Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,852,274 C→A 100% D273Y (GAT→TAT)  ydjJ ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,852,2740CA99.2% 749.4 / ‑3.6 229D273Y (GAT→TAT) ydjJputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (116/111);  total (118/111)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

ATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATA  >  CP009273/1852128‑1852416
                                                                                                                                                  |                                                                                                                                              
 tAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGc                                                                                                                                              >  2:24014/1‑148 (MQ=255)
 tAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGc                                                                                                                                              >  2:23259/1‑148 (MQ=255)
   aTCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCg                                                                                                                                            <  2:2753912/148‑1 (MQ=255)
       tAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTa                                                                                                                                        >  2:236446/1‑148 (MQ=255)
        aaaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTAc                                                                                                                                       >  2:626429/1‑148 (MQ=255)
           tatGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGt                                                                                                                                    <  2:3460403/148‑1 (MQ=255)
             tGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTAc                                                                                                                                  >  1:120300/1‑148 (MQ=255)
                  cACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACcaaca                                                                                                                             <  1:986710/148‑1 (MQ=255)
                  cACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACcaaca                                                                                                                             >  1:1252111/1‑148 (MQ=255)
                     cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATc                                                                                                                          >  1:2893142/1‑148 (MQ=255)
                     cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATc                                                                                                                          <  1:504297/148‑1 (MQ=255)
                     cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATc                                                                                                                          >  2:1505325/1‑148 (MQ=255)
                     cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATc                                                                                                                          >  2:1505509/1‑148 (MQ=255)
                       tCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCAt                                                                                                                        >  1:705324/1‑148 (MQ=255)
                       tCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCAt                                                                                                                        >  1:1025860/1‑148 (MQ=255)
                        cGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATa                                                                                                                       >  2:3075368/1‑148 (MQ=255)
                           tttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAtt                                                                                                                    >  2:1727355/1‑148 (MQ=255)
                            ttCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTCCAGTACCAACAATCATAAttt                                                                                                                   >  2:156072/1‑148 (MQ=255)
                                  tCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTAccgc                                                                                                             <  1:3401236/148‑1 (MQ=255)
                                     aaTCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACcgccgc                                                                                                          >  2:2935503/1‑148 (MQ=255)
                                      aTCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCcgcg                                                                                                         >  2:2207066/1‑148 (MQ=255)
                                      aTCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCcgcg                                                                                                         <  2:596268/148‑1 (MQ=255)
                                      aTCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCcgcg                                                                                                         <  2:1003324/148‑1 (MQ=255)
                                       tCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCgcgc                                                                                                        >  2:965960/1‑148 (MQ=255)
                                       tCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCgcgc                                                                                                        >  1:3283906/1‑148 (MQ=255)
                                       tCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCgcgc                                                                                                        >  2:2719416/1‑148 (MQ=255)
                                        cGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCa                                                                                                       <  2:2651236/148‑1 (MQ=255)
                                           ccTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTa                                                                                                    <  2:2011957/148‑1 (MQ=255)
                                             tGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTAcc                                                                                                  >  1:349886/1‑148 (MQ=255)
                                              gaagaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCa                                                                                                 >  2:3326152/1‑148 (MQ=255)
                                               aagaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAg                                                                                                <  1:2613587/148‑1 (MQ=255)
                                               aagaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAg                                                                                                <  1:2814883/148‑1 (MQ=255)
                                                agaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGa                                                                                               <  2:562456/148‑1 (MQ=255)
                                                 gaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAt                                                                                              >  1:2765214/1‑148 (MQ=255)
                                                  aaaTAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATa                                                                                             <  1:3352094/148‑1 (MQ=255)
                                                      aGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGt                                                                                         >  1:3102665/1‑148 (MQ=255)
                                                        cTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGc                                                                                       >  2:125776/1‑148 (MQ=255)
                                                           cAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTg                                                                                    <  2:1374553/148‑1 (MQ=255)
                                                           cAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTg                                                                                    <  2:1311143/148‑1 (MQ=255)
                                                             aTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACAAGATAAGGTGCCTGtt                                                                                  >  2:192357/1‑148 (MQ=255)
                                                              tCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGttt                                                                                 <  2:1093320/148‑1 (MQ=255)
                                                              tCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGttt                                                                                 >  2:1083074/1‑148 (MQ=255)
                                                               cGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                <  2:1753540/148‑1 (MQ=255)
                                                                        aTAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAcc                                                                       >  2:1448634/1‑148 (MQ=255)
                                                                         tAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCg                                                                      >  1:2658975/1‑148 (MQ=255)
                                                                         tAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCg                                                                      <  2:3096305/148‑1 (MQ=255)
                                                                            cGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAg                                                                   <  1:664216/148‑1 (MQ=255)
                                                                            cGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAg                                                                   <  1:2425954/148‑1 (MQ=255)
                                                                              aTTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                 >  2:910345/1‑148 (MQ=255)
                                                                               tttgCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGTGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAc                                                                <  1:459647/145‑1 (MQ=255)
                                                                                tGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAcc                                                               <  1:191096/148‑1 (MQ=255)
                                                                                  gCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCg                                                             >  1:2554221/1‑148 (MQ=255)
                                                                                  gCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCg                                                             <  2:2954619/148‑1 (MQ=255)
                                                                                   cATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGc                                                            <  1:2951313/148‑1 (MQ=255)
                                                                                     tAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTg                                                          <  1:2157886/148‑1 (MQ=255)
                                                                                     tAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTg                                                          <  2:198072/148‑1 (MQ=255)
                                                                                      aGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGc                                                            >  2:1208224/1‑145 (MQ=255)
                                                                                      aGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGt                                                         <  2:2942215/148‑1 (MQ=255)
                                                                                        cGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGttt                                                       >  1:2015288/1‑148 (MQ=255)
                                                                                          gAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCg                                                     >  1:748163/1‑148 (MQ=255)
                                                                                          gAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCg                                                     >  1:3035093/1‑148 (MQ=255)
                                                                                            aTACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACACGCTGTTTCGaa                                                   >  2:1849968/1‑148 (MQ=255)
                                                                                             tACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGaaa                                                  <  2:3245224/148‑1 (MQ=255)
                                                                                              aCCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGaaaa                                                 >  2:1395076/1‑148 (MQ=255)
                                                                                              aCCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAATTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGaaaa                                                 <  2:1025860/148‑1 (MQ=255)
                                                                                               ccGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGACAAc                                                >  1:3030023/1‑148 (MQ=255)
                                                                                                   cTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAata                                            >  1:1691677/1‑148 (MQ=255)
                                                                                                   cTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAata                                            >  1:2007993/1‑148 (MQ=255)
                                                                                                   cTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTGTCGAAAACAata                                            >  1:1691513/1‑148 (MQ=255)
                                                                                                     ggATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATc                                          >  1:2483304/1‑148 (MQ=255)
                                                                                                      gATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCt                                         >  2:1188411/1‑148 (MQ=255)
                                                                                                       aTAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTg                                        <  2:18350/148‑1 (MQ=255)
                                                                                                         aGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTgcg                                      >  1:1502035/1‑148 (MQ=255)
                                                                                                           ttaCTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGcc                                    >  1:2694319/3‑148 (MQ=255)
                                                                                                           tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGcc                                    >  2:2623907/1‑148 (MQ=255)
                                                                                                             aCTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCa                                  >  2:838297/1‑148 (MQ=255)
                                                                                                                tCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGt                               <  1:1083074/148‑1 (MQ=255)
                                                                                                                   cgATTGATTTTTAGGAAATTGATTGCCGAATAGCCGTGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCtt                            <  2:2113202/148‑1 (MQ=255)
                                                                                                                   cgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCtt                            >  1:3185377/1‑148 (MQ=255)
                                                                                                                   cgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCtt                            >  1:2366342/1‑148 (MQ=255)
                                                                                                                   cgATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCtt                            >  1:2366549/1‑148 (MQ=255)
                                                                                                                    gattgattTTGAGGAAATTGATTGTCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTc                           <  1:2174230/148‑1 (MQ=255)
                                                                                                                     attgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                          <  1:1505509/148‑1 (MQ=255)
                                                                                                                     attgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                          <  1:1505325/148‑1 (MQ=255)
                                                                                                                     attgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                          >  1:1115986/1‑148 (MQ=255)
                                                                                                                      ttgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCgg                         >  2:374279/1‑148 (MQ=255)
                                                                                                                         attTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaa                      >  2:3508645/1‑148 (MQ=255)
                                                                                                                          ttttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaaa                     <  2:1451452/148‑1 (MQ=255)
                                                                                                                          ttttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaaa                     <  1:1953408/148‑1 (MQ=255)
                                                                                                                          ttttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaaa                     <  1:1003446/148‑1 (MQ=255)
                                                                                                                           tttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAAt                    <  1:3434120/148‑1 (MQ=255)
                                                                                                                                ggAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTg               <  1:910345/148‑1 (MQ=255)
                                                                                                                                ggAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTg               >  1:1454304/1‑148 (MQ=255)
                                                                                                                                    attgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAg           >  1:1361467/1‑148 (MQ=255)
                                                                                                                                    attgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAg           >  1:828332/1‑148 (MQ=255)
                                                                                                                                      tgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCg         >  1:2261264/1‑148 (MQ=255)
                                                                                                                                      tgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCg         <  1:1634465/148‑1 (MQ=255)
                                                                                                                                          tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCa     >  2:1065744/1‑148 (MQ=255)
                                                                                                                                            ccGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAAt   <  2:16188/148‑1 (MQ=255)
                                                                                                                                             cGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATa  <  2:1491161/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
ATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATA  >  CP009273/1852128‑1852416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑