BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,579,561	G→T	100%	G158C (GGC→TGC)	narQ →	sensory histidine kinase in two‑component regulatory system with NarP (NarL)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,579,561	0	G	T	98.9%	606.1 / ‑1.7	186	G158C (GGC→TGC)	narQ	sensory histidine kinase in two‑component regulatory system with NarP (NarL)
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (103/81); total (103/83)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGC  >  CP009273/2579416‑2579702

  ataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCAt                                                                                                                                           <  1:3397424/148‑1 (MQ=255)

     atCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCgg                                                                                                                                        >  1:2047180/1‑148 (MQ=255)

       cGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTa                                                                                                                                      >  2:201426/1‑148 (MQ=255)

         gCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAtt                                                                                                                                    <  2:425311/148‑1 (MQ=255)

         gCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAtt                                                                                                                                    <  2:1121407/148‑1 (MQ=255)

          cTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAttt                                                                                                                                   <  2:2498767/148‑1 (MQ=255)

          cTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAttt                                                                                                                                   <  2:2777566/148‑1 (MQ=255)

          cTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAttt                                                                                                                                   >  1:3434870/1‑148 (MQ=255)

          cTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAttt                                                                                                                                   >  1:3434737/1‑148 (MQ=255)

           tCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAtttt                                                                                                                                  <  1:1164376/148‑1 (MQ=255)

           tCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAtttt                                                                                                                                  <  1:3359735/148‑1 (MQ=255)

            cAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTc                                                                                                                                 <  2:3308791/148‑1 (MQ=255)

            cAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTc                                                                                                                                 <  2:365175/148‑1 (MQ=255)

                  gggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTg                                                                                                                           >  2:2197524/1‑148 (MQ=255)

                   ggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTgg                                                                                                                          >  1:2987203/1‑148 (MQ=255)

                   ggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTgg                                                                                                                          >  1:648408/1‑148 (MQ=255)

                    gCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGt                                                                                                                         <  2:3107153/148‑1 (MQ=255)

                     cGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTc                                                                                                                        <  2:2964598/148‑1 (MQ=255)

                        tttGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCttt                                                                                                                     <  2:723935/148‑1 (MQ=255)

                         ttGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCtttt                                                                                                                    <  2:1238354/148‑1 (MQ=255)

                              gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACt                                                                                                               <  1:3451086/148‑1 (MQ=255)

                               tGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACtt                                                                                                              <  2:52015/148‑1 (MQ=255)

                                 gTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTg                                                                                                            >  1:2518781/1‑148 (MQ=255)

                                   aTCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTgcg                                                                                                          <  1:2623003/148‑1 (MQ=255)

                                   aTCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTgcg                                                                                                          >  1:1934288/1‑148 (MQ=255)

                                    tCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTgcgc                                                                                                         >  1:723411/1‑148 (MQ=255)

                                        gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCa                                                                                                     <  2:2447229/148‑1 (MQ=255)

                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGACGCAt                                                                                                    >  2:2307399/1‑148 (MQ=255)

                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTAACGCTGGTCTTTTTTAATTTGAGACGCAt                                                                                                    >  2:2705822/1‑148 (MQ=255)

                                             tatTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGc                                                                                                <  1:441117/148‑1 (MQ=255)

                                              atTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGcc                                                                                               >  1:2232927/1‑148 (MQ=255)

                                              atTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCACATACGcc                                                                                               >  1:1697252/1‑148 (MQ=255)

                                               ttAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCa                                                                                              >  2:2365114/1‑148 (MQ=255)

                                               ttAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCa                                                                                              >  1:1582528/1‑148 (MQ=255)

                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                             >  2:2594949/1‑148 (MQ=255)

                                                 aataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATc                                                                                            >  1:2282894/1‑148 (MQ=255)

                                                  ataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCa                                                                                           <  2:662320/148‑1 (MQ=255)

                                                   taatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAg                                                                                          >  1:2685064/1‑148 (MQ=255)

                                                   taatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAg                                                                                          >  1:2684449/1‑148 (MQ=255)

                                                    aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAgg                                                                                         >  1:2792494/1‑148 (MQ=255)

                                                    aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAgg                                                                                         >  1:3167474/1‑148 (MQ=255)

                                                    aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAgg                                                                                         <  2:3100047/148‑1 (MQ=255)

                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                       >  2:3348184/1‑148 (MQ=255)

                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                       <  2:3435835/148‑1 (MQ=255)

                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                       >  2:907758/1‑148 (MQ=255)

                                                        aTGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtggt                                                                                     <  1:3248622/148‑1 (MQ=255)

                                                          gTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTg                                                                                   <  1:2131146/148‑1 (MQ=255)

                                                          gTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTg                                                                                   >  1:2796067/1‑148 (MQ=255)

                                                           ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGc                                                                                  >  1:2765445/1‑148 (MQ=255)

                                                               tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCg                                                                              <  1:857188/148‑1 (MQ=255)

                                                               tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCg                                                                              >  2:2042524/1‑148 (MQ=255)

                                                               tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCg                                                                              >  2:2042424/1‑148 (MQ=255)

                                                                  gATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTg                                                                           <  1:1285840/148‑1 (MQ=255)

                                                                  gATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTg                                                                           >  2:1575385/1‑148 (MQ=255)

                                                                   aTAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGa                                                                          >  2:137930/1‑148 (MQ=255)

                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                        >  1:2724509/1‑148 (MQ=255)

                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                        >  1:2185605/1‑148 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                 >  2:3326671/1‑148 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                 <  1:221191/148‑1 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                 <  2:2792494/148‑1 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                 <  1:182212/148‑1 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                 >  2:891025/1‑148 (MQ=255)

                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCAATGGCTGGCTGCATCGGTATTATCACGCTGGTCTTTTTTAATTTGCGCCGCATACGCAATCATGTGGTTGCCCAGAAGAATCAGCTgg                                                                 >  1:47398/1‑148 (MQ=255)

                                                                                 aCTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAcc                                                            >  2:170518/1‑148 (MQ=255)

                                                                                 aCTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAcc                                                            >  2:170387/1‑148 (MQ=255)

                                                                                 aCTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAcc                                                            >  2:459949/1‑148 (MQ=255)

                                                                                 aCTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAcc                                                            >  2:450030/1‑148 (MQ=255)

                                                                                   tGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                          <  2:878565/148‑1 (MQ=255)

                                                                                   tGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                          <  2:2635678/148‑1 (MQ=255)

                                                                                    ggCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGcc                                                         >  1:1520254/1‑148 (MQ=255)

                                                                                       tttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                      <  1:312484/148‑1 (MQ=255)

                                                                                       tttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                      <  1:927776/148‑1 (MQ=255)

                                                                                        ttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTc                                                     >  1:2775852/1‑148 (MQ=255)

                                                                                        ttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTc                                                     >  1:174669/1‑148 (MQ=255)

                                                                                          aCAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAg                                                   <  2:551509/148‑1 (MQ=255)

                                                                                          aCAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAg                                                   <  2:1142705/148‑1 (MQ=255)

                                                                                             gCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGt                                                <  1:370322/148‑1 (MQ=255)

                                                                                                cTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAtt                                             >  1:1422467/1‑148 (MQ=255)

                                                                                                cTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAtt                                             >  1:2377894/1‑148 (MQ=255)

                                                                                                  aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                           <  1:113735/148‑1 (MQ=255)

                                                                                                  aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                           <  2:1824706/148‑1 (MQ=255)

                                                                                                     cTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAaca                                        <  1:1045522/148‑1 (MQ=255)

                                                                                                              aaaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGtt                               >  1:3198515/1‑148 (MQ=255)

                                                                                                                aTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGTGCAGTTCg                             >  2:2574471/1‑148 (MQ=255)

                                                                                                                aTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCg                             >  2:2225787/1‑148 (MQ=255)

                                                                                                                aTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCg                             >  1:1993337/1‑148 (MQ=255)

                                                                                                                  gctgctGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGAc                           <  1:2591493/148‑1 (MQ=255)

                                                                                                                  gctgctGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGAc                           >  2:717266/1‑148 (MQ=255)

                                                                                                                   ctgctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACt                          >  2:3034944/1‑148 (MQ=255)

                                                                                                                   ctgctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACt                          <  2:781025/148‑1 (MQ=255)

                                                                                                                   ctgctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACt                          >  2:641148/1‑148 (MQ=255)

                                                                                                                           ggtggCGATGTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                  <  1:2765932/148‑1 (MQ=255)

                                                                                                                            gtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccgc                 <  1:2378265/148‑1 (MQ=255)

                                                                                                                             tggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCt                >  2:2373210/1‑148 (MQ=255)

                                                                                                                                cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGa             <  1:1186211/148‑1 (MQ=255)

                                                                                                                                   tttCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATAc          >  2:902364/1‑148 (MQ=255)

                                                                                                                                        ctggctggCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAcc     <  2:1363534/148‑1 (MQ=255)

                                                                                                                                         tggctggcTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCt    >  1:1824941/1‑148 (MQ=255)

                                                                                                                                         tggctggcTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCt    >  2:592910/1‑148 (MQ=255)

                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc  <  2:3255939/148‑1 (MQ=255)

GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGC  >  CP009273/2579416‑2579702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`