/1‑148 (MQ=255)
                                                                                                 tAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTg                                              >  2:3225322/1‑148 (MQ=255)
                                                                                                  aaaaGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGa                                             >  1:425911/1‑148 (MQ=255)
                                                                                                    aaGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACt                                           <  1:2198949/148‑1 (MQ=255)
                                                                                                    aaGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACt                                           >  2:1458569/1‑148 (MQ=255)
                                                                                                      gCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACttt                                         <  1:1492222/148‑1 (MQ=255)
                                                                                                      gCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACttt                                         <  1:2334555/148‑1 (MQ=255)
                                                                                                       ccGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTc                                        >  1:2765502/1‑148 (MQ=255)
                                                                                                        cGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCg                                       <  1:2688250/148‑1 (MQ=255)
                                                                                                         gCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGc                                      <  1:447462/148‑1 (MQ=255)
                                                                                                         gCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGc                                      <  1:2634627/148‑1 (MQ=255)
                                                                                                          cAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGcc                                     <  2:2098407/148‑1 (MQ=255)
                                                                                                           aGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCt                                    >  1:140695/1‑148 (MQ=255)
                                                                                                            gggTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCtt                                   <  1:2504669/148‑1 (MQ=255)
                                                                                                             ggTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTa                                  <  1:854458/148‑1 (MQ=255)
                                                                                                                    cttccATCGAACCAGCTCGCGCCCTGGTAGGTAGTGAGCTATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACAt                           <  1:355326/145‑1 (MQ=255)
                                                                                                                    ctgccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACAt                           <  1:355457/148‑1 (MQ=255)
                                                                                                                     tgccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATc                          >  2:2950646/1‑148 (MQ=255)
                                                                                                                      gccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATcc                         >  1:244839/1‑148 (MQ=255)
                                                                                                                         aTCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAgg                      <  2:1958260/148‑1 (MQ=255)
                                                                                                                         aTCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAgg                      <  1:2937223/148‑1 (MQ=255)
                                                                                                                          tCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGc                     >  1:212027/1‑148 (MQ=255)
                                                                                                                           cGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCg                    >  2:260608/1‑148 (MQ=255)
                                                                                                                           cGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCg                    >  2:267169/1‑148 (MQ=255)
                                                                                                                           cGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCg                    >  2:3498823/1‑148 (MQ=255)
                                                                                                                           cGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCg                    >  2:2303271/1‑148 (MQ=255)
                                                                                                                              aCCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCgtgt                 >  1:2738237/1‑148 (MQ=255)
                                                                                                                                  gCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCatg             >  2:3027711/1‑148 (MQ=255)
                                                                                                                                         cccTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGa      >  1:975531/1‑148 (MQ=255)
                                                                                                                                          ccTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGaa     <  2:1373456/148‑1 (MQ=255)
                                                                                                                                          ccTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGaa     >  1:631228/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATC  >  CP009273/1818594‑1818882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑