Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,358,641 G→T 100% I153I (ATC→ATA)  ais ← putative LPS core heptose(II)‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,358,6410GT98.5% 810.2 / 1.6 253I153I (ATC→ATA) aisputative LPS core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (134/115);  total (135/118)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

TTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTG  >  CP009273/2358497‑2358785
                                                                                                                                                |                                                                                                                                                
ttAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACtata                                                                                                                                               >  1:1272023/1‑148 (MQ=255)
ttAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACtata                                                                                                                                               <  2:2570606/148‑1 (MQ=255)
 tAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTatat                                                                                                                                              <  2:2862349/148‑1 (MQ=255)
  aGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAtt                                                                                                                                             <  2:2014796/148‑1 (MQ=255)
   gTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAttt                                                                                                                                            <  1:1195570/148‑1 (MQ=255)
     ggTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAATTGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAttttt                                                                                                                                          >  2:332118/1‑148 (MQ=255)
      gTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTa                                                                                                                                         <  1:2925529/148‑1 (MQ=255)
       ttAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTAt                                                                                                                                        <  2:1694363/148‑1 (MQ=255)
       ttAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTAt                                                                                                                                        >  1:3050847/1‑148 (MQ=255)
           cGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                    >  1:1370000/1‑148 (MQ=255)
           cGAATTCCCCATCCAGATAAACTTTGCCTTTGTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                    <  2:1975211/148‑1 (MQ=255)
              aTTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGgcgc                                                                                                                                 <  1:492418/148‑1 (MQ=255)
               ttCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCCTCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgct                                                                                                                                <  2:1928340/148‑1 (MQ=255)
               ttCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgct                                                                                                                                <  2:1494435/148‑1 (MQ=255)
               ttCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgct                                                                                                                                >  2:2713908/1‑148 (MQ=255)
               ttCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgct                                                                                                                                <  2:2207821/148‑1 (MQ=255)
                 ccccATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgcttt                                                                                                                              >  2:1679075/1‑148 (MQ=255)
                   ccATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgctttgc                                                                                                                            >  1:3176360/1‑148 (MQ=255)
                      tCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgctttgcttt                                                                                                                         <  1:1808509/148‑1 (MQ=255)
                       ccAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGctttgctttg                                                                                                                        >  2:2606002/1‑148 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      <  1:2254700/148‑1 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      >  1:2351396/1‑148 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      <  2:89589/148‑1 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      <  1:2130067/148‑1 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      >  1:588918/1‑148 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      <  2:726205/148‑1 (MQ=255)
                         aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                      <  1:1490254/148‑1 (MQ=255)
                          gATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTaa                                                                                                                     >  2:3272723/1‑148 (MQ=255)
                          gATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTaa                                                                                                                     >  1:1526123/1‑148 (MQ=255)
                           aTAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                    <  1:895488/148‑1 (MQ=255)
                           aTAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                    >  2:1644988/1‑148 (MQ=255)
                           aTAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                    >  1:2970553/1‑148 (MQ=255)
                           aTAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                    >  1:2809175/1‑148 (MQ=255)
                            tAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGt                                                                                                                   <  2:37474/148‑1 (MQ=255)
                             aaaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTc                                                                                                                  >  2:1043840/1‑148 (MQ=255)
                             aaaCTATGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCTTCACGCATATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTc                                                                                                                  >  2:1773670/1‑148 (MQ=255)
                              aaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTcc                                                                                                                 <  2:446553/148‑1 (MQ=255)
                              aaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTcc                                                                                                                 <  2:1861776/148‑1 (MQ=255)
                                 tttGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTa                                                                                                              <  1:3090730/148‑1 (MQ=255)
                                 tttGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTa                                                                                                              >  2:2984293/1‑148 (MQ=255)
                                 tttGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTa                                                                                                              <  2:1076207/148‑1 (MQ=255)
                                   tGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAAt                                                                                                            <  1:2076914/148‑1 (MQ=255)
                                      cTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGc                                                                                                         >  1:1887656/1‑148 (MQ=255)
                                       tttttCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCa                                                                                                        >  2:1118854/1‑148 (MQ=255)
                                        ttttCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCAc                                                                                                       <  1:2701904/148‑1 (MQ=255)
                                            ccACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACtata                                                                                                   >  1:3505415/1‑148 (MQ=255)
                                              acaTGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATaaa                                                                                                 <  1:672962/148‑1 (MQ=255)
                                               catgcatgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAAt                                                                                                <  2:1681653/148‑1 (MQ=255)
                                               catgcatgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAAt                                                                                                >  1:336756/1‑148 (MQ=255)
                                                 tgcatgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAAtct                                                                                              >  1:947933/1‑148 (MQ=255)
                                                  gcatgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAAtctc                                                                                             <  2:1249972/148‑1 (MQ=255)
                                                   catgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCa                                                                                            >  1:543607/1‑148 (MQ=255)
                                                          aaaCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGc                                                                                     >  2:617102/1‑148 (MQ=255)
                                                          aaaCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGc                                                                                     >  2:1308550/1‑148 (MQ=255)
                                                           aaCCATCCATATAATCATTTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGATTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCa                                                                                    <  2:1890562/148‑1 (MQ=255)
                                                            aCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCAc                                                                                   >  1:3274228/1‑148 (MQ=255)
                                                            aCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCAc                                                                                   >  1:3133098/1‑148 (MQ=255)
                                                             ccATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACAGCACt                                                                                  >  2:2431849/1‑148 (MQ=255)
                                                               aTCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTga                                                                                >  1:362216/1‑148 (MQ=255)
                                                                 ccAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTgaag                                                                              >  2:323038/1‑148 (MQ=255)
                                                                    gATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGaagaag                                                                           >  1:2392060/1‑148 (MQ=255)
                                                                     aTAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGt                                                                          <  1:843627/148‑1 (MQ=255)
                                                                        aTCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGCGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGt                                                                       >  2:2815226/1‑148 (MQ=255)
                                                                         tCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGtt                                                                      <  2:336756/148‑1 (MQ=255)
                                                                            ggTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTAt                                                                   <  1:1569859/148‑1 (MQ=255)
                                                                             gTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATc                                                                  >  1:1664896/1‑148 (MQ=255)
                                                                             gTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATc                                                                  <  1:1043840/148‑1 (MQ=255)
                                                                                tAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTAc                                                               >  2:3079300/1‑148 (MQ=255)
                                                                                   aTGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGt                                                            >  2:94454/1‑148 (MQ=255)
                                                                                     gTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCa                                                          >  1:108247/1‑148 (MQ=255)
                                                                                      tCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCaa                                                         <  2:1533971/148‑1 (MQ=255)
                                                                                          gTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAtttt                                                     <  1:2947236/148‑1 (MQ=255)
                                                                                            cACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAtttttt                                                   <  1:1974838/148‑1 (MQ=255)
                                                                                            cACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATATACCGTCAAtttttt                                                   >  1:1245586/1‑148 (MQ=255)
                                                                                              cGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTAc                                                 <  1:52011/148‑1 (MQ=255)
                                                                                               gattATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGAACTATAAATCTCATTACCGCACTGAAGATGTCGTTTATCTACCGTCAATTTTTTAcc                                                <  2:3167192/146‑1 (MQ=255)
                                                                                               gCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTAcc                                                <  1:733643/148‑1 (MQ=255)
                                                                                                 ttATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTAACCg                                              >  1:1645788/1‑148 (MQ=255)
                                                                                                   aTCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACACGCt                                            >  2:542119/1‑148 (MQ=255)
                                                                                                      tttAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATATACCGTCAATTTTTTAACCGCTGaa                                         >  1:918721/1‑148 (MQ=255)
                                                                                                       ttAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGaaa                                        >  2:2615695/1‑148 (MQ=255)
                                                                                                         aGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAAc                                      <  1:252958/148‑1 (MQ=255)
                                                                                                                    tCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGa                           <  1:1638082/148‑1 (MQ=255)
                                                                                                                       ggCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTg                        >  2:2944818/1‑148 (MQ=255)
                                                                                                                        gCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGa                       >  2:1210395/1‑148 (MQ=255)
                                                                                                                        gCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGa                       <  1:1059686/148‑1 (MQ=255)
                                                                                                                         cAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGaa                      <  2:2173044/148‑1 (MQ=255)
                                                                                                                           aTGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTAACCGCTGAAAACCAGGTAGCCGACTGAATg                    >  2:2366045/1‑148 (MQ=255)
                                                                                                                            tGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATgg                   >  2:2779570/1‑148 (MQ=255)
                                                                                                                                tATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCg               >  1:2469337/1‑148 (MQ=255)
                                                                                                                                 aTGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCgg              <  2:1524258/148‑1 (MQ=255)
                                                                                                                                  tGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGa             >  2:1659013/1‑148 (MQ=255)
                                                                                                                                   gggTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGAc            >  1:2903260/1‑148 (MQ=255)
                                                                                                                                    ggTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACg           >  2:903481/1‑148 (MQ=255)
                                                                                                                                      tGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGt         <  1:1163508/148‑1 (MQ=255)
                                                                                                                                       gAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTa        <  1:2765921/148‑1 (MQ=255)
                                                                                                                                         aaaTAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTAtt      >  2:735772/1‑148 (MQ=255)
                                                                                                                                          aaTAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTa     >  2:3126768/1‑148 (MQ=255)
                                                                                                                                             aaCTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTg  >  2:2858116/1‑148 (MQ=255)
                                                                                                                                             aaCTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTg  >  2:1105128/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTG  >  CP009273/2358497‑2358785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑