Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,513,935 A→T 100% Y73N (TAC→AAC)  gltX ← glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,513,9350AT97.5% 642.3 / 4.5 198Y73N (TAC→AAC) gltXglutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base T (125/68);  total (128/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

TAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTACGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAGT  >  CP009273/2513790‑2514078
                                                                                                                                                 |                                                                                                                                               
tAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCg                                                                                                                                               <  1:1520884/148‑1 (MQ=255)
   cgcgGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGAc                                                                                                                                            >  2:1296101/1‑148 (MQ=255)
           ctcACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATcc                                                                                                                                    >  1:1736440/1‑148 (MQ=255)
            tcACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATccc                                                                                                                                   >  2:443427/1‑148 (MQ=255)
             cACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCa                                                                                                                                  >  2:171640/1‑148 (MQ=255)
             cACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCa                                                                                                                                  >  1:2052236/1‑148 (MQ=255)
             cACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCa                                                                                                                                  >  2:3174286/1‑148 (MQ=255)
              aCCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTACGGACCTTCATCCCAc                                                                                                                                 >  1:1402372/1‑148 (MQ=255)
                cTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTc                                                                                                                               >  2:431723/1‑148 (MQ=255)
                  ttCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCa                                                                                                                             <  1:1481309/148‑1 (MQ=255)
                  ttCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCa                                                                                                                             >  1:722076/1‑148 (MQ=255)
                      ccATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCt                                                                                                                         >  2:188127/1‑148 (MQ=255)
                         tttGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAg                                                                                                                      >  1:475901/1‑148 (MQ=255)
                          ttGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGc                                                                                                                     >  1:2476961/1‑148 (MQ=255)
                          ttGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGc                                                                                                                     >  2:3452190/1‑148 (MQ=255)
                          ttGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGc                                                                                                                     >  2:1156296/1‑148 (MQ=255)
                            gCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTACGGACCTTCATCCCACTCCAGGCTCAGCCa                                                                                                                   >  1:2419110/1‑148 (MQ=255)
                                ttCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTc                                                                                                               >  2:1349948/1‑148 (MQ=255)
                                ttCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTc                                                                                                               <  1:3002926/148‑1 (MQ=255)
                                      cAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGcca                                                                                                         >  1:2621440/1‑148 (MQ=255)
                                      cAGCGCTTCCAGGCGCTCGTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCACACTCCAGGCTCAGCCAGTTCATGcca                                                                                                         >  1:766801/1‑148 (MQ=255)
                                             tCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATaa                                                                                                  <  1:1806846/148‑1 (MQ=255)
                                             tCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATaa                                                                                                  >  1:3335753/1‑148 (MQ=255)
                                              ccAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAAt                                                                                                 <  1:3510868/148‑1 (MQ=255)
                                                aGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATgg                                                                                               <  2:2052236/148‑1 (MQ=255)
                                                aGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATgg                                                                                               <  1:590488/148‑1 (MQ=255)
                                                      tctTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGa                                                                                         <  2:2700489/148‑1 (MQ=255)
                                                      tctTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTAATGCCATCCATAATGGCTTCGa                                                                                         <  1:2422654/148‑1 (MQ=255)
                                                       ctTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGAt                                                                                        >  2:2401829/1‑148 (MQ=255)
                                                       ctTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGAt                                                                                        >  2:310836/1‑148 (MQ=255)
                                                        tttAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATa                                                                                       <  1:1701446/148‑1 (MQ=255)
                                                         ttAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAg                                                                                      <  2:2835234/148‑1 (MQ=255)
                                                          tAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGc                                                                                     <  1:1080578/148‑1 (MQ=255)
                                                           agagCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCt                                                                                    >  1:2973912/1‑148 (MQ=255)
                                                             agCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTc                                                                                  >  1:910643/1‑148 (MQ=255)
                                                              gCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTATGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTcc                                                                                 >  1:2398065/1‑148 (MQ=255)
                                                               cAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCg                                                                                >  2:2084784/1‑148 (MQ=255)
                                                               cAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCg                                                                                <  1:1238946/148‑1 (MQ=255)
                                                               cAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTACGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCg                                                                                >  2:465452/1‑148 (MQ=255)
                                                                 aTAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGc                                                                              >  1:1217750/1‑148 (MQ=255)
                                                                 aTAGCATTTATAAGCAGTGCCCTCTTCCAGAATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCAACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGATTCGATAGAATCCGGc                                                                              >  2:2141048/1‑148 (MQ=255)
                                                                  tAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCg                                                                             >  1:1922686/1‑148 (MQ=255)
                                                                   aGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGt                                                                            >  1:643402/1‑148 (MQ=255)
                                                                       tttATAAGCAGGGCCCTCGTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGaa                                                                        <  2:2564722/148‑1 (MQ=255)
                                                                        ttATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAAc                                                                       >  2:1890396/1‑148 (MQ=255)
                                                                         tataAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACg                                                                      >  1:3393879/1‑148 (MQ=255)
                                                                            aaGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTc                                                                   >  2:2122535/1‑148 (MQ=255)
                                                                             aGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCa                                                                  >  1:958264/1‑148 (MQ=255)
                                                                               cAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAg                                                                >  2:327665/1‑148 (MQ=255)
                                                                               cAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAg                                                                >  2:149881/1‑148 (MQ=255)
                                                                               cAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAg                                                                >  1:3233689/1‑148 (MQ=255)
                                                                                 gTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGAt                                                              >  1:1379103/1‑148 (MQ=255)
                                                                                    cccTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCgg                                                           <  1:100067/148‑1 (MQ=255)
                                                                                    cccTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCgg                                                           >  1:1931655/1‑148 (MQ=255)
                                                                                       tctTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGgtgt                                                        <  2:3482487/148‑1 (MQ=255)
                                                                                       tctTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGgtgt                                                        >  2:13773/1‑148 (MQ=255)
                                                                                          tCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCtt                                                     >  2:2085204/1‑148 (MQ=255)
                                                                                            cAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCa                                                   >  1:2696564/1‑148 (MQ=255)
                                                                                               cATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCTGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATa                                                <  1:2508910/148‑1 (MQ=255)
                                                                                               cATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATa                                                >  1:1347326/1‑148 (MQ=255)
                                                                                               cATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATa                                                >  1:2031838/1‑148 (MQ=255)
                                                                                               cATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATa                                                >  1:3461266/1‑148 (MQ=255)
                                                                                                 tCTGATCGATCACCGCGTTGTAGCGATCAAATCGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCATCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACg                                              <  2:645466/148‑1 (MQ=255)
                                                                                                 tCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACg                                              <  2:1876094/148‑1 (MQ=255)
                                                                                                  cTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgc                                             >  2:387756/1‑148 (MQ=255)
                                                                                                  cTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgc                                             >  1:1600943/1‑148 (MQ=255)
                                                                                                   tGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgca                                            <  1:443427/148‑1 (MQ=255)
                                                                                                   tGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgca                                            >  1:1143661/1‑148 (MQ=255)
                                                                                                     atcgatcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgcagc                                          >  1:266490/1‑148 (MQ=255)
                                                                                                     atcgatcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgcagc                                          <  2:2037027/148‑1 (MQ=255)
                                                                                                     atcgatcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACgcagc                                          <  2:2038472/148‑1 (MQ=255)
                                                                                                       cgatcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCAc                                        >  1:1267267/1‑148 (MQ=255)
                                                                                                         atcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGa                                      >  2:2672552/1‑148 (MQ=255)
                                                                                                          tcACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGaa                                     >  1:2811658/1‑148 (MQ=255)
                                                                                                           cACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAAc                                    <  1:1646788/148‑1 (MQ=255)
                                                                                                           cACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAAc                                    >  2:2293285/1‑148 (MQ=255)
                                                                                                            aCCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACt                                   <  1:2313/148‑1 (MQ=255)
                                                                                                             ccGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTc                                  >  2:2942168/1‑148 (MQ=255)
                                                                                                              cgcgTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCa                                 >  1:2569534/1‑148 (MQ=255)
                                                                                                                cgTTGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCAcc                               >  2:1382589/1‑148 (MQ=255)
                                                                                                                  ttGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCAccgc                             >  1:2551745/1‑148 (MQ=255)
                                                                                                                   tGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCAccgcc                            >  2:223972/1‑148 (MQ=255)
                                                                                                                   tGTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCAccgcc                            >  2:1270514/1‑148 (MQ=255)
                                                                                                                    gTAGCGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCAccgccg                           <  2:1084941/148‑1 (MQ=255)
                                                                                                                       gCGATCAACACGATTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTgg                        <  1:2855182/148‑1 (MQ=255)
                                                                                                                        cGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCAGGGGGGGCTCAGCGGGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGt                       >  1:1609666/1‑148 (MQ=255)
                                                                                                                        cGATCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGt                       >  2:2986654/1‑148 (MQ=255)
                                                                                                                          aTCAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTa                     >  2:1537409/1‑148 (MQ=255)
                                                                                                                            cAAAACGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACg                   >  1:1289573/1‑148 (MQ=255)
                                                                                                                              aaaCGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTg                 >  2:738530/1‑148 (MQ=255)
                                                                                                                               aaCGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGc                >  2:3025802/1‑148 (MQ=255)
                                                                                                                               aaCGTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGc                >  2:2550786/1‑148 (MQ=255)
                                                                                                                                  gTTTGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCaaa             >  1:309922/1‑148 (MQ=255)
                                                                                                                                   tttGGTCTGGTAGTTCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCaaaa            <  1:431694/148‑1 (MQ=255)
                                                                                                                                            gtagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGTCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAg   >  2:1175599/1‑148 (MQ=255)
                                                                                                                                            gtagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAg   <  2:1217750/148‑1 (MQ=255)
                                                                                                                                            gtagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAg   <  2:1596673/148‑1 (MQ=255)
                                                                                                                                             tagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAGt  >  1:2179712/1‑148 (MQ=255)
                                                                                                                                             tagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAGt  >  2:2457909/1‑148 (MQ=255)
                                                                                                                                             tagttCGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAGt  >  2:2340555/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTTTGGTCTGGTAGTACGGACCTTCATCCCACTCCAGGCTCAGCCAGTTCATGCCATCCATAATGGCTTCGATAGCTTCCGGCGTGGAACGCTCAAGATCGGTGTCTTCAATACGCAGCACGAACTCACCGCCGTGGTTACGTGCAAAAAGCCAGGAGT  >  CP009273/2513790‑2514078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑