Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,346 C→A 100% I976I (ATC→ATA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,3460CA98.4% 750.9 / 3.0 243I976I (ATC→ATA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (128/111);  total (130/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

AGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAA  >  CP009273/2041201‑2041489
                                                                                                                                                 |                                                                                                                                               
aGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAc                                                                                                                                               <  2:2598425/148‑1 (MQ=255)
 ggTTAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAcc                                                                                                                                              <  2:1999588/148‑1 (MQ=255)
 ggTGAATTTTATCGGTGATCAAAGTACTGCTGACCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAcc                                                                                                                                              >  1:1373773/1‑148 (MQ=255)
  gTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                             >  1:825574/1‑148 (MQ=255)
  gTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACGAAAAGATAAAGAAATAACCt                                                                                                                                             >  2:1173130/1‑148 (MQ=255)
      aTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCTTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtctc                                                                                                                                         <  2:3024929/148‑1 (MQ=255)
        tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtg                                                                                                                                       >  1:492157/1‑148 (MQ=255)
              ggTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCaa                                                                                                                                 <  2:2964759/148‑1 (MQ=255)
               gTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCaaa                                                                                                                                <  2:2791467/148‑1 (MQ=255)
                  aTCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacga                                                                                                                             >  2:654331/1‑148 (MQ=255)
                       aGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCg                                                                                                                        <  2:1029087/148‑1 (MQ=255)
                       aGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCg                                                                                                                        >  1:2867408/1‑148 (MQ=255)
                           ctgctgCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaag                                                                                                                    <  2:1772821/148‑1 (MQ=255)
                              ctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaa                                                                                                                 >  1:806651/1‑148 (MQ=255)
                              ctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaa                                                                                                                 >  1:807469/1‑148 (MQ=255)
                                gcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaagt                                                                                                               >  2:780610/1‑148 (MQ=255)
                                 cccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGtt                                                                                                              >  2:388741/1‑148 (MQ=255)
                                 cccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGtt                                                                                                              >  2:389393/1‑148 (MQ=255)
                                  ccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtc                                                                                                             <  2:2089776/148‑1 (MQ=255)
                                   cTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtct                                                                                                            >  2:1466140/1‑148 (MQ=255)
                                    tGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtctc                                                                                                           >  1:289596/1‑148 (MQ=255)
                                     gACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCg                                                                                                          >  2:1921599/1‑148 (MQ=255)
                                      aCCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGa                                                                                                         <  1:2619630/148‑1 (MQ=255)
                                      aCCCTCAGTGTGCCTTCAGGTGAGATCACCGTCACCAACACAGCACCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGa                                                                                                         <  2:3461821/148‑1 (MQ=255)
                                       cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAt                                                                                                        >  1:323627/1‑148 (MQ=255)
                                           cAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGc                                                                                                    >  2:2205879/1‑148 (MQ=255)
                                            aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCa                                                                                                   <  1:1586583/148‑1 (MQ=255)
                                              tgtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc                                                                                                 >  1:2502500/1‑148 (MQ=255)
                                              tgtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc                                                                                                 >  1:2734544/1‑148 (MQ=255)
                                                 gCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgga                                                                                              <  1:437950/148‑1 (MQ=255)
                                                    ttCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggagga                                                                                           >  1:1311689/1‑148 (MQ=255)
                                                    ttCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGAAAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggagga                                                                                           <  1:330609/148‑1 (MQ=255)
                                                      cAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGaaa                                                                                         <  1:2729853/148‑1 (MQ=255)
                                                         gTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                      >  1:357991/1‑148 (MQ=255)
                                                         gTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                      >  2:3420166/1‑148 (MQ=255)
                                                          tGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGc                                                                                     >  1:417337/1‑148 (MQ=255)
                                                           gATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCa                                                                                    <  1:3401928/148‑1 (MQ=255)
                                                           gATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAACAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCa                                                                                    <  1:1549338/148‑1 (MQ=255)
                                                             tatCACCGTCACCAACACAGCTCCGCAATATAGGACTGCAACCTTGCAGTATAAAAATGGCAACCCACTAAAAGATAAAGAACTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                  <  1:2653995/148‑1 (MQ=255)
                                                               tCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGAc                                                                                >  2:2144229/1‑148 (MQ=255)
                                                                   cGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGAt                                                                            >  2:1225200/1‑148 (MQ=255)
                                                                     tCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAg                                                                          >  1:625267/1‑148 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                        <  1:2430395/148‑1 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                        <  1:1993658/148‑1 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                        <  2:1351451/148‑1 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                        <  1:2129001/148‑1 (MQ=255)
                                                                        ccAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTaa                                                                       <  1:2012180/148‑1 (MQ=255)
                                                                         cAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAAc                                                                      >  2:2563080/1‑148 (MQ=255)
                                                                            cacaGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                   <  1:1759719/148‑1 (MQ=255)
                                                                            cacaGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                   >  2:1874312/1‑148 (MQ=255)
                                                                             acaGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACgggg                                                                  <  2:2649819/148‑1 (MQ=255)
                                                                              caGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGt                                                                 <  1:972855/148‑1 (MQ=255)
                                                                              caGCTCCGCAATATAGGACTGCAACCTTGCAGGATAAAAAGGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGt                                                                 <  1:725203/148‑1 (MQ=255)
                                                                               aGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGACAAGGCATGACGGATAGTAACGGGGtt                                                                >  2:2199556/1‑148 (MQ=255)
                                                                                   ccGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCaa                                                            <  2:2359960/148‑1 (MQ=255)
                                                                                      cAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCg                                                         >  2:3396036/1‑148 (MQ=255)
                                                                                         tatatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCt                                                      >  2:3508023/1‑148 (MQ=255)
                                                                                         tatatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCt                                                      <  1:3164921/148‑1 (MQ=255)
                                                                                         tatatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAACAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCt                                                      <  1:1446889/148‑1 (MQ=255)
                                                                                           tatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTcc                                                    <  1:2496931/148‑1 (MQ=255)
                                                                                            atGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTccc                                                   >  1:1657950/1‑148 (MQ=255)
                                                                                              gACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTg                                                 >  1:1193002/1‑148 (MQ=255)
                                                                                               aCTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGa                                                >  1:75624/1‑148 (MQ=255)
                                                                                                  gCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTGGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCg                                             >  1:362393/1‑148 (MQ=255)
                                                                                                     aCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCa                                          >  2:3052735/1‑148 (MQ=255)
                                                                                                     aCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCa                                          >  1:1774387/1‑148 (MQ=255)
                                                                                                      ccTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCAc                                         >  1:1402034/1‑148 (MQ=255)
                                                                                                        ttGCATGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGt                                       <  2:1820146/148‑1 (MQ=255)
                                                                                                         tGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGtt                                      <  1:2258763/148‑1 (MQ=255)
                                                                                                          gCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTa                                     <  1:2261540/148‑1 (MQ=255)
                                                                                                             ggATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCg                                  >  2:3015810/1‑148 (MQ=255)
                                                                                                             ggATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCg                                  >  2:322575/1‑148 (MQ=255)
                                                                                                                tAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGc                               >  1:1893340/1‑148 (MQ=255)
                                                                                                                 aaaaaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCa                              >  1:2274250/1‑148 (MQ=255)
                                                                                                                    aaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGc                           >  2:1637980/1‑148 (MQ=255)
                                                                                                                    aaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGc                           >  2:1304918/1‑148 (MQ=255)
                                                                                                                     aTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCa                          <  2:3488354/148‑1 (MQ=255)
                                                                                                                           aCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGAt                    <  2:2172432/148‑1 (MQ=255)
                                                                                                                           aCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGAt                    <  2:2768187/148‑1 (MQ=255)
                                                                                                                            cccACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                   >  2:2034565/1‑148 (MQ=255)
                                                                                                                               aCTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATg                <  2:815394/148‑1 (MQ=255)
                                                                                                                               aCTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATg                >  2:1048820/1‑148 (MQ=255)
                                                                                                                                cTAAAAGATAAAGCACTCACCTTCTCTGTGCCATACGACGTCGCAAGTAAGTTCTCTATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATgg               <  1:2981053/148‑1 (MQ=255)
                                                                                                                                cTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATgg               >  2:2547295/1‑148 (MQ=255)
                                                                                                                                  aaaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCt             >  1:1942152/1‑148 (MQ=255)
                                                                                                                                    taGATAAAGAATTAAGCTTCTCGGTGCCACACGACGTCGCAAGTAAGTTGTCGATTAGCAACGGAGGAAAAGGCATGTCGGATAGTAACGGGGTTGCAATCGCCTCCCTGACGGGCACGTTAGCGGGCACGGATATGATCATGGCTCg           <  2:3170502/147‑1 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt          >  2:216662/1‑148 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt          >  2:606263/1‑148 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt          <  1:2747006/148‑1 (MQ=255)
                                                                                                                                      gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTCACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc         >  2:1958857/1‑148 (MQ=255)
                                                                                                                                      gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc         >  2:2422893/1‑148 (MQ=255)
                                                                                                                                      gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc         >  1:309114/1‑148 (MQ=255)
                                                                                                                                      gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc         >  1:62197/1‑148 (MQ=255)
                                                                                                                                        tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTg       >  1:2358685/1‑148 (MQ=255)
                                                                                                                                         aaaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg      >  1:343176/1‑148 (MQ=255)
                                                                                                                                         aaaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg      >  1:1016811/1‑148 (MQ=255)
                                                                                                                                          aacaaaTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGc     <  1:2368391/145‑1 (MQ=255)
                                                                                                                                          aaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGc     <  1:2197537/148‑1 (MQ=255)
                                                                                                                                          aaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGc     <  1:1173130/148‑1 (MQ=255)
                                                                                                                                             aaaTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTaa  >  1:901933/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAA  >  CP009273/2041201‑2041489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑