Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,110,346 G→A 100% T88I (ACC→ATC)  wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,110,3460GA99.4% 582.3 / ‑4.3 176T88I (ACC→ATC) wcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (84/91);  total (84/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

CGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCG  >  CP009273/2110199‑2110489
                                                                                                                                                   |                                                                                                                                               
cgGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAgg                                                                                                                                                 >  2:1840343/1‑148 (MQ=255)
  gAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATa                                                                                                                                               >  1:2450261/1‑148 (MQ=255)
  gAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGAGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATa                                                                                                                                               >  2:137597/1‑148 (MQ=255)
      tttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtttt                                                                                                                                           >  2:943717/1‑148 (MQ=255)
       ttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttttt                                                                                                                                          <  2:693310/148‑1 (MQ=255)
          ctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACg                                                                                                                                       >  1:481876/1‑148 (MQ=255)
          ctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACg                                                                                                                                       >  1:1970061/1‑148 (MQ=255)
            gcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGAt                                                                                                                                     <  1:3256485/148‑1 (MQ=255)
              ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGa                                                                                                                                   <  1:1413523/148‑1 (MQ=255)
               gTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGaa                                                                                                                                  >  2:1245645/1‑148 (MQ=255)
               gTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGaa                                                                                                                                  <  2:808669/148‑1 (MQ=255)
               gTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGaa                                                                                                                                  <  1:1136425/148‑1 (MQ=255)
                ttACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                 >  1:1726994/1‑148 (MQ=255)
                ttACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                 <  1:1182978/148‑1 (MQ=255)
                  aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                               >  2:711750/1‑148 (MQ=255)
                  aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                               >  2:2920617/1‑148 (MQ=255)
                  aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                               >  2:2302815/1‑148 (MQ=255)
                  aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                               >  1:987739/1‑148 (MQ=255)
                    ccccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcg                                                                                                                             >  2:1216582/1‑148 (MQ=255)
                            gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     <  1:1030115/148‑1 (MQ=255)
                            gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     <  1:1037908/148‑1 (MQ=255)
                            gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     >  2:1265094/1‑148 (MQ=255)
                            gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     <  1:742982/148‑1 (MQ=255)
                             aCCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTc                                                                                                                    <  1:1797801/148‑1 (MQ=255)
                              ccAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGGTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAACGTct                                                                                                                   >  2:2116845/1‑148 (MQ=255)
                                 aaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                <  2:3101048/148‑1 (MQ=255)
                                 aaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                <  2:2220202/148‑1 (MQ=255)
                                  aaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggct                                                                                                               >  1:2262443/1‑148 (MQ=255)
                                            gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGAGACTCGGCACCATATCGTTGGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTgg                                                                                                     >  2:712286/1‑148 (MQ=255)
                                              acacATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcg                                                                                                   >  2:1319804/1‑148 (MQ=255)
                                               cacaTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcgc                                                                                                  >  2:2791320/1‑148 (MQ=255)
                                                 caTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAg                                                                                                >  1:725795/1‑148 (MQ=255)
                                                  aTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGt                                                                                               <  2:3310801/148‑1 (MQ=255)
                                                   tCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGtt                                                                                              <  2:1416587/148‑1 (MQ=255)
                                                    cGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGttt                                                                                             >  1:802464/1‑148 (MQ=255)
                                                         cgAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCa                                                                                        >  2:3346909/1‑148 (MQ=255)
                                                         cgAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCa                                                                                        <  2:545967/148‑1 (MQ=255)
                                                            aaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                     <  2:1726994/148‑1 (MQ=255)
                                                               ggTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGc                                                                                  <  2:2696088/148‑1 (MQ=255)
                                                                     gCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCGTAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAg                                                                            <  1:1410737/148‑1 (MQ=255)
                                                                     gCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAg                                                                            <  1:1410771/148‑1 (MQ=255)
                                                                      cGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAgg                                                                           <  2:2917550/148‑1 (MQ=255)
                                                                      cGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAgg                                                                           <  2:1167650/148‑1 (MQ=255)
                                                                          cTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCg                                                                       >  2:1303933/1‑148 (MQ=255)
                                                                            ggCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTc                                                                     <  1:211339/148‑1 (MQ=255)
                                                                            ggCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTc                                                                     <  1:2975780/148‑1 (MQ=255)
                                                                             gCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTcc                                                                    <  1:3084110/148‑1 (MQ=255)
                                                                                gCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGt                                                                 >  1:3021258/1‑148 (MQ=255)
                                                                                 cAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTa                                                                >  2:1444406/1‑148 (MQ=255)
                                                                                  aaaTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTaa                                                               >  1:79758/1‑148 (MQ=255)
                                                                                   aaTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                                              >  1:820244/1‑148 (MQ=255)
                                                                                   aaTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                                              <  1:3350642/148‑1 (MQ=255)
                                                                                      ggCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAg                                                           >  2:1587715/1‑148 (MQ=255)
                                                                                          gACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCggg                                                       <  1:2169136/148‑1 (MQ=255)
                                                                                           aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCTGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                      >  1:1513347/1‑148 (MQ=255)
                                                                                           aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                      <  1:711750/148‑1 (MQ=255)
                                                                                           aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                      >  2:368411/1‑148 (MQ=255)
                                                                                            cAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                     >  2:1776372/1‑148 (MQ=255)
                                                                                            cAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                     <  1:2841419/148‑1 (MQ=255)
                                                                                            cAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                     <  1:1358492/148‑1 (MQ=255)
                                                                                             aaaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCNCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTc                                                    <  1:1784328/148‑1 (MQ=255)
                                                                                              aaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCt                                                   >  2:433983/1‑148 (MQ=255)
                                                                                               aaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTg                                                  <  2:3443092/148‑1 (MQ=255)
                                                                                                aTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTgg                                                 >  1:2923246/1‑148 (MQ=255)
                                                                                                  cAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGgca                                               >  2:1572898/1‑148 (MQ=255)
                                                                                                      ttCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                           <  2:2319719/148‑1 (MQ=255)
                                                                                                          gcgACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGtt                                       <  2:2079659/148‑1 (MQ=255)
                                                                                                          gcgACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGtt                                       <  2:1374952/148‑1 (MQ=255)
                                                                                                           cgAGTCGGCACCATAGCGTTTGATGTTGATCGCCTGCCAGATATTTTTACGATTATTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAATGTTg                                      <  2:348536/148‑1 (MQ=255)
                                                                                                             aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTa                                    <  1:1291631/148‑1 (MQ=255)
                                                                                                             aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTa                                    <  1:685189/148‑1 (MQ=255)
                                                                                                             aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTa                                    <  2:2706121/148‑1 (MQ=255)
                                                                                                              cTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAt                                   <  1:2399323/148‑1 (MQ=255)
                                                                                                              cTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAt                                   <  1:2399305/148‑1 (MQ=255)
                                                                                                               tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                  <  2:3115785/148‑1 (MQ=255)
                                                                                                               tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                  <  2:3115804/148‑1 (MQ=255)
                                                                                                                 ggCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTc                                >  2:3179976/1‑148 (MQ=255)
                                                                                                                  gCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg                               >  1:2684303/1‑148 (MQ=255)
                                                                                                                     ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTcc                            >  2:2814275/1‑148 (MQ=255)
                                                                                                                       aTAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCAt                          >  2:2524475/1‑148 (MQ=255)
                                                                                                                        tAGCGTTTGAGTTTGAGCGCCTGACATATATTTTTTCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGTTTCTGGCAGCAAGGTTGTATTTCGTCCATg                         <  2:1754400/148‑1 (MQ=255)
                                                                                                                        tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATg                         <  2:2728575/148‑1 (MQ=255)
                                                                                                                         aGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        >  1:3429138/1‑148 (MQ=255)
                                                                                                                          gCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGcc                       >  2:3341530/1‑148 (MQ=255)
                                                                                                                               tGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTg                  <  1:3102646/148‑1 (MQ=255)
                                                                                                                               tGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTg                  >  1:2232675/1‑148 (MQ=255)
                                                                                                                               tGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTg                  >  1:464133/1‑148 (MQ=255)
                                                                                                                                gAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTgg                 <  2:25691/148‑1 (MQ=255)
                                                                                                                                 aGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTggg                >  2:1502159/1‑148 (MQ=255)
                                                                                                                                   gTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtg              >  2:1082662/1‑148 (MQ=255)
                                                                                                                                   gTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtg              >  1:1012717/1‑148 (MQ=255)
                                                                                                                                    ttGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGGGGgtgt             >  2:2993122/1‑148 (MQ=255)
                                                                                                                                     tGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtt            <  1:1265094/148‑1 (MQ=255)
                                                                                                                                     tGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtt            >  2:73169/1‑148 (MQ=255)
                                                                                                                                         cgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgt        >  2:3086163/1‑148 (MQ=255)
                                                                                                                                         cgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgt        >  1:225785/1‑148 (MQ=255)
                                                                                                                                          gcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtg       <  1:2226008/148‑1 (MQ=255)
                                                                                                                                           ccTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgt      <  1:943717/148‑1 (MQ=255)
                                                                                                                                            cTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgtg     <  1:2906133/148‑1 (MQ=255)
                                                                                                                                               ccAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCg  >  2:686707/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCG  >  CP009273/2110199‑2110489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑