| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,467,970 | G→T | 100% | D154Y (GAC→TAC) | yfdQ → | CPS‑53 (KpLE1) prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,467,970 | 0 | G | T | 97.3% | 587.8 / 6.2 | 202 | D154Y (GAC→TAC) | yfdQ | CPS‑53 (KpLE1) prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (91/103); total (94/108) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 202 total aligned reads displayed.
AACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGACATTATGC > CP009273/2467829‑2468114 | acacCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCAt > 1:1774639/1‑148 (MQ=255) acCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTc < 1:283476/148‑1 (MQ=255) acCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTc > 2:1033807/1‑148 (MQ=255) cGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAg > 1:1670029/1‑148 (MQ=255) gCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAgg < 1:1333541/148‑1 (MQ=255) cACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGca > 1:1886318/1‑148 (MQ=255) aCTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaa > 1:1968987/1‑148 (MQ=255) aCTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaa > 2:2294286/1‑148 (MQ=255) aCTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaa < 2:3393551/148‑1 (MQ=255) ctctCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaac > 1:908229/1‑148 (MQ=255) ctctCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaac > 1:1277413/1‑148 (MQ=255) ctcAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGcaacaa < 1:1089085/148‑1 (MQ=255) cAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACaaaa < 2:3053507/148‑1 (MQ=255) cAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACaaaa > 1:337064/1‑148 (MQ=255) aaaCTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAg < 1:806177/148‑1 (MQ=255) aCTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGTTTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCg < 1:1576151/148‑1 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCgg > 1:1008257/1‑148 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCgg < 1:2416965/148‑1 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCgg > 2:1439030/1‑148 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCgg < 2:38659/148‑1 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCgg > 2:3184730/1‑148 (MQ=255) cTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCgg < 1:3037476/148‑1 (MQ=255) tGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGc > 1:1731831/1‑148 (MQ=255) aaaaaGACCGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCACAAATCACTGGCAGAATGGATTGAAGACTGGGCCGAATACCTTGTGGGCTTTGATGCTAATGGTTACGCCTTTCAGGCAACAAAAGCGGCTg < 2:1290563/148‑1 (MQ=255) aaaGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAGGCGGCTGCg > 2:1767762/1‑148 (MQ=255) aaaGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCg > 1:2814463/1‑148 (MQ=255) aaaGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCg > 1:3315215/1‑148 (MQ=255) gACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCa > 1:50208/1‑148 (MQ=255) cAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAAt < 2:225799/148‑1 (MQ=255) aGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGACTGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATc > 2:239161/1‑148 (MQ=255) gTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTaaa < 2:2155981/148‑1 (MQ=255) ttCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTaaaa > 2:569179/1‑148 (MQ=255) tctcTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAAt > 1:2861125/1‑148 (MQ=255) gCCCTGTTGTCTGTTAAGGGCTAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAAAAAAAGCGGCTGCGGCAATCCGTAAAATCACGa < 2:1918396/148‑1 (MQ=255) gCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACGGGCAGATTGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTATTGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGa < 1:159766/148‑1 (MQ=255) gttgtCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGaa > 1:135943/1‑148 (MQ=255) ttgtCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAg < 2:2012829/148‑1 (MQ=255) tCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTTATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCaa < 2:8807/148‑1 (MQ=255) cTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGTTTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCaaa < 2:1057725/148‑1 (MQ=255) cTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCaaa > 1:503305/1‑148 (MQ=255) ttAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACGTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCa < 2:2750376/148‑1 (MQ=255) ttAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCa > 2:1664381/1‑148 (MQ=255) aaCGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGa < 1:2908533/148‑1 (MQ=255) aaCGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGa < 2:1185974/148‑1 (MQ=255) aaCGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGa < 2:2261053/148‑1 (MQ=255) aCGGCGAGCGTACCTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGAc < 1:143408/148‑1 (MQ=255) aCGGCGAGCGTAACTCCCACAAATCCCTGGCAGATTGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTTATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGAc < 1:143456/148‑1 (MQ=255) gcgcgcGTAACGCCCAGAAACCAGGGGCAGAATGGATTGAAGCCGGGGCCGACTACCTTGTGGGCTTTGATGCTATTGGTTACGCCATTCAGCCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGc < 1:1807807/148‑1 (MQ=255) aGCGTAACTCCCATAAATCACTGGCAGAATTGATTTAAGACTGGGCCGACTACTTTGTTGGCTTTGATGCTATTGTTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGa < 2:2990970/148‑1 (MQ=255) gCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGAt > 1:1285866/1‑148 (MQ=255) cGTAACTCCCACAAATCACGGGCAGATTGGATTCACGACTGGCCCCAATACCTTGGGGGCTTTGAGGATAATGGTAACCCAATACAGCAAAAAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGAtt < 1:1492682/148‑1 (MQ=255) tAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGAtttt < 2:2234342/148‑1 (MQ=255) tAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGAtttt < 2:2207426/148‑1 (MQ=255) cccAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGa < 1:1452252/148‑1 (MQ=255) cAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGGGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATa < 2:2069695/148‑1 (MQ=255) aGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATaa > 2:3238826/1‑148 (MQ=255) gAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAAt > 2:301022/1‑148 (MQ=255) gAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAAt > 2:302196/1‑148 (MQ=255) gAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAAt > 2:3434138/1‑148 (MQ=255) gAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAAt > 2:682628/1‑148 (MQ=255) aaaTCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATg > 1:637746/1‑148 (MQ=255) aaaTCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCGTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATg < 2:805292/148‑1 (MQ=255) aaaTCACGGGCATAATGGATTGAAGACGGGGCCGACTACCTTGTGGGCTTTGATGATAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATg < 1:3138429/148‑1 (MQ=255) aaTCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGa < 2:130934/148‑1 (MQ=255) tCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACt > 2:2905043/1‑148 (MQ=255) cACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACtt > 1:1408692/1‑148 (MQ=255) aCTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTc > 1:384928/1‑148 (MQ=255) tGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAg > 1:3410895/1‑148 (MQ=255) ggCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGc > 1:213561/1‑148 (MQ=255) ggCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGc > 1:213840/1‑148 (MQ=255) ggCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGc < 2:3141621/148‑1 (MQ=255) gCAGAATGTATTGAAGACGGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGAAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCg < 1:2126006/148‑1 (MQ=255) gCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCg > 2:1516034/1‑148 (MQ=255) aaTGTATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCAATTCAGCCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCaa < 1:347047/148‑1 (MQ=255) aTGGATTGAAGTCGGGCCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCaaa < 2:1979742/148‑1 (MQ=255) tGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTACTGGTTACGCCATTCAGGCAAAAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTcc < 2:602207/148‑1 (MQ=255) tGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTcc < 2:2485375/148‑1 (MQ=255) aCTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGAt < 1:3115386/148‑1 (MQ=255) gggCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAAAAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGa < 1:1128590/148‑1 (MQ=255) ggCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGaa < 1:1769697/148‑1 (MQ=255) gCCGACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAAAAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAAt < 1:1040856/148‑1 (MQ=255) gACTACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTg > 2:2657693/1‑148 (MQ=255) aCCTTGTTGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATTACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga < 2:2773933/148‑1 (MQ=255) aCCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga > 1:2960479/1‑148 (MQ=255) aCCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga > 1:3515374/1‑148 (MQ=255) aCCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga < 2:2620506/148‑1 (MQ=255) aCCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga < 2:861540/148‑1 (MQ=255) aCCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcga < 2:3484947/148‑1 (MQ=255) ccTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcgaa < 2:607030/148‑1 (MQ=255) cTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcgaag > 1:2048923/1‑148 (MQ=255) ttGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGACTCTGTcgaagc > 2:3503094/1‑148 (MQ=255) tgGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcgaagcgaa > 2:1332906/1‑148 (MQ=255) gggCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTcgaagcgaag > 1:2627838/1‑148 (MQ=255) ggCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGa < 1:2294237/148‑1 (MQ=255) cTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGAcc < 2:127170/148‑1 (MQ=255) ttGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCTTAAAATCACGATTGAAGCAAACCAGACCGATGATTTTGAAGATAATGACTTAAGAGGAAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCaa > 1:2744478/1‑148 (MQ=255) aTGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGa > 2:192849/1‑148 (MQ=255) gCTATTGGTTACGACATTCAGCAAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGACa < 1:1521512/148‑1 (MQ=255) gCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGACa < 1:225806/148‑1 (MQ=255) ggTTACGCCTTTCAGGCAACAAACGCGGCTGCGGCAATCCGTAAACTCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGACATTATGc < 1:3432231/148‑1 (MQ=255) | AACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGAAGATAATGACTTCAGCGGCAAACGCTCCCTGATGGAATCTGTCGAAGCGAAGACCAAAGACATTATGC > CP009273/2467829‑2468114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |