Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,141,613 G→T 100% V153V (GTG→GTT)  yegD → Hsp70 chaperone family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,141,6130GT99.4% 617.8 / ‑4.2 195V153V (GTG→GTT) yegDHsp70 chaperone family protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (97/96);  total (99/96)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

TTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTA  >  CP009273/2141467‑2141756
                                                                                                                                                  |                                                                                                                                               
 ttGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTAt                                                                                                                                               >  1:3488173/1‑148 (MQ=255)
 ttGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTAt                                                                                                                                               <  1:231518/148‑1 (MQ=255)
 ttGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTAt                                                                                                                                               <  1:3333603/148‑1 (MQ=255)
    tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTgg                                                                                                                                            >  1:2612513/1‑148 (MQ=255)
      aaaTCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGgtc                                                                                                                                          <  2:852258/148‑1 (MQ=255)
        aTCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGgtcgt                                                                                                                                        >  2:738024/1‑148 (MQ=255)
          cACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTcc                                                                                                                                      >  2:1763390/1‑148 (MQ=255)
              aaaaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATc                                                                                                                                  >  2:3183096/1‑148 (MQ=255)
                aaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCaa                                                                                                                                <  1:276343/148‑1 (MQ=255)
                 aTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAAc                                                                                                                               <  2:1953398/148‑1 (MQ=255)
                 aTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAAc                                                                                                                               >  2:485100/1‑148 (MQ=255)
                 aTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAAc                                                                                                                               >  2:2533040/1‑148 (MQ=255)
                  tCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACt                                                                                                                              <  2:2456749/148‑1 (MQ=255)
                  tCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACt                                                                                                                              <  2:1425931/148‑1 (MQ=255)
                   cGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACtt                                                                                                                             <  2:622465/148‑1 (MQ=255)
                   cGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACtt                                                                                                                             <  2:1300989/148‑1 (MQ=255)
                    gTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTc                                                                                                                            >  2:1154358/1‑148 (MQ=255)
                    gTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTc                                                                                                                            <  1:488991/148‑1 (MQ=255)
                       ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAg                                                                                                                         >  2:3422410/1‑148 (MQ=255)
                       ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAg                                                                                                                         >  2:2790761/1‑148 (MQ=255)
                       ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAg                                                                                                                         <  1:3092885/148‑1 (MQ=255)
                        cTCGGTGCCAGCGGCTTGAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAAc                                                                                                                               <  1:2809278/141‑1 (MQ=255)
                         tCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAggg                                                                                                                       >  2:592598/1‑148 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                      <  1:2466393/148‑1 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                      >  1:3328875/1‑148 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                      <  1:1898868/148‑1 (MQ=255)
                          cGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgggg                                                                                                                      <  1:123028/148‑1 (MQ=255)
                            gTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCt                                                                                                                    <  1:2290112/148‑1 (MQ=255)
                            gTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCt                                                                                                                    >  1:681898/1‑148 (MQ=255)
                             tGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTg                                                                                                                   >  1:1005924/1‑148 (MQ=255)
                             tGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTg                                                                                                                   >  1:1006481/1‑148 (MQ=255)
                               ccAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTggg                                                                                                                 <  1:754076/148‑1 (MQ=255)
                                 aGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCg                                                                                                               >  1:243847/1‑148 (MQ=255)
                                  gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                              >  2:2319186/1‑148 (MQ=255)
                                   cGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGt                                                                                                             <  1:368638/148‑1 (MQ=255)
                                       ttAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGtgatg                                                                                                         <  1:3188219/148‑1 (MQ=255)
                                       ttAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGtgatg                                                                                                         <  2:2612513/148‑1 (MQ=255)
                                             ccGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCaa                                                                                                   >  2:337256/1‑148 (MQ=255)
                                               gcagcaGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAac                                                                                                 <  2:1065606/148‑1 (MQ=255)
                                                   cagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAccc                                                                                             >  1:203950/1‑148 (MQ=255)
                                                      gTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAgg                                                                                          >  2:2097851/1‑148 (MQ=255)
                                                      gTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAgg                                                                                          >  2:2161741/1‑148 (MQ=255)
                                                           gcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCaa                                                                                     <  1:1932508/148‑1 (MQ=255)
                                                           gcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCaa                                                                                     <  1:1551620/148‑1 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                    >  1:386635/1‑148 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                    <  1:1480271/148‑1 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                    >  1:1322943/1‑148 (MQ=255)
                                                            cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                    <  2:2275126/148‑1 (MQ=255)
                                                              gTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAggg                                                                                  >  2:2131840/1‑148 (MQ=255)
                                                              gTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAggg                                                                                  <  2:3054469/148‑1 (MQ=255)
                                                               tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                                 >  1:535381/1‑148 (MQ=255)
                                                               tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                                 <  2:1321098/148‑1 (MQ=255)
                                                                 tGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGAtt                                                                               <  2:870518/148‑1 (MQ=255)
                                                                 tGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGAtt                                                                               >  2:846931/1‑148 (MQ=255)
                                                                   aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCAGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCt                                                                             >  1:2393028/1‑148 (MQ=255)
                                                                     gATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTgg                                                                           >  2:2558743/1‑148 (MQ=255)
                                                                      aTCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGa                                                                          <  1:1702472/148‑1 (MQ=255)
                                                                         tGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcg                                                                       <  2:2401391/148‑1 (MQ=255)
                                                                           gTCTGCGCAATGATGTTGCACAGTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAcgcg                                                                     <  2:2756564/148‑1 (MQ=255)
                                                                                cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggcg                                                                <  2:535381/148‑1 (MQ=255)
                                                                                  cAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGaa                                                              <  2:242632/148‑1 (MQ=255)
                                                                                  cAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGaa                                                              >  2:2555971/1‑148 (MQ=255)
                                                                                   aaTGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAg                                                             >  1:3010462/1‑148 (MQ=255)
                                                                                    atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGc                                                            >  2:1041536/1‑148 (MQ=255)
                                                                                    atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGc                                                            >  2:2943198/1‑148 (MQ=255)
                                                                                    atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCAGGAACGCGCGGCGAAGc                                                            >  1:2841176/1‑148 (MQ=255)
                                                                                      gatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGt                                                          >  2:1599526/1‑148 (MQ=255)
                                                                                       atgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTg                                                         <  1:918968/148‑1 (MQ=255)
                                                                                        tgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGc                                                        <  1:409496/148‑1 (MQ=255)
                                                                                         gTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGcc                                                       >  1:946760/1‑148 (MQ=255)
                                                                                           tGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCgg                                                     >  2:1299379/1‑148 (MQ=255)
                                                                                           tGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCgg                                                     >  1:131375/1‑148 (MQ=255)
                                                                                            gCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGa                                                    <  2:1668836/148‑1 (MQ=255)
                                                                                             cacaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAt                                                   <  2:677141/148‑1 (MQ=255)
                                                                                              acaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAtt                                                  <  2:2817660/148‑1 (MQ=255)
                                                                                                aTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCa                                                <  1:1154358/148‑1 (MQ=255)
                                                                                                aTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCa                                                >  2:933007/1‑148 (MQ=255)
                                                                                                   cGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAggg                                             <  1:2804389/148‑1 (MQ=255)
                                                                                                     ccAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGAc                                           <  1:1288174/148‑1 (MQ=255)
                                                                                                      cagcagGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACg                                          <  1:24143/148‑1 (MQ=255)
                                                                                                       agcagGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGt                                         <  1:3179828/148‑1 (MQ=255)
                                                                                                              gcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTc                                  <  2:1793560/148‑1 (MQ=255)
                                                                                                               caggcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTcc                                 <  2:2387732/148‑1 (MQ=255)
                                                                                                                  gcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGt                              >  2:339347/1‑148 (MQ=255)
                                                                                                                  gcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGt                              >  1:677181/1‑148 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                           <  1:2533040/148‑1 (MQ=255)
                                                                                                                     caGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACg                           >  2:829500/1‑148 (MQ=255)
                                                                                                                      aGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGa                          <  1:1594187/148‑1 (MQ=255)
                                                                                                                       gCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAg                         <  1:2227254/148‑1 (MQ=255)
                                                                                                                       gCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAg                         <  1:1994900/148‑1 (MQ=255)
                                                                                                                       gCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAg                         <  1:2958752/148‑1 (MQ=255)
                                                                                                                           ccAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCgg                     <  2:1250730/148‑1 (MQ=255)
                                                                                                                               aaGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc                 >  2:1975367/1‑148 (MQ=255)
                                                                                                                                 gCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCCGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCgg               <  2:550769/148‑1 (MQ=255)
                                                                                                                                   gATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCNAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggct             <  1:1781875/148‑1 (MQ=255)
                                                                                                                                   gATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggct             <  1:846931/148‑1 (MQ=255)
                                                                                                                                   gATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggct             <  1:3252297/148‑1 (MQ=255)
                                                                                                                                    aTTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACTAGCCGGTCGCggctg            >  2:2699394/1‑148 (MQ=255)
                                                                                                                                        cTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctgggct        >  2:972752/1‑148 (MQ=255)
                                                                                                                                              cGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTa  >  1:1708620/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTA  >  CP009273/2141467‑2141756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑