Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,839,854 C→A 93.9% R455L (CGC→CTC)  topB ← DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,839,8540CA93.9% 514.8 / 20.6 213R455L (CGC→CTC) topBDNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (9/6);  new base A (83/114);  total (93/120)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGG  >  CP009273/1839709‑1839998
                                                                                                                                                 |                                                                                                                                                
gctCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCGTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCgagc                                                                                                                                                <  2:2124891/146‑1 (MQ=255)
gATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCgagc                                                                                                                                                <  2:2801991/148‑1 (MQ=255)
 aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGct                                                                                                                                               <  2:869516/148‑1 (MQ=255)
 aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGct                                                                                                                                               >  1:2013542/1‑148 (MQ=255)
  tCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGctc                                                                                                                                              >  2:3208720/1‑148 (MQ=255)
  tCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGctc                                                                                                                                              >  2:1138729/1‑148 (MQ=255)
   cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGctct                                                                                                                                             <  1:1859775/148‑1 (MQ=255)
      ggTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCCTCGCGCTCGTtg                                                                                                                                          >  2:2414314/1‑148 (MQ=255)
       gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                         <  2:1848328/148‑1 (MQ=255)
         cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgctg                                                                                                                                       >  2:484063/1‑148 (MQ=255)
         cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgctg                                                                                                                                       <  1:2455259/148‑1 (MQ=255)
           tCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGcc                                                                                                                                     <  2:1459938/148‑1 (MQ=255)
                gAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACa                                                                                                                                >  2:1041704/1‑148 (MQ=255)
                gAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACa                                                                                                                                >  2:1399561/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAg                                                                                                                               >  2:2457842/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAg                                                                                                                               >  2:2826498/1‑148 (MQ=255)
                 aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGCGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCTCTTTGGTGCCTAACac                                                                                                                               >  1:2989562/1‑147 (MQ=255)
                   aGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTGGTGCCTAACAGCg                                                                                                                             >  2:2664585/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGt                                                                                                                            >  2:1689645/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGt                                                                                                                            >  2:2053307/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCTCTTTGCTGCCTAACAGCGt                                                                                                                            >  2:2053412/1‑148 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAATCGAGCTCGTTGGGGGCGAACAGCgg                                                                                                                            >  1:2384968/1‑147 (MQ=255)
                    gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCGCGTTGCTGCCGAACAGCGt                                                                                                                            >  2:1731607/1‑148 (MQ=255)
                       gtgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCAAAGAGCTCATTGGTGGCACAAAGCGTgag                                                                                                                         >  1:1927409/1‑146 (MQ=255)
                        tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTGATCGAGCTCTTTGCTGGCGAACAGCGTgcgc                                                                                                                        >  1:1071080/1‑148 (MQ=255)
                        tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCGAAAAGCGTgcgc                                                                                                                        >  1:2735831/1‑148 (MQ=255)
                         gtTGCATCGGTAAACTGGCGCGGCGGCTGGGTGTGCCGCTCTACCACTTCACCTTGTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGcc                                                                                                                       <  2:2403200/148‑1 (MQ=255)
                         gtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGcc                                                                                                                       <  2:2403100/148‑1 (MQ=255)
                               tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                 >  2:2921416/1‑148 (MQ=255)
                               tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGGTGTTCATCGAGGTCTTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                 >  1:2532375/1‑148 (MQ=255)
                                 ggTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                               <  2:3469358/148‑1 (MQ=255)
                                 ggTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                               <  1:1843121/148‑1 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAg                                                                                                           <  1:284575/148‑1 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAAAAGCGTGCGCCAGCCTGCTTAAg                                                                                                           >  2:3295681/1‑148 (MQ=255)
                                     aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTTTTGATGGAGCTCTTTTGGGCCAAACAGCGGGCGCCCGCCCGCGTCAg                                                                                                           >  1:3267726/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCa                                                                                                         >  2:3440546/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCa                                                                                                         >  2:2596853/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCa                                                                                                         >  2:558592/1‑148 (MQ=255)
                                       aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGAGTGCCGTCGTTTTCATCAACGAGCTCTTTGCAGCCTAAAAGCGTGCGACAGCCTTCATCAGCa                                                                                                         >  2:3441901/1‑148 (MQ=255)
                                            gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                    <  1:15201/148‑1 (MQ=255)
                                             cggcggCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaaa                                                                                                   <  2:1884858/148‑1 (MQ=255)
                                             cggcggCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaaa                                                                                                   <  2:708843/148‑1 (MQ=255)
                                                 ggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgcg                                                                                               <  1:3186821/148‑1 (MQ=255)
                                                 ggcTGGGTTTGCCGCTCTACCACGTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgcg                                                                                               <  2:1160570/148‑1 (MQ=255)
                                                  gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACgcgc                                                                                              <  2:959759/148‑1 (MQ=255)
                                                    tGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCTCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGACTGCTTCAGCAAGAAAACGCGCtt                                                                                            <  1:2935716/148‑1 (MQ=255)
                                                            ccGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACa                                                                                    >  2:353423/1‑148 (MQ=255)
                                                               ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAt                                                                                 <  1:2765085/148‑1 (MQ=255)
                                                                tctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAAACGAGCTCATTTCTGCCTAAAACCGTTCGCCACCCAGCTTCAGCAACAAAAAACGCATTTTCGACaaaat                                                                                >  1:1364005/1‑146 (MQ=255)
                                                                 ctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACACCGGGCGCCAGCCAGCATCAACAAAAAAACCCGCTTTATCCACaaaaat                                                                               >  1:3456340/1‑145 (MQ=255)
                                                                   aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACCCGCGTTAGCGACAAAatttc                                                                             >  2:1626213/1‑146 (MQ=255)
                                                                   aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGCCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGc                                                                             <  2:1423643/148‑1 (MQ=255)
                                                                    ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTGATCGAGCTAATTGGTGCCGAACAGCGTGCGGCAGCCGGCGTAAGCAAGAAAACACGCTTTATCGACaaaatatac                                                                            >  1:3459856/1‑142 (MQ=255)
                                                                      aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                          <  1:762797/148‑1 (MQ=255)
                                                                      aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                          <  1:2523248/148‑1 (MQ=255)
                                                                       cTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCttt                                                                         <  1:1182603/148‑1 (MQ=255)
                                                                       cTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCttt                                                                         <  2:2341416/148‑1 (MQ=255)
                                                                        ttCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCCAGAAAACGCGCGTTAGCGACAAATTTGCCTTTg                                                                        >  2:63255/1‑148 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCTTCGCCATTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTATTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCTCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:2907459/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:3084675/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  1:1622804/148‑1 (MQ=255)
                                                                           aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCa                                                                     <  2:1066618/148‑1 (MQ=255)
                                                                            ccTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCaa                                                                    <  2:2321080/148‑1 (MQ=255)
                                                                             cTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAAt                                                                   <  1:726245/148‑1 (MQ=255)
                                                                               ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGt                                                                 <  1:1004724/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  2:2304013/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  1:676418/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTc                                                                <  1:676798/148‑1 (MQ=255)
                                                                                tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCCTTGGCAATGTc                                                                <  2:3279381/148‑1 (MQ=255)
                                                                                       caGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTTGCAATGTCCAGTTCg                                                         >  2:1953759/1‑148 (MQ=255)
                                                                                        aGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGa                                                        <  1:1101410/148‑1 (MQ=255)
                                                                                          cAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGGCTAACAGCGGGCGCCCGCCTGCGTCAGCAAGAAAACGCGCGTTAGCGGCAAATTTTCCTTTGGGAATGTCCAGTTCGAtt                                                      >  2:2685539/1‑147 (MQ=255)
                                                                                            aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTGATCGCGCTCGTTGGTGCCGAACAGCGGGCGCCCGCCGGCTTCAGCAACAAAAAGCGCGTTATCGACAAAATTTCCGTTGGGCATGTCCCCTTCGATAac                                                    >  1:1341583/1‑148 (MQ=255)
                                                                                               cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAACGCGCTTTATCGACAAAATTGCCTTTGGCAATGTCCAGTTCGTTAacaca                                                 >  1:1777497/1‑148 (MQ=255)
                                                                                               cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacaca                                                 <  1:2816796/148‑1 (MQ=255)
                                                                                                       ttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCTTTGCTGCCTAAAAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCGTTAGCGGCAAATTTGCCTTTGGCCATGTCCAGGTCGATAACACACATGCGGa                                         >  2:3020448/1‑148 (MQ=255)
                                                                                                       ttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGa                                         <  1:1238571/148‑1 (MQ=255)
                                                                                                          gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                      <  1:2155337/148‑1 (MQ=255)
                                                                                                          gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                      <  1:2155261/148‑1 (MQ=255)
                                                                                                                acaGGCAGTTGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACCGCGTGCGCCCGCCGGCTTCAGCAAGAAAACGCGCCGGAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCAt                                <  1:3350794/148‑1 (MQ=255)
                                                                                                                 caGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATc                               <  1:160208/148‑1 (MQ=255)
                                                                                                                 caGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATc                               <  1:410722/148‑1 (MQ=255)
                                                                                                                   ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                             <  1:1857608/148‑1 (MQ=255)
                                                                                                                      aGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                          <  1:3089997/148‑1 (MQ=255)
                                                                                                                           cGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAt                     <  2:118491/148‑1 (MQ=255)
                                                                                                                             tGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTg                   <  1:2535141/148‑1 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACCCCGCATCCGGGCAGAATTGCATCa              >  2:1710277/1‑148 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAATTTCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa              >  2:1709010/1‑148 (MQ=255)
                                                                                                                                  tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa              >  2:2527090/1‑148 (MQ=255)
                                                                                                                                   cgtTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGACAAATTTTCCTTTGGCAAAGTCCAGTTCGATAACACACTTGCGGAACACCCCATCCGGGCAGAATTTCATCAg             >  1:342192/1‑148 (MQ=255)
                                                                                                                                    gtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGa            <  1:1576351/148‑1 (MQ=255)
                                                                                                                                     ttttCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGAt           <  1:2334465/148‑1 (MQ=255)
                                                                                                                                           tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc     >  1:2224298/1‑148 (MQ=255)
                                                                                                                                            cATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg    <  2:2379441/148‑1 (MQ=255)
                                                                                                                                            cATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg    <  2:592879/148‑1 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   <  2:488098/148‑1 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   >  2:3003814/1‑148 (MQ=255)
                                                                                                                                             aTCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg   >  2:739455/1‑148 (MQ=255)
                                                                                                                                              tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg  <  1:952598/148‑1 (MQ=255)
                                                                                                                                              tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg  >  1:658205/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGG  >  CP009273/1839709‑1839998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑