Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 185,393 A→T 100% F201I (TTC→ATC)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273185,3930AT98.6% 685.0 / ‑0.3 208F201I (TTC→ATC) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (110/95);  total (111/97)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

GTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGA  >  CP009273/185248‑185535
                                                                                                                                                 |                                                                                                                                              
   aGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCa                                                                                                                                           <  1:2010427/148‑1 (MQ=255)
      aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATcc                                                                                                                                        <  2:1982387/148‑1 (MQ=255)
      aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATcc                                                                                                                                        >  2:158115/1‑148 (MQ=255)
          cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAgg                                                                                                                                    <  2:601532/148‑1 (MQ=255)
           gCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGt                                                                                                                                   <  2:2388573/148‑1 (MQ=255)
             aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                 <  2:2498624/148‑1 (MQ=255)
               ccGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCa                                                                                                                               >  2:2994116/1‑148 (MQ=255)
               ccGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCa                                                                                                                               >  1:260262/1‑148 (MQ=255)
                cGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAg                                                                                                                              <  2:831187/148‑1 (MQ=255)
                      cAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                        >  1:1506699/1‑148 (MQ=255)
                      cAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                        >  1:1765610/1‑148 (MQ=255)
                        gTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGt                                                                                                                      >  1:1109409/1‑148 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                     <  1:878347/148‑1 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                     >  2:1069599/1‑148 (MQ=255)
                            ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                  >  1:2042123/1‑148 (MQ=255)
                            ccacAATAGTATGCTCATAGTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                  <  2:77305/148‑1 (MQ=255)
                              acaATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGttt                                                                                                                <  1:2692540/148‑1 (MQ=255)
                                        gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTc                                                                                                      <  1:1555898/148‑1 (MQ=255)
                                         cTCATATTGTGCAGACAAGCTGCGAACTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTAACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCa                                                                                                     >  1:2913189/1‑148 (MQ=255)
                                          tCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                    >  2:1926787/1‑148 (MQ=255)
                                          tCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                    >  1:3383496/1‑148 (MQ=255)
                                           cATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATa                                                                                                   >  2:119293/1‑148 (MQ=255)
                                            atatTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCTTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAg                                                                                                  <  1:316751/148‑1 (MQ=255)
                                               ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgc                                                                                               >  2:1737787/1‑148 (MQ=255)
                                                tgtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                              >  2:969388/1‑148 (MQ=255)
                                                 gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcag                                                                                             <  2:3510283/148‑1 (MQ=255)
                                                  ggCAGACAAGCTGCGCTCTGTGGTGTTTACCGTCCAGCCATCTTTCCTGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagc                                                                                            <  2:3253974/147‑1 (MQ=255)
                                                   gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagca                                                                                           <  2:2101602/148‑1 (MQ=255)
                                                      gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCt                                                                                        <  2:589410/148‑1 (MQ=255)
                                                       aCAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTg                                                                                       >  2:3141508/1‑148 (MQ=255)
                                                         aaGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCg                                                                                     <  1:455827/148‑1 (MQ=255)
                                                          aGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCgg                                                                                    <  1:1485675/148‑1 (MQ=255)
                                                          aGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCgg                                                                                    <  1:3081366/148‑1 (MQ=255)
                                                           gCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGt                                                                                   >  2:2740564/1‑148 (MQ=255)
                                                            cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGtt                                                                                  <  1:2804384/148‑1 (MQ=255)
                                                            cTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGtt                                                                                  <  1:2719415/148‑1 (MQ=255)
                                                              gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttct                                                                                <  1:2281839/148‑1 (MQ=255)
                                                              gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttct                                                                                >  2:424676/1‑148 (MQ=255)
                                                                gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttcttc                                                                              <  1:989449/148‑1 (MQ=255)
                                                                gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttcttc                                                                              <  1:821314/148‑1 (MQ=255)
                                                                 aTCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                             >  2:2208903/1‑148 (MQ=255)
                                                                 aTCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                             >  1:2710194/1‑148 (MQ=255)
                                                                  tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt                                                                            >  2:1464577/1‑148 (MQ=255)
                                                                  tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt                                                                            >  2:133600/1‑148 (MQ=255)
                                                                     ttGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGa                                                                         >  2:563207/1‑148 (MQ=255)
                                                                      tGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGaa                                                                        <  1:685729/148‑1 (MQ=255)
                                                                       ggTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAg                                                                       <  1:1878042/148‑1 (MQ=255)
                                                                        gTTTTTACCGTCCAGCCATATTTCATGGTGCGGATCTCTTTTTTACACGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGAACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGc                                                                      >  2:1064707/1‑148 (MQ=255)
                                                                           tttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc                                                                   >  2:2363593/1‑148 (MQ=255)
                                                                           tttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc                                                                   <  2:729852/148‑1 (MQ=255)
                                                                             tACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGa                                                                 >  1:3134390/1‑148 (MQ=255)
                                                                              cccGTCCAGCCATCTGTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAc                                                                <  2:3298221/147‑1 (MQ=255)
                                                                              aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAc                                                                <  2:966646/148‑1 (MQ=255)
                                                                              aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAc                                                                <  1:121660/148‑1 (MQ=255)
                                                                                cGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCTCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa                                                              <  2:742822/148‑1 (MQ=255)
                                                                                      gCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCg                                                        >  1:1585602/1‑148 (MQ=255)
                                                                                      gCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCg                                                        <  2:2820756/148‑1 (MQ=255)
                                                                                       ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgt                                                       >  1:295704/1‑148 (MQ=255)
                                                                                        cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg                                                      <  2:2233389/148‑1 (MQ=255)
                                                                                        cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg                                                      >  1:2510167/1‑148 (MQ=255)
                                                                                        cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg                                                      >  1:1023733/1‑148 (MQ=255)
                                                                                         aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                     >  2:974066/1‑148 (MQ=255)
                                                                                          tCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                    >  1:2523147/1‑148 (MQ=255)
                                                                                           cTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                   <  1:1685878/148‑1 (MQ=255)
                                                                                           cTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                   >  2:2868933/1‑148 (MQ=255)
                                                                                               cATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCaa                                               <  1:3511599/148‑1 (MQ=255)
                                                                                                  ggTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCCTAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAatat                                            >  2:3297685/1‑148 (MQ=255)
                                                                                                    tGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                          >  2:3491765/1‑148 (MQ=255)
                                                                                                    tGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                          >  2:301750/1‑148 (MQ=255)
                                                                                                     gCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCa                                         <  1:2276224/148‑1 (MQ=255)
                                                                                                        gATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacga                                      <  1:3024608/148‑1 (MQ=255)
                                                                                                         aTCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaa                                     <  1:1069558/148‑1 (MQ=255)
                                                                                                          tctcTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCCTAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                    >  2:3318959/1‑148 (MQ=255)
                                                                                                              ttttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgacg                                <  2:3236569/148‑1 (MQ=255)
                                                                                                               tttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                               >  1:3246799/1‑148 (MQ=255)
                                                                                                                ttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGga                              >  2:665638/1‑148 (MQ=255)
                                                                                                                 tttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgag                             >  2:689917/1‑148 (MQ=255)
                                                                                                                 tttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgag                             >  2:2825588/1‑148 (MQ=255)
                                                                                                                     cccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                         <  1:3491765/148‑1 (MQ=255)
                                                                                                                     cccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                         <  1:2562897/148‑1 (MQ=255)
                                                                                                                      ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                        >  1:1083730/1‑148 (MQ=255)
                                                                                                                      ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                        <  2:1674561/148‑1 (MQ=255)
                                                                                                                      ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                        <  2:872559/148‑1 (MQ=255)
                                                                                                                       cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCACTAGTCACGAACGACGGAGAAGCCt                       >  2:2624070/1‑148 (MQ=255)
                                                                                                                        gcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCtt                      >  2:100426/1‑148 (MQ=255)
                                                                                                                            tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGc                  >  1:776724/1‑148 (MQ=255)
                                                                                                                            tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGc                  <  2:1772268/148‑1 (MQ=255)
                                                                                                                               ccATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTc               >  2:3064386/1‑148 (MQ=255)
                                                                                                                                 aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGa             >  2:1489956/1‑148 (MQ=255)
                                                                                                                                 aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGa             <  2:731540/148‑1 (MQ=255)
                                                                                                                                     gCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaaa         <  1:2450930/148‑1 (MQ=255)
                                                                                                                                      cTCGATGGTGTTCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAt        >  2:1789469/1‑148 (MQ=255)
                                                                                                                                        cGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAttt      >  1:1562392/1‑148 (MQ=255)
                                                                                                                                          aTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt    >  1:1794172/1‑148 (MQ=255)
                                                                                                                                          aTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt    >  1:1794213/1‑148 (MQ=255)
                                                                                                                                          aTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt    >  1:1300206/1‑148 (MQ=255)
                                                                                                                                           tGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTg   >  2:658653/1‑148 (MQ=255)
                                                                                                                                           tGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTACTGCTTCGACAAATTTCTg   >  2:295205/1‑148 (MQ=255)
                                                                                                                                            ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGa  >  1:1674280/1‑148 (MQ=255)
                                                                                                                                            ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGa  >  2:2900411/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGA  >  CP009273/185248‑185535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑