Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,963,075 G→T 93.9% R183R (CGG→AGG)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,963,0750GT93.9% 542.9 / 27.3 196R183R (CGG→AGG) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (7/5);  new base T (95/89);  total (102/94)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 200 total aligned reads displayed.

TTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATGTGTCAGCCAGCGTCAT  >  CP009273/1962930‑1963219
                                                                                                                                                 |                                                                                                                                                
ttACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCttt                                                                                                                                                >  2:2362222/1‑148 (MQ=255)
ttACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCttt                                                                                                                                                >  2:2362364/1‑148 (MQ=255)
     aCAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGt                                                                                                                                           >  2:205319/1‑148 (MQ=255)
      cAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGtt                                                                                                                                          <  1:621955/148‑1 (MQ=255)
         aagaTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTtgct                                                                                                                                       >  1:3302008/1‑148 (MQ=255)
          agaTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTtgctg                                                                                                                                      <  2:1536524/148‑1 (MQ=255)
            aTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTgcg                                                                                                                                    >  1:1199672/1‑148 (MQ=255)
            aTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTgcg                                                                                                                                    >  1:2779213/1‑148 (MQ=255)
            aTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTgcg                                                                                                                                    <  2:3023763/148‑1 (MQ=255)
             tCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTgcgg                                                                                                                                   <  2:645578/148‑1 (MQ=255)
             tCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTgcgg                                                                                                                                   >  2:2670583/1‑148 (MQ=255)
                cATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGt                                                                                                                                <  1:2726974/148‑1 (MQ=255)
                 aTCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTc                                                                                                                               >  1:752955/1‑148 (MQ=255)
                    aaaCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAgg                                                                                                                            >  1:1091623/1‑148 (MQ=255)
                     aaCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGt                                                                                                                           <  1:1127199/148‑1 (MQ=255)
                      aCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGtt                                                                                                                          <  2:141839/148‑1 (MQ=255)
                      aCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGtt                                                                                                                          <  2:766930/148‑1 (MQ=255)
                      aCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGtt                                                                                                                          >  1:1937536/1‑148 (MQ=255)
                           cccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCa                                                                                                                     <  2:1715936/148‑1 (MQ=255)
                              ggCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAAct                                                                                                                  <  2:407827/148‑1 (MQ=255)
                                cACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAActct                                                                                                                >  2:190868/1‑148 (MQ=255)
                                 aCGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCtt                                                                                                               <  1:2253168/148‑1 (MQ=255)
                                  cGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTc                                                                                                              <  1:1493178/148‑1 (MQ=255)
                                      gtACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATgg                                                                                                          <  1:2133801/148‑1 (MQ=255)
                                       tACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGc                                                                                                         <  1:526973/148‑1 (MQ=255)
                                       tACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGc                                                                                                         >  2:3052695/1‑148 (MQ=255)
                                        aCTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCg                                                                                                        <  2:277277/148‑1 (MQ=255)
                                         cTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCga                                                                                                       <  1:872658/148‑1 (MQ=255)
                                           gTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgata                                                                                                     >  1:964126/1‑148 (MQ=255)
                                           gTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgata                                                                                                     >  1:2291716/1‑148 (MQ=255)
                                            tttCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatag                                                                                                    >  1:3151803/1‑148 (MQ=255)
                                             ttCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgataga                                                                                                   <  2:575477/148‑1 (MQ=255)
                                              tCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatagat                                                                                                  <  1:3426842/148‑1 (MQ=255)
                                              tCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatagat                                                                                                  <  1:3426887/148‑1 (MQ=255)
                                               cGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatagata                                                                                                 <  2:1318164/148‑1 (MQ=255)
                                                 ccAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATAcc                                                                                               >  2:2321265/1‑148 (MQ=255)
                                                 ccAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATAcc                                                                                               >  2:1375093/1‑148 (MQ=255)
                                                   aGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGc                                                                                             >  2:2124629/1‑148 (MQ=255)
                                                   aGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGc                                                                                             >  2:323167/1‑148 (MQ=255)
                                                    gTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCt                                                                                            <  1:1111871/148‑1 (MQ=255)
                                                     tAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCtt                                                                                           >  2:3503724/1‑148 (MQ=255)
                                                      aGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTc                                                                                          >  2:1232913/1‑148 (MQ=255)
                                                        aTTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTg                                                                                        <  2:1751360/148‑1 (MQ=255)
                                                        aTTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTg                                                                                        >  1:2584400/1‑148 (MQ=255)
                                                         ttCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTgg                                                                                       <  1:665595/148‑1 (MQ=255)
                                                          tCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGc                                                                                      <  2:1743931/148‑1 (MQ=255)
                                                                gggCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTc                                                                                <  2:2010870/148‑1 (MQ=255)
                                                                 ggCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTcc                                                                               >  2:1069507/1‑148 (MQ=255)
                                                                 ggCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTcc                                                                               <  2:1579851/148‑1 (MQ=255)
                                                                      aaTCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCAc                                                                          >  1:807730/1‑148 (MQ=255)
                                                                         cAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTc                                                                       >  2:217578/1‑148 (MQ=255)
                                                                            cATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGgt                                                                    <  2:2034087/148‑1 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGgtg                                                                   <  1:3389118/148‑1 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGgtg                                                                   <  1:2626396/148‑1 (MQ=255)
                                                                                 ttCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGa                                                               >  1:2545244/1‑148 (MQ=255)
                                                                                 ttCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGa                                                               <  1:1534317/148‑1 (MQ=255)
                                                                                  tCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGat                                                              <  2:1218687/148‑1 (MQ=255)
                                                                                   cGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGata                                                             >  2:3376737/1‑148 (MQ=255)
                                                                                    gCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGatat                                                            <  2:2073640/148‑1 (MQ=255)
                                                                                      cAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCa                                                          >  1:2478509/1‑148 (MQ=255)
                                                                                       aGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCAc                                                         <  1:2361801/148‑1 (MQ=255)
                                                                                       aGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCAc                                                         <  1:2362239/148‑1 (MQ=255)
                                                                                        gCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACt                                                        >  1:1602156/1‑148 (MQ=255)
                                                                                         cTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTg                                                       <  1:3021795/148‑1 (MQ=255)
                                                                                           ccTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGc                                                     >  2:204135/1‑148 (MQ=255)
                                                                                             tGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCaa                                                   >  1:66363/1‑148 (MQ=255)
                                                                                               acgcacgcGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAac                                                 >  1:667705/1‑148 (MQ=255)
                                                                                               acgcacgcGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACAGGCAAac                                                 >  1:2713044/1‑148 (MQ=255)
                                                                                                    cgcgTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACttt                                            >  2:2859111/1‑148 (MQ=255)
                                                                                                     gcgTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTc                                           <  2:810488/148‑1 (MQ=255)
                                                                                                     gcgTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTATTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTc                                           <  2:2305186/148‑1 (MQ=255)
                                                                                                        tACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAg                                        >  1:1825764/1‑148 (MQ=255)
                                                                                                        tACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAg                                        >  1:1999059/1‑148 (MQ=255)
                                                                                                         aCCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGc                                       >  2:2188160/1‑148 (MQ=255)
                                                                                                           cAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGt                                     >  2:3483908/1‑148 (MQ=255)
                                                                                                            aGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTc                                    >  1:2683644/1‑148 (MQ=255)
                                                                                                              cccTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCg                                  >  1:2006579/1‑148 (MQ=255)
                                                                                                              cccTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCg                                  >  2:1284037/1‑148 (MQ=255)
                                                                                                              cccTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCg                                  >  2:855134/1‑148 (MQ=255)
                                                                                                              cccTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCg                                  >  2:3032469/1‑148 (MQ=255)
                                                                                                              cccTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCg                                  >  2:855270/1‑148 (MQ=255)
                                                                                                                cTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCggg                                <  2:2795140/148‑1 (MQ=255)
                                                                                                                cTTCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCggg                                <  2:1041951/148‑1 (MQ=255)
                                                                                                                  tCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGgcg                              <  2:1615781/148‑1 (MQ=255)
                                                                                                                  tCATGCGGCCCCGTCCCTCGCATGAAATACCTTTGCAGGTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGgcg                              <  2:1615753/148‑1 (MQ=255)
                                                                                                                       cGGCCCCGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTg                         >  2:2645613/1‑148 (MQ=255)
                                                                                                                           cccGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCa                     <  1:1345793/148‑1 (MQ=255)
                                                                                                                           cccGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCa                     <  1:1428488/148‑1 (MQ=255)
                                                                                                                             cGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAt                   >  1:856566/1‑148 (MQ=255)
                                                                                                                             cGTCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTGCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAt                   >  2:68256/1‑148 (MQ=255)
                                                                                                                               tCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAtgt                 >  1:37923/1‑148 (MQ=255)
                                                                                                                               tCCCTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAtgt                 >  1:2916088/1‑148 (MQ=255)
                                                                                                                                cccTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAtgtg                >  1:382327/1‑148 (MQ=255)
                                                                                                                                cccTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAAtgtg                >  2:687700/1‑148 (MQ=255)
                                                                                                                                 ccTCGCATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATgtgt               >  2:1778960/1‑148 (MQ=255)
                                                                                                                                      cATGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATGTGTcagcc          <  2:251959/148‑1 (MQ=255)
                                                                                                                                        tGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATgtgt               <  1:2703795/141‑1 (MQ=255)
                                                                                                                                        tGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATgtgt               >  2:2703795/1‑141 (MQ=255)
                                                                                                                                        tGAAATACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATGTGTcagccag        >  1:3405493/1‑148 (MQ=255)
                                                                                                                                          aaaTACCTTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATGTGTCAGCCAGCg      >  2:3209676/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGATACCGCTTCTGGCTTTTTCCAGCACTTCGGTGTCGATATCACTGGCAAACACTTTCCAGCGTCCGGGCGCGGTGCCCAATGTGTCAGCCAGCGTCAT  >  CP009273/1962930‑1963219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑