Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,241,222 T→G 100% I264L (ATT→CTT)  lysP ← lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,241,2220TG99.1% 862.1 / ‑3.7 210I264L (ATT→CTT) lysPlysine transporter
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (92/116);  total (93/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGA  >  CP009273/2241077‑2241365
                                                                                                                                                 |                                                                                                                                               
  cGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATc                                                                                                                                             >  2:1588368/1‑148 (MQ=255)
       aCAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCt                                                                                                                                        >  1:2421151/1‑148 (MQ=255)
        cAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTg                                                                                                                                       >  2:1109735/1‑148 (MQ=255)
         aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGa                                                                                                                                      <  1:552494/148‑1 (MQ=255)
          gCACCGCCGTCAGAAGAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                     <  2:2156577/148‑1 (MQ=255)
           cACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                    >  2:306014/1‑148 (MQ=255)
            aCCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATaa                                                                                                                                   <  1:2787393/148‑1 (MQ=255)
              cgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATc                                                                                                                                 <  1:1024900/148‑1 (MQ=255)
               gccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCa                                                                                                                                <  1:1386837/148‑1 (MQ=255)
                 cgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAgg                                                                                                                              <  2:1656736/148‑1 (MQ=255)
                 cgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAgg                                                                                                                              <  1:2328309/148‑1 (MQ=255)
                  gTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGa                                                                                                                             <  1:855555/148‑1 (MQ=255)
                  gTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGa                                                                                                                             <  1:2897045/148‑1 (MQ=255)
                     aGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcg                                                                                                                          <  2:107244/148‑1 (MQ=255)
                      gAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                         >  2:1270800/1‑148 (MQ=255)
                      gAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                         >  1:1899148/1‑148 (MQ=255)
                        aTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGa                                                                                                                       <  2:2238405/148‑1 (MQ=255)
                         tAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGaa                                                                                                                      >  1:2388748/1‑148 (MQ=255)
                          aaCTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAGCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAac                                                                                                                     <  2:1910319/148‑1 (MQ=255)
                           aCTGCGTTAATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCATTGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCATCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAaca                                                                                                                    <  2:3009911/148‑1 (MQ=255)
                            cTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAacac                                                                                                                   >  2:2119398/1‑148 (MQ=255)
                            cTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAacac                                                                                                                   >  1:695281/1‑148 (MQ=255)
                             tGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAcaca                                                                                                                  >  2:1538387/1‑148 (MQ=255)
                              gCGTTCATCACCGCCGCCGCAGAGATCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACAt                                                                                                                 <  1:1939631/148‑1 (MQ=255)
                                     tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaa                                                                                                          >  2:517075/1‑148 (MQ=255)
                                      caCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                         >  1:1140905/1‑148 (MQ=255)
                                           ccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAt                                                                                                    <  2:3246884/148‑1 (MQ=255)
                                            cgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAtt                                                                                                   <  1:358068/148‑1 (MQ=255)
                                                gcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGcc                                                                                               <  1:2508594/148‑1 (MQ=255)
                                                 cAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCa                                                                                              >  1:1584605/1‑148 (MQ=255)
                                                   gagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGa                                                                                            <  2:3394308/148‑1 (MQ=255)
                                                   gagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGa                                                                                            <  2:1220839/148‑1 (MQ=255)
                                                    agagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGaa                                                                                           >  2:85339/1‑148 (MQ=255)
                                                     gagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAac                                                                                          <  2:3090660/148‑1 (MQ=255)
                                                        cAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACAcc                                                                                       <  1:3109135/148‑1 (MQ=255)
                                                        cAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACAcc                                                                                       <  1:3366368/148‑1 (MQ=255)
                                                          gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTg                                                                                     >  2:2332636/1‑148 (MQ=255)
                                                          gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTg                                                                                     <  1:1646355/148‑1 (MQ=255)
                                                           aCCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                    >  2:802071/1‑148 (MQ=255)
                                                           aCCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                    >  2:1013154/1‑148 (MQ=255)
                                                            cccGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGAc                                                                                   >  2:2922324/1‑148 (MQ=255)
                                                               gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                <  1:3377862/148‑1 (MQ=255)
                                                               gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                <  1:2942708/148‑1 (MQ=255)
                                                               gcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                <  1:1189788/148‑1 (MQ=255)
                                                                cgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAc                                                                               >  1:1581275/1‑148 (MQ=255)
                                                                      ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgcg                                                                         <  2:382799/148‑1 (MQ=255)
                                                                      ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgcg                                                                         >  2:897206/1‑148 (MQ=255)
                                                                      ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgcg                                                                         <  1:2873918/148‑1 (MQ=255)
                                                                       gAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGt                                                                        >  1:479631/1‑148 (MQ=255)
                                                                        aaCACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTg                                                                       <  2:1193661/148‑1 (MQ=255)
                                                                         acacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                      <  1:1845242/148‑1 (MQ=255)
                                                                          cacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGa                                                                     <  1:2974997/148‑1 (MQ=255)
                                                                           acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGaa                                                                    <  1:627234/148‑1 (MQ=255)
                                                                             cAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATg                                                                  <  1:1700526/148‑1 (MQ=255)
                                                                               gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtt                                                                >  1:2232117/1‑148 (MQ=255)
                                                                                ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                               >  1:47709/1‑148 (MQ=255)
                                                                                ggTGAACGCACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtgt                                                               >  2:1091159/1‑146 (MQ=255)
                                                                                     aCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGc                                                          <  2:3173474/148‑1 (MQ=255)
                                                                                      cGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGcc                                                         >  2:1329557/1‑148 (MQ=255)
                                                                                        gACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCgg                                                       >  2:2829040/1‑148 (MQ=255)
                                                                                           tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                    >  2:42065/1‑148 (MQ=255)
                                                                                           tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                    >  2:2520668/1‑148 (MQ=255)
                                                                                           tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                    >  1:213029/1‑148 (MQ=255)
                                                                                           tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                    <  2:1798324/148‑1 (MQ=255)
                                                                                            aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                   <  1:2255603/148‑1 (MQ=255)
                                                                                            aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                   <  1:1091208/148‑1 (MQ=255)
                                                                                             aCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCtt                                                  <  1:1743037/148‑1 (MQ=255)
                                                                                             aCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCtt                                                  >  1:1866117/1‑148 (MQ=255)
                                                                                              cGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTc                                                 >  1:1082432/1‑148 (MQ=255)
                                                                                               gCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCg                                                <  2:256039/148‑1 (MQ=255)
                                                                                                   aTGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACt                                            <  1:2309459/148‑1 (MQ=255)
                                                                                                     gTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCg                                          >  2:3283052/1‑148 (MQ=255)
                                                                                                      tCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGc                                         >  2:1473029/1‑148 (MQ=255)
                                                                                                          tAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgc                                     <  1:306014/148‑1 (MQ=255)
                                                                                                           aaCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgca                                    <  1:2695596/148‑1 (MQ=255)
                                                                                                            aCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcag                                   >  2:1079362/1‑148 (MQ=255)
                                                                                                             cATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagc                                  >  2:2834429/1‑148 (MQ=255)
                                                                                                              aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagca                                 <  2:2030715/148‑1 (MQ=255)
                                                                                                              aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagca                                 >  2:344603/1‑148 (MQ=255)
                                                                                                                  ttACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                             >  2:2436118/1‑148 (MQ=255)
                                                                                                                      gcagcaGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGAt                         <  2:2406729/148‑1 (MQ=255)
                                                                                                                         gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAg                      >  1:2575108/1‑148 (MQ=255)
                                                                                                                         gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCGTCGGACTCGCCTGCAGCAATACCGATCAg                      <  2:1690660/148‑1 (MQ=255)
                                                                                                                          cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                     >  2:1086135/1‑148 (MQ=255)
                                                                                                                          cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                     >  1:3503076/1‑148 (MQ=255)
                                                                                                                             gCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACCACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCg                  >  2:2721625/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 >  2:2102360/1‑148 (MQ=255)
                                                                                                                              cTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                 <  2:65419/148‑1 (MQ=255)
                                                                                                                               tCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGt                >  1:3233346/1‑148 (MQ=255)
                                                                                                                                cGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGtt               <  2:34133/148‑1 (MQ=255)
                                                                                                                                  gATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTcc             <  1:2681503/148‑1 (MQ=255)
                                                                                                                                   aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTccc            <  1:2922324/148‑1 (MQ=255)
                                                                                                                                   aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTccc            <  1:633019/148‑1 (MQ=255)
                                                                                                                                       gtgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGa        >  1:875155/1‑148 (MQ=255)
                                                                                                                                         gtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa      >  1:1445753/1‑148 (MQ=255)
                                                                                                                                           aCGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACCGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAaga    <  2:3315957/148‑1 (MQ=255)
                                                                                                                                            cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAagag   >  2:378117/1‑148 (MQ=255)
                                                                                                                                            cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAagag   >  2:378536/1‑148 (MQ=255)
                                                                                                                                             ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga  <  1:2034513/148‑1 (MQ=255)
                                                                                                                                             ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga  >  2:2997213/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGA  >  CP009273/2241077‑2241365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑