Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 214,084 G→T 100% G393G (GGC→GGA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273214,0840GT99.5% 611.6 / ‑4.3 186G393G (GGC→GGA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (84/101);  total (84/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGA  >  CP009273/213939‑214228
                                                                                                                                                 |                                                                                                                                                
ccATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtcc                                                                                                                                                <  2:94354/148‑1 (MQ=255)
 cATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtcca                                                                                                                                               <  1:3299308/148‑1 (MQ=255)
  aTCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccag                                                                                                                                              >  2:2357095/1‑148 (MQ=255)
   tCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagc                                                                                                                                             <  2:2855062/148‑1 (MQ=255)
    cGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagcc                                                                                                                                            >  1:2058659/1‑148 (MQ=255)
    cGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagcc                                                                                                                                            >  1:2320513/1‑148 (MQ=255)
      tCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccac                                                                                                                                          >  2:736563/1‑148 (MQ=255)
         ggATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCcaccac                                                                                                                                       >  1:2852549/1‑148 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                      <  2:2039556/148‑1 (MQ=255)
           aTTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGt                                                                                                                                     >  2:2258742/1‑148 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                    <  2:1862515/148‑1 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                    <  2:3307665/148‑1 (MQ=255)
             ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                   >  2:2046435/1‑148 (MQ=255)
                     ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATc                                                                                                                           <  2:1719076/148‑1 (MQ=255)
                       ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTg                                                                                                                         >  2:2627123/1‑148 (MQ=255)
                         aTCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCa                                                                                                                       <  2:3365548/148‑1 (MQ=255)
                         aTCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCa                                                                                                                       >  1:2948638/1‑148 (MQ=255)
                           cTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAc                                                                                                                     >  1:63441/1‑148 (MQ=255)
                            ttCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACt                                                                                                                    <  1:359665/148‑1 (MQ=255)
                             tCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                   >  2:488474/1‑148 (MQ=255)
                               aCCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTcc                                                                                                                 <  2:756335/148‑1 (MQ=255)
                                cccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCg                                                                                                                >  2:2924315/1‑148 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                             <  2:3080218/148‑1 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                             <  1:3150972/148‑1 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                             >  1:1003386/1‑148 (MQ=255)
                                    gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                                                            <  2:623565/148‑1 (MQ=255)
                                    gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                                                            >  1:1322229/1‑148 (MQ=255)
                                    gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                                                            >  1:1322341/1‑148 (MQ=255)
                                         gAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                                                       >  1:1557878/1‑148 (MQ=255)
                                          aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                      >  2:1631112/1‑148 (MQ=255)
                                           ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACa                                                                                                     >  2:2668900/1‑148 (MQ=255)
                                               ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                                                                 >  1:3505182/1‑148 (MQ=255)
                                                    tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                            <  1:3121733/148‑1 (MQ=255)
                                                    tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                            <  1:2657695/148‑1 (MQ=255)
                                                     gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                           >  1:366825/1‑148 (MQ=255)
                                                          ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTg                                                                                      <  1:3114267/148‑1 (MQ=255)
                                                           gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                     <  1:2875083/148‑1 (MQ=255)
                                                           gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                     >  1:1001091/1‑148 (MQ=255)
                                                            aGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                    >  1:292679/1‑148 (MQ=255)
                                                             gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                                   <  1:364379/148‑1 (MQ=255)
                                                             gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                                   <  1:2357095/148‑1 (MQ=255)
                                                              tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                  >  2:124702/1‑148 (MQ=255)
                                                              tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                  >  2:2487483/1‑148 (MQ=255)
                                                                    gTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                                                            >  1:1215472/1‑148 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                        <  2:844007/148‑1 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                        <  2:399662/148‑1 (MQ=255)
                                                                          aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                      <  1:2513336/148‑1 (MQ=255)
                                                                          aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                      <  1:1069995/148‑1 (MQ=255)
                                                                           gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                                                     <  2:2137365/148‑1 (MQ=255)
                                                                              ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                                                                  <  2:317779/148‑1 (MQ=255)
                                                                              ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                                                                  >  2:453522/1‑148 (MQ=255)
                                                                              ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                                                                  >  2:2738244/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                 >  2:1497339/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                 >  2:1497867/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                 >  1:1407829/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGCTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                 >  1:1477577/1‑148 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                <  2:164615/148‑1 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                >  2:255064/1‑148 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                <  2:1351226/148‑1 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                >  2:185332/1‑148 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGATTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                <  2:2868839/148‑1 (MQ=255)
                                                                                  gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCg                                                              <  2:811028/148‑1 (MQ=255)
                                                                                  gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCg                                                              <  2:1921024/148‑1 (MQ=255)
                                                                                  gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCATACCCTGGCCATACGGGCTTGGAACTCCAGCCACCACGTTACGGATATCTGCAACTTCCGCGGTAGCGACATCGCGATCCCCGTTCATGCCGAAGTAGTGTATAGCATCg                                                              >  1:2488149/1‑148 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                             <  1:2730269/148‑1 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                             <  1:2730327/148‑1 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                             <  1:3461831/148‑1 (MQ=255)
                                                                                        aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                        >  2:524064/1‑148 (MQ=255)
                                                                                        aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                        >  2:494648/1‑148 (MQ=255)
                                                                                         ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                                                       <  2:3406175/148‑1 (MQ=255)
                                                                                             cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                   <  2:1642294/148‑1 (MQ=255)
                                                                                              ttCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACca                                                  >  2:65756/1‑148 (MQ=255)
                                                                                                cGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagc                                                <  2:720987/148‑1 (MQ=255)
                                                                                                   tACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                             >  2:1212713/1‑148 (MQ=255)
                                                                                                   tACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                             <  1:987999/148‑1 (MQ=255)
                                                                                                   tACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                             <  2:3515214/148‑1 (MQ=255)
                                                                                                      cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaa                                          <  2:2916603/148‑1 (MQ=255)
                                                                                                       aCGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTNCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                         >  1:1785309/1‑148 (MQ=255)
                                                                                                         gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATc                                       <  2:2292834/148‑1 (MQ=255)
                                                                                                          tttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCa                                      <  2:2732402/148‑1 (MQ=255)
                                                                                                           ttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                     >  1:847845/1‑148 (MQ=255)
                                                                                                             gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                   <  1:2649105/148‑1 (MQ=255)
                                                                                                             gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                   <  2:3251684/148‑1 (MQ=255)
                                                                                                              aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCa                                  >  1:1559137/1‑148 (MQ=255)
                                                                                                               tCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAt                                 <  2:15095/148‑1 (MQ=255)
                                                                                                               tCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAt                                 <  2:1186780/148‑1 (MQ=255)
                                                                                                                cagcagCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATc                                >  1:1915401/1‑148 (MQ=255)
                                                                                                                cagcagCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATc                                >  1:1631287/1‑148 (MQ=255)
                                                                                                                   cagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgcc                             >  2:1825216/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                          <  1:2738244/148‑1 (MQ=255)
                                                                                                                       cTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCaa                         <  2:944797/148‑1 (MQ=255)
                                                                                                                              ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                  >  2:1703788/1‑148 (MQ=255)
                                                                                                                              ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                  >  1:444225/1‑148 (MQ=255)
                                                                                                                                ccATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacggt                <  2:3362742/148‑1 (MQ=255)
                                                                                                                                 cATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTc               <  2:2186174/148‑1 (MQ=255)
                                                                                                                                   tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCaa             >  2:3192636/1‑148 (MQ=255)
                                                                                                                                    ccGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt            >  2:1138688/1‑148 (MQ=255)
                                                                                                                                       ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcac         <  1:458837/148‑1 (MQ=255)
                                                                                                                                         cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca       >  2:1416966/1‑148 (MQ=255)
                                                                                                                                         cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca       <  1:488474/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGA  >  CP009273/213939‑214228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑