BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,400,858	C→A	100%	intergenic (‑738/‑182)	lrhA ← / → alaA	transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,400,858	0	C	A	97.8%	714.7 / 5.6	228	intergenic (‑738/‑182)	lrhA/alaA	transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
Reads supporting (aligned to +/- strand): ref base C (0/5); new base A (93/129); total (93/135)
Fisher's exact test for biased strand distribution p-value = 8.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 228 total aligned reads displayed.

CGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACG  >  CP009273/2400713‑2401001

    gTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaa                                                                                                                                           <  1:512366/148‑1 (MQ=255)

     ttAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaaa                                                                                                                                          >  2:2798687/1‑148 (MQ=255)

     ttAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaaa                                                                                                                                          >  1:16243/1‑148 (MQ=255)

      tAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAg                                                                                                                                         >  2:2087405/1‑148 (MQ=255)

        aaCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCa                                                                                                                                       <  1:2513796/148‑1 (MQ=255)

        aaCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCa                                                                                                                                       <  1:1574911/148‑1 (MQ=255)

        aaCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCa                                                                                                                                       <  2:2895554/148‑1 (MQ=255)

            ctcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCAtata                                                                                                                                   <  2:2979705/148‑1 (MQ=255)

              ccGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATaca                                                                                                                                 <  1:2733306/148‑1 (MQ=255)

                gTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATCCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACAcc                                                                                                                               >  1:448990/1‑148 (MQ=255)

                   aTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCa                                                                                                                            <  2:511192/148‑1 (MQ=255)

                   aTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCa                                                                                                                            <  1:1585/148‑1 (MQ=255)

                   aTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCa                                                                                                                            <  1:297894/148‑1 (MQ=255)

                    tGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                           >  2:617877/1‑148 (MQ=255)

                    tGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAAGTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                           <  2:1818598/148‑1 (MQ=255)

                     gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCatt                                                                                                                          <  1:3048983/148‑1 (MQ=255)

                        gAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCattatt                                                                                                                       <  1:2625355/148‑1 (MQ=255)

                         aaaTATCATCATCGCTTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                      <  1:1797323/148‑1 (MQ=255)

                         aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                      >  1:1019756/1‑148 (MQ=255)

                            tatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGt                                                                                                                   <  2:1930216/148‑1 (MQ=255)

                             atcataataGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTTAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTc                                                                                                                  <  1:2060207/148‑1 (MQ=255)

                                atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCatt                                                                                                               >  2:3220688/1‑148 (MQ=255)

                                  catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                             <  1:2217130/148‑1 (MQ=255)

                                  catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                             <  1:1959727/148‑1 (MQ=255)

                                  catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                             >  2:1034513/1‑148 (MQ=255)

                                   atcTCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattatt                                                                                                            <  2:542548/148‑1 (MQ=255)

                                    tcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCAttatta                                                                                                           >  2:237330/1‑148 (MQ=255)

                                    tcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCAttatta                                                                                                           <  2:213671/148‑1 (MQ=255)

                                        gTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtt                                                                                                       <  2:28612/148‑1 (MQ=255)

                                         tttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                      >  1:246134/1‑148 (MQ=255)

                                         tttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                      >  1:2760747/1‑148 (MQ=255)

                                         tttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                      >  2:2742056/1‑148 (MQ=255)

                                          ttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttat                                                                                                     >  1:746426/1‑148 (MQ=255)

                                            cATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttatta                                                                                                   >  2:143532/1‑148 (MQ=255)

                                              ttAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAc                                                                                                 <  1:2008463/148‑1 (MQ=255)

                                              ttAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAc                                                                                                 <  2:881544/148‑1 (MQ=255)

                                                    aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                           <  2:958944/148‑1 (MQ=255)

                                                    aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                           <  1:2463482/148‑1 (MQ=255)

                                                     aTTCTTAAATGAGTCATTTTCACACAATTATCTTACAGATTAAAAATCCTTACTTACAATTATGAATCTGAACAATTCACTATATTACTTTGTATGTAAAGCATATTCTCCTGATTATTTTGTCATTATTTAGTTTTTAACatgagat                                                                                          <  2:225744/148‑7 (MQ=255)

                                                     aTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACaa                                                                                          <  1:2094129/148‑1 (MQ=255)

                                                          tAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAg                                                                                     >  1:2195751/1‑148 (MQ=255)

                                                            aaTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGcc                                                                                   <  2:1990601/148‑1 (MQ=255)

                                                             aTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                  >  2:1362255/1‑148 (MQ=255)

                                                             aTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                  >  2:3108987/1‑148 (MQ=255)

                                                             aTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                  >  1:1495278/1‑148 (MQ=255)

                                                              tGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCtt                                                                                 <  1:2812179/148‑1 (MQ=255)

                                                               gAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTc                                                                                >  2:1777688/1‑148 (MQ=255)

                                                                aGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTcc                                                                               <  2:396214/148‑1 (MQ=255)

                                                                 ggCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTccc                                                                              <  1:3327496/148‑1 (MQ=255)

                                                                   cATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCct                                                                            <  1:652129/148‑1 (MQ=255)

                                                                      tttCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTg                                                                         <  1:1386479/148‑1 (MQ=255)

                                                                         cacacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTcc                                                                               <  1:241760/139‑1 (MQ=255)

                                                                           cacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaa                                                                    >  1:326379/1‑148 (MQ=255)

                                                                                 tATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCACTGGCAAAATCTTa                                                              >  1:3199509/1‑148 (MQ=255)

                                                                                 tATCTTACAGATAAAAAAACCAGACTCACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTa                                                              >  2:1734311/1‑148 (MQ=255)

                                                                                    cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTc                                                           <  1:813880/148‑1 (MQ=255)

                                                                                    cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTc                                                           <  1:1071153/148‑1 (MQ=255)

                                                                                     ttACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCt                                                          >  2:2927373/1‑148 (MQ=255)

                                                                                           aTAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAc                                                    >  2:1160562/1‑148 (MQ=255)

                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   <  2:3330359/148‑1 (MQ=255)

                                                                                             aaaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                  >  2:645099/1‑148 (MQ=255)

                                                                                             aaaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                  <  1:2427335/148‑1 (MQ=255)

                                                                                             aaaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                  >  2:2258752/1‑148 (MQ=255)

                                                                                              aaaaaaCCAGACTTACAATTAATAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAACCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                 <  2:1977388/148‑1 (MQ=255)

                                                                                              aaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                 <  1:1233628/148‑1 (MQ=255)

                                                                                              aaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                 <  1:912178/148‑1 (MQ=255)

                                                                                                 aaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaa                                              >  1:2318744/1‑148 (MQ=255)

                                                                                                   aCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaaaa                                            >  1:349718/1‑148 (MQ=255)

                                                                                                   aCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCaaaa                                            <  2:1545162/148‑1 (MQ=255)

                                                                                                     cAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAaca                                          >  1:2707538/1‑148 (MQ=255)

                                                                                                      aGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAacac                                         <  2:706830/148‑1 (MQ=255)

                                                                                                      aGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAacac                                         <  2:256230/148‑1 (MQ=255)

                                                                                                       gACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACAcc                                        <  1:998212/148‑1 (MQ=255)

                                                                                                       gACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTAAAAACAcc                                        >  2:118067/1‑148 (MQ=255)

                                                                                                        aCTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCACAACACCg                                       >  2:3408853/1‑148 (MQ=255)

                                                                                                        aCTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCg                                       >  1:3165490/1‑148 (MQ=255)

                                                                                                         cTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGt                                      >  2:2602547/1‑148 (MQ=255)

                                                                                                          ttACAATTAAGAATCAGAACAATTCACTATATAACATTTAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                     <  2:2888308/148‑1 (MQ=255)

                                                                                                          ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                     <  1:2828027/148‑1 (MQ=255)

                                                                                                          ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                     >  1:1517377/1‑148 (MQ=255)

                                                                                                          ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                     >  2:2589388/1‑148 (MQ=255)

                                                                                                             cAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTg                                  <  2:2790556/148‑1 (MQ=255)

                                                                                                             cAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTg                                  >  2:747312/1‑148 (MQ=255)

                                                                                                                ttAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTgggg                               <  1:2569085/148‑1 (MQ=255)

                                                                                                                 tAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTggggg                              <  1:1165696/148‑1 (MQ=255)

                                                                                                                       tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                        <  2:1610037/148‑1 (MQ=255)

                                                                                                                       tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                        <  2:2760747/148‑1 (MQ=255)

                                                                                                                        cAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACAt                       <  2:479502/148‑1 (MQ=255)

                                                                                                                            aCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGtt                   <  1:1502899/148‑1 (MQ=255)

                                                                                                                            aCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGtt                   <  1:2234025/148‑1 (MQ=255)

                                                                                                                            aCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGtt                   <  1:2233876/148‑1 (MQ=255)

                                                                                                                                 tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAg              >  1:827599/1‑148 (MQ=255)

                                                                                                                                  cACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGc             <  1:865293/148‑1 (MQ=255)

                                                                                                                                   aCTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt            <  1:101358/148‑1 (MQ=255)

                                                                                                                                     tatataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGa          >  2:3319385/1‑148 (MQ=255)

                                                                                                                                     tatataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGa          <  1:3138365/148‑1 (MQ=255)

                                                                                                                                        ataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACtt       >  2:695591/1‑148 (MQ=255)

                                                                                                                                         taACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTc      <  1:617877/148‑1 (MQ=255)

                                                                                                                                          aaCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTcc     <  1:2258752/148‑1 (MQ=255)

                                                                                                                                          aaCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTcc     >  2:1906135/1‑148 (MQ=255)

CGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACG  >  CP009273/2400713‑2401001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`