Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 100% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA99.3% 472.8 / ‑4.1 170L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (77/87);  total (81/89)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002210‑2002498
                                                                                                                                                |                                                                                                                                                
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:301834/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:326591/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:301788/148‑1 (MQ=255)
tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  1:1280093/148‑1 (MQ=255)
   cATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGCTg                                                                                                                                            >  2:1614393/1‑148 (MQ=255)
     tCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGAGGCt                                                                                                                                          >  2:2682279/1‑148 (MQ=255)
      cGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTg                                                                                                                                         >  1:247008/1‑148 (MQ=255)
          aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCt                                                                                                                                     <  2:625927/148‑1 (MQ=255)
          aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCt                                                                                                                                     >  2:1506665/1‑148 (MQ=255)
           cTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                    >  2:3480422/1‑148 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:3465383/148‑1 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:2965727/148‑1 (MQ=255)
            tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:3465337/148‑1 (MQ=255)
                aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  2:2566667/1‑148 (MQ=255)
                aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  2:2566153/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                              >  2:176297/1‑148 (MQ=255)
                 ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTTGTGTAGGGGTGCTGGTGTTTTTCgg                                                                                                                              >  1:2654687/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             <  1:3251530/148‑1 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             >  2:2392694/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             >  2:2146497/1‑148 (MQ=255)
                  tGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGc                                                                                                                             <  1:481587/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  2:2230614/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  1:3302044/148‑1 (MQ=255)
                   ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                            <  1:3302285/148‑1 (MQ=255)
                     ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          >  2:703641/1‑148 (MQ=255)
                     ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          <  2:769394/148‑1 (MQ=255)
                          ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGACTAGGGGTGCTGGTCTTTTTAGGCATgcgggg                                                                                                                     >  1:1699378/1‑148 (MQ=255)
                          ggTTTGGCGCGCGCTTCACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATgctggg                                                                                                                     >  1:238473/1‑148 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                              <  2:901822/148‑1 (MQ=255)
                                 cgcgcgcTTTACCCTGCTGCCCATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                              <  2:1502907/148‑1 (MQ=255)
                                   cgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgct                                                                                                            >  2:2712444/1‑148 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                         <  2:2510250/148‑1 (MQ=255)
                                      gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCgctgct                                                                                                         >  2:841165/1‑148 (MQ=255)
                                        tttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTca                                                                                                       <  1:1379413/148‑1 (MQ=255)
                                        tttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGGTTCTGGTCTTTTTCGGCCAGCTGGGCTGGGCGCTGCTca                                                                                                       >  1:2339738/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGGGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                      >  1:1129311/1‑148 (MQ=255)
                                         ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                      >  1:1117008/1‑148 (MQ=255)
                                           aCCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAg                                                                                                    <  2:2666307/148‑1 (MQ=255)
                                              ctgctgCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGa                                                                                                 <  2:969935/148‑1 (MQ=255)
                                                   gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                            >  1:2845910/1‑148 (MQ=255)
                                                     ccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTATTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGAAGaacca                                                                                          >  1:3078316/1‑148 (MQ=255)
                                                       aTCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaac                                                                                        <  2:2916479/148‑1 (MQ=255)
                                                           tCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCATGGGATGCGGGTGTTTTTCGGCCTGATGGGGGGGGCGCTGCGCACAGCGAGGAACCAACCCac                                                                                    >  1:1674051/1‑147 (MQ=255)
                                                                aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               >  2:1942911/1‑148 (MQ=255)
                                                                aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               >  2:157500/1‑148 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              <  1:1170205/148‑1 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              <  1:951952/148‑1 (MQ=255)
                                                                 ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              >  2:1733630/1‑148 (MQ=255)
                                                                   cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCCAACAGGGGc                                                                            >  1:1292858/1‑148 (MQ=255)
                                                                    ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCt                                                                           <  1:3200514/148‑1 (MQ=255)
                                                                    ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGCTGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGcg                                                                           >  1:532591/1‑147 (MQ=255)
                                                                      gTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTCGGGCGGCGGGTCTTTTTCGGCCTGGTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGGCTgg                                                                         >  2:2952721/1‑148 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                    <  2:647433/148‑1 (MQ=255)
                                                                           aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                    <  2:3361768/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  >  2:2872208/1‑148 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  <  1:2566667/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCt                                                                  <  1:2566153/148‑1 (MQ=255)
                                                                             tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTTGGGGTGGGGGTGTTTTTTGGGATTCGGGGGGGGGCGGGGCGCACACCCATGTAACAAACCAAAAAGGGGGGGGCCATGcg                                                                  >  1:893351/1‑147 (MQ=255)
                                                                              gCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGCTAGGGGTGGTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGGCATGCTg                                                                 >  1:2264437/1‑148 (MQ=255)
                                                                                 gAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATTCGGGTGTTTTTCGGCGTGGTGGGCTGGGCGCAGCTCAAAGCGATGAACCAACCCCAACGGGGGCGGGCACAGCGGttt                                                              >  2:2107576/1‑148 (MQ=255)
                                                                                   aaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCGGGCAATGCTGTTTgg                                                            >  2:2780838/1‑148 (MQ=255)
                                                                                     gTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTTGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCg                                                          <  1:2107576/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                         <  2:2119678/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                         <  2:1239106/148‑1 (MQ=255)
                                                                                      tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCGGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCGGGCAATGCGGGTTGGCGt                                                         >  2:44709/1‑148 (MQ=255)
                                                                                       ttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCCTGCGGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTc                                                        >  2:163029/1‑148 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:431602/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:3318358/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:1766795/148‑1 (MQ=255)
                                                                                          tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  1:1143127/148‑1 (MQ=255)
                                                                                            ccgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCtt                                                   <  1:1768347/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2505807/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2505731/148‑1 (MQ=255)
                                                                                                 tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGtt                                              <  2:2473381/148‑1 (MQ=255)
                                                                                                    ccGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTGTCGTGTAGGGATGCTGGTGTTTTTCGGCCTTCGGGGCTGGGCGCTGCTCACAGCGAGGAACCAACCCAAACTGGGGCTGGCAATGCGGTTTGGCGGCGGCTTTGGTTTAc                                           >  1:1392381/1‑148 (MQ=255)
                                                                                                       cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGa                                        <  1:1974222/148‑1 (MQ=255)
                                                                                                        tGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                       <  2:2481029/148‑1 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGGGGGTCTTTTTGGGCATGCGGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAAAAGGGGCGGGCCAAGCTGTTTGGCGTCGGGGGTGGTTTAATTATTg                                     >  1:3087216/1‑148 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCGTTGGTTTACTGATTg                                     >  2:3137211/1‑148 (MQ=255)
                                                                                                          aCGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTGGTGGTCTTTTTGGGGATGCTGGGGGGGGCGCTGCTCACAGCGCTGAAACAACCCCAACAGGGGCGGGCAATGCTGTTTGGCGTCGGGGTTGGTTTAATGtttt                                     >  1:3087172/1‑147 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                               <  1:3256322/148‑1 (MQ=255)
                                                                                                                aaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTg                               >  2:274777/1‑148 (MQ=255)
                                                                                                                 aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                              <  1:3141924/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  2:3207806/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  1:2146497/148‑1 (MQ=255)
                                                                                                                     ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                          <  2:867728/148‑1 (MQ=255)
                                                                                                                          aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTg                     <  2:2408271/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAg          <  2:474168/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAg          <  2:1343169/148‑1 (MQ=255)
                                                                                                                                     cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTAATTCAg          >  2:1572944/1‑148 (MQ=255)
                                                                                                                                       ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg        >  2:3494132/1‑148 (MQ=255)
                                                                                                                                       ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg        <  1:1614393/148‑1 (MQ=255)
                                                                                                                                         ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt      <  2:61687/148‑1 (MQ=255)
                                                                                                                                          tcttctaGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  2:2627403/145‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  1:727963/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     >  2:1030718/1‑148 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     <  1:1007735/148‑1 (MQ=255)
                                                                                                                                          tCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTc     >  2:1030976/1‑148 (MQ=255)
                                                                                                                                           cGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTcc    >  1:2484967/1‑148 (MQ=255)
                                                                                                                                            gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcg   <  2:2956285/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002210‑2002498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑