Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,885,611 G→T 100% R628S (CGT→AGT)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,885,6110GT98.6% 653.7 / 0.5 211R628S (CGT→AGT) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (133/75);  total (135/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

TCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCC  >  CP009273/1885466‑1885755
                                                                                                                                                 |                                                                                                                                                
 cgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                               >  2:2767681/1‑148 (MQ=255)
   ttCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCg                                                                                                                                             >  1:2402449/1‑148 (MQ=255)
   ttCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCg                                                                                                                                             >  1:1200761/1‑148 (MQ=255)
    tcccccgggCGACAGAGCCGGCCTCTGTCGCGGTATCGATAGCCAGAAGTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTAGCGCAAGGAAACTAACCGc                                                                                                                                            <  2:51572/142‑1 (MQ=255)
     ccACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCa                                                                                                                                           >  2:2031520/1‑148 (MQ=255)
     ccACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCa                                                                                                                                           <  1:2005192/148‑1 (MQ=255)
     ccACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCa                                                                                                                                           <  2:3352066/148‑1 (MQ=255)
     ccACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCa                                                                                                                                           <  1:1090678/148‑1 (MQ=255)
           ggCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCa                                                                                                                                     >  1:424214/1‑148 (MQ=255)
           ggCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCa                                                                                                                                     >  2:2819402/1‑148 (MQ=255)
           ggCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAAGTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCa                                                                                                                                     <  1:1247794/148‑1 (MQ=255)
            gCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCaa                                                                                                                                    <  2:1448112/148‑1 (MQ=255)
               aCAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATGACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGt                                                                                                                                 <  2:1353312/148‑1 (MQ=255)
                cAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGtt                                                                                                                                >  1:526007/1‑147 (MQ=255)
                cAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATAGCAAGGAAACTAACCGCACGGGCAATGTc                                                                                                                                >  1:853022/1‑148 (MQ=255)
                   agCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACg                                                                                                                             <  1:2585097/148‑1 (MQ=255)
                     cAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACggg                                                                                                                           >  2:2477817/1‑148 (MQ=255)
                      aGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGt                                                                                                                          >  1:2077114/1‑148 (MQ=255)
                      aGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGt                                                                                                                          >  1:2211678/1‑148 (MQ=255)
                       ggCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTg                                                                                                                         >  1:3471116/1‑148 (MQ=255)
                        gCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGc                                                                                                                        >  1:2523061/1‑148 (MQ=255)
                         ccTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCg                                                                                                                       <  2:829988/148‑1 (MQ=255)
                           tctGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTg                                                                                                                     >  1:121622/1‑148 (MQ=255)
                              gTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGgcg                                                                                                                  >  2:2319860/1‑148 (MQ=255)
                              gTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGgcg                                                                                                                  >  1:2336523/1‑148 (MQ=255)
                                cgcgGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGcgcg                                                                                                                <  2:2164049/148‑1 (MQ=255)
                                  cgGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcggg                                                                                                              <  1:1112545/148‑1 (MQ=255)
                                    gTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcgggcg                                                                                                            >  2:706842/1‑148 (MQ=255)
                                      aTCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGggcggc                                                                                                          >  2:1702379/1‑148 (MQ=255)
                                        cGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                        >  1:1108561/1‑148 (MQ=255)
                                        cGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                        >  2:1096537/1‑148 (MQ=255)
                                        cGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACAATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                        >  2:2261993/1‑148 (MQ=255)
                                           tAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACt                                                                                                     <  1:3114038/148‑1 (MQ=255)
                                           tAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACt                                                                                                     >  1:651025/1‑148 (MQ=255)
                                               cAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCAGACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCg                                                                                                 <  1:95119/148‑1 (MQ=255)
                                                 gAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCgag                                                                                               >  1:1546332/1‑148 (MQ=255)
                                                  aaTTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCCAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCgaga                                                                                              >  2:3158515/1‑148 (MQ=255)
                                                   aTTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaa                                                                                             >  1:231828/1‑148 (MQ=255)
                                                   aTTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaa                                                                                             >  1:878444/1‑148 (MQ=255)
                                                    ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGc                                                                                                  <  2:2520829/142‑1 (MQ=255)
                                                    ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaaa                                                                                            >  2:1154192/1‑148 (MQ=255)
                                                    ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaaa                                                                                            >  2:2291559/1‑148 (MQ=255)
                                                    ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaaa                                                                                            >  2:2291505/1‑148 (MQ=255)
                                                     tCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAAc                                                                                           >  1:2289849/1‑148 (MQ=255)
                                                        cATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTc                                                                                        >  1:185470/1‑148 (MQ=255)
                                                         aTGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcg                                                                                       <  1:1735559/148‑1 (MQ=255)
                                                           gggTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgcg                                                                                     >  1:2520679/1‑148 (MQ=255)
                                                           gggTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgcg                                                                                     >  1:761524/1‑148 (MQ=255)
                                                            ggTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCgcgc                                                                                    >  1:484498/1‑148 (MQ=255)
                                                            ggTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCgcgc                                                                                    >  2:674916/1‑148 (MQ=255)
                                                             gTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGcc                                                                                   >  1:8057/1‑148 (MQ=255)
                                                               cTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGt                                                                                 >  2:767317/1‑148 (MQ=255)
                                                                    tGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGa                                                                            >  2:434164/1‑148 (MQ=255)
                                                                      cTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACg                                                                          >  1:393781/1‑148 (MQ=255)
                                                                       ttAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGc                                                                         >  2:3122493/1‑148 (MQ=255)
                                                                           aTCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCa                                                                     <  1:1017031/148‑1 (MQ=255)
                                                                            tCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCAc                                                                    <  1:1321720/148‑1 (MQ=255)
                                                                             cAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCAcc                                                                   <  2:2448253/148‑1 (MQ=255)
                                                                             cAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCAcc                                                                   >  2:1965604/1‑148 (MQ=255)
                                                                                tAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGc                                                                <  2:853022/148‑1 (MQ=255)
                                                                                 aaaaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGcc                                                               >  1:2463251/1‑148 (MQ=255)
                                                                                  caaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCg                                                              <  2:2088666/147‑1 (MQ=255)
                                                                                  aaaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCg                                                              <  2:1200170/148‑1 (MQ=255)
                                                                                    aaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAt                                                            <  1:1702379/148‑1 (MQ=255)
                                                                                     aGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAtt                                                           >  2:1759623/1‑148 (MQ=255)
                                                                                     aGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAtt                                                           >  2:1760188/1‑148 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                                 >  1:1600865/1‑148 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                                 <  2:418109/148‑1 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                                 <  2:3027225/148‑1 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                              >  1:3351413/1‑148 (MQ=255)
                                                                                                    catacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAg                                            >  1:1542356/1‑148 (MQ=255)
                                                                                                     atacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGc                                           >  2:731001/1‑148 (MQ=255)
                                                                                                      tacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCa                                          >  1:2232435/1‑148 (MQ=255)
                                                                                                      tacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCa                                          >  2:1980235/1‑148 (MQ=255)
                                                                                                          ccGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGcc                                      >  1:1934447/1‑148 (MQ=255)
                                                                                                           cGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTTACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCa                                     <  2:525975/148‑1 (MQ=255)
                                                                                                             tAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACg                                   <  2:1651246/148‑1 (MQ=255)
                                                                                                              aaCAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGa                                  <  2:1447348/148‑1 (MQ=255)
                                                                                                              aaCAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGa                                  >  2:1380066/1‑148 (MQ=255)
                                                                                                                cAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtc                                >  2:822381/1‑148 (MQ=255)
                                                                                                                 aaaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcg                               <  2:3378982/148‑1 (MQ=255)
                                                                                                                 aaaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcg                               >  2:378566/1‑148 (MQ=255)
                                                                                                                  aaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgt                              <  1:2764913/148‑1 (MQ=255)
                                                                                                                  aaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgt                              >  1:1507952/1‑148 (MQ=255)
                                                                                                                   aTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgtc                             >  1:2976989/1‑148 (MQ=255)
                                                                                                                   aTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgtc                             >  2:3315239/1‑148 (MQ=255)
                                                                                                                       ccTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCg                         <  2:3285759/148‑1 (MQ=255)
                                                                                                                           gaggaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTcgc                     >  1:2493242/1‑148 (MQ=255)
                                                                                                                              gaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGaa                  <  2:797299/148‑1 (MQ=255)
                                                                                                                              gaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGaa                  >  1:842634/1‑148 (MQ=255)
                                                                                                                               aTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAAt                 <  1:3288304/148‑1 (MQ=255)
                                                                                                                                tGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATc                >  1:2380171/1‑148 (MQ=255)
                                                                                                                                tGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATc                >  1:1038543/1‑148 (MQ=255)
                                                                                                                                   tATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGt             >  1:3068397/1‑148 (MQ=255)
                                                                                                                                     tCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCg           <  2:1511894/148‑1 (MQ=255)
                                                                                                                                        cAAGGAAACTCACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGccc        <  1:2385602/148‑1 (MQ=255)
                                                                                                                                          aGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTa      >  2:692535/1‑148 (MQ=255)
                                                                                                                                          aGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTa      >  1:1795772/1‑148 (MQ=255)
                                                                                                                                             aaaCTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTAccc   <  2:3168960/148‑1 (MQ=255)
                                                                                                                                             aaaCTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTAccc   >  1:3242443/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCC  >  CP009273/1885466‑1885755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑