Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,549,676 C→A 100% L28L (CTG→CTT)  eutL ← putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,549,6760CA99.6% 743.5 / ‑4.3 225L28L (CTG→CTT) eutLputative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (114/110);  total (114/111)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

GGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCC  >  CP009273/2549531‑2549820
                                                                                                                                                 |                                                                                                                                                
ggCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCACTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAg                                                                                                                                                >  2:972580/1‑148 (MQ=255)
  ccGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGtt                                                                                                                                              <  2:1022453/148‑1 (MQ=255)
    gtgtGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                            <  1:2380848/148‑1 (MQ=255)
      gtgCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAcg                                                                                                                                          >  1:210100/1‑148 (MQ=255)
           gcgcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCaa                                                                                                                                     <  1:1840007/148‑1 (MQ=255)
             gcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCaaaa                                                                                                                                   <  1:97132/148‑1 (MQ=255)
             gcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCaaaa                                                                                                                                   <  1:1339492/148‑1 (MQ=255)
              cccGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAAt                                                                                                                                  <  1:2252790/148‑1 (MQ=255)
                  cATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGc                                                                                                                              >  1:2567704/1‑148 (MQ=255)
                    tataGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGt                                                                                                                            >  2:3096968/1‑148 (MQ=255)
                       agcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTaa                                                                                                                         <  1:279119/148‑1 (MQ=255)
                        gcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAAc                                                                                                                        <  1:3499623/148‑1 (MQ=255)
                        gcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAAc                                                                                                                        <  1:1032920/148‑1 (MQ=255)
                        gcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAAc                                                                                                                        <  1:2060231/148‑1 (MQ=255)
                        gcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAAc                                                                                                                        <  1:2494232/148‑1 (MQ=255)
                                   tACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCCAAATCGGCGTTAACGGAGGCACTCa                                                                                                             >  1:2843956/1‑148 (MQ=255)
                                   tACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCa                                                                                                             <  1:1045122/148‑1 (MQ=255)
                                    acacCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAc                                                                                                            >  1:717187/1‑148 (MQ=255)
                                    acacCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAc                                                                                                            <  2:755871/148‑1 (MQ=255)
                                     caccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAcc                                                                                                           <  2:518151/148‑1 (MQ=255)
                                           ttCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATa                                                                                                     <  1:1176286/148‑1 (MQ=255)
                                            tCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAg                                                                                                    <  1:1673242/148‑1 (MQ=255)
                                              caccaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCg                                                                                                  <  1:667203/148‑1 (MQ=255)
                                               accaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCgg                                                                                                 >  2:2939528/1‑148 (MQ=255)
                                               accaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCgg                                                                                                 >  2:2939724/1‑148 (MQ=255)
                                               accaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCgg                                                                                                 >  2:1850537/1‑148 (MQ=255)
                                                  aTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGa                                                                                              >  2:2297828/1‑148 (MQ=255)
                                                   tCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGAc                                                                                             >  1:922697/1‑148 (MQ=255)
                                                     gCCTGCGTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAg                                                                                           <  2:211634/148‑1 (MQ=255)
                                                      ccTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGa                                                                                          >  2:1945532/1‑148 (MQ=255)
                                                        tGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACg                                                                                        >  1:3321144/1‑148 (MQ=255)
                                                         gCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACgg                                                                                       <  2:666918/148‑1 (MQ=255)
                                                          cTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGt                                                                                      <  1:1467378/148‑1 (MQ=255)
                                                          cTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGt                                                                                      <  1:2632390/148‑1 (MQ=255)
                                                             ggTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGa                                                                                   >  2:2495226/1‑148 (MQ=255)
                                                             ggTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGa                                                                                   <  2:159403/148‑1 (MQ=255)
                                                              gTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGaa                                                                                  <  1:995705/148‑1 (MQ=255)
                                                                  cTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaa                                                                              <  2:1209281/148‑1 (MQ=255)
                                                                   ttCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaaa                                                                             <  2:2554829/148‑1 (MQ=255)
                                                                   ttCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaaa                                                                             <  1:2733557/148‑1 (MQ=255)
                                                                    tcatcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAAt                                                                            >  2:2221817/1‑148 (MQ=255)
                                                                    tcatcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAAt                                                                            >  2:569380/1‑148 (MQ=255)
                                                                    tcatcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAAt                                                                            >  1:1913991/1‑148 (MQ=255)
                                                                        caGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaa                                                                        >  1:2968872/1‑148 (MQ=255)
                                                                          gCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAg                                                                      <  2:3190968/148‑1 (MQ=255)
                                                                           cGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGc                                                                     >  2:1668069/1‑148 (MQ=255)
                                                                           cGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGc                                                                     <  1:2187409/148‑1 (MQ=255)
                                                                           cGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGc                                                                     >  2:2449164/1‑148 (MQ=255)
                                                                           cGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAAGCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCACATCTAAAGc                                                                     >  2:2649005/1‑148 (MQ=255)
                                                                             gCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTg                                                                   <  1:1437408/148‑1 (MQ=255)
                                                                              cAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTgg                                                                  <  1:2596599/148‑1 (MQ=255)
                                                                                  gTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatg                                                              >  1:45671/1‑148 (MQ=255)
                                                                                  gTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatg                                                              <  1:2998053/148‑1 (MQ=255)
                                                                                     ggTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatgatg                                                           >  2:460429/1‑148 (MQ=255)
                                                                                       tGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcc                                                         >  2:3488909/1‑148 (MQ=255)
                                                                                        gACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcct                                                        <  2:2604263/148‑1 (MQ=255)
                                                                                         aCGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctc                                                       <  2:717187/148‑1 (MQ=255)
                                                                                          cGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcc                                                      <  1:1661771/148‑1 (MQ=255)
                                                                                          cGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcc                                                      <  2:2414877/148‑1 (MQ=255)
                                                                                           gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcct                                                     <  2:1568941/148‑1 (MQ=255)
                                                                                           gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcct                                                     <  2:1569057/148‑1 (MQ=255)
                                                                                             cATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                   <  2:3449445/148‑1 (MQ=255)
                                                                                             cATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                   >  2:3423214/1‑148 (MQ=255)
                                                                                              aTCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTAt                                                  <  2:597363/148‑1 (MQ=255)
                                                                                                cGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCg                                                >  1:394837/1‑148 (MQ=255)
                                                                                                   aGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGt                                             >  1:1093734/1‑148 (MQ=255)
                                                                                                    gTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTc                                            <  1:3156093/148‑1 (MQ=255)
                                                                                                     tCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCa                                           <  1:1945563/148‑1 (MQ=255)
                                                                                                        gCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGt                                        <  1:766333/148‑1 (MQ=255)
                                                                                                          aGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTg                                      <  2:2817874/148‑1 (MQ=255)
                                                                                                          aGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTg                                      <  2:899066/148‑1 (MQ=255)
                                                                                                           gAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGa                                     >  2:174493/1‑148 (MQ=255)
                                                                                                           gAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGa                                     <  2:2541954/148‑1 (MQ=255)
                                                                                                           gAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGa                                     <  2:2567704/148‑1 (MQ=255)
                                                                                                              aTCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGc                                  >  2:2052884/1‑148 (MQ=255)
                                                                                                               tCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGcc                                 >  2:1415616/1‑148 (MQ=255)
                                                                                                                  gACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGa                              >  1:1063417/1‑148 (MQ=255)
                                                                                                                   aCCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                             >  1:2308405/1‑148 (MQ=255)
                                                                                                                    ccGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATg                            <  1:2390400/148‑1 (MQ=255)
                                                                                                                     cGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGc                           <  1:2838300/148‑1 (MQ=255)
                                                                                                                       aGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCtt                         >  1:318870/1‑148 (MQ=255)
                                                                                                                       aGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCtt                         >  1:2657301/1‑148 (MQ=255)
                                                                                                                           tACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTg                     <  2:69673/148‑1 (MQ=255)
                                                                                                                            aCGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGc                    <  1:2177566/148‑1 (MQ=255)
                                                                                                                            aCGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGc                    >  1:3133613/1‑148 (MQ=255)
                                                                                                                             cGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCt                   <  1:972580/148‑1 (MQ=255)
                                                                                                                                atatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCa                >  1:3040929/1‑148 (MQ=255)
                                                                                                                                 tatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAg               >  2:1757348/1‑148 (MQ=255)
                                                                                                                                  atGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGc              >  1:313565/1‑148 (MQ=255)
                                                                                                                                   tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCa             >  2:537567/1‑148 (MQ=255)
                                                                                                                                   tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCa             >  1:1954444/1‑148 (MQ=255)
                                                                                                                                   tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCa             >  1:3176705/1‑148 (MQ=255)
                                                                                                                                   tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCa             >  2:2303340/1‑148 (MQ=255)
                                                                                                                                    gcggcgGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCAt            <  2:1356619/148‑1 (MQ=255)
                                                                                                                                         ggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGtt       <  2:3264642/148‑1 (MQ=255)
                                                                                                                                             aTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGc   >  1:2159080/1‑148 (MQ=255)
                                                                                                                                              tttAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc  >  1:541632/1‑148 (MQ=255)
                                                                                                                                              tttAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc  >  1:3453642/1‑148 (MQ=255)
                                                                                                                                              tttAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc  >  2:923992/1‑148 (MQ=255)
                                                                                                                                              tttAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc  >  2:926144/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCC  >  CP009273/2549531‑2549820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑