Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 201,962 A→T 100% D117V (GAT→GTT)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273201,9620AT98.7% 704.5 / ‑0.4 227D117V (GAT→GTT) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (108/115);  total (109/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

ATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG  >  CP009273/201818‑202106
                                                                                                                                                |                                                                                                                                                
aTTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttcg                                                                                                                                               >  2:3119980/1‑148 (MQ=255)
 ttttAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTcgc                                                                                                                                              >  2:692670/1‑148 (MQ=255)
    tAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGAc                                                                                                                                           <  2:1438660/148‑1 (MQ=255)
      aCGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTg                                                                                                                                         >  1:3235443/1‑148 (MQ=255)
         tCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCt                                                                                                                                      <  1:3009037/148‑1 (MQ=255)
              tGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCg                                                                                                                                 >  1:3438490/1‑148 (MQ=255)
                    ctgctgGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTaa                                                                                                                           <  1:1148108/148‑1 (MQ=255)
                         gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGaa                                                                                                                      <  2:1157789/148‑1 (MQ=255)
                         gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGaa                                                                                                                      >  1:3512485/1‑148 (MQ=255)
                           gTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAgg                                                                                                                    <  1:224315/148‑1 (MQ=255)
                            tgatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAggg                                                                                                                   >  1:3126748/1‑148 (MQ=255)
                             gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                  <  1:1518212/148‑1 (MQ=255)
                             gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                  >  2:238934/1‑148 (MQ=255)
                               tgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                <  1:2830374/148‑1 (MQ=255)
                                gaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGa                                                                                                               >  1:258240/1‑148 (MQ=255)
                                  gTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATc                                                                                                             <  2:3159218/148‑1 (MQ=255)
                                  gTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATc                                                                                                             <  2:3239623/148‑1 (MQ=255)
                                    tAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCct                                                                                                           <  2:1342205/148‑1 (MQ=255)
                                     aaCCCACCTGACGGTACTGGCGGCGAACATGACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTTTAAGCGCGGGTACGGTGCCGCCGGGCGGATCATCGTTCGCTACTGGCTTATAGATTTAAACGAAGGGTTGATCctt                                                                                                          <  1:3106867/148‑1 (MQ=255)
                                        ccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttctt                                                                                                       >  1:1034177/1‑148 (MQ=255)
                                        ccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttctt                                                                                                       >  1:1932223/1‑148 (MQ=255)
                                         cACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCttt                                                                                                      >  1:3248327/1‑148 (MQ=255)
                                             tGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcgg                                                                                                  <  1:1571391/148‑1 (MQ=255)
                                               aCGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcggcg                                                                                                >  1:976423/1‑148 (MQ=255)
                                                     cTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCa                                                                                          <  2:3207681/148‑1 (MQ=255)
                                                      tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACTGTGACGCCGGGCCGATCATCGTTGGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCAt                                                                                         <  1:3023097/148‑1 (MQ=255)
                                                      tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCAt                                                                                         >  2:3393598/1‑148 (MQ=255)
                                                        gcggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                       >  1:12024/1‑148 (MQ=255)
                                                        gcggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                       >  2:430051/1‑148 (MQ=255)
                                                         cggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATggg                                                                                      <  2:642/148‑1 (MQ=255)
                                                           gcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCg                                                                                    <  1:1445017/148‑1 (MQ=255)
                                                              aaCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACg                                                                                 >  2:2720704/1‑148 (MQ=255)
                                                               aCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGt                                                                                >  1:2874180/1‑148 (MQ=255)
                                                               aCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGt                                                                                >  1:824692/1‑148 (MQ=255)
                                                                  aTACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCgg                                                                             >  1:2901773/1‑148 (MQ=255)
                                                                    aCCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAc                                                                           >  2:2158200/1‑148 (MQ=255)
                                                                    aCCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAc                                                                           >  1:2098439/1‑148 (MQ=255)
                                                                     ccGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACg                                                                          >  1:3250074/1‑148 (MQ=255)
                                                                      cGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGc                                                                         <  2:2088454/148‑1 (MQ=255)
                                                                       ggCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCa                                                                        >  1:1248310/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     >  1:2250840/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     >  1:2250994/1‑148 (MQ=255)
                                                                          tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTc                                                                     <  2:2240763/148‑1 (MQ=255)
                                                                            tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                   >  2:3382216/1‑84 (MQ=255)
                                                                            tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                   <  1:649081/84‑1 (MQ=255)
                                                                               gAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                >  1:1774000/1‑148 (MQ=255)
                                                                               gAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                >  1:2630322/1‑148 (MQ=255)
                                                                                 aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  1:2753960/148‑1 (MQ=255)
                                                                                 aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  2:2952908/148‑1 (MQ=255)
                                                                                 aTCGGACGTTGCTGATCTCAACAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                              <  1:1641475/148‑1 (MQ=255)
                                                                                   cTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTg                                                            <  2:1862190/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2034902/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           >  2:3480652/1‑148 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2613503/148‑1 (MQ=255)
                                                                                    tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGGTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                           <  2:2509699/148‑1 (MQ=255)
                                                                                       cGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTaa                                                        <  1:1105014/148‑1 (MQ=255)
                                                                                        gTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAc                                                       >  1:2924773/1‑148 (MQ=255)
                                                                                            cTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcg                                                   <  2:2257666/148‑1 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 >  2:2931481/1‑148 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 <  1:3280222/148‑1 (MQ=255)
                                                                                              gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                 <  1:3280800/148‑1 (MQ=255)
                                                                                               aTCTCAAAAGCGTATCAGCGCGGGTCCGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcgc                                                >  2:186920/1‑148 (MQ=255)
                                                                                               aTCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcgc                                                >  1:3014128/1‑148 (MQ=255)
                                                                                                 ctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTg                                              >  2:2899865/1‑148 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTgg                                             <  1:2457095/148‑1 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTgg                                             <  2:1405469/148‑1 (MQ=255)
                                                                                                  tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCACGCTgg                                             >  2:1366881/1‑148 (MQ=255)
                                                                                                   cAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                            <  1:1271166/148‑1 (MQ=255)
                                                                                                   cAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                            <  2:2076206/148‑1 (MQ=255)
                                                                                                     aaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAg                                          >  1:994596/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         <  2:362125/148‑1 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         >  1:2021155/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         >  2:671338/1‑148 (MQ=255)
                                                                                                      aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                         <  1:448083/148‑1 (MQ=255)
                                                                                                       aGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGAt                                        <  2:3117594/148‑1 (MQ=255)
                                                                                                             tCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtgt                                  <  2:3015734/148‑1 (MQ=255)
                                                                                                                 cgcgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcg                              <  2:2171308/148‑1 (MQ=255)
                                                                                                                   cgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcgcg                            <  2:1222505/148‑1 (MQ=255)
                                                                                                                     ggTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtt                          <  1:3339525/148‑1 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         >  1:2359/1‑148 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         <  2:152471/148‑1 (MQ=255)
                                                                                                                      gTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                         <  1:2793343/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  1:1732838/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  2:2690752/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  1:591268/148‑1 (MQ=255)
                                                                                                                       tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                        <  2:740002/148‑1 (MQ=255)
                                                                                                                          ggTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATg                     <  2:834994/148‑1 (MQ=255)
                                                                                                                            tGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaa                   >  1:3197456/1‑148 (MQ=255)
                                                                                                                             gccgccGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaag                  <  1:2770454/148‑1 (MQ=255)
                                                                                                                              ccgccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTCACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaaga                 >  2:2196083/1‑148 (MQ=255)
                                                                                                                                   gggCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACt            <  2:1266298/148‑1 (MQ=255)
                                                                                                                                      ccGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTcc         <  1:1508198/148‑1 (MQ=255)
                                                                                                                                       cGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTccc        <  1:2720704/148‑1 (MQ=255)
                                                                                                                                         atcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCgg      >  2:2245462/1‑148 (MQ=255)
                                                                                                                                          tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGa     >  2:2803185/1‑148 (MQ=255)
                                                                                                                                          tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGa     >  1:1378070/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  1:1412192/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  <  1:430051/148‑1 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  2:1599270/1‑148 (MQ=255)
                                                                                                                                             tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg  >  1:821794/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCG  >  CP009273/201818‑202106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑