Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,832,413 C→A 100% L315L (CTC→CTA)  ynjC → inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,832,4130CA100.0% 583.0 / ‑6.7 181L315L (CTC→CTA) ynjCinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (87/92);  total (89/92)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

TTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCC  >  CP009273/1832268‑1832555
                                                                                                                                                 |                                                                                                                                              
ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                              >  2:1940138/1‑148 (MQ=255)
ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                              >  2:164920/1‑148 (MQ=255)
ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                              >  1:2486428/1‑148 (MQ=255)
      gcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAg                                                                                                                                        <  2:2468054/148‑1 (MQ=255)
       cgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                       <  1:3378493/148‑1 (MQ=255)
       cgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                       <  1:1160307/148‑1 (MQ=255)
       cgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                       >  2:167028/1‑148 (MQ=255)
       cgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                       >  2:2966473/1‑148 (MQ=255)
         tAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCt                                                                                                                                     >  2:3372098/1‑148 (MQ=255)
         tAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCt                                                                                                                                     >  2:2524084/1‑148 (MQ=255)
         tAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCt                                                                                                                                     <  2:283256/148‑1 (MQ=255)
           gATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                   >  2:2618270/1‑148 (MQ=255)
           gATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                   <  1:2456253/148‑1 (MQ=255)
           gATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                   <  1:1565500/148‑1 (MQ=255)
           gATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                   >  2:2326839/1‑148 (MQ=255)
           gATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCGCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                   >  1:592510/1‑148 (MQ=255)
            aTGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGAc                                                                                                                                  >  2:2559212/1‑148 (MQ=255)
             tGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACa                                                                                                                                 <  1:1520599/148‑1 (MQ=255)
               gCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAAt                                                                                                                               <  2:1229425/148‑1 (MQ=255)
               gCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAAt                                                                                                                               <  2:1229782/148‑1 (MQ=255)
                   tCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGGTGTGGGGGGTTCTGCTGGCGATCCTCGCGGATCAGTCGGCGATCAATAGTGAAGCGCGAATCAACAGCCTGACAATgggg                                                                                                                           >  1:101382/1‑148 (MQ=255)
                   tCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATgggg                                                                                                                           <  1:264942/148‑1 (MQ=255)
                        aGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCGGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCtggt                                                                                                                      >  2:819456/1‑148 (MQ=255)
                          ccTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCtggtgg                                                                                                                    >  2:3490971/1‑148 (MQ=255)
                               cACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATAAACAGCCTGACAATGGGGCTGGTGGCGACa                                                                                                               >  2:1026040/1‑148 (MQ=255)
                                    cTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCAt                                                                                                          <  1:2801841/148‑1 (MQ=255)
                                      ttATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCg                                                                                                        >  2:640679/1‑148 (MQ=255)
                                       tagtGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGc                                                                                                       <  2:3327256/145‑1 (MQ=255)
                                        aTTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCt                                                                                                      <  1:1230657/148‑1 (MQ=255)
                                        aTTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCt                                                                                                      <  1:2978041/148‑1 (MQ=255)
                                         ttGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCtt                                                                                                     >  1:2920087/1‑148 (MQ=255)
                                         ttGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCtt                                                                                                     >  1:2920184/1‑148 (MQ=255)
                                         ttGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCtt                                                                                                     <  2:3037604/148‑1 (MQ=255)
                                         ttGCCGGGCAATACGCTGGCGATTTTTTTAACCATAACCGGGGTGGTGTGTGTGGTTCTGCTGGCGATCCAAGCGGATCAGTCGACGATCAATAGTTAATCGCTAATCAACAGCCGGAAAAAGGGGCTGGTGGCGACATTCATCGCtt                                                                                                     >  1:644636/1‑148 (MQ=255)
                                          tGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGGGTGCGGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCttt                                                                                                    >  2:1144464/1‑148 (MQ=255)
                                              gggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCCACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTc                                                                                                <  2:713468/148‑1 (MQ=255)
                                              gggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTc                                                                                                <  2:839807/148‑1 (MQ=255)
                                              gggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTc                                                                                                <  2:207328/148‑1 (MQ=255)
                                               ggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATAAACAGCCTGACAATTGGGCTGGTGGCGACATTCATAGCTTTGCTcc                                                                                               >  1:867730/1‑148 (MQ=255)
                                                 cAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTg                                                                                             <  2:3123004/148‑1 (MQ=255)
                                                 cAATACGCGGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTg                                                                                             <  2:3020862/148‑1 (MQ=255)
                                                    tACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTa                                                                                          <  2:2420290/148‑1 (MQ=255)
                                                    tACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCGGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCGGGTGGCGACATTCATCGCTTTGCTCCTGTTa                                                                                          >  2:1419037/1‑148 (MQ=255)
                                                    tACGCTGGCGATTTTTTTACCCTTAACCGGGGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTAATCGCTTTGCTCCTGTTa                                                                                          >  1:1446167/1‑148 (MQ=255)
                                                       gCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActg                                                                                       <  2:1503491/148‑1 (MQ=255)
                                                       gCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActg                                                                                       <  1:1961002/148‑1 (MQ=255)
                                                       gCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActg                                                                                       <  1:364301/148‑1 (MQ=255)
                                                          ggCGATTTTTTTACCCTGAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgctg                                                                                    <  2:597376/148‑1 (MQ=255)
                                                           gCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCtgt                                                                                   >  1:1383092/1‑148 (MQ=255)
                                                             gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGACTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTgg                                                                                 >  2:1428747/1‑148 (MQ=255)
                                                               tttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                               <  1:584576/148‑1 (MQ=255)
                                                               tttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                               <  1:285971/148‑1 (MQ=255)
                                                                ttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTg                                                                              >  2:3229709/1‑148 (MQ=255)
                                                                  ttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGa                                                                            <  1:3489970/148‑1 (MQ=255)
                                                                  tttgACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGa                                                                            <  2:647476/148‑1 (MQ=255)
                                                                    ttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAAt                                                                          <  2:1469599/148‑1 (MQ=255)
                                                                          ttAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGc                                                                    >  1:1943730/1‑148 (MQ=255)
                                                                          ttAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGc                                                                    >  1:904518/1‑148 (MQ=255)
                                                                           tAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGcc                                                                   >  1:3061901/1‑148 (MQ=255)
                                                                               cGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                               >  1:732650/1‑148 (MQ=255)
                                                                               cGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                               >  1:2493911/1‑148 (MQ=255)
                                                                               cGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                               >  1:2494098/1‑148 (MQ=255)
                                                                                 gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCg                                                             >  1:127068/1‑148 (MQ=255)
                                                                                 gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCg                                                             <  1:488639/148‑1 (MQ=255)
                                                                                 gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCg                                                             >  1:1369046/1‑148 (MQ=255)
                                                                                      cTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGcc                                                        <  2:743177/148‑1 (MQ=255)
                                                                                          gtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGtt                                                    >  2:2923810/1‑148 (MQ=255)
                                                                                          gtgtgGGTCTGCGGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGtt                                                    <  1:1938578/148‑1 (MQ=255)
                                                                                              ggTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGCAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTgg                                                <  2:917220/148‑1 (MQ=255)
                                                                                               gTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGc                                               <  1:1962377/148‑1 (MQ=255)
                                                                                                 tCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTa                                             >  2:2573936/1‑148 (MQ=255)
                                                                                                  ctgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAt                                            <  2:2478494/148‑1 (MQ=255)
                                                                                                  ctgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAt                                            >  1:578082/1‑148 (MQ=255)
                                                                                                        gCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGc                                      >  1:1388886/1‑148 (MQ=255)
                                                                                                        gCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGc                                      >  1:215026/1‑148 (MQ=255)
                                                                                                         cGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGcc                                     <  2:2522314/148‑1 (MQ=255)
                                                                                                           aTCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCa                                   >  1:827817/1‑148 (MQ=255)
                                                                                                           aTCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCa                                   >  1:1325647/1‑148 (MQ=255)
                                                                                                           aTCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCa                                   >  1:220291/1‑148 (MQ=255)
                                                                                                             ccTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAtt                                 <  1:1365861/148‑1 (MQ=255)
                                                                                                             ccTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAtt                                 >  2:738849/1‑148 (MQ=255)
                                                                                                              cTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAttt                                <  1:327482/148‑1 (MQ=255)
                                                                                                                    gATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                          >  2:2913153/1‑148 (MQ=255)
                                                                                                                    gATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                          <  1:1355964/148‑1 (MQ=255)
                                                                                                                     aTCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAcc                         <  1:2317332/148‑1 (MQ=255)
                                                                                                                     aTCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAcc                         >  2:2380655/1‑148 (MQ=255)
                                                                                                                     aTCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGATGCCCATTTTATTAcc                         <  2:461277/148‑1 (MQ=255)
                                                                                                                         gTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGct                     <  1:2524084/148‑1 (MQ=255)
                                                                                                                         gTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGct                     >  2:446312/1‑148 (MQ=255)
                                                                                                                          tCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctc                    <  1:1044892/148‑1 (MQ=255)
                                                                                                                              cgaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGcc                >  2:2649846/1‑148 (MQ=255)
                                                                                                                                  cAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtg            >  2:2483163/1‑148 (MQ=255)
                                                                                                                                  cAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtg            >  1:3507011/1‑148 (MQ=255)
                                                                                                                                  cAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtg            <  1:2913153/148‑1 (MQ=255)
                                                                                                                                   aaTAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtgg           <  1:3229709/148‑1 (MQ=255)
                                                                                                                                    aTAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggt          <  2:2486970/148‑1 (MQ=255)
                                                                                                                                        tGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCg      >  2:1371645/1‑148 (MQ=255)
                                                                                                                                          aaGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCggg    <  2:1006588/148‑1 (MQ=255)
                                                                                                                                           aGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc   >  2:1551363/1‑148 (MQ=255)
                                                                                                                                           aGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc   <  1:819456/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCC  >  CP009273/1832268‑1832555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑