Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,211 C→A 100% D152Y (GAT→TAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,2110CA98.9% 565.1 / ‑1.7 178D152Y (GAT→TAT) eutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (96/80);  total (97/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

AGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGC  >  CP009273/2567066‑2567355
                                                                                                                                                 |                                                                                                                                                
aGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAgg                                                                                                                                                >  1:3156823/1‑148 (MQ=255)
 gCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgc                                                                                                                                               <  1:1589663/148‑1 (MQ=255)
    aCGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcg                                                                                                                                            <  1:299315/148‑1 (MQ=255)
     cGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcgc                                                                                                                                           <  1:3252266/148‑1 (MQ=255)
         gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATaa                                                                                                                                       <  1:2838284/148‑1 (MQ=255)
         gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATaa                                                                                                                                       <  2:2709923/148‑1 (MQ=255)
          caTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAAt                                                                                                                                      >  1:1717858/1‑148 (MQ=255)
          caTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAAt                                                                                                                                      >  1:1377627/1‑148 (MQ=255)
           aTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATg                                                                                                                                     <  2:1815247/148‑1 (MQ=255)
            tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGt                                                                                                                                    >  2:2916863/1‑148 (MQ=255)
             cgcgCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCTAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGtt                                                                                                                                   >  1:3043963/1‑148 (MQ=255)
                  ccGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCaa                                                                                                                              >  2:1205139/1‑148 (MQ=255)
                  ccGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCaa                                                                                                                              >  2:3293305/1‑148 (MQ=255)
                   cGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAg                                                                                                                             <  2:1336401/148‑1 (MQ=255)
                           gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                     >  1:1013476/1‑148 (MQ=255)
                           gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                     >  2:174105/1‑148 (MQ=255)
                           gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                     >  1:2913799/1‑148 (MQ=255)
                           gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                     >  1:1012914/1‑148 (MQ=255)
                            ctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACg                                                                                                                    <  2:1869623/148‑1 (MQ=255)
                              tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                  >  2:2352806/1‑148 (MQ=255)
                              tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                  >  2:3422650/1‑148 (MQ=255)
                                cGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGata                                                                                                                >  1:2246260/1‑148 (MQ=255)
                                   cACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATcc                                                                                                             >  1:1841200/1‑148 (MQ=255)
                                   cACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATcc                                                                                                             >  2:2229176/1‑148 (MQ=255)
                                    aCCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCg                                                                                                            >  2:2331381/1‑148 (MQ=255)
                                      cAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgcc                                                                                                          >  2:2147069/1‑148 (MQ=255)
                                      cAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgcc                                                                                                          >  2:2147153/1‑148 (MQ=255)
                                          tgatCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccagcc                                                                                                      >  1:3209871/1‑148 (MQ=255)
                                             tCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCCTAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACg                                                                                                   >  2:1043852/1‑148 (MQ=255)
                                             tCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACg                                                                                                   >  2:2625528/1‑148 (MQ=255)
                                                 ggTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTg                                                                                               <  2:2565152/148‑1 (MQ=255)
                                                        aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        >  1:2498213/1‑148 (MQ=255)
                                                        aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        >  1:942322/1‑148 (MQ=255)
                                                        aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        >  1:738383/1‑148 (MQ=255)
                                                         tAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGtt                                                                                       >  2:603780/1‑148 (MQ=255)
                                                            cgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCg                                                                                    >  2:81239/1‑148 (MQ=255)
                                                                 cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGt                                                                               >  1:3445367/1‑148 (MQ=255)
                                                                 cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGt                                                                               >  1:3445685/1‑148 (MQ=255)
                                                                  ggCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGtt                                                                              <  1:360209/148‑1 (MQ=255)
                                                                      gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAt                                                                          >  2:2833257/1‑148 (MQ=255)
                                                                         gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTg                                                                       >  1:2636929/1‑148 (MQ=255)
                                                                         gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTg                                                                       <  2:1340576/148‑1 (MQ=255)
                                                                         gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTg                                                                       >  1:1369659/1‑148 (MQ=255)
                                                                            aaaGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAg                                                                    >  2:140474/1‑148 (MQ=255)
                                                                                ccGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCcac                                                                >  1:1291686/1‑148 (MQ=255)
                                                                                     gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                           >  2:640186/1‑148 (MQ=255)
                                                                                     gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                           <  1:1812259/148‑1 (MQ=255)
                                                                                     gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                           >  2:3464173/1‑148 (MQ=255)
                                                                                      cAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTgtc                                                          >  1:3345392/1‑146 (MQ=255)
                                                                                       aGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCa                                                         >  1:102894/1‑148 (MQ=255)
                                                                                        gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                        <  1:2211926/148‑1 (MQ=255)
                                                                                        gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                        >  1:2390335/1‑148 (MQ=255)
                                                                                        gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                        >  1:2390769/1‑148 (MQ=255)
                                                                                         aTCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                       >  1:1808954/1‑148 (MQ=255)
                                                                                         aTCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                       >  1:1485017/1‑148 (MQ=255)
                                                                                         aTCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                       >  2:2237488/1‑148 (MQ=255)
                                                                                          tCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgcg                                                      >  2:1864693/1‑148 (MQ=255)
                                                                                            ttCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCa                                                    <  1:2916863/148‑1 (MQ=255)
                                                                                             tCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCaa                                                   >  2:799800/1‑148 (MQ=255)
                                                                                              cGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAAt                                                  <  1:2753160/148‑1 (MQ=255)
                                                                                                 cAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGt                                               >  1:1420022/1‑148 (MQ=255)
                                                                                                 cAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGt                                               >  2:889070/1‑148 (MQ=255)
                                                                                                  aGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                              <  1:2614315/148‑1 (MQ=255)
                                                                                                   gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGc                                             >  1:487994/1‑148 (MQ=255)
                                                                                                   gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGc                                             >  2:875485/1‑148 (MQ=255)
                                                                                                     aaGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTg                                           <  2:2718070/148‑1 (MQ=255)
                                                                                                      aGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGt                                          >  1:1886267/1‑148 (MQ=255)
                                                                                                      aGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGt                                          >  1:109857/1‑148 (MQ=255)
                                                                                                      aGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGt                                          >  2:1418187/1‑148 (MQ=255)
                                                                                                        cccGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCg                                        >  1:1520229/1‑148 (MQ=255)
                                                                                                        cccGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCg                                        <  2:557123/148‑1 (MQ=255)
                                                                                                         ccGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCga                                       <  1:1029958/148‑1 (MQ=255)
                                                                                                           gACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCgaga                                     <  2:262021/148‑1 (MQ=255)
                                                                                                            aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGaa                                    <  1:2520418/148‑1 (MQ=255)
                                                                                                            aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGaa                                    <  2:518803/148‑1 (MQ=255)
                                                                                                                  gCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgcgc                              <  1:640186/148‑1 (MQ=255)
                                                                                                                      ggCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaa                          <  1:140474/148‑1 (MQ=255)
                                                                                                                          gCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAccc                      <  2:203805/148‑1 (MQ=255)
                                                                                                                          gCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAccc                      <  2:190130/148‑1 (MQ=255)
                                                                                                                          gCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAAGCTAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCTCCAGTTCATTTTGCAGCCAAACTGCCATCGCAATGGTTCTGTCGAGAACAGATAGAAAAccc                      <  2:1717858/148‑1 (MQ=255)
                                                                                                                           cccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAACACCCa                     >  2:511499/1‑148 (MQ=255)
                                                                                                                            ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAg                    <  2:2123412/148‑1 (MQ=255)
                                                                                                                            ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAg                    <  2:2390769/148‑1 (MQ=255)
                                                                                                                             cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                   >  2:2851387/1‑148 (MQ=255)
                                                                                                                             cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                   <  1:652506/148‑1 (MQ=255)
                                                                                                                                cGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcgc                >  1:3212753/1‑148 (MQ=255)
                                                                                                                                   ttCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             >  1:346452/1‑148 (MQ=255)
                                                                                                                                   ttCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             >  2:1230520/1‑148 (MQ=255)
                                                                                                                                      ccccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCg          >  2:861028/1‑148 (MQ=255)
                                                                                                                                       cccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTTCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGc         <  2:1667111/148‑1 (MQ=255)
                                                                                                                                        ccAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCt        <  2:487994/148‑1 (MQ=255)
                                                                                                                                         cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtt       >  1:1638910/1‑148 (MQ=255)
                                                                                                                                         cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtt       <  2:1525635/148‑1 (MQ=255)
                                                                                                                                          aGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt      <  1:271165/148‑1 (MQ=255)
                                                                                                                                          aGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt      <  1:2047464/148‑1 (MQ=255)
                                                                                                                                           gcgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCACTGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtttt     >  2:2439841/1‑148 (MQ=255)
                                                                                                                                           gcgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtttt     <  2:1377164/148‑1 (MQ=255)
                                                                                                                                           gcgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtttt     <  2:1377627/148‑1 (MQ=255)
                                                                                                                                            cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTg    <  2:3356404/148‑1 (MQ=255)
                                                                                                                                              cATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGc  <  2:3096512/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGC  >  CP009273/2567066‑2567355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑