Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,962,923 C→A 100% M233I (ATG→ATT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,962,9230CA100.0% 553.5 / ‑6.7 169M233I (ATG→ATT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (73/94);  minor base T (0/2);  total (73/96)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

ATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAA  >  CP009273/1962779‑1963070
                                                                                                                                                |                                                                                                                                                   
  acacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGAc                                                                                                                                                >  2:2830928/1‑148 (MQ=255)
   cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                               >  2:2906364/1‑148 (MQ=255)
     ccGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGtt                                                                                                                                             >  1:2051680/1‑148 (MQ=255)
      cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                            <  2:869663/148‑1 (MQ=255)
      cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                            >  2:850733/1‑148 (MQ=255)
      cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                            <  1:1451008/148‑1 (MQ=255)
         cTGACCACGCATCTTGAAGCGTCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACAAGCAAACAGCAATCCGTCGGGATTAATGAGCGGAACAAAGCGTCGCAAAATCTCCTGCTGTGTAGATTGATCGAAGTATATAATGACGTTACGa                                                                                                                                         >  2:1111893/1‑148 (MQ=255)
         cTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTATATAATGACGTTACGa                                                                                                                                         >  1:2235011/1‑148 (MQ=255)
           gACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACa                                                                                                                                       >  1:251959/1‑148 (MQ=255)
            aCCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACag                                                                                                                                      >  2:2173445/1‑148 (MQ=255)
            aCCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACag                                                                                                                                      >  2:643035/1‑148 (MQ=255)
             ccACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                     <  1:725765/148‑1 (MQ=255)
             acACGCAGCGTTATGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                     <  1:658511/147‑1 (MQ=255)
               aCGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaag                                                                                                                                   >  2:1851621/1‑148 (MQ=255)
                cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                  <  2:3006373/148‑1 (MQ=255)
                cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                  >  1:2013579/1‑148 (MQ=255)
                         aaGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaa                                                                                                                         <  2:1086794/148‑1 (MQ=255)
                               cgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGAAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGAGGTTACGACAGAAGATCGCATCAAACGGcc                                                                                                                   <  1:1755266/148‑1 (MQ=255)
                                     aGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGAAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCa                                                                                                             <  1:838177/148‑1 (MQ=255)
                                       gTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                           >  2:2133801/1‑148 (MQ=255)
                                       gTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                           <  1:3076355/148‑1 (MQ=255)
                                       gTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                           <  1:2556387/148‑1 (MQ=255)
                                       gTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                           <  1:1117882/148‑1 (MQ=255)
                                           cTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtgt                                                                                                       >  1:1743931/1‑148 (MQ=255)
                                            tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTa                                                                                                      >  1:2010870/1‑148 (MQ=255)
                                             aaaTTTTTCAGAGTGCCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGCAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                     <  1:3007669/148‑1 (MQ=255)
                                              aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                    >  1:277277/1‑148 (MQ=255)
                                              aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGAAAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                    >  1:1289411/1‑148 (MQ=255)
                                               aGTTTTCAGAGTCACCCGCAAACAGCACTCCGTCGGGTTTAAGGAGCGCAACAAAGCGGCGCAAAATCTCCTGCTGGTTTGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGACCGCATCAAACGGCCCCGGCACGGTGTACTg                                                                                                   <  1:2938387/148‑1 (MQ=255)
                                                     cAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCGGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGc                                                                                             <  2:885296/148‑1 (MQ=255)
                                                        agTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAg                                                                                          >  2:1238892/1‑148 (MQ=255)
                                                            aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGa                                                                                      <  1:3188673/148‑1 (MQ=255)
                                                             cccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                     >  1:123189/1‑148 (MQ=255)
                                                              ccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACTGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAtt                                                                                    >  1:2808301/1‑148 (MQ=255)
                                                               cGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                   <  2:1973821/148‑1 (MQ=255)
                                                               cGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                   <  2:2213957/148‑1 (MQ=255)
                                                                    aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                              <  2:1812366/148‑1 (MQ=255)
                                                                    aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                              >  2:2731038/1‑148 (MQ=255)
                                                                    aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                              >  2:2664019/1‑148 (MQ=255)
                                                                    aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                              >  2:3281258/1‑148 (MQ=255)
                                                                     cAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCggg                                                                             >  1:87838/1‑148 (MQ=255)
                                                                       gCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGc                                                                           >  2:1493178/1‑148 (MQ=255)
                                                                          aTCCGTCGGGTTTAAGGAGCGGAACACCGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGACGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                        <  2:3196767/148‑1 (MQ=255)
                                                                           tCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                       >  1:141839/1‑148 (MQ=255)
                                                                            ccGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAAt                                                                      <  2:1457022/148‑1 (MQ=255)
                                                                               tCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCaa                                                                   <  1:975244/148‑1 (MQ=255)
                                                                               tCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCaa                                                                   <  2:2241861/148‑1 (MQ=255)
                                                                               tCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCaa                                                                   <  1:2731038/148‑1 (MQ=255)
                                                                                cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                                  <  1:709955/148‑1 (MQ=255)
                                                                                cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                                  <  1:2173445/148‑1 (MQ=255)
                                                                                   gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                               >  1:1579851/1‑148 (MQ=255)
                                                                                   gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                               >  2:2663263/1‑148 (MQ=255)
                                                                                   gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                               >  2:526973/1‑148 (MQ=255)
                                                                                    tttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAg                                                              >  1:1334184/1‑148 (MQ=255)
                                                                                     ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                             >  2:972006/1‑148 (MQ=255)
                                                                                     ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                             >  2:2726974/1‑148 (MQ=255)
                                                                                     ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                             <  2:797256/148‑1 (MQ=255)
                                                                                        aGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                          >  1:1041951/1‑148 (MQ=255)
                                                                                        aGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                          >  1:2073640/1‑148 (MQ=255)
                                                                                        aGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                          >  1:2073643/1‑148 (MQ=255)
                                                                                         ggAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                         <  1:57088/148‑1 (MQ=255)
                                                                                          gAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                        >  2:665595/1‑148 (MQ=255)
                                                                                          gAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                        >  1:2687187/1‑148 (MQ=255)
                                                                                            gCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAg                                                      <  2:2164292/148‑1 (MQ=255)
                                                                                              ggAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCt                                                    <  1:2735182/148‑1 (MQ=255)
                                                                                               gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTc                                                   >  1:1846436/1‑148 (MQ=255)
                                                                                                aacaccgcggcgCAATATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCACACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                                  <  2:1289411/142‑1 (MQ=255)
                                                                                                aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                                  <  2:2814983/148‑1 (MQ=255)
                                                                                                  cacaGCGTCGCAAAATCTCGTGCTGGGTTGTTTGATCGAAGTAGATTTTGACGTTACGACTGAAGATCGCATCAAACGGCCCCGGCACTTTGTAATGTTTCGCCAGTAGTTTCAGCGGGGATATATGTACATAGTTCGGCATCTCCTg                                                <  2:2319969/145‑1 (MQ=255)
                                                                                                  cAAAGCGGCGCAAAATCTCCTGCTGGGTATTTTTATCGAAGTAGTTAATGACGTTACGACTGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTg                                                <  2:1140973/148‑1 (MQ=255)
                                                                                                  cAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTg                                                <  2:556550/148‑1 (MQ=255)
                                                                                                  aaatgcggcgCAAAATCTCCTGATGGGTATTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCTGTAGATTGAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTg                                                <  2:2319842/144‑1 (MQ=255)
                                                                                                     aGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCTCCAGCTCCTGacg                                             >  1:1908749/1‑148 (MQ=255)
                                                                                                     aGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacg                                             >  2:3426842/1‑148 (MQ=255)
                                                                                                     aGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacg                                             >  2:3426887/1‑148 (MQ=255)
                                                                                                       cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                           <  1:173742/148‑1 (MQ=255)
                                                                                                       cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                           <  1:173764/148‑1 (MQ=255)
                                                                                                            cAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGt                                      <  1:3422547/148‑1 (MQ=255)
                                                                                                              aaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAc                                    <  2:3084701/148‑1 (MQ=255)
                                                                                                               aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                   >  1:575477/1‑148 (MQ=255)
                                                                                                               aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                   <  2:1412937/148‑1 (MQ=255)
                                                                                                                        ctgGGTTGTTTGATCGAAGTAGATATTGAGGTTACGACAGATGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGAAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGACCTTCa                          <  1:3487243/148‑1 (MQ=255)
                                                                                                                        ctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCTCTTCa                          <  2:2392962/148‑1 (MQ=255)
                                                                                                                        ctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCTCTTCa                          <  2:631754/148‑1 (MQ=255)
                                                                                                                        ctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCa                          <  1:3488420/148‑1 (MQ=255)
                                                                                                                          gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATg                        <  1:2041684/148‑1 (MQ=255)
                                                                                                                          gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATg                        >  1:407827/1‑148 (MQ=255)
                                                                                                                           ggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTNTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                       >  1:1779755/1‑148 (MQ=255)
                                                                                                                              aGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGc                    <  2:621604/148‑1 (MQ=255)
                                                                                                                              aGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGc                    <  2:2375034/148‑1 (MQ=255)
                                                                                                                               gTTTGATCGAAGTAGATAATGACGTTACGACAGAAGTTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcc                   <  1:502577/148‑1 (MQ=255)
                                                                                                                                   gATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGt               <  2:1692809/148‑1 (MQ=255)
                                                                                                                                    aTCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTc              <  2:2042229/148‑1 (MQ=255)
                                                                                                                                     tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCTGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc             <  2:1201545/148‑1 (MQ=255)
                                                                                                                                      cGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTccc            <  1:2830928/148‑1 (MQ=255)
                                                                                                                                         aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg         >  1:1152763/1‑148 (MQ=255)
                                                                                                                                          gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc        <  2:2687187/148‑1 (MQ=255)
                                                                                                                                          gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc        <  1:2375203/148‑1 (MQ=255)
                                                                                                                                           tAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCa       <  1:1431665/148‑1 (MQ=255)
                                                                                                                                                cATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGaaa  >  2:1428488/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAA  >  CP009273/1962779‑1963070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑