| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,932,296 | G→T | 100% | D131Y (GAT→TAT) | pykA → | pyruvate kinase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,932,296 | 0 | G | T | 96.1% | 541.3 / 10.5 | 179 | D131Y (GAT→TAT) | pykA | pyruvate kinase II |
| Reads supporting (aligned to +/- strand): ref base G (6/1); new base T (87/85); total (93/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 179 total aligned reads displayed.
AGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGAAAAAGACA > CP009273/1932151‑1932440 | agaagGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGAGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTAt < 2:801022/148‑1 (MQ=255) aagGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATgg < 2:1858992/148‑1 (MQ=255) aagGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATgg < 2:1263040/148‑1 (MQ=255) aaaGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTcgcg > 1:32459/1‑148 (MQ=255) ttttCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCa < 2:1534936/148‑1 (MQ=255) ttttCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCa < 2:1765251/148‑1 (MQ=255) ccTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTa > 2:455349/1‑148 (MQ=255) ccTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTa > 2:1313326/1‑148 (MQ=255) cTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTaa > 2:2067183/1‑148 (MQ=255) cTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTaa < 1:1495290/148‑1 (MQ=255) cAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTaaaa > 1:2054686/1‑148 (MQ=255) cAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTaaaa < 1:3157388/148‑1 (MQ=255) cAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTaaaa > 1:766795/1‑148 (MQ=255) cAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTcaaa > 1:3132887/1‑148 (MQ=255) atatTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGt < 1:2133521/148‑1 (MQ=255) ttGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACt > 1:143915/1‑148 (MQ=255) tGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTg > 1:1733985/1‑148 (MQ=255) tGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTg > 1:2567561/1‑148 (MQ=255) tGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTg > 1:2392690/1‑148 (MQ=255) gggATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGa > 2:2166049/1‑148 (MQ=255) gATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAg > 1:2886001/1‑148 (MQ=255) gATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAg < 1:1238789/148‑1 (MQ=255) gATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAg < 2:1114134/148‑1 (MQ=255) aTAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCTTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGt < 2:40416/148‑1 (MQ=255) aTAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGt > 2:1085325/1‑148 (MQ=255) aTAAAGTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGt < 1:624124/148‑1 (MQ=255) aaaTTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTc < 1:2640167/148‑1 (MQ=255) ttCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAgg > 2:278804/1‑148 (MQ=255) cTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACACCGAAACAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGATAAAAGTACTGGAAGTTCAGGGCa < 2:1841730/148‑1 (MQ=255) cTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGa > 1:2692612/1‑148 (MQ=255) tCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGaa < 1:1110215/148‑1 (MQ=255) gACGCCAACCTGTGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAg < 1:2729032/148‑1 (MQ=255) aCGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAgt > 1:398731/1‑148 (MQ=255) cGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCTGGGCATGAAAgtg < 2:2095193/148‑1 (MQ=255) gCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAgtgt < 2:2097304/148‑1 (MQ=255) cAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTc < 2:766795/148‑1 (MQ=255) cAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTc < 2:3015259/148‑1 (MQ=255) aCCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCAc > 1:499426/1‑148 (MQ=255) ccTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCAcc > 2:1423692/1‑148 (MQ=255) tGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGa > 2:2457554/1‑148 (MQ=255) ggTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAg < 1:1852206/148‑1 (MQ=255) ggTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAg < 1:3403683/148‑1 (MQ=255) ggTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAg > 1:1308577/1‑148 (MQ=255) gTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGt < 1:3329699/148‑1 (MQ=255) aaaGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCa < 2:2981644/148‑1 (MQ=255) aaGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCAc > 1:875039/1‑148 (MQ=255) tGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGACGTCACCGTc > 1:2129165/1‑148 (MQ=255) tGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTc > 2:2608867/1‑148 (MQ=255) ggCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCggtg > 1:1401879/1‑148 (MQ=255) gACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTc < 1:2136412/148‑1 (MQ=255) cAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTccc < 1:1090950/148‑1 (MQ=255) aaGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCct < 2:1204844/148‑1 (MQ=255) gAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCctct < 1:430736/148‑1 (MQ=255) aaaaaGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCGGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCtctc > 2:1628430/1‑148 (MQ=255) aaaaaGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCtctc < 1:1250792/148‑1 (MQ=255) aaaaaGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCtctc > 1:2580696/1‑148 (MQ=255) aaaaaGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCtctc > 1:2580872/1‑148 (MQ=255) aaaaaGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCtctc > 1:2666823/1‑148 (MQ=255) gTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCcaaca < 2:1385636/148‑1 (MQ=255) cGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAAt > 1:1470804/1‑148 (MQ=255) tATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATaaa > 2:1382012/1‑148 (MQ=255) aTCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAg < 2:928329/148‑1 (MQ=255) aTCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAg > 1:484516/1‑148 (MQ=255) aTCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAg > 1:2240209/1‑148 (MQ=255) tCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAgg < 2:2908045/148‑1 (MQ=255) cGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGt < 1:1951337/148‑1 (MQ=255) cGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGt < 2:3278327/148‑1 (MQ=255) aCTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTAt > 1:2123005/1‑148 (MQ=255) aCTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTAt < 2:1175687/148‑1 (MQ=255) aCAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATcaa > 1:2535174/1‑148 (MQ=255) aaGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATcaacaa > 2:740116/1‑148 (MQ=255) aaGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATcaacaa < 1:2067183/148‑1 (MQ=255) aGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACaaa > 1:2593432/1‑148 (MQ=255) aGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACaaa > 1:1022065/1‑148 (MQ=255) ggCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAAc > 1:1551811/1‑148 (MQ=255) gcctgcctGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACt > 2:638419/1‑148 (MQ=255) gcctgcctGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACt > 2:2085909/1‑148 (MQ=255) tgcctgcTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTgg > 1:3009161/1‑148 (MQ=255) tgcctgcTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTgg < 1:1144873/148‑1 (MQ=255) tgcctgcTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTgg < 1:1926868/148‑1 (MQ=255) ctgctgACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTggcgg > 2:2358912/1‑148 (MQ=255) tgACGTCGTGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGt < 2:1202272/148‑1 (MQ=255) tcgtGCCTGGTGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTc > 2:556124/1‑148 (MQ=255) gTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAgc > 2:3226452/1‑148 (MQ=255) tGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAgcg < 1:3041427/148‑1 (MQ=255) tGACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAgcg > 2:2732587/1‑148 (MQ=255) gACATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAgcgc < 1:2406129/148‑1 (MQ=255) aCATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCt > 2:2350767/1‑148 (MQ=255) cATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTg > 2:2589335/1‑148 (MQ=255) cATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTg < 2:517634/148‑1 (MQ=255) cATCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTg < 2:517927/148‑1 (MQ=255) cATCCTGCTGCAGGACTATGGTCTCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTg < 2:3386297/148‑1 (MQ=255) aTCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGa < 1:2931798/148‑1 (MQ=255) tCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGAc > 1:2947242/1‑148 (MQ=255) tCCTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGAc > 1:2947237/1‑148 (MQ=255) ccTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGAcc > 1:2839753/1‑148 (MQ=255) ccTGCTGCTGGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGAcc > 1:1232763/1‑148 (MQ=255) tgctgctgGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGa < 2:1990902/148‑1 (MQ=255) tgctgGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGaaaa < 2:2567561/148‑1 (MQ=255) ctgGACTATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGAAAAAg < 2:1838107/148‑1 (MQ=255) | AGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTCGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTAAAAGTACTGGAAGTTCAGGGCATGAAAGTGTTCACCGAAGTCACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGAAAAAGACA > CP009273/1932151‑1932440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |