Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,093,612 C→A 100% E380* (GAA→TAA)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,093,6120CA99.1% 757.6 / ‑1.9 232E380* (GAA→TAA) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (112/118);  total (113/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

TAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGA  >  CP009273/2093467‑2093756
                                                                                                                                                 |                                                                                                                                                
tAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAgg                                                                                                                                                >  1:1954026/1‑148 (MQ=255)
 aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGc                                                                                                                                               <  2:3450743/148‑1 (MQ=255)
  aTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCa                                                                                                                                              <  2:2708456/148‑1 (MQ=255)
     ggCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATaa                                                                                                                                           <  1:2387661/148‑1 (MQ=255)
     ggCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATaa                                                                                                                                           >  1:2921008/1‑148 (MQ=255)
     ggCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATaa                                                                                                                                           <  1:376903/148‑1 (MQ=255)
       cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGc                                                                                                                                         >  1:1559621/1‑148 (MQ=255)
         aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCAt                                                                                                                                       <  1:2462903/148‑1 (MQ=255)
           cGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCg                                                                                                                                     <  2:1082607/148‑1 (MQ=255)
           cGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCg                                                                                                                                     <  2:203312/148‑1 (MQ=255)
            gCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCgg                                                                                                                                    <  2:2671075/148‑1 (MQ=255)
            gCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCgg                                                                                                                                    <  1:156321/148‑1 (MQ=255)
            gCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCgg                                                                                                                                    <  2:2663244/148‑1 (MQ=255)
               gCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGa                                                                                                                                 <  1:1015599/148‑1 (MQ=255)
                  gagaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGTCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAttt                                                                                                                              <  2:1550433/148‑1 (MQ=255)
                  gagaAGGACGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAttt                                                                                                                              >  2:2934833/1‑148 (MQ=255)
                   agaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAtttt                                                                                                                             <  2:2282026/148‑1 (MQ=255)
                     aaGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCt                                                                                                                           >  2:1714447/1‑148 (MQ=255)
                      aGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTg                                                                                                                          >  2:3314379/1‑148 (MQ=255)
                        gTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCa                                                                                                                        >  2:1902902/1‑148 (MQ=255)
                            gAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGaa                                                                                                                    >  1:654076/1‑148 (MQ=255)
                            gAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGaa                                                                                                                    >  1:2434644/1‑148 (MQ=255)
                             aaCCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAAc                                                                                                                   >  1:2715441/1‑148 (MQ=255)
                              aCCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACt                                                                                                                  >  2:1346005/1‑148 (MQ=255)
                                  gAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTgcgc                                                                                                              <  1:568116/148‑1 (MQ=255)
                                  gAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTgcgc                                                                                                              <  2:1447390/148‑1 (MQ=255)
                                   aaTACCGGTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTCGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACCGGTTAGCGATCTGTGGATTGTAGGCATAAGCATCGCTGATTTTCTGCAGGAACTGCGCa                                                                                                             >  2:1779689/1‑148 (MQ=255)
                                    aTACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCAc                                                                                                            >  1:1874060/1‑148 (MQ=255)
                                      aCCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg                                                                                                          >  1:2577244/1‑148 (MQ=255)
                                       ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGga                                                                                                         <  1:3426884/148‑1 (MQ=255)
                                       ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGga                                                                                                         >  2:2876366/1‑148 (MQ=255)
                                          ttCTGTACTGCGTAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatga                                                                                                      >  2:193564/1‑148 (MQ=255)
                                            cTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGatgatg                                                                                                    <  1:2245261/148‑1 (MQ=255)
                                              gTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGca                                                                                                  >  1:945699/1‑148 (MQ=255)
                                               tACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcag                                                                                                 <  1:2246342/148‑1 (MQ=255)
                                               tACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcag                                                                                                 >  2:2199927/1‑148 (MQ=255)
                                               tACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcag                                                                                                 >  2:2830936/1‑148 (MQ=255)
                                                aCTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                <  2:2807799/148‑1 (MQ=255)
                                                aCTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                >  1:1111005/1‑148 (MQ=255)
                                                   gCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagccag                                                                                             <  1:1061420/148‑1 (MQ=255)
                                                     aTAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCa                                                                                           <  1:2950185/148‑1 (MQ=255)
                                                     aTAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCa                                                                                           >  1:1204698/1‑148 (MQ=255)
                                                      tAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCAc                                                                                          >  2:3348759/1‑148 (MQ=255)
                                                      tAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCAc                                                                                          >  1:478782/1‑148 (MQ=255)
                                                       aaGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACg                                                                                         <  2:3016320/148‑1 (MQ=255)
                                                        aGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACgg                                                                                        <  2:2591269/148‑1 (MQ=255)
                                                        aGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACgg                                                                                        >  1:2155805/1‑148 (MQ=255)
                                                           aaCGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGaaa                                                                                     <  1:1057458/148‑1 (MQ=255)
                                                                cATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCtt                                                                                >  1:88530/1‑148 (MQ=255)
                                                                cATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCtt                                                                                >  1:823001/1‑148 (MQ=255)
                                                                       cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAtt                                                                         >  2:3368526/1‑148 (MQ=255)
                                                                          cgcCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCg                                                                      >  1:3120320/1‑148 (MQ=255)
                                                                            ccTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGcc                                                                    <  2:2852482/148‑1 (MQ=255)
                                                                             ctgctgGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCg                                                                   <  2:619343/148‑1 (MQ=255)
                                                                               gctgGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTa                                                                 <  2:3258442/148‑1 (MQ=255)
                                                                                   gTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTc                                                             <  1:20915/148‑1 (MQ=255)
                                                                                       tCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGAt                                                         <  2:3033834/148‑1 (MQ=255)
                                                                                        cATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATc                                                        <  2:1106935/148‑1 (MQ=255)
                                                                                         aTCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                       >  2:2152163/1‑148 (MQ=255)
                                                                                         aTCGACAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                       >  1:2842849/1‑148 (MQ=255)
                                                                                          tCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATccc                                                      <  2:88530/148‑1 (MQ=255)
                                                                                           cGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCa                                                     >  2:527747/1‑148 (MQ=255)
                                                                                             gCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGt                                                   >  2:650188/1‑148 (MQ=255)
                                                                                              cAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGtt                                                  >  1:2798312/1‑148 (MQ=255)
                                                                                              cAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGtt                                                  <  2:1804680/148‑1 (MQ=255)
                                                                                              cAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGtt                                                  >  1:3266719/1‑148 (MQ=255)
                                                                                               aaTTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTg                                                 <  1:3365146/148‑1 (MQ=255)
                                                                                                 tttGCTTGAAGTACGGAGCCAGCAACAGGTGAGCGATCGGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTa                                               <  2:2291064/148‑1 (MQ=255)
                                                                                                  ttGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTAc                                              <  2:2708636/148‑1 (MQ=255)
                                                                                                  ttGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTAc                                              >  1:14417/1‑148 (MQ=255)
                                                                                                   tGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTAct                                             <  2:735018/148‑1 (MQ=255)
                                                                                                         aaGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAg                                       <  1:2341617/148‑1 (MQ=255)
                                                                                                          aGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGa                                      <  2:1267459/148‑1 (MQ=255)
                                                                                                          aGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGa                                      <  2:2411191/148‑1 (MQ=255)
                                                                                                            tACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacg                                    >  1:2313185/1‑148 (MQ=255)
                                                                                                             aCGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgc                                   >  2:570785/1‑148 (MQ=255)
                                                                                                                 aGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagca                               <  1:1190411/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacg                             <  2:2376714/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacg                             <  2:613886/148‑1 (MQ=255)
                                                                                                                      gCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcag                          >  2:952224/1‑148 (MQ=255)
                                                                                                                       cAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                         >  2:443104/1‑148 (MQ=255)
                                                                                                                       cAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                         >  1:3172816/1‑148 (MQ=255)
                                                                                                                       cAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                         >  1:705933/1‑148 (MQ=255)
                                                                                                                        aaCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCt                        >  1:3065587/1‑148 (MQ=255)
                                                                                                                         aCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                       >  2:2514826/1‑148 (MQ=255)
                                                                                                                         aCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                       >  2:3170653/1‑148 (MQ=255)
                                                                                                                         aCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                       >  2:3170594/1‑148 (MQ=255)
                                                                                                                         aCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                       >  2:2625509/1‑148 (MQ=255)
                                                                                                                            ggTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgaga                    <  2:1861048/148‑1 (MQ=255)
                                                                                                                             gTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgagag                   >  2:1148504/1‑148 (MQ=255)
                                                                                                                             gTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgagag                   >  1:1568090/1‑148 (MQ=255)
                                                                                                                              ttAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgagaga                  <  1:582929/148‑1 (MQ=255)
                                                                                                                                 gCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGc               <  1:803030/148‑1 (MQ=255)
                                                                                                                                  cGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGcc              >  1:393180/1‑148 (MQ=255)
                                                                                                                                  cGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGcc              <  1:956401/148‑1 (MQ=255)
                                                                                                                                     tCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg           >  2:3097998/1‑148 (MQ=255)
                                                                                                                                     tCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg           <  2:3467004/148‑1 (MQ=255)
                                                                                                                                     tCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg           >  2:2362073/1‑148 (MQ=255)
                                                                                                                                           gATTTTAGGCATACGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAATATCTTCNCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTa     <  1:1779689/148‑1 (MQ=255)
                                                                                                                                           gATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTa     >  1:1672734/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGA  >  CP009273/2093467‑2093756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑