Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,194,695 T→A 100% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,194,6950TA100.0% 548.5 / ‑6.7 167L313Q (CTA→CAA) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (70/96);  total (70/97)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

AACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGA  >  CP009273/2194550‑2194839
                                                                                                                                                 |                                                                                                                                                
aaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCCTCCGCAAGTCATCCGCACCTTGCAAc                                                                                                                                                <  2:942962/148‑1 (MQ=255)
  ccACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtg                                                                                                                                              >  2:2780486/1‑148 (MQ=255)
    aCTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggt                                                                                                                                            <  2:1100864/148‑1 (MQ=255)
      ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtgg                                                                                                                                          >  2:3216406/1‑148 (MQ=255)
      ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtgg                                                                                                                                          <  1:2780486/148‑1 (MQ=255)
       tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGc                                                                                                                                         >  2:207900/1‑148 (MQ=255)
         ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTg                                                                                                                                       <  1:1362596/148‑1 (MQ=255)
          gcgaCGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                      <  2:2381138/145‑1 (MQ=255)
           cTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGAt                                                                                                                                     <  1:2666162/148‑1 (MQ=255)
              cgcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                  >  1:2981846/1‑148 (MQ=255)
              cgcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                  >  1:1876065/1‑148 (MQ=255)
              cgcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                  >  1:1328949/1‑148 (MQ=255)
                cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaaa                                                                                                                                >  2:149454/1‑148 (MQ=255)
                cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaaa                                                                                                                                >  2:295085/1‑148 (MQ=255)
                   ccTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaa                                                                                                                             >  1:177238/1‑148 (MQ=255)
                   ccTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaa                                                                                                                             <  2:2951854/148‑1 (MQ=255)
                     tGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaaac                                                                                                                           <  1:2416995/148‑1 (MQ=255)
                          cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGc                                                                                                                      >  2:2269444/1‑148 (MQ=255)
                           gccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGcc                                                                                                                     <  1:2943810/148‑1 (MQ=255)
                            cccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                    >  1:32849/1‑148 (MQ=255)
                             ccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTg                                                                                                                   <  2:2217346/148‑1 (MQ=255)
                               ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                 >  2:1866065/1‑148 (MQ=255)
                                tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCaa                                                                                                                <  2:2046498/148‑1 (MQ=255)
                                tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCaa                                                                                                                >  1:1607630/1‑148 (MQ=255)
                                  tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                              <  1:2289852/148‑1 (MQ=255)
                                  tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                              <  1:524720/148‑1 (MQ=255)
                                  tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                              <  1:791339/148‑1 (MQ=255)
                                  tGCGCGAACAAGGACTGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                              <  1:1055157/148‑1 (MQ=255)
                                     gcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                           <  1:3007633/148‑1 (MQ=255)
                                     gcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                           <  2:2650495/148‑1 (MQ=255)
                                           aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaa                                                                                                     >  1:1471204/1‑148 (MQ=255)
                                            aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaaa                                                                                                    >  1:2464771/1‑148 (MQ=255)
                                             ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                   >  2:1101025/1‑148 (MQ=255)
                                             ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                   >  2:3506396/1‑148 (MQ=255)
                                                 tGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCa                                                                                               <  2:3491598/148‑1 (MQ=255)
                                                        cATTACGCGACTCGCGTTGTATGCCGATAAAGTAGATTGCGGCAGTCTGATGGTACAAATTAACCATCCGCAAGTCATCCGCAAATTGAAACTTGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAAc                                                                                        <  1:2708232/148‑1 (MQ=255)
                                                          ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                      >  1:25752/1‑148 (MQ=255)
                                                          ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                      <  2:2740221/148‑1 (MQ=255)
                                                          ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                      >  2:1309720/1‑148 (MQ=255)
                                                           tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTc                                                                                     >  2:3416120/1‑148 (MQ=255)
                                                              gcgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                  <  2:1970375/148‑1 (MQ=255)
                                                              gcgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                  <  1:800512/148‑1 (MQ=255)
                                                              gcgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                  <  2:632210/148‑1 (MQ=255)
                                                               cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTccccc                                                                                 <  1:35087/148‑1 (MQ=255)
                                                                gcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCa                                                                                <  1:816083/148‑1 (MQ=255)
                                                                  cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                              <  2:28901/148‑1 (MQ=255)
                                                                  cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                              >  2:345941/1‑148 (MQ=255)
                                                                   tCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGc                                                                             >  2:2141791/1‑148 (MQ=255)
                                                                    cgcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCg                                                                            <  2:1799104/148‑1 (MQ=255)
                                                                        aTGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGc                                                                        >  2:476592/1‑148 (MQ=255)
                                                                         tttATGCCCATATAGAAGATTGCGGCTGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCt                                                                       <  1:3080190/146‑1 (MQ=255)
                                                                         tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCt                                                                       >  2:3261680/1‑148 (MQ=255)
                                                                         tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCt                                                                       <  1:1894922/148‑1 (MQ=255)
                                                                          gTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCCTAAAAACTTCCCCCATGCGACGCTc                                                                      >  2:3304844/1‑148 (MQ=255)
                                                                          gTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTc                                                                      >  2:1060591/1‑148 (MQ=255)
                                                                              gCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgccg                                                                  >  1:1830518/1‑148 (MQ=255)
                                                                                  aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                              <  1:3122070/148‑1 (MQ=255)
                                                                                    aaaGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                            >  2:1409518/1‑148 (MQ=255)
                                                                                    aaaGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                            <  2:1649251/148‑1 (MQ=255)
                                                                                    aaaGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                            <  2:1651879/148‑1 (MQ=255)
                                                                                     aagaagATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGc                                                           >  2:215540/1‑148 (MQ=255)
                                                                                          gaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                      <  2:585147/148‑1 (MQ=255)
                                                                                          gaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                      <  2:585148/148‑1 (MQ=255)
                                                                                          gaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAc                                                      >  1:1213406/1‑148 (MQ=255)
                                                                                           aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAct                                                     >  1:899596/1‑148 (MQ=255)
                                                                                            ttGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActg                                                    <  1:1441028/148‑1 (MQ=255)
                                                                                             tGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                   <  2:1167845/148‑1 (MQ=255)
                                                                                                  cAGTCTGCCGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCg                                              <  1:942855/148‑1 (MQ=255)
                                                                                                   aGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGt                                             <  2:2256794/148‑1 (MQ=255)
                                                                                                     tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTACCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTa                                           >  2:2381328/1‑148 (MQ=255)
                                                                                                        gctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGGTAACa                                        >  2:2701854/1‑148 (MQ=255)
                                                                                                          tgttACAAATTAACCATCCGCTAGTCAGACTCAAATTTCTACTTTTGGCTGATAATAACAAACCCAGCCTTTAACGTGTTTCTAAATAACATATAATCTTCCGCCATGCTACTCTCGCCGCACTGGCAGAACTGCTGTCGTTAAAAGa                                      <  2:1306399/145‑1 (MQ=255)
                                                                                                          tgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGa                                      <  1:3313935/148‑1 (MQ=255)
                                                                                                               cAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAaccac                                 <  2:1921216/148‑1 (MQ=255)
                                                                                                                 aaTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAccacca                               >  1:2252256/1‑148 (MQ=255)
                                                                                                                  aTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAg                              >  1:2005817/1‑148 (MQ=255)
                                                                                                                  aTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAg                              >  1:234352/1‑148 (MQ=255)
                                                                                                                  aTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAg                              >  2:2635244/1‑148 (MQ=255)
                                                                                                                    tAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcc                            <  2:177238/148‑1 (MQ=255)
                                                                                                                        cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgcc                        <  1:3387888/148‑1 (MQ=255)
                                                                                                                        cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgcc                        <  2:1774526/148‑1 (MQ=255)
                                                                                                                         aTCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                       <  1:3216406/148‑1 (MQ=255)
                                                                                                                         aTCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                       >  1:2105979/1‑148 (MQ=255)
                                                                                                                           ccGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTg                     >  2:1777047/1‑148 (MQ=255)
                                                                                                                            cGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTgg                    >  1:896041/1‑148 (MQ=255)
                                                                                                                                aGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTAt                <  2:1822179/148‑1 (MQ=255)
                                                                                                                                 gTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc               <  2:2218981/148‑1 (MQ=255)
                                                                                                                                   cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCa             <  1:207900/148‑1 (MQ=255)
                                                                                                                                    agccGCACCTTGCAACTGGTGGCTGATAAAAACCAACCCAGCCTGCAACGTGTAGCTAAATACCATACAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGGTATCCaa            <  2:1782848/146‑1 (MQ=255)
                                                                                                                                     tCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAat           >  2:1585346/1‑148 (MQ=255)
                                                                                                                                       cGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatca         <  2:2981846/148‑1 (MQ=255)
                                                                                                                                       cGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatca         <  2:1328949/148‑1 (MQ=255)
                                                                                                                                        gCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTACATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatcat        <  2:3437793/148‑1 (MQ=255)
                                                                                                                                        gCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatcat        >  1:2497130/1‑148 (MQ=255)
                                                                                                                                        gCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACGTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatcat        <  2:1473306/148‑1 (MQ=255)
                                                                                                                                         cACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatcatc       >  1:1861386/1‑148 (MQ=255)
                                                                                                                                          aCCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCNTGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCg      >  1:1784164/1‑148 (MQ=255)
                                                                                                                                            cTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGaa    >  1:1550865/1‑148 (MQ=255)
                                                                                                                                             ttGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAg   <  2:1156857/148‑1 (MQ=255)
                                                                                                                                             ttGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAg   >  1:2937567/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGA  >  CP009273/2194550‑2194839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑