Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,829,319 C→A 100% I85I (ATC→ATA)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,829,3190CA99.0% 640.9 / ‑2.0 201I85I (ATC→ATA) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (81/117);  total (84/117)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT  >  CP009273/1829175‑1829462
                                                                                                                                                |                                                                                                                                               
cAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAAcc                                                                                                                                              <  2:46767/148‑1 (MQ=255)
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                             >  1:3089095/1‑148 (MQ=255)
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                             <  2:1765159/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                            <  1:3303835/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                            <  1:1064072/148‑1 (MQ=255)
    cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                          <  2:2944979/148‑1 (MQ=255)
       aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCGGCGTTTTTGATAAACATTGCCa                                                                                                                                       >  1:1004335/1‑148 (MQ=255)
       aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTAAGTCTTAAGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCa                                                                                                                                       >  1:1004305/1‑148 (MQ=255)
        gCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                      <  2:1130551/148‑1 (MQ=255)
         cGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                     >  2:537141/1‑148 (MQ=255)
          gTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATg                                                                                                                                    <  1:1356538/148‑1 (MQ=255)
           ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTGCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                   >  2:2633519/1‑148 (MQ=255)
            tGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCg                                                                                                                                  <  1:929784/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:1906391/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:1870809/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                 <  2:131049/148‑1 (MQ=255)
                gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                              <  2:2565158/148‑1 (MQ=255)
                 cgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCt                                                                                                                             <  2:1547441/148‑1 (MQ=255)
                  gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTg                                                                                                                            <  2:1035236/148‑1 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATGACCTTTGCCAATGCGTCGCTGtt                                                                                                                          >  1:1241657/1‑148 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                          <  2:2903871/148‑1 (MQ=255)
                    ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGGTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                          >  1:2458105/1‑148 (MQ=255)
                     tGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGttt                                                                                                                         <  2:1971223/148‑1 (MQ=255)
                          ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcgc                                                                                                                    <  1:1830268/148‑1 (MQ=255)
                                  cAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                            >  1:3410400/1‑148 (MQ=255)
                                  cAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                            >  1:2284450/1‑148 (MQ=255)
                                    aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggc                                                                                                          >  2:2929766/1‑148 (MQ=255)
                                    aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggc                                                                                                          >  1:2275840/1‑148 (MQ=255)
                                        ggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCt                                                                                                      >  2:172176/1‑148 (MQ=255)
                                         gTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                     <  1:1876089/148‑1 (MQ=255)
                                         gTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                     <  1:2654874/148‑1 (MQ=255)
                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGc                                                                                                   <  2:2843377/148‑1 (MQ=255)
                                              gAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATTCGTCGCTGTTTGTCGCGTTCTGGGGCGGCTTGCTGt                                                                                                >  1:1446790/1‑148 (MQ=255)
                                               aaCGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTc                                                                                               >  2:2745201/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  2:1660117/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  2:2457241/1‑148 (MQ=255)
                                                   cTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                           >  1:3503841/1‑148 (MQ=255)
                                                    tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGa                                                                                          <  1:3153668/148‑1 (MQ=255)
                                                     ttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAc                                                                                         >  1:2341922/1‑148 (MQ=255)
                                                      tATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                        <  1:2604619/148‑1 (MQ=255)
                                                      tATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                        <  1:367343/148‑1 (MQ=255)
                                                       aTTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCa                                                                                       <  2:2797174/148‑1 (MQ=255)
                                                        ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGTGCGGCTTGCTGTCGTGGACCAg                                                                                      <  2:386164/148‑1 (MQ=255)
                                                        ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAg                                                                                      <  1:280619/148‑1 (MQ=255)
                                                         tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                     <  1:1694506/148‑1 (MQ=255)
                                                          cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGtt                                                                                    >  1:2955561/1‑148 (MQ=255)
                                                             tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                 >  1:3121810/1‑148 (MQ=255)
                                                             tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                 >  1:2941603/1‑148 (MQ=255)
                                                              cTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGAt                                                                                <  1:2288451/148‑1 (MQ=255)
                                                                tACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATgg                                                                              >  2:690648/1‑148 (MQ=255)
                                                                 aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                             <  2:2133152/148‑1 (MQ=255)
                                                                 aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                             <  1:1047388/148‑1 (MQ=255)
                                                                            gcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                  >  2:2641969/1‑148 (MQ=255)
                                                                              ggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                <  2:626097/148‑1 (MQ=255)
                                                                              ggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                <  2:2391122/148‑1 (MQ=255)
                                                                                cAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtgtg                                                              <  2:772182/148‑1 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                           <  2:3089095/148‑1 (MQ=255)
                                                                                      tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttttt                                                        <  1:1617900/148‑1 (MQ=255)
                                                                                        tcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTa                                                      >  2:2133981/1‑148 (MQ=255)
                                                                                        tcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGACGCTGTGCTTTTTTa                                                      >  2:3153116/1‑148 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                   <  2:3391124/134‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGTGCTGTGCTTTTTTAt                                                     <  1:68718/148‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTAt                                                     <  1:68478/148‑1 (MQ=255)
                                                                                         cGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGAGCTGTGCTTTTTTAt                                                     >  2:2359063/1‑148 (MQ=255)
                                                                                           ttCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                   >  1:1181712/1‑148 (MQ=255)
                                                                                           ttCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                   >  1:556064/1‑148 (MQ=255)
                                                                                                  tgatgaTTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGt                                            >  2:2748533/1‑148 (MQ=255)
                                                                                                   gatgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                           >  1:231432/1‑148 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:3410400/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:2284450/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGAGCTGTGCTTTTTTATCGCCAGAGTGa                                          <  2:522154/148‑1 (MQ=255)
                                                                                                        ttttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATggg                                      <  2:149922/148‑1 (MQ=255)
                                                                                                         tttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGGTTTTTTATAGCCAGAGTGATGGGc                                     >  1:3325647/1‑148 (MQ=255)
                                                                                                         tttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCGGGGCTTTTTTATCGCCAGAGGGATGGGc                                     >  1:3326296/1‑148 (MQ=255)
                                                                                                               ggCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGaa                               >  2:1537194/1‑148 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              <  1:3205166/148‑1 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              <  1:2633519/148‑1 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                              >  2:2595241/1‑148 (MQ=255)
                                                                                                                 ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgt                             >  1:2022312/1‑148 (MQ=255)
                                                                                                                  cATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                            <  1:537141/148‑1 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  2:2753079/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGTGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  2:425474/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGGCGGCGCGGCGCTGTGCTTTTTTTTAGCCAGAGTGTTGGGCCGCGAAgtgg                           >  1:2405430/1‑148 (MQ=255)
                                                                                                                   aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                           >  1:445522/1‑148 (MQ=255)
                                                                                                                    gcgctgcACCGCTACCTGCGTTTTTGAGAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                          <  1:797250/146‑1 (MQ=255)
                                                                                                                     tgctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggtg                         >  2:1699169/1‑148 (MQ=255)
                                                                                                                     tgctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggtg                         >  1:2868444/1‑148 (MQ=255)
                                                                                                                      gctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGtggtgg                        <  2:2038019/148‑1 (MQ=255)
                                                                                                                            ccGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAt                  <  2:1004333/148‑1 (MQ=255)
                                                                                                                             cGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAAAtt                 >  1:2247499/1‑148 (MQ=255)
                                                                                                                             cGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGCGGCGGCTTTCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCAGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAtt                 >  2:45314/1‑148 (MQ=255)
                                                                                                                                tACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAc              <  1:1795844/148‑1 (MQ=255)
                                                                                                                                 aCCTGCGTTTTTGATAACCTTTGCCACTGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGTCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAcc             <  2:3016615/148‑1 (MQ=255)
                                                                                                                                     gCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa         >  2:1731784/1‑148 (MQ=255)
                                                                                                                                     gCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGACGTGGACCAGTTCGATGGCAGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAATTTCTCCGGCa         >  2:2307837/1‑148 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc     <  1:1969598/148‑1 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc     <  1:277577/148‑1 (MQ=255)
                                                                                                                                          tttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc    <  2:3254109/148‑1 (MQ=255)
                                                                                                                                          tttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc    <  2:1451529/148‑1 (MQ=255)
                                                                                                                                            tGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGt  >  1:2007056/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGT  >  CP009273/1829175‑1829462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑