Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 25,546 G→T 100% D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927325,5460GT100.0% 727.2 / NA 220D114Y (GAC→TAC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (128/92);  total (128/92)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

GCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCG  >  CP009273/25403‑25689
                                                                                                                                               |                                                                                                                                               
 cTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGc                                                                                                                                            <  2:3010984/148‑1 (MQ=255)
     cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgt                                                                                                                                        <  1:1884092/148‑1 (MQ=255)
     cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgt                                                                                                                                        >  2:276610/1‑148 (MQ=255)
     cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgt                                                                                                                                        >  1:2571638/1‑148 (MQ=255)
          ttGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCAc                                                                                                                                   >  1:1548972/1‑148 (MQ=255)
           tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACg                                                                                                                                  >  2:2589229/1‑148 (MQ=255)
            ggTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACgg                                                                                                                                 >  2:3385462/1‑148 (MQ=255)
             gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGc                                                                                                                                <  2:446429/148‑1 (MQ=255)
             gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGc                                                                                                                                <  2:299786/148‑1 (MQ=255)
               tCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCtt                                                                                                                              >  2:514818/1‑148 (MQ=255)
                cTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTc                                                                                                                             <  1:3221982/148‑1 (MQ=255)
                 tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCg                                                                                                                            >  1:3460117/1‑148 (MQ=255)
                   tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGtt                                                                                                                          <  1:2910877/148‑1 (MQ=255)
                       ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCg                                                                                                                      <  1:2586382/148‑1 (MQ=255)
                         tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGat                                                                                                                    <  1:1213215/148‑1 (MQ=255)
                          aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGata                                                                                                                   >  2:2553552/1‑148 (MQ=255)
                            tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATg                                                                                                                 <  1:1104913/148‑1 (MQ=255)
                                aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCg                                                                                                             >  2:1111092/1‑148 (MQ=255)
                                aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCg                                                                                                             >  1:638319/1‑148 (MQ=255)
                                 ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                            >  2:2693586/1‑148 (MQ=255)
                                 ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                            >  1:672855/1‑148 (MQ=255)
                                 ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                            >  2:2438976/1‑148 (MQ=255)
                                  tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGAc                                                                                                           <  1:2050697/148‑1 (MQ=255)
                                  tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTGCGTTGTCGATATGATCGAc                                                                                                           >  1:3118746/1‑148 (MQ=255)
                                    gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                         >  1:3437896/1‑148 (MQ=255)
                                     tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                        >  1:2741898/1‑148 (MQ=255)
                                      aTTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATAGCTTACGCGCTGAATATTGGAGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCt                                                                                                       >  1:2943530/1‑148 (MQ=255)
                                       ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                      >  1:1255782/1‑148 (MQ=255)
                                        tAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTAc                                                                                                     >  1:2094224/1‑148 (MQ=255)
                                          gCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGt                                                                                                   >  2:1623156/1‑148 (MQ=255)
                                             tGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCgg                                                                                                >  2:1160784/1‑148 (MQ=255)
                                                 ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                            >  2:2330782/1‑148 (MQ=255)
                                                  cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                           <  2:1951040/148‑1 (MQ=255)
                                                  cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                           <  1:2410816/148‑1 (MQ=255)
                                                    ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTgg                                                                                         >  2:3022180/1‑148 (MQ=255)
                                                        gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCTCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACt                                                                                     <  1:1352360/148‑1 (MQ=255)
                                                        gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACt                                                                                     <  1:2177001/148‑1 (MQ=255)
                                                           atgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                  >  1:2372630/1‑148 (MQ=255)
                                                            tgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGc                                                                                 <  2:2028671/148‑1 (MQ=255)
                                                            tgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGc                                                                                 <  2:3481920/148‑1 (MQ=255)
                                                             gatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGcc                                                                                <  1:1915268/148‑1 (MQ=255)
                                                                 tATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCt                                                                            >  2:742311/1‑148 (MQ=255)
                                                                 tATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCt                                                                            >  2:741510/1‑148 (MQ=255)
                                                                  aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                           >  2:2681616/1‑148 (MQ=255)
                                                                  aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                           <  2:1817111/148‑1 (MQ=255)
                                                                   tCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTc                                                                          >  2:1919493/1‑148 (MQ=255)
                                                                   tCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTc                                                                          <  1:2475779/148‑1 (MQ=255)
                                                                       tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAcc                                                                      <  2:1580118/148‑1 (MQ=255)
                                                                       tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAcc                                                                      <  1:2862916/148‑1 (MQ=255)
                                                                        cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTAGCCACCTTCAAcca                                                                     >  1:2674253/1‑147 (MQ=255)
                                                                         gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                    >  2:1538376/1‑148 (MQ=255)
                                                                         gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                    >  2:1538313/1‑148 (MQ=255)
                                                                           aGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGc                                                                  >  1:1118290/1‑148 (MQ=255)
                                                                             gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCg                                                                >  2:82551/1‑148 (MQ=255)
                                                                             gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCg                                                                <  1:1394168/148‑1 (MQ=255)
                                                                             gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCg                                                                <  1:763167/148‑1 (MQ=255)
                                                                              ccACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGa                                                               <  2:3091828/148‑1 (MQ=255)
                                                                              ccACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGa                                                               <  1:2080910/148‑1 (MQ=255)
                                                                                aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                             >  2:1347937/1‑148 (MQ=255)
                                                                                aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                             >  2:1444973/1‑148 (MQ=255)
                                                                                 cGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATAc                                                            >  1:2266731/1‑148 (MQ=255)
                                                                                   cAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTg                                                          <  1:2202659/148‑1 (MQ=255)
                                                                                     gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                        >  1:1732563/1‑148 (MQ=255)
                                                                                       aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                      >  1:1493499/1‑148 (MQ=255)
                                                                                        gCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATc                                                     <  1:625714/148‑1 (MQ=255)
                                                                                          tAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                   >  2:122673/1‑148 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                >  2:296063/1‑148 (MQ=255)
                                                                                               aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg                                              <  1:929248/148‑1 (MQ=255)
                                                                                                atatCGATTACTCGCTCAATATTTGCGGCTCGCTGTGCAACCTGTTCTAACGCCTGTGGCAAGGCTTCGTTGTCGATATGATCGACTTCTACTTCTGCGACTGGCACTTCGCCACCATCAACCTTTCCGATAATGCCATCTGAATCgg                                             >  2:3254955/1‑148 (MQ=255)
                                                                                                  atCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                           >  1:3401319/1‑148 (MQ=255)
                                                                                                   tCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGc                                          >  1:56792/1‑148 (MQ=255)
                                                                                                      cTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGc                                       >  2:787124/1‑148 (MQ=255)
                                                                                                       ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                      >  1:127902/1‑148 (MQ=255)
                                                                                                       ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                      <  2:1473207/148‑1 (MQ=255)
                                                                                                            cgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAt                                 <  1:1060463/148‑1 (MQ=255)
                                                                                                            cgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAt                                 >  2:1706113/1‑148 (MQ=255)
                                                                                                               tGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                              >  1:1579008/1‑148 (MQ=255)
                                                                                                               tGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                              <  2:2450246/148‑1 (MQ=255)
                                                                                                                gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                             >  1:1664471/1‑148 (MQ=255)
                                                                                                                gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                             >  2:425939/1‑148 (MQ=255)
                                                                                                                   tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                          <  1:276629/148‑1 (MQ=255)
                                                                                                                     ttGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAGCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGa                        <  1:1311907/148‑1 (MQ=255)
                                                                                                                      tGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGaa                       >  1:482433/1‑148 (MQ=255)
                                                                                                                      tGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGaa                       <  1:2589229/148‑1 (MQ=255)
                                                                                                                        gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                     <  2:2239533/148‑1 (MQ=255)
                                                                                                                        gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                     >  2:2288934/1‑148 (MQ=255)
                                                                                                                        gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATATGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                     <  1:2553552/148‑1 (MQ=255)
                                                                                                                         cggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                    <  2:1579008/148‑1 (MQ=255)
                                                                                                                         cggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                    <  2:1255782/148‑1 (MQ=255)
                                                                                                                            cgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                 <  2:3186579/148‑1 (MQ=255)
                                                                                                                            cgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                 >  2:3219549/1‑148 (MQ=255)
                                                                                                                             gcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                <  1:3497845/148‑1 (MQ=255)
                                                                                                                               gcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTg              <  1:196683/148‑1 (MQ=255)
                                                                                                                                   ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTNGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt          <  1:1784198/148‑1 (MQ=255)
                                                                                                                                   ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt          >  1:109398/1‑148 (MQ=255)
                                                                                                                                     cAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt        >  2:595084/1‑148 (MQ=255)
                                                                                                                                     cAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACAGATTGTGCTGGAAGGTTTTTTGCCTTCt        >  2:3213013/1‑148 (MQ=255)
                                                                                                                                         cTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTagag    <  1:1456685/148‑1 (MQ=255)
                                                                                                                                           gTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCg  >  2:686034/1‑148 (MQ=255)
                                                                                                                                           gTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCg  >  1:432587/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCG  >  CP009273/25403‑25689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑