Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA98.6% 669.0 / 0.5 208A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (120/85);  total (122/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404629‑2404916
                                                                                                                                               |                                                                                                                                                
tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaa                                                                                                                                              >  2:2075155/1‑148 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                       >  2:1955506/1‑148 (MQ=255)
         ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                     >  1:1239426/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    >  1:2056343/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    >  2:815587/1‑148 (MQ=255)
          aaaactcggcgccAATCAGGAAAACTTTCGTATAAGAAACGCCTTAAAATACTTCTGGGTCTGTCCGCGTTCCGATTAATCAAAACGCTACAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                    <  2:1585267/141‑1 (MQ=255)
             aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGc                                                                                                                                 >  1:2618076/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCac                                                                                                                               >  2:3485827/1‑147 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCaa                                                                                                                               >  1:2604278/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGAAGCAAACAAGACGATGGCaa                                                                                                                               >  2:2839499/1‑148 (MQ=255)
                aGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                              >  2:2528352/1‑148 (MQ=255)
                aGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAAc                                                                                                                              >  1:1514114/1‑148 (MQ=255)
                 ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                             >  1:1289061/1‑148 (MQ=255)
                     gcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCa                                                                                                                         >  2:341549/1‑148 (MQ=255)
                           aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                                                   >  1:3493390/1‑148 (MQ=255)
                           aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                                                   >  1:3493379/1‑148 (MQ=255)
                             gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                 >  1:153315/1‑148 (MQ=255)
                             gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                                                 >  1:1928024/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAACAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:2539774/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:3314292/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                               >  2:3234500/1‑148 (MQ=255)
                                   tttAGTATCACAAAAGCCGTAAAATACTTCTGGGACTGTCCGCGTTCCGATAAATCCAAACGCGACAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                                                                                                           <  2:3151876/147‑1 (MQ=255)
                                     tAGTATCATAAACGCCTTAAAATAGTTGGGGTACTGTCAGCGTTCCGCTTAATCAAAACGCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                                                         <  2:160959/148‑1 (MQ=255)
                                          tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                    >  2:3008327/1‑148 (MQ=255)
                                             gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAACGAACCCAAATCAAtt                                                                                                 >  2:2008019/1‑148 (MQ=255)
                                              aaaaGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGAAAACGCTAAATTAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                <  2:1755747/148‑1 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:2622113/1‑148 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:600762/1‑148 (MQ=255)
                                              aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                                                >  2:2974908/1‑148 (MQ=255)
                                               aacgCCGTAAATTAGTTCTGGTACTTTCAGGTTTCCGATTAATCAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                               <  2:723543/145‑1 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCATCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATACAAACGCACTCTGCCCGTAGCAAACAAGACGATGGAAACGGCCAGTAATGAAAGAACCCAAATCAAATCAc                                                                                              >  1:507521/1‑148 (MQ=255)
                                                 aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                             >  1:2536217/1‑148 (MQ=255)
                                                 aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                             >  2:1244749/1‑148 (MQ=255)
                                                  gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                            >  2:698221/1‑148 (MQ=255)
                                                  gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                            >  1:414913/1‑148 (MQ=255)
                                                     ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                         >  2:1939215/1‑148 (MQ=255)
                                                       aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                                                       >  2:2271/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                     >  1:1774775/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTAAGGGTTCCGCTTAATGAAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                     <  2:2618076/148‑1 (MQ=255)
                                                          aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAAc                                                                                    >  2:1003412/1‑148 (MQ=255)
                                                                 tGGGAGTGTCCGGTTTCCGTTTAATGCAACCGCCAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCt                                                                             <  1:2909904/148‑1 (MQ=255)
                                                                          cAGGGTTCCGATTAATCAAAACGCCACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    <  2:2339669/148‑1 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    >  2:1015530/1‑148 (MQ=255)
                                                                          aaGCGTTCCGCTTATTGAAAACCCTAAAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAACGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                    <  2:2640528/147‑1 (MQ=255)
                                                                               ttCCGCTTAATCAAACCGCCAAAAAAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg                                                               <  2:669540/148‑1 (MQ=255)
                                                                                tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              >  2:1725738/1‑148 (MQ=255)
                                                                                tCCCCTTAATCAAAACCCGAAAAAAACAAACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                              <  2:2470853/148‑1 (MQ=255)
                                                                                 ccGATTAATCAAAACGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                                             <  2:1466650/148‑1 (MQ=255)
                                                                                    cTTACTCCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                          <  2:692127/148‑1 (MQ=255)
                                                                                     ttAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                                                         >  2:1575757/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:824359/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:834411/1‑148 (MQ=255)
                                                                                      tAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                                                        >  1:924193/1‑148 (MQ=255)
                                                                                         tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                     >  1:683953/1‑148 (MQ=255)
                                                                                         tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                     >  2:1395293/1‑148 (MQ=255)
                                                                                          gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                    >  2:2455294/1‑148 (MQ=255)
                                                                                           cAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                   >  1:2693848/1‑148 (MQ=255)
                                                                                                  taaaaTAACAAACACAGCGACCGCATCCATACGCACTCGGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCAt                                            <  2:3013649/145‑1 (MQ=255)
                                                                                                      aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                        <  2:1289236/148‑1 (MQ=255)
                                                                                                       tAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       >  2:2170238/1‑148 (MQ=255)
                                                                                                       tAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       >  2:2040360/1‑148 (MQ=255)
                                                                                                       aaaaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                       <  1:2344211/144‑1 (MQ=255)
                                                                                                         acaaacaaAGCGACCGCATCCATACGAAATCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAAACAAATAAATTCACCTTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                     >  1:2278705/1‑148 (MQ=255)
                                                                                                         aaaaacaccGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                     <  1:295924/146‑1 (MQ=255)
                                                                                                           aaacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                   <  1:2990855/148‑1 (MQ=255)
                                                                                                            aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                  >  2:1371084/1‑148 (MQ=255)
                                                                                                            aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                  >  1:729480/1‑148 (MQ=255)
                                                                                                               aacgCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcga                               <  2:1334707/145‑1 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             <  2:405773/148‑1 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  1:1372249/1‑148 (MQ=255)
                                                                                                                 aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATACACCGTTCACAACTTATCATTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                             >  1:593627/1‑148 (MQ=255)
                                                                                                                   cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                           >  1:2802713/1‑148 (MQ=255)
                                                                                                                    gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                          <  1:977572/148‑1 (MQ=255)
                                                                                                                     aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCTACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                         <  1:1384479/148‑1 (MQ=255)
                                                                                                                     aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                         >  1:107526/1‑148 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCATTGAGGGGGATGACTTGATTCTGCCATACAAAAGCCCCGACGAGACgg                       >  1:3238305/1‑148 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                       <  1:1003389/148‑1 (MQ=255)
                                                                                                                       cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                       <  1:1004222/148‑1 (MQ=255)
                                                                                                                        gCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                      <  1:2176758/148‑1 (MQ=255)
                                                                                                                         cATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                     >  1:1408014/1‑148 (MQ=255)
                                                                                                                          aTCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGc                    >  1:2372292/1‑148 (MQ=255)
                                                                                                                            ccATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                  >  2:2676797/1‑148 (MQ=255)
                                                                                                                              agaCGAACTATGCCCGTATCAAACAAGACTATGGCAACGGCCATTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGTGATGACTTTATTCTGCCATAAAAAAGCCCCGACGAGACGTGGCTaaa                <  2:1285468/146‑1 (MQ=255)
                                                                                                                              aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                <  1:3091913/148‑1 (MQ=255)
                                                                                                                              aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                <  1:533208/148‑1 (MQ=255)
                                                                                                                                aCGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc              >  2:91691/1‑148 (MQ=255)
                                                                                                                                 cGCACTCTGCCCGTATCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa             <  2:1900952/148‑1 (MQ=255)
                                                                                                                                   cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           <  2:3493385/148‑1 (MQ=255)
                                                                                                                                   cACTCTGCCCGTAGAAAACAAGACGAGGGCAACGGCAAGTAATTAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg           <  1:3271029/148‑1 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          <  2:1239426/148‑1 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          >  2:2857586/1‑148 (MQ=255)
                                                                                                                                    aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa          <  1:1682405/148‑1 (MQ=255)
                                                                                                                                     ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt         <  1:2848974/148‑1 (MQ=255)
                                                                                                                                     ctctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATAAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt         <  2:2218447/148‑1 (MQ=255)
                                                                                                                                      tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc        >  1:2148556/1‑148 (MQ=255)
                                                                                                                                       ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa       <  2:460285/148‑1 (MQ=255)
                                                                                                                                       ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa       <  1:207649/148‑1 (MQ=255)
                                                                                                                                        tGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAg      <  2:1483859/148‑1 (MQ=255)
                                                                                                                                            cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  2:1514116/148‑1 (MQ=255)
                                                                                                                                            cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtgt  <  1:2253620/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGT  >  CP009273/2404629‑2404916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑