Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,149,603 A→T 100% E287V (GAA→GTA)  mdtB → multidrug efflux system, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,149,6030AT100.0% 775.9 / NA 234E287V (GAA→GTA) mdtBmultidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (119/115);  total (119/115)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

CCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCA  >  CP009273/2149459‑2149746
                                                                                                                                                |                                                                                                                                               
ccGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACa                                                                                                                                              <  2:612900/148‑1 (MQ=255)
     aaCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCa                                                                                                                                         >  1:3073025/1‑148 (MQ=255)
      aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                        >  2:866141/1‑148 (MQ=255)
      aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                        >  2:2414410/1‑148 (MQ=255)
          ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgtg                                                                                                                                    >  2:1273028/1‑148 (MQ=255)
          ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgtg                                                                                                                                    >  1:2323230/1‑148 (MQ=255)
           cAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtga                                                                                                                                   >  1:2838357/1‑148 (MQ=255)
           cAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtga                                                                                                                                   <  1:2781544/148‑1 (MQ=255)
             gATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatg                                                                                                                                 >  2:2561731/1‑148 (MQ=255)
                 cAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATg                                                                                                                             >  2:3438496/1‑148 (MQ=255)
                   aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGtt                                                                                                                           <  1:450847/148‑1 (MQ=255)
                    tCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                          >  1:3250693/1‑148 (MQ=255)
                    tCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                          >  1:3248188/1‑148 (MQ=255)
                        ccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAgcg                                                                                                                      >  1:1972617/1‑148 (MQ=255)
                          gAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGcc                                                                                                                    <  1:434619/148‑1 (MQ=255)
                          gAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGcc                                                                                                                    <  1:910043/148‑1 (MQ=255)
                           aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                   >  2:1481559/1‑148 (MQ=255)
                           aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                   >  2:2668239/1‑148 (MQ=255)
                             gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                 <  1:1429146/148‑1 (MQ=255)
                               gTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGccc                                                                                                               >  2:2418818/1‑148 (MQ=255)
                                tATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCg                                                                                                              <  2:2260055/148‑1 (MQ=255)
                                   cgaaaGCTAATAATCGCCAACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACAGTAGAGCAAGGTGCATAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTg                                                                                                           >  2:461847/5‑148 (MQ=255)
                                   cGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTg                                                                                                           >  2:2226707/1‑148 (MQ=255)
                                   cGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTg                                                                                                           >  2:3328556/1‑148 (MQ=255)
                                   cGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTg                                                                                                           <  2:2956862/148‑1 (MQ=255)
                                    gCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGc                                                                                                          <  1:3414601/148‑1 (MQ=255)
                                      cAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTa                                                                                                        <  1:520290/148‑1 (MQ=255)
                                      cAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTa                                                                                                        <  1:520989/148‑1 (MQ=255)
                                       agataataaTCGCCTACCAGAAAGGCGCTCCAATTCTACTGCGCGATGTCGCAACTGTAGAGCAAGTTGCAGTAATCAGATGGCTCGGCTCGTGGGAGAACATATTACATTCAATAGTGATGAATGTACAGCGCCAGCCATGTGCTaa                                                                                                       >  2:586210/4‑148 (MQ=255)
                                        gCTAATCATCGCCTACCAGAACGGCGCGCCAAGTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAc                                                                                                      <  1:1736423/148‑1 (MQ=255)
                                          tAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGAAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACAt                                                                                                    <  1:3020602/148‑1 (MQ=255)
                                           aaTCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACAtt                                                                                                   >  2:1610896/1‑148 (MQ=255)
                                            atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                  <  1:1926606/148‑1 (MQ=255)
                                            atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                  >  2:2862823/1‑148 (MQ=255)
                                            atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                  >  2:2645719/1‑148 (MQ=255)
                                            atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                  <  1:151118/148‑1 (MQ=255)
                                             tcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAt                                                                                                 >  2:372175/1‑148 (MQ=255)
                                             tcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAt                                                                                                 >  2:1758983/1‑148 (MQ=255)
                                              catcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtc                                                                                                >  1:3075933/1‑148 (MQ=255)
                                              catcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtc                                                                                                <  2:1815242/148‑1 (MQ=255)
                                               atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtct                                                                                               >  1:867635/1‑148 (MQ=255)
                                                   ccTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAc                                                                                           >  2:316662/1‑148 (MQ=255)
                                                     tACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccg                                                                                         <  1:696493/148‑1 (MQ=255)
                                                        cAGAACGGCGCGCCACTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgccg                                                                                      <  2:1112147/148‑1 (MQ=255)
                                                        cAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGAAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgccg                                                                                      <  2:1533746/148‑1 (MQ=255)
                                                         aGAACGGCGCGCCACTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAAAAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGa                                                                                     <  1:1847952/148‑1 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                    >  1:135938/1‑148 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                    >  1:137687/1‑148 (MQ=255)
                                                          gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                    <  1:2276548/148‑1 (MQ=255)
                                                           aaCGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACa                                                                                   >  2:711769/1‑148 (MQ=255)
                                                            aCGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAg                                                                                  <  1:3424725/148‑1 (MQ=255)
                                                             cGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGc                                                                                 <  1:1018482/148‑1 (MQ=255)
                                                             cGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGc                                                                                 >  1:2156874/1‑148 (MQ=255)
                                                             cGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGc                                                                                 >  1:1589190/1‑148 (MQ=255)
                                                               gcgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAt                                                                               <  1:984516/148‑1 (MQ=255)
                                                               gcgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAt                                                                               <  2:1020226/148‑1 (MQ=255)
                                                                cgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAtt                                                                              >  1:2349155/1‑148 (MQ=255)
                                                                cgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAtt                                                                              >  1:2349204/1‑148 (MQ=255)
                                                                   gcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAGTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCgg                                                                           <  2:406394/148‑1 (MQ=255)
                                                                     cAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCa                                                                         >  1:2905782/1‑148 (MQ=255)
                                                                     cAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCa                                                                         >  1:1154263/1‑148 (MQ=255)
                                                                      aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAg                                                                        <  1:3243243/148‑1 (MQ=255)
                                                                        ttCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAt                                                                      >  1:2909536/1‑148 (MQ=255)
                                                                            tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctg                                                                  >  2:1075109/1‑148 (MQ=255)
                                                                            tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctg                                                                  >  2:735995/1‑148 (MQ=255)
                                                                               gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCca                                                               >  2:1860575/1‑148 (MQ=255)
                                                                                 gCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCcaca                                                             <  2:540241/148‑1 (MQ=255)
                                                                                      gTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCa                                                        <  2:1683016/148‑1 (MQ=255)
                                                                                             cTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGagag                                                 >  2:667858/1‑148 (MQ=255)
                                                                                               gTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTc                                               <  2:312233/148‑1 (MQ=255)
                                                                                               gTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTc                                               <  2:279474/148‑1 (MQ=255)
                                                                                                 agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                             <  1:2668239/148‑1 (MQ=255)
                                                                                                 agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                             <  1:766663/148‑1 (MQ=255)
                                                                                                  gagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGTCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGc                                            >  1:3454874/1‑148 (MQ=255)
                                                                                                  gagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGc                                            <  1:1610896/148‑1 (MQ=255)
                                                                                                       aGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGaaa                                       <  2:3040512/148‑1 (MQ=255)
                                                                                                          tGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCg                                    <  1:494805/148‑1 (MQ=255)
                                                                                                              gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                <  2:3250929/148‑1 (MQ=255)
                                                                                                              gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                <  2:2175316/148‑1 (MQ=255)
                                                                                                              gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                <  1:1088205/148‑1 (MQ=255)
                                                                                                               aaaaCAGCTGGCTCGGCGCGTGGGCGTACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGaa                               >  1:3285391/1‑148 (MQ=255)
                                                                                                                  aCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGt                            <  1:340649/148‑1 (MQ=255)
                                                                                                                   cAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTg                           <  2:2337578/148‑1 (MQ=255)
                                                                                                                      cTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACa                        >  1:1567929/1‑148 (MQ=255)
                                                                                                                      cTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCAGAAATCGGTGAAGGTGACa                        >  1:2090118/1‑148 (MQ=255)
                                                                                                                       tGGCTCGGCGAGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGTAGGTGACAg                       <  2:1217843/148‑1 (MQ=255)
                                                                                                                         gCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                     >  2:15168/1‑148 (MQ=255)
                                                                                                                         gCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                     <  2:2349155/148‑1 (MQ=255)
                                                                                                                            cGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCtt                  <  2:845775/148‑1 (MQ=255)
                                                                                                                             ggCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCGCTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCttt                 <  1:2525801/148‑1 (MQ=255)
                                                                                                                             ggCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCttt                 <  1:2957286/148‑1 (MQ=255)
                                                                                                                              gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTc                <  1:3438496/148‑1 (MQ=255)
                                                                                                                               cgcgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTcc               >  2:2008582/1‑148 (MQ=255)
                                                                                                                                gcgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCg              <  2:3248188/148‑1 (MQ=255)
                                                                                                                                 cgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGa             >  1:368664/1‑148 (MQ=255)
                                                                                                                                 cgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGa             <  2:1568543/148‑1 (MQ=255)
                                                                                                                                          aCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGGATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac    >  2:2340072/1‑148 (MQ=255)
                                                                                                                                            aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca  <  1:2131961/148‑1 (MQ=255)
                                                                                                                                            aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca  <  1:2418753/148‑1 (MQ=255)
                                                                                                                                            aaaGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCAccacca  <  1:2418821/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCA  >  CP009273/2149459‑2149746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑