Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,836,192 T→A 100% I67I (ATA→ATT)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1920TA98.3% 562.9 / ‑0.1 178I67I (ATA→ATT) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (80/95);  total (82/96)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG  >  CP009273/1836048‑1836336
                                                                                                                                                |                                                                                                                                                
tggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAtt                                                                                                                                               >  2:2735444/1‑148 (MQ=255)
    ctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCtt                                                                                                                                           <  1:2610341/148‑1 (MQ=255)
       gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCACCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                        <  1:1767570/148‑1 (MQ=255)
       gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGGATTGGTTTAAGCGCCGCCAGACCAGCGGGTTAGTGGTAATGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTACTTAgg                                                                                                                                        >  1:1057130/1‑148 (MQ=255)
       gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACAGTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                        <  1:3381250/148‑1 (MQ=255)
         gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                      >  2:2524476/1‑148 (MQ=255)
         gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                      <  1:548892/148‑1 (MQ=255)
            cACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGGTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTa                                                                                                                                   >  2:1305041/1‑148 (MQ=255)
                tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTa                                                                                                                               <  1:631104/148‑1 (MQ=255)
                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTTCGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTAc                                                                                                                              >  2:1650952/1‑148 (MQ=255)
                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAc                                                                                                                              <  2:172106/148‑1 (MQ=255)
                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAc                                                                                                                              <  2:602442/148‑1 (MQ=255)
                 gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCATCGGCGTTAATTAc                                                                                                                              >  1:2612667/1‑148 (MQ=255)
                     agGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGCTTTAAGCGCCCCCAGCCCAGCGGATTAGTGGTAAGGGTTTTGTGCTCACGCGGGCCTTTCAGCAGAATTCCATCGGCTGTAATTACCGcc                                                                                                                          >  1:2523863/1‑148 (MQ=255)
                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                                        <  2:1792099/148‑1 (MQ=255)
                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                                        <  1:1937519/148‑1 (MQ=255)
                       gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                                        <  1:1940432/148‑1 (MQ=255)
                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGa                                                                                                                  <  2:936278/148‑1 (MQ=255)
                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGa                                                                                                                  <  1:2365951/148‑1 (MQ=255)
                              aaTATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaa                                                                                                                 <  1:3165982/148‑1 (MQ=255)
                               atatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaat                                                                                                                <  2:2184710/148‑1 (MQ=255)
                                 atCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaataa                                                                                                              <  2:3464117/148‑1 (MQ=255)
                                   ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTTCAGCAGAAATCCTTAGGCTTTAATTACCGCCCCATCAGAATAAtt                                                                                                            >  1:295693/1‑148 (MQ=255)
                                    cGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAttt                                                                                                           <  2:2005936/148‑1 (MQ=255)
                                      cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                                                                         <  2:2911842/148‑1 (MQ=255)
                                       tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                                                                        <  1:2177162/148‑1 (MQ=255)
                                        ggCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                                       <  2:1244855/148‑1 (MQ=255)
                                         gCCCCTGCTGACATTCCATTATTAGAGGCGTGTTTGGCTTTACGCGCCGCCCGACCCGCGGATTAGTGCTAAGGGGTTTGTCATCACGCTGGGCTTGCAGCAGAATTACATCGGCTTTAATTAACGCCCCGTCAGTATAATTTTGATc                                                                                                      >  1:1587096/1‑148 (MQ=255)
                                          ccccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATcc                                                                                                     <  1:390904/148‑1 (MQ=255)
                                            ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                                   >  2:1451007/1‑148 (MQ=255)
                                            ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                                   <  2:3490820/148‑1 (MQ=255)
                                              tgctgACATTCCATTATTAGAGGCGTTTATGGCTTTTCGCGCCGCCACACCAGCGGATTAGTTCTAATGGTTTTTTGATCACGCGGGCATTTAATCAGAATTACTTCGGCTTTAATTTACGCCCCTTCAGAATAAATTTTATTCTGAt                                                                                                 >  1:1578908/1‑148 (MQ=255)
                                                ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                               <  1:2735444/148‑1 (MQ=255)
                                                ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                               <  1:3501544/148‑1 (MQ=255)
                                                  gACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCACCGGCTTAGTGCTAAGGGGTTTTTCATCAAGCTGGCATTGCAGCAGAAATCCTTCGGCTTTAATTAACGCCCCTTCAGAATAAATTTGATACTGATAAAc                                                                                             >  1:1118968/1‑148 (MQ=255)
                                                   aCATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCGAAGGGTTTTGTCATCACGCAGGCATTGCCGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACg                                                                                            >  2:2348233/1‑148 (MQ=255)
                                                    cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                           <  2:1527022/148‑1 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                         <  2:2938842/148‑1 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                         <  2:788973/148‑1 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                         <  2:2938870/148‑1 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTAATCAAGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACGCCCCATCAGAATAATTTTTATCCTGATAAACgcag                                                                                         >  2:7728/1‑148 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCACCGGATTTGTGCTAAGGGTTTTGTCATCACGCTGGCATTTCAGCAGAATTACTTCGGCTTTAATTACCGCCCCGTCAGAATAATTTTGATCCTGATAAACgcag                                                                                         >  1:857469/1‑148 (MQ=255)
                                                       tCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCCGACCCGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAATCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATACAGATAAACgcagc                                                                                        >  2:1946221/1‑148 (MQ=255)
                                                       tCCATTATTAGAGGCGTGTATGGCTTTACGCGCCCCCAGACCAGCGGCTTAGTGCTAAGGGTTTTGTCCTCACGCGGGCATTGCAGCAGAATTCCGTCGGCTTTAATTACCGCCCCGTCAGAATAATTTTGATCCTGATAAACgcagc                                                                                        >  2:1246042/1‑148 (MQ=255)
                                                        ccATTATTAGAGGCGTTTATGGGTTTAAGCGCCGCCAGACCCGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCCTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAAAgcagca                                                                                       >  2:473149/1‑148 (MQ=255)
                                                         cATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCAc                                                                                      <  1:1145300/148‑1 (MQ=255)
                                                         cATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCACCGGATTAGTGCTAAGGGTTTTGTCATCACCCTGGCATTTCAGCAGAATTCCATAGGCGTTAATTACCGCCCCATCAGAAAAATTTTGATCCGGATAAAAGCAGCAc                                                                                      >  2:3257304/1‑148 (MQ=255)
                                                           ttattaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTg                                                                                    <  2:904920/148‑1 (MQ=255)
                                                             attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAg                                                                                  <  2:1129640/148‑1 (MQ=255)
                                                              ttaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                                                                                 <  1:3354624/148‑1 (MQ=255)
                                                                agagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTAc                                                                               <  2:1408129/148‑1 (MQ=255)
                                                                agagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTAc                                                                               <  2:2734432/148‑1 (MQ=255)
                                                                 gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                              <  2:893752/148‑1 (MQ=255)
                                                                 gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                              <  2:2603806/148‑1 (MQ=255)
                                                                  agGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAg                                                                             <  1:2573912/148‑1 (MQ=255)
                                                                    gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                           >  2:2392233/1‑148 (MQ=255)
                                                                     cGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGc                                                                          <  2:1739456/148‑1 (MQ=255)
                                                                       tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                        >  1:2305653/1‑148 (MQ=255)
                                                                        ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                                       >  2:2043780/1‑148 (MQ=255)
                                                                         tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgcg                                                                      <  2:2764451/148‑1 (MQ=255)
                                                                                ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                               >  2:1342125/1‑148 (MQ=255)
                                                                                      gccgccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGcc                                                         <  2:1874653/148‑1 (MQ=255)
                                                                                           cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                    >  1:203320/1‑148 (MQ=255)
                                                                                              aCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactga                                                 >  2:2081736/1‑148 (MQ=255)
                                                                                                cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                               >  2:1534898/1‑148 (MQ=255)
                                                                                                    gtaTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCTGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACtt                                           <  2:2473636/146‑1 (MQ=255)
                                                                                                    ggATTAGTGCTATGGTTTTTGTCATCACGCTGGCATTGCAGCATAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACTGGGCTGCGCTGACTGCCCGCCTGAACTGAACACtt                                           <  1:1017758/148‑1 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          >  1:3271514/1‑148 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          >  2:3225277/1‑148 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAGTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          <  2:3098713/148‑1 (MQ=255)
                                                                                                      aTTTGTGCTATGTTTTTTGTCATCACGCTGGCATTTCAGAATAATTCCTTCGTATTTATTTACCGCCCCTTAATAATATTTTTTATCCTGATAAACGCAGCAGTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                         <  1:1515238/148‑1 (MQ=255)
                                                                                                      aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGACCACTTCt                                         >  1:348457/1‑148 (MQ=255)
                                                                                                      aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                         >  1:1237042/1‑148 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                <  2:3359633/148‑1 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                >  2:386603/1‑148 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                <  1:510454/148‑1 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                <  2:1944475/148‑1 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACGGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                <  2:1839821/148‑1 (MQ=255)
                                                                                                                aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                               >  1:2981069/1‑148 (MQ=255)
                                                                                                                aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                               <  1:2437709/148‑1 (MQ=255)
                                                                                                                 gggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                              >  2:3278208/1‑148 (MQ=255)
                                                                                                                 gggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                              >  2:3278748/1‑148 (MQ=255)
                                                                                                                 gggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                              >  1:2222566/1‑148 (MQ=255)
                                                                                                                  ggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGttt                             >  1:136624/1‑148 (MQ=255)
                                                                                                                  ggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGttt                             >  1:578485/1‑148 (MQ=255)
                                                                                                                  ggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGttt                             >  1:1870203/1‑148 (MQ=255)
                                                                                                                    ttttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                           <  1:106267/148‑1 (MQ=255)
                                                                                                                     tttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAcc                          <  1:3122420/148‑1 (MQ=255)
                                                                                                                       tGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTAAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                        <  1:1650952/148‑1 (MQ=255)
                                                                                                                         tcatcaCGCTGTCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTATACCTCCa                      >  2:739633/1‑148 (MQ=255)
                                                                                                                          catcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCAc                     >  1:981184/1‑148 (MQ=255)
                                                                                                                          catcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCAc                     >  1:2731114/1‑148 (MQ=255)
                                                                                                                            tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCTACGt                   >  2:2568328/1‑148 (MQ=255)
                                                                                                                                    gCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCCCGTCCGGACGa           >  2:1014489/1‑148 (MQ=255)
                                                                                                                                      aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATa         <  2:1057130/148‑1 (MQ=255)
                                                                                                                                      aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATa         <  1:2524476/148‑1 (MQ=255)
                                                                                                                                        tGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt       >  2:2228906/1‑148 (MQ=255)
                                                                                                                                          cagcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGc     <  1:888824/148‑1 (MQ=255)
                                                                                                                                             cagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg  >  1:3128524/1‑148 (MQ=255)
                                                                                                                                             cagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg  <  1:2286782/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG  >  CP009273/1836048‑1836336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑