Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,287,829 C→A 100% S185S (TCG→TCT)  ccmF ← heme lyase, CcmF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,287,8290CA98.8% 520.7 / ‑1.8 165S185S (TCG→TCT) ccmFheme lyase, CcmF subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (82/79);  total (85/80)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 170 total aligned reads displayed.

TTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACA  >  CP009273/2287685‑2287971
                                                                                                                                                |                                                                                                                                              
ttCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                             >  1:1235719/1‑148 (MQ=255)
ttCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                             >  1:2079740/1‑148 (MQ=255)
    tAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAc                                                                                                                                         <  2:2527433/148‑1 (MQ=255)
     aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                        >  1:1056744/1‑148 (MQ=255)
     aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                        >  2:1402208/1‑148 (MQ=255)
      aTAGGCCCATGCGTAACCGAGCACGATGCCGAGCTTCAGGAAGTTCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGATGCAATGGCAAAAGCAAACGCCACAGAGAAAccc                                                                                                                                       <  2:2144964/148‑1 (MQ=255)
          gCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGt                                                                                                                                   >  1:1936267/1‑148 (MQ=255)
             cATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAcc                                                                                                                                >  1:57483/1‑148 (MQ=255)
              aTGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAccc                                                                                                                               >  1:3307521/1‑148 (MQ=255)
              aTGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAccc                                                                                                                               >  1:1570450/1‑148 (MQ=255)
                gCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCa                                                                                                                             >  1:1041304/1‑148 (MQ=255)
                    aaCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCAtata                                                                                                                         >  2:2364649/1‑148 (MQ=255)
                    aaCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCAtata                                                                                                                         >  1:2302434/1‑148 (MQ=255)
                       cGAGCACTATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGATTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGAAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAg                                                                                                                      <  1:2756093/148‑1 (MQ=255)
                         aGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCa                                                                                                                    >  1:2450227/1‑148 (MQ=255)
                          gCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                   >  1:2700948/1‑148 (MQ=255)
                          gCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCag                                                                                                                   >  2:3007461/1‑148 (MQ=255)
                             cGATGCCGAGCTTCTGTAATATCCACGGTGCCAGCTTCCACGGAGGATTAAAACGCGATTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGAAATGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAgg                                                                                                                <  1:2358255/148‑1 (MQ=255)
                              gttGCCGTGAGTCAGTAAGTTCACCGTTGCCAGCGTCCAGGTAAGATTAATCCGCGCATCAGTGCTGTCCAGACGCCCGATCAGAATTGATGCTTTGGCAAAAGAATACGCCACTGATAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                               <  1:2187960/146‑1 (MQ=255)
                                tGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCgg                                                                                                             >  2:1257489/1‑148 (MQ=255)
                                tGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCgg                                                                                                             <  1:2997250/148‑1 (MQ=255)
                                  ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                           >  1:2264404/1‑148 (MQ=255)
                                   cGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATg                                                                                                          <  1:2605529/148‑1 (MQ=255)
                                     aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGa                                                                                                        >  1:2498991/1‑148 (MQ=255)
                                      gCGTCAGTAAGATCCACGCTGCCAGCGTACACGGACGATTAAAACGCTAATAAGTGCTGTCCAGACGCCCGCTCAGCAATGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGaa                                                                                                       <  2:2921047/148‑1 (MQ=255)
                                        gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACTAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGa                                                                                                     <  2:103477/148‑1 (MQ=255)
                                        gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGa                                                                                                     <  2:1879361/148‑1 (MQ=255)
                                         tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                    >  2:3398241/1‑148 (MQ=255)
                                         tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                    >  2:915453/1‑148 (MQ=255)
                                            ggAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                                                                                                 >  1:3018858/1‑148 (MQ=255)
                                              aaGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGcc                                                                                               >  1:1253402/1‑148 (MQ=255)
                                              aaGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGcc                                                                                               >  1:869651/1‑148 (MQ=255)
                                              aaGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGcc                                                                                               >  1:3087761/1‑148 (MQ=255)
                                               aGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGccc                                                                                              <  1:236526/148‑1 (MQ=255)
                                                    cACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATACAGCAGAGGCGGATGGAAGATCAGCCCCGGAt                                                                                         >  1:1800341/1‑148 (MQ=255)
                                                     aCGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATc                                                                                        <  1:1732416/148‑1 (MQ=255)
                                                      cGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATcc                                                                                       <  2:2393076/148‑1 (MQ=255)
                                                        cTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAACCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTg                                                                                     <  1:52911/148‑1 (MQ=255)
                                                          gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa                                                                                   <  1:448748/148‑1 (MQ=255)
                                                                    aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                         >  1:7844/1‑148 (MQ=255)
                                                                    aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                         >  1:7312/1‑148 (MQ=255)
                                                                    aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                         >  2:3464710/1‑148 (MQ=255)
                                                                     cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTa                                                                        <  1:2768599/148‑1 (MQ=255)
                                                                     cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTa                                                                        >  2:720957/1‑148 (MQ=255)
                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                     <  2:3226732/148‑1 (MQ=255)
                                                                        aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                     >  2:3514132/1‑148 (MQ=255)
                                                                         cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                    <  1:1096515/148‑1 (MQ=255)
                                                                         cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                    <  1:1096507/148‑1 (MQ=255)
                                                                           aGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcg                                                                  <  2:1578091/148‑1 (MQ=255)
                                                                             tAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcgcg                                                                <  1:2638024/148‑1 (MQ=255)
                                                                             tAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcgcg                                                                <  1:2824838/148‑1 (MQ=255)
                                                                               aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                              <  1:665633/148‑1 (MQ=255)
                                                                               aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                              >  1:3265537/1‑148 (MQ=255)
                                                                               aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                              >  1:3209565/1‑148 (MQ=255)
                                                                                     gcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCaa                                                        <  2:712978/148‑1 (MQ=255)
                                                                                      cATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                       <  1:2522329/148‑1 (MQ=255)
                                                                                       aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCACTAGCGGGTTAAGATCGCGACCTTCAATc                                                      >  2:2591757/1‑148 (MQ=255)
                                                                                       aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATc                                                      >  2:1986624/1‑148 (MQ=255)
                                                                                        tAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCg                                                     >  1:3159343/1‑148 (MQ=255)
                                                                                         aaGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                    >  1:3212458/1‑148 (MQ=255)
                                                                                           gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                  <  2:7844/148‑1 (MQ=255)
                                                                                                 tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCg                                            <  2:1936267/148‑1 (MQ=255)
                                                                                                  ccAGACGCCCGCACAGCAAAGAAGCAATGGCAAAACCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCgg                                           <  1:300595/148‑1 (MQ=255)
                                                                                                    aGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCa                                         <  1:1182027/148‑1 (MQ=255)
                                                                                                       cGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCACACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACg                                      <  2:3275506/148‑1 (MQ=255)
                                                                                                            gCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCga                                 <  1:875018/148‑1 (MQ=255)
                                                                                                            gCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCga                                 >  2:1794775/1‑148 (MQ=255)
                                                                                                                aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGACGTTCGGCACCGTGCgagaga                             >  1:2904315/1‑148 (MQ=255)
                                                                                                                 gCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGaa                            >  1:286243/1‑148 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagagacc                           >  2:2314160/1‑146 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                           >  1:144177/1‑148 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                           >  1:2602710/1‑148 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                           >  1:3123916/1‑148 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                           >  2:1544048/1‑148 (MQ=255)
                                                                                                                  cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                           >  1:1917476/1‑148 (MQ=255)
                                                                                                                   aaaGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGACGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACg                          >  2:198767/1‑148 (MQ=255)
                                                                                                                    aagaagCCATGGCAAACGCACACGCCACAGCGAAACCCACGTACCCCATATACAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                         <  2:3100198/148‑1 (MQ=255)
                                                                                                                    aagaagCAATTGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCTATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                         <  1:1998938/148‑1 (MQ=255)
                                                                                                                    aagaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                         >  1:3134831/1‑148 (MQ=255)
                                                                                                                         gCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTag                    >  1:2006261/1‑148 (MQ=255)
                                                                                                                         gCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTag                    >  1:2006138/1‑148 (MQ=255)
                                                                                                                          cAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTaga                   <  1:1006118/148‑1 (MQ=255)
                                                                                                                          cAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTaga                   >  2:1392889/1‑148 (MQ=255)
                                                                                                                           aaTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTagag                  <  1:3435579/148‑1 (MQ=255)
                                                                                                                           aaTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTagag                  <  2:3307521/148‑1 (MQ=255)
                                                                                                                             tGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGt                <  2:2813134/148‑1 (MQ=255)
                                                                                                                             tGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGt                >  2:2212137/1‑148 (MQ=255)
                                                                                                                               gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATACAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa              <  2:2155725/148‑1 (MQ=255)
                                                                                                                               gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa              <  2:3033069/148‑1 (MQ=255)
                                                                                                                                cAAAAGCAAACGCCCCTGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCACTAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaaa             >  2:577805/1‑148 (MQ=255)
                                                                                                                                  aaaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAaga           >  1:57871/1‑148 (MQ=255)
                                                                                                                                     gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa        <  2:157780/148‑1 (MQ=255)
                                                                                                                                       aaaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATg      <  2:2498991/148‑1 (MQ=255)
                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa     >  2:174930/1‑148 (MQ=255)
                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa     <  2:2233226/148‑1 (MQ=255)
                                                                                                                                        aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa     <  2:2233179/148‑1 (MQ=255)
                                                                                                                                         aCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGACCGGGTTAGAGGTAAAGAGAATGaa    >  1:2848483/1‑148 (MQ=255)
                                                                                                                                          cGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAAc   <  1:388363/148‑1 (MQ=255)
                                                                                                                                          cGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAAc   <  2:2417782/148‑1 (MQ=255)
                                                                                                                                           gCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACa  <  2:2826117/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACA  >  CP009273/2287685‑2287971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑