Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,552,461 A→G 100% N82S (AAT→AGT)  intZ → CPZ‑55 prophage; putative phage integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,552,4610AG95.2% 770.2 / 16.1 206N82S (AAT→AGT) intZCPZ‑55 prophage; putative phage integrase
Reads supporting (aligned to +/- strand):  ref base A (4/6);  new base G (92/104);  total (96/110)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

CTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAT  >  CP009273/2552316‑2552604
                                                                                                                                                 |                                                                                                                                               
cTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTg                                                                                                                                               >  1:123464/1‑148 (MQ=255)
        tCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAc                                                                                                                                       <  2:179865/148‑1 (MQ=255)
         cccAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAcc                                                                                                                                      >  1:1214122/1‑148 (MQ=255)
         cccAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACACAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAcc                                                                                                                                      >  1:722409/1‑148 (MQ=255)
            aaCGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAg                                                                                                                                   <  1:923807/148‑1 (MQ=255)
                 ttCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGaa                                                                                                                              >  2:1405452/1‑148 (MQ=255)
                 ttCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGaa                                                                                                                              >  2:1018172/1‑148 (MQ=255)
                 ttCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGaa                                                                                                                              >  1:1096023/1‑148 (MQ=255)
                   cGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACa                                                                                                                            >  1:1003076/1‑148 (MQ=255)
                   cGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACa                                                                                                                            <  2:2219860/148‑1 (MQ=255)
                     aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAg                                                                                                                          >  2:2749446/1‑148 (MQ=255)
                     aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAg                                                                                                                          >  2:2160816/1‑148 (MQ=255)
                     aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAg                                                                                                                          >  2:1631490/1‑148 (MQ=255)
                     aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAg                                                                                                                          >  2:2804136/1‑148 (MQ=255)
                       aTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGcc                                                                                                                        >  1:127953/1‑148 (MQ=255)
                       aTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGcc                                                                                                                        <  2:1760138/148‑1 (MQ=255)
                        tACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGccc                                                                                                                       >  2:706791/1‑148 (MQ=255)
                        tACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGccc                                                                                                                       >  1:3361894/1‑148 (MQ=255)
                         aCTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCg                                                                                                                      >  1:2156557/1‑148 (MQ=255)
                           tGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCa                                                                                                                    <  1:3085295/148‑1 (MQ=255)
                            ggCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCaa                                                                                                                   <  1:1456608/148‑1 (MQ=255)
                             gCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAg                                                                                                                  >  1:2777386/1‑148 (MQ=255)
                                 ttCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGaaa                                                                                                              >  1:2992176/1‑148 (MQ=255)
                                  tCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGaaaa                                                                                                             >  1:1915749/1‑148 (MQ=255)
                                     ggTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGACTTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGc                                                                                                          >  2:1678053/1‑148 (MQ=255)
                                     ggTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGc                                                                                                          <  2:2396343/148‑1 (MQ=255)
                                       tATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCa                                                                                                        <  2:2666195/148‑1 (MQ=255)
                                       tATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCa                                                                                                        <  1:2026814/148‑1 (MQ=255)
                                          tGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCCAGGAAAAAGCCAGTg                                                                                                     >  2:509941/1‑148 (MQ=255)
                                          tGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCCTTg                                                                                                     >  2:1289654/1‑148 (MQ=255)
                                          tGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTg                                                                                                     >  2:1272519/1‑148 (MQ=255)
                                          tGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTg                                                                                                     <  1:2366657/148‑1 (MQ=255)
                                           ggCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGa                                                                                                    <  2:3308929/148‑1 (MQ=255)
                                            gCTGTATAAACGAGAGTTTTTGCAGGCCAGTGGCGTTTACCCGCTAATCACACTCAAAGTCGCACGCAGGCGCGCCACGGCGAGCCGATCCCTTATTGCCAGTGGACTTAACCCAGTGGACCTAGCCCGCACGGATATGGCCAGTGAt                                                                                                   >  2:1956363/1‑148 (MQ=255)
                                               gAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATgcg                                                                                                <  1:1631490/148‑1 (MQ=255)
                                               gAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATgcg                                                                                                <  2:2359897/148‑1 (MQ=255)
                                                  tAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTc                                                                                             >  1:3459790/1‑148 (MQ=255)
                                                   aaaCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCa                                                                                            >  1:2724720/1‑148 (MQ=255)
                                                     aCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAAc                                                                                          <  1:2552364/148‑1 (MQ=255)
                                                      cGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACa                                                                                         <  2:1134910/148‑1 (MQ=255)
                                                       gagaGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACAt                                                                                        <  1:3435406/148‑1 (MQ=255)
                                                       gagaGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACAt                                                                                        <  1:933888/148‑1 (MQ=255)
                                                         gagTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATgg                                                                                      >  1:2356931/1‑148 (MQ=255)
                                                          agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                     >  2:2890658/1‑148 (MQ=255)
                                                          agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                     >  1:1748548/1‑148 (MQ=255)
                                                           gTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcg                                                                                    >  1:2675835/1‑148 (MQ=255)
                                                             ttttGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcggc                                                                                  <  2:3198436/148‑1 (MQ=255)
                                                             ttttGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcggc                                                                                  <  2:3198855/148‑1 (MQ=255)
                                                             ttttGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcggc                                                                                  <  2:1096023/148‑1 (MQ=255)
                                                                 gCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGa                                                                              <  2:2950114/148‑1 (MQ=255)
                                                                 gCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCATTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGa                                                                              >  2:403214/1‑148 (MQ=255)
                                                                    ggCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGAttt                                                                           >  2:806868/1‑148 (MQ=255)
                                                                     gCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTa                                                                          >  2:2228921/1‑148 (MQ=255)
                                                                     gCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTa                                                                          <  2:2644613/148‑1 (MQ=255)
                                                                     gCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTa                                                                          >  2:2843727/1‑148 (MQ=255)
                                                                        aTTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaaga                                                                       <  1:49131/148‑1 (MQ=255)
                                                                        aTTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaaga                                                                       <  1:3163242/148‑1 (MQ=255)
                                                                        aTTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaaga                                                                       <  1:2471619/148‑1 (MQ=255)
                                                                          tGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaagaag                                                                     >  2:2267624/1‑148 (MQ=255)
                                                                            gAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGt                                                                   >  1:520800/1‑148 (MQ=255)
                                                                                     cGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGa                                                          <  1:953441/148‑1 (MQ=255)
                                                                                      gCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGAc                                                         <  2:1840354/148‑1 (MQ=255)
                                                                                        tAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTg                                                       <  1:1369891/148‑1 (MQ=255)
                                                                                        tAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTg                                                       <  1:254098/148‑1 (MQ=255)
                                                                                            cacaCTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGttt                                                   >  1:362697/1‑148 (MQ=255)
                                                                                             acacTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTg                                                  <  2:1579699/148‑1 (MQ=255)
                                                                                                 tCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTAc                                              >  1:2757034/1‑148 (MQ=255)
                                                                                                   aaaGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCa                                            <  1:838276/148‑1 (MQ=255)
                                                                                                   aaaGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCa                                            <  2:3365080/148‑1 (MQ=255)
                                                                                                   aaaGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCa                                            <  1:1845133/148‑1 (MQ=255)
                                                                                                      gaagCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAggg                                         <  1:1903865/148‑1 (MQ=255)
                                                                                                         gCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTg                                      >  1:682090/1‑148 (MQ=255)
                                                                                                          cACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTgg                                     <  2:520800/148‑1 (MQ=255)
                                                                                                            cGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAg                                   >  2:1689157/1‑148 (MQ=255)
                                                                                                            cGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAg                                   >  2:1689029/1‑148 (MQ=255)
                                                                                                              cAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAggg                                 <  1:3322668/148‑1 (MQ=255)
                                                                                                               aGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggt                                <  2:1552138/148‑1 (MQ=255)
                                                                                                                 gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                              <  2:2776792/148‑1 (MQ=255)
                                                                                                                 gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                              <  1:1076010/148‑1 (MQ=255)
                                                                                                                 gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                              <  2:1395894/148‑1 (MQ=255)
                                                                                                                 gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                              >  1:163506/1‑148 (MQ=255)
                                                                                                                 gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                              <  1:607655/148‑1 (MQ=255)
                                                                                                                  cgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtggt                             <  2:3281178/148‑1 (MQ=255)
                                                                                                                    cgcCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCg                           <  1:2224934/148‑1 (MQ=255)
                                                                                                                         cGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATc                      >  2:3073665/1‑148 (MQ=255)
                                                                                                                          ggAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATcc                     <  1:263616/148‑1 (MQ=255)
                                                                                                                            agagCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                   <  1:2774449/148‑1 (MQ=255)
                                                                                                                              agCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATg                 <  1:1177164/148‑1 (MQ=255)
                                                                                                                                  gATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaaa             <  2:729374/148‑1 (MQ=255)
                                                                                                                                   aTCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaaaa            <  2:928122/148‑1 (MQ=255)
                                                                                                                                   aTCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaaaa            >  2:2081656/1‑148 (MQ=255)
                                                                                                                                   aTCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaaaa            >  2:2914851/1‑148 (MQ=255)
                                                                                                                                    tCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc           <  2:1648953/148‑1 (MQ=255)
                                                                                                                                    tCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAAc           <  2:2023109/148‑1 (MQ=255)
                                                                                                                                      ccTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAg         <  2:3450361/148‑1 (MQ=255)
                                                                                                                                      ccTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAg         <  2:3013677/148‑1 (MQ=255)
                                                                                                                                       cTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAgg        <  1:706791/148‑1 (MQ=255)
                                                                                                                                       cTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAgg        >  1:1799850/1‑148 (MQ=255)
                                                                                                                                         tATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTc      >  1:3182499/1‑148 (MQ=255)
                                                                                                                                             gCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAt  <  2:1214122/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGAT  >  CP009273/2552316‑2552604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑