Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,370,987 C→A 100% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,370,9870CA100.0% 693.8 / NA 210E29* (GAA→TAA) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (110/100);  total (110/100)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

ATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC  >  CP009273/2370842‑2371130
                                                                                                                                                 |                                                                                                                                               
aTGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGt                                                                                                                                               >  2:3090858/1‑148 (MQ=255)
 tgttgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTg                                                                                                                                              >  1:179997/1‑148 (MQ=255)
    tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCa                                                                                                                                           <  1:1674380/148‑1 (MQ=255)
      taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                         <  1:771899/148‑1 (MQ=255)
      taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                         <  1:3090873/148‑1 (MQ=255)
      taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                         >  1:1218473/1‑148 (MQ=255)
      taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAAt                                                                                                                                         >  1:1625368/1‑148 (MQ=255)
        actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCg                                                                                                                                       >  2:1736142/1‑148 (MQ=255)
        actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCg                                                                                                                                       >  2:2696379/1‑148 (MQ=255)
        actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCg                                                                                                                                       >  2:2695923/1‑148 (MQ=255)
                aGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                               <  1:1833094/148‑1 (MQ=255)
                aGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaa                                                                                                                               >  2:1190099/1‑148 (MQ=255)
                 ggTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGaaaaa                                                                                                                              >  2:757486/1‑148 (MQ=255)
                    tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                           <  1:1900882/148‑1 (MQ=255)
                    tttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCg                                                                                                                           >  2:2062406/1‑148 (MQ=255)
                      tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTg                                                                                                                         <  2:1032413/148‑1 (MQ=255)
                            gTAAGTCGGTGACATCATCGAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                   <  2:1073015/148‑1 (MQ=255)
                            gTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                   <  2:1122460/148‑1 (MQ=255)
                            gTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCaaaaa                                                                                                                   <  2:3308113/148‑1 (MQ=255)
                             tAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAac                                                                                                                  <  1:3478378/148‑1 (MQ=255)
                             tAAGTCGGTGAAATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAac                                                                                                                  <  2:1833521/148‑1 (MQ=255)
                              aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAaca                                                                                                                 >  2:1171449/1‑148 (MQ=255)
                                gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                               <  2:2402187/148‑1 (MQ=255)
                                gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                               <  2:609015/148‑1 (MQ=255)
                                gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                               >  2:1968547/1‑148 (MQ=255)
                                gTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                                                                               >  2:1511436/1‑148 (MQ=255)
                                   ggTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                            >  2:302692/1‑148 (MQ=255)
                                     tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                          <  1:2278878/148‑1 (MQ=255)
                                     tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                          <  2:173194/148‑1 (MQ=255)
                                     tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGCAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                                                                          <  1:2072231/148‑1 (MQ=255)
                                      gACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagccag                                                                                                         <  2:2608916/148‑1 (MQ=255)
                                           catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                    >  1:1684559/1‑148 (MQ=255)
                                           catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                    >  2:257516/1‑148 (MQ=255)
                                           catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                    <  2:1040192/148‑1 (MQ=255)
                                           catcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaa                                                                                                    >  1:116118/1‑148 (MQ=255)
                                            atcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaaa                                                                                                   <  2:1482281/148‑1 (MQ=255)
                                            atcaAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaaa                                                                                                   >  2:400894/1‑148 (MQ=255)
                                              caAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGATTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAcc                                                                                                 <  1:2964827/148‑1 (MQ=255)
                                              caAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAcc                                                                                                 <  2:468845/148‑1 (MQ=255)
                                              caAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAcc                                                                                                 <  2:2373189/148‑1 (MQ=255)
                                               aaaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCt                                                                                                <  2:1014163/148‑1 (MQ=255)
                                               aaaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCt                                                                                                <  1:3450324/148‑1 (MQ=255)
                                                aatcaatcGAGGCTAATCGCCGCCGAACCACCGTTACCTGGGATATCAACATACAACCGTGTTTAGTCGGAAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTg                                                                                               <  1:602784/148‑1 (MQ=255)
                                                aaTCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTg                                                                                               >  1:1091343/1‑148 (MQ=255)
                                                  tCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGt                                                                                             >  1:2458033/1‑148 (MQ=255)
                                                   ccATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtt                                                                                            <  2:1868062/148‑1 (MQ=255)
                                                   ccATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGtt                                                                                            >  1:2916351/1‑148 (MQ=255)
                                                       cGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                                                                        >  1:2761557/1‑148 (MQ=255)
                                                         aCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgt                                                                                      <  1:3319159/148‑1 (MQ=255)
                                                          cGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGTGTAAACCTGGTTTTCCgtg                                                                                     >  1:2248022/1‑148 (MQ=255)
                                                          cGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtg                                                                                     <  1:1391030/148‑1 (MQ=255)
                                                           gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                                                                    <  1:1171519/148‑1 (MQ=255)
                                                           gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                                                                    >  1:1445602/1‑148 (MQ=255)
                                                            cTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtt                                                                                   >  1:301760/1‑148 (MQ=255)
                                                                tCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGACATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGc                                                                               >  2:3156196/1‑148 (MQ=255)
                                                                 cgccgcCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                              >  1:2270351/1‑148 (MQ=255)
                                                                 cgccgcCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                              <  2:1461851/148‑1 (MQ=255)
                                                                        gAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCACTCGCCGGAAAAACCGTGCAAAAACACCAGCCCGGGTAAACCGGGTTTTCCGTGTTTTGCCTGCGCGt                                                                       <  2:486284/148‑1 (MQ=255)
                                                                         aaCCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTg                                                                      <  1:1965339/148‑1 (MQ=255)
                                                                          accaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGc                                                                     <  1:3059628/148‑1 (MQ=255)
                                                                             accGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAgg                                                                  <  1:2456037/148‑1 (MQ=255)
                                                                               cGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAt                                                                >  1:3474905/1‑148 (MQ=255)
                                                                                 tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCa                                                              <  2:1575689/148‑1 (MQ=255)
                                                                                   aCCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                            >  2:2067798/1‑148 (MQ=255)
                                                                                     cTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaaa                                                          <  1:3058680/148‑1 (MQ=255)
                                                                                       gggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATg                                                        <  1:684996/148‑1 (MQ=255)
                                                                                         gagaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc                                                      <  2:179997/148‑1 (MQ=255)
                                                                                            aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                   >  2:1086017/1‑148 (MQ=255)
                                                                                            aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                   <  1:1739015/148‑1 (MQ=255)
                                                                                            aTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAAAACCAGCCAGGGTAAACCTGGTTTTCCGGGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTa                                                   >  1:2362035/1‑148 (MQ=255)
                                                                                             tCAACATACCACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCGTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAc                                                  <  2:3287652/148‑1 (MQ=255)
                                                                                             tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAc                                                  <  2:1545380/148‑1 (MQ=255)
                                                                                             tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCGTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAc                                                  <  1:2256730/148‑1 (MQ=255)
                                                                                              cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                 <  2:1312262/148‑1 (MQ=255)
                                                                                              cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                 >  1:904326/1‑148 (MQ=255)
                                                                                              cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                 >  1:2658687/1‑148 (MQ=255)
                                                                                              cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTAcc                                                 >  2:1327697/1‑148 (MQ=255)
                                                                                                aCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTg                                               <  2:137593/148‑1 (MQ=255)
                                                                                                 cATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgc                                              >  2:2041851/1‑148 (MQ=255)
                                                                                                   tACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTgcgc                                            <  1:1583998/148‑1 (MQ=255)
                                                                                                     cAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCa                                          >  1:1079285/1‑148 (MQ=255)
                                                                                                         cGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAg                                      <  2:1647324/148‑1 (MQ=255)
                                                                                                           tGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGtt                                    >  1:1230913/1‑148 (MQ=255)
                                                                                                             agtagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGc                                  <  1:2062406/148‑1 (MQ=255)
                                                                                                             agtagtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGc                                  <  1:455030/148‑1 (MQ=255)
                                                                                                                agtCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTgg                               <  2:2878563/148‑1 (MQ=255)
                                                                                                                  tCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                             >  2:554706/1‑148 (MQ=255)
                                                                                                                  tCGGCAAACGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                             >  1:2179223/1‑148 (MQ=255)
                                                                                                                         aCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgc                      >  2:1154296/1‑148 (MQ=255)
                                                                                                                         aCGCCTCGCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgc                      >  1:988717/1‑148 (MQ=255)
                                                                                                                                gCCCACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCAt               >  1:3145356/1‑148 (MQ=255)
                                                                                                                                  ccACTTCTTGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCCTCg             >  2:3446179/1‑148 (MQ=255)
                                                                                                                                       tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTc        >  2:1650010/1‑148 (MQ=255)
                                                                                                                                       tcttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTc        <  1:3177224/148‑1 (MQ=255)
                                                                                                                                         ttGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGt      <  1:1287540/148‑1 (MQ=255)
                                                                                                                                          tGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt     >  1:1651153/1‑148 (MQ=255)
                                                                                                                                          tGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt     >  2:181453/1‑148 (MQ=255)
                                                                                                                                          tGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt     >  2:2259847/1‑148 (MQ=255)
                                                                                                                                          tGCCATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt     <  1:518176/148‑1 (MQ=255)
                                                                                                                                             cATTAGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAac  >  1:119130/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAAC  >  CP009273/2370842‑2371130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑