Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,499,568 C→A 100% D182Y (GAC→TAC)  ypdF ← Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,499,5680CA98.2% 538.7 / 0.7 171D182Y (GAC→TAC) ypdFXaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (75/93);  total (78/93)
Fisher's exact test for biased strand distribution p-value = 9.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

ACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCA  >  CP009273/2499423‑2499714
                                                                                                                                                 |                                                                                                                                                  
aCCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTaaa                                                                                                                                                  >  1:1617102/1‑148 (MQ=255)
   aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAag                                                                                                                                               <  2:3384987/148‑1 (MQ=255)
   aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAag                                                                                                                                               <  2:2184201/148‑1 (MQ=255)
     ccaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAACTCGAGAGTGACAAACTCGCCCGCTGCAACACTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             <  1:3509197/146‑1 (MQ=255)
     cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             >  2:457412/1‑148 (MQ=255)
     cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             <  1:1497919/148‑1 (MQ=255)
     cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             <  2:1203781/148‑1 (MQ=255)
     cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             >  2:1066582/1‑148 (MQ=255)
     cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             >  1:3514719/1‑148 (MQ=255)
      aaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  2:746814/148‑1 (MQ=255)
      aaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  1:1848069/148‑1 (MQ=255)
      aaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  1:3003342/148‑1 (MQ=255)
      aaGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  1:3003213/148‑1 (MQ=255)
       aGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGc                                                                                                                                           >  1:1126808/1‑148 (MQ=255)
       aGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGc                                                                                                                                           <  1:3217885/148‑1 (MQ=255)
        ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCt                                                                                                                                          >  2:1371525/1‑148 (MQ=255)
        ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCt                                                                                                                                          <  2:1353719/148‑1 (MQ=255)
        ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCt                                                                                                                                          <  1:525632/148‑1 (MQ=255)
          tGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttt                                                                                                                                        <  1:3122986/148‑1 (MQ=255)
               gTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTg                                                                                                                                   <  2:238390/148‑1 (MQ=255)
                tCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgc                                                                                                                                  >  2:2022147/1‑148 (MQ=255)
                 cATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgcg                                                                                                                                 >  2:1112785/1‑148 (MQ=255)
                 cATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgcg                                                                                                                                 <  1:957940/148‑1 (MQ=255)
                   tatCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCGTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAACGAGGCTTTTTCTGCGcc                                                                                                                               <  2:3116520/148‑1 (MQ=255)
                    atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                              <  1:3297662/148‑1 (MQ=255)
                    atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                              <  1:5926/148‑1 (MQ=255)
                     tCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCct                                                                                                                             >  1:661836/1‑148 (MQ=255)
                      cAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                            >  2:231289/1‑148 (MQ=255)
                         agCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgctg                                                                                                                         >  1:2434007/1‑148 (MQ=255)
                          gCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTgg                                                                                                                        >  1:646054/1‑148 (MQ=255)
                              tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                    >  1:1363185/1‑148 (MQ=255)
                              tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                    <  1:2594499/148‑1 (MQ=255)
                                gCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTACCATGAGGCTTTTTCTGCGCCCTGCTGGCGCATa                                                                                                                  >  2:2848573/1‑148 (MQ=255)
                                 cccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                                 <  1:269284/148‑1 (MQ=255)
                                 cccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                                 >  1:559593/1‑148 (MQ=255)
                                     gATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCa                                                                                                             <  2:887024/148‑1 (MQ=255)
                                     gATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCa                                                                                                             <  2:292677/148‑1 (MQ=255)
                                      aTACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCAc                                                                                                            >  1:1745183/1‑148 (MQ=255)
                                       tACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACt                                                                                                           >  2:3297661/1‑148 (MQ=255)
                                       tACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACt                                                                                                           >  2:3297756/1‑148 (MQ=255)
                                         cAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTcc                                                                                                         <  2:3056768/148‑1 (MQ=255)
                                              cACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTc                                                                                                    >  2:2245366/1‑148 (MQ=255)
                                                ccGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                                  >  1:3424178/1‑148 (MQ=255)
                                                ccGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                                  >  1:1845623/1‑148 (MQ=255)
                                                     aTCGAGAGTGACACACTCGCCCGCTGCAACAATCGTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                             <  2:2152328/148‑1 (MQ=255)
                                                      tCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                            >  1:1943978/1‑148 (MQ=255)
                                                      tCGAGAGTGAAAAACTCGCCCGCTGCAAAAATCTTGGCGCTGGCTTGGCCGGGCGGCAGCGCCCCACGCCAGCCAGGGGCGAAAATGGTGAAAAAAGAGGCTTTTTCTGCCCCCGGCTGGCGAATAAACCACTCCAGTTCAGCGGCTa                                                                                            <  1:309988/148‑1 (MQ=255)
                                                       cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                           >  1:1321417/1‑148 (MQ=255)
                                                           agTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTcg                                                                                       >  1:1462644/1‑148 (MQ=255)
                                                              gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCgcgc                                                                                    <  2:795522/148‑1 (MQ=255)
                                                                    cTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCgctcgctc                                                                              >  2:2050837/1‑148 (MQ=255)
                                                                    cTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCgctcgctc                                                                              >  2:279855/1‑148 (MQ=255)
                                                                       gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATc                                                                           <  1:3120383/148‑1 (MQ=255)
                                                                       gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATc                                                                           <  2:3004935/148‑1 (MQ=255)
                                                                        cccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCCGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcc                                                                          <  1:1798332/148‑1 (MQ=255)
                                                                        cccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcc                                                                          >  2:1557984/1‑148 (MQ=255)
                                                                        cccGCTGCAACAATCTTGTCGCGGGCTTGGCCGTGCGGCAGCGCCCCACGCCCGCCACTGGCGACAATGGTGTAAAAAGCGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcc                                                                          <  1:21465/148‑1 (MQ=255)
                                                                          cGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcccc                                                                        >  2:1597743/1‑148 (MQ=255)
                                                                          cGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcccc                                                                        >  2:1598990/1‑148 (MQ=255)
                                                                            cTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGc                                                                      <  2:439094/148‑1 (MQ=255)
                                                                              gCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCt                                                                    >  1:3405643/1‑148 (MQ=255)
                                                                                aacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGa                                                                  >  2:249361/1‑148 (MQ=255)
                                                                                 acaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGaa                                                                 >  1:1977569/1‑148 (MQ=255)
                                                                                     tCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGACTaaa                                                             >  2:496171/1‑148 (MQ=255)
                                                                                      cTTGTCGCTGGCGTTGCCGTGCGGCAGCGCCCCACGCCAGCCACGGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACACCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCCCCTGAATaaac                                                            >  1:2359715/1‑147 (MQ=255)
                                                                                           cGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgcggcg                                                       >  1:769572/1‑148 (MQ=255)
                                                                                             cTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                     <  2:3026489/148‑1 (MQ=255)
                                                                                              tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAat                                                    <  2:2567242/148‑1 (MQ=255)
                                                                                              tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAat                                                    <  1:210325/148‑1 (MQ=255)
                                                                                               ggCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGActa                                                   >  2:3499870/1‑148 (MQ=255)
                                                                                               ggCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAata                                                   >  2:3413046/1‑148 (MQ=255)
                                                                                                gCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAatat                                                  <  2:1079863/148‑1 (MQ=255)
                                                                                                 cTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATg                                                 >  1:2498976/1‑148 (MQ=255)
                                                                                                 cTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATg                                                 >  1:2629217/1‑148 (MQ=255)
                                                                                                  tttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGc                                                >  1:488581/1‑148 (MQ=255)
                                                                                                   ttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAACGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGct                                               <  2:3393446/148‑1 (MQ=255)
                                                                                                    tGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctc                                              <  2:701792/148‑1 (MQ=255)
                                                                                                    tGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCGGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctc                                              <  1:2247803/148‑1 (MQ=255)
                                                                                                      ccGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTg                                            <  2:1126808/148‑1 (MQ=255)
                                                                                                             gCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACcgcg                                     >  1:922424/1‑148 (MQ=255)
                                                                                                               aGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGAt                                   <  2:3383041/148‑1 (MQ=255)
                                                                                                                   ccccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGc                               >  1:1055590/1‑148 (MQ=255)
                                                                                                                    cccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCa                              <  2:767777/148‑1 (MQ=255)
                                                                                                                    cccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCa                              <  2:2351585/148‑1 (MQ=255)
                                                                                                                       aCGCCTGACACTTGGGAAATTGGTTTAAAACTAGGCTTTTTATGCGCCCGGGTGGGGAATAAACCACTCCAGTTCAGCGGCTTTCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATc                           <  1:2169587/148‑1 (MQ=255)
                                                                                                                        cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                          <  1:279855/148‑1 (MQ=255)
                                                                                                                        cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                          <  1:2050837/148‑1 (MQ=255)
                                                                                                                            agccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                      <  2:191743/148‑1 (MQ=255)
                                                                                                                             gccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     >  1:2113613/1‑148 (MQ=255)
                                                                                                                              ccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACagg                    <  2:1874820/148‑1 (MQ=255)
                                                                                                                                aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcg                  <  2:568813/148‑1 (MQ=255)
                                                                                                                                  tGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag                >  1:3028807/1‑148 (MQ=255)
                                                                                                                                      gACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCgg            <  1:2713160/148‑1 (MQ=255)
                                                                                                                                        cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt          <  2:65740/148‑1 (MQ=255)
                                                                                                                                        cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAt          >  1:2839057/1‑148 (MQ=255)
                                                                                                                                           tGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtttt       >  2:609092/1‑148 (MQ=255)
                                                                                                                                           tGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtttt       <  1:2502345/148‑1 (MQ=255)
                                                                                                                                             gtgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCACACAGGCGAGGCGGATTTtct     >  2:1532451/1‑148 (MQ=255)
                                                                                                                                              tgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc    <  2:1637582/148‑1 (MQ=255)
                                                                                                                                                tCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCa  >  2:614412/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCA  >  CP009273/2499423‑2499714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑