Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,245,476 G→A 94.0% A100T (GCC→ACC)  yeiI → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,245,4760GA94.0% 904.2 / 37.4 285A100T (GCC→ACC) yeiIputative kinase
Reads supporting (aligned to +/- strand):  ref base G (11/6);  new base A (130/138);  total (141/144)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 285 total aligned reads displayed.

TTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATC  >  CP009273/2245331‑2245621
                                                                                                                                                 |                                                                                                                                                 
     ttCTCACCGAGCAGGAATACTGCGGAGTGGTGGGGACAATCAATATGGATATTCGCGGGAGGGCGGATATCCGTTACCCGCACACGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACaa                                                                                                                                            >  2:1784982/1‑148 (MQ=255)
      tctcACCGAGCAGGAATAGTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCGCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGTCGCAACTTCACCCACAAt                                                                                                                                           <  2:879002/148‑1 (MQ=255)
      tctcACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAAt                                                                                                                                           <  2:549948/148‑1 (MQ=255)
        tcACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCt                                                                                                                                         <  2:3177108/148‑1 (MQ=255)
         cACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTg                                                                                                                                        <  1:719434/148‑1 (MQ=255)
           ccGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGgc                                                                                                                                      >  1:2748678/1‑148 (MQ=255)
           ccGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGgc                                                                                                                                      >  1:2905763/1‑148 (MQ=255)
                cAGGAATACTGCGTAGTGGTGGGTACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATGGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                 <  2:1056085/148‑1 (MQ=255)
                cAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCTGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                 <  2:3279855/148‑1 (MQ=255)
                cAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                 <  2:145520/148‑1 (MQ=255)
                cAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                 <  2:154681/148‑1 (MQ=255)
                cAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                 <  2:3067392/148‑1 (MQ=255)
                 aGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATAGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGtt                                                                                                                                <  2:1492677/148‑1 (MQ=255)
                   gAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAg                                                                                                                              >  1:2602284/1‑148 (MQ=255)
                   gAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAg                                                                                                                              >  1:3048289/1‑148 (MQ=255)
                    aaTACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAgg                                                                                                                             <  2:251711/148‑1 (MQ=255)
                       aCTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCg                                                                                                                          <  2:523890/148‑1 (MQ=255)
                         tGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTg                                                                                                                        >  1:514954/1‑148 (MQ=255)
                          gCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGa                                                                                                                       <  1:2241349/148‑1 (MQ=255)
                            gTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACg                                                                                                                     <  1:3171169/148‑1 (MQ=255)
                              aGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTc                                                                                                                   >  2:2878682/1‑148 (MQ=255)
                                  gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCAtt                                                                                                               <  1:1167274/148‑1 (MQ=255)
                                  gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCAtt                                                                                                               <  2:1717658/148‑1 (MQ=255)
                                     gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGc                                                                                                            >  1:2383410/1‑148 (MQ=255)
                                     gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGc                                                                                                            <  2:31391/148‑1 (MQ=255)
                                     gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGc                                                                                                            <  2:31435/148‑1 (MQ=255)
                                      ggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCt                                                                                                           <  2:284841/148‑1 (MQ=255)
                                      ggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCt                                                                                                           <  2:3255726/148‑1 (MQ=255)
                                        aCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCttt                                                                                                         >  2:1765855/1‑148 (MQ=255)
                                        aCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCttt                                                                                                         >  2:3503090/1‑148 (MQ=255)
                                            tcaatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGt                                                                                                     >  1:209321/1‑148 (MQ=255)
                                              aatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGa                                                                                                   >  2:813289/1‑148 (MQ=255)
                                              aatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGa                                                                                                   <  2:1111550/148‑1 (MQ=255)
                                               atatGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGAt                                                                                                  <  1:1556076/148‑1 (MQ=255)
                                               atatGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGAt                                                                                                  >  1:2940743/1‑148 (MQ=255)
                                                 atGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTg                                                                                                >  2:1537778/1‑148 (MQ=255)
                                                   ggATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGc                                                                                              <  2:1170646/148‑1 (MQ=255)
                                                       atTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATg                                                                                          <  1:2426646/148‑1 (MQ=255)
                                                       atTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATg                                                                                          >  2:3403864/1‑148 (MQ=255)
                                                         tCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGAc                                                                                        <  2:103671/148‑1 (MQ=255)
                                                         tCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCGGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGAc                                                                                        >  2:2295709/1‑148 (MQ=255)
                                                            cgGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACttt                                                                                     <  1:1991651/148‑1 (MQ=255)
                                                              ggATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTa                                                                                   >  2:1878480/1‑148 (MQ=255)
                                                               gATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTAt                                                                                  <  2:332728/148‑1 (MQ=255)
                                                                 tGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATgg                                                                                <  1:842763/148‑1 (MQ=255)
                                                                    cGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGa                                                                             >  1:14795/1‑148 (MQ=255)
                                                                      gATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaaa                                                                           <  2:2618510/148‑1 (MQ=255)
                                                                      gATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaaa                                                                           >  2:2777388/1‑148 (MQ=255)
                                                                      gATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaaa                                                                           <  2:1298913/148‑1 (MQ=255)
                                                                      gATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaaa                                                                           >  2:564202/1‑148 (MQ=255)
                                                                       atatCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAAt                                                                          >  1:3457339/1‑148 (MQ=255)
                                                                         atCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGc                                                                        <  2:2383410/148‑1 (MQ=255)
                                                                           ccGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTc                                                                      >  1:2520353/1‑148 (MQ=255)
                                                                            cGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTcc                                                                     <  1:1671155/148‑1 (MQ=255)
                                                                                 cccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaa                                                                >  1:1023244/1‑148 (MQ=255)
                                                                                 cccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaa                                                                >  1:991712/1‑148 (MQ=255)
                                                                                  ccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACACTCTGGCGCTGTTCGGCCGTGACGTCCAGTTGCTTTCAGTGATTGGCGATGACTGTTATGGCGAAATGCTCCTGgaag                                                               >  2:738831/1‑148 (MQ=255)
                                                                                  ccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaag                                                               >  1:2299528/1‑148 (MQ=255)
                                                                                  ccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaag                                                               >  1:1189531/1‑148 (MQ=255)
                                                                                   cGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaaga                                                              <  1:1007086/148‑1 (MQ=255)
                                                                                     cAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGaaa                                                            <  1:1738813/148‑1 (MQ=255)
                                                                                       aTCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcg                                                          >  1:1056813/1‑148 (MQ=255)
                                                                                       aTCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcg                                                          >  1:1667926/1‑148 (MQ=255)
                                                                                         cGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcgcg                                                        >  2:2257083/1‑148 (MQ=255)
                                                                                           gCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGcc                                                      <  1:2965459/148‑1 (MQ=255)
                                                                                           gCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGcc                                                      >  2:1205181/1‑148 (MQ=255)
                                                                                              tctcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTg                                                   <  1:2149340/148‑1 (MQ=255)
                                                                                               ctcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGc                                                  <  1:866487/148‑1 (MQ=255)
                                                                                                tcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                 >  1:442795/1‑148 (MQ=255)
                                                                                                 cATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCAGTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGACACGCGCCGTGCCg                                                >  2:2075869/1‑148 (MQ=255)
                                                                                                        gTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGaa                                         <  2:974291/148‑1 (MQ=255)
                                                                                                        gTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGaa                                         <  2:1161991/148‑1 (MQ=255)
                                                                                                        gTACAATTCATGGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGaa                                         <  2:2179732/148‑1 (MQ=255)
                                                                                                         tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAAt                                        <  1:935457/148‑1 (MQ=255)
                                                                                                           cAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGt                                      >  1:193767/1‑148 (MQ=255)
                                                                                                           cAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGt                                      <  1:3093247/148‑1 (MQ=255)
                                                                                                            aaTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtc                                     <  1:1343932/148‑1 (MQ=255)
                                                                                                             aTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtct                                    >  1:1260392/1‑148 (MQ=255)
                                                                                                             aTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtct                                    <  1:329501/148‑1 (MQ=255)
                                                                                                             aTTCATAGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAAACTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtct                                    >  2:1968707/1‑148 (MQ=255)
                                                                                                                  ttGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGc                               >  2:2534731/1‑148 (MQ=255)
                                                                                                                     cTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGc                            >  1:278084/1‑148 (MQ=255)
                                                                                                                     cTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGc                            >  1:277988/1‑148 (MQ=255)
                                                                                                                       cAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgt                          <  2:2836895/148‑1 (MQ=255)
                                                                                                                          cgggcggCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttcg                       <  1:1890136/148‑1 (MQ=255)
                                                                                                                          cgggcggCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttcg                       <  1:561203/148‑1 (MQ=255)
                                                                                                                            ggcggcGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttcgtt                     <  2:2619262/148‑1 (MQ=255)
                                                                                                                             gcggcgTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGttt                    <  1:61838/148‑1 (MQ=255)
                                                                                                                                gcgTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCa                 <  1:289757/148‑1 (MQ=255)
                                                                                                                                  gTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGANTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg               <  1:1784936/148‑1 (MQ=255)
                                                                                                                                    gggACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGt             >  1:775907/1‑148 (MQ=255)
                                                                                                                                     ggACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTc            >  1:2632862/1‑148 (MQ=255)
                                                                                                                                     ggACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTc            >  2:691616/1‑148 (MQ=255)
                                                                                                                                     ggACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTc            >  2:906789/1‑148 (MQ=255)
                                                                                                                                       aCGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCaa          >  1:555675/1‑148 (MQ=255)
                                                                                                                                         gCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAg        <  2:2062218/148‑1 (MQ=255)
                                                                                                                                         gCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAg        <  2:2062054/148‑1 (MQ=255)
                                                                                                                                            aCATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGcac     <  1:3376911/148‑1 (MQ=255)
                                                                                                                                              aTCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACAt   <  2:2748678/148‑1 (MQ=255)
                                                                                                                                              aTCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACAt   <  1:2914391/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATC  >  CP009273/2245331‑2245621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑