Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 100% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA99.2% 642.7 / ‑5.6 211D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base A (105/97);  minor base T (0/7);  total (105/106)
Fisher's exact test for biased strand distribution p-value = 6.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT  >  CP009273/2614023‑2614309
                                                                                                                                                 |                                                                                                                                              
ttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATAtt                                                                                                                                              >  2:3219062/1‑148 (MQ=255)
 tCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTCAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTg                                                                                                                                             >  2:1803109/1‑148 (MQ=255)
  cTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTAAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGc                                                                                                                                            >  2:78032/1‑148 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           >  1:2917419/1‑148 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           <  2:3156164/148‑1 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           <  2:2997035/148‑1 (MQ=255)
    tACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTc                                                                                                                                          >  2:363802/1‑148 (MQ=255)
     aCCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTGCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCACGCTAACCACATCGGAAGCCACTTTGCGAAACCCCATTGTGGTGTTATATTGCTCa                                                                                                                                         >  1:980368/1‑148 (MQ=255)
      ccTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCAc                                                                                                                                        >  2:851742/1‑148 (MQ=255)
        tttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                      <  1:2440603/148‑1 (MQ=255)
        tttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                      <  1:678734/148‑1 (MQ=255)
           gATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTAGCGAAAGCGCATTGTGCTGATATATTGCTCaaccaac                                                                                                                                   >  1:1658101/1‑142 (MQ=255)
            aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                  >  1:3495061/1‑148 (MQ=255)
              cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGt                                                                                                                                <  2:2696432/148‑1 (MQ=255)
                ggTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                              <  2:823647/148‑1 (MQ=255)
                  tCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCa                                                                                                                            >  2:2424745/1‑148 (MQ=255)
                     aTTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTAACGCAACAGTCCCAGCt                                                                                                                         >  1:2532795/1‑148 (MQ=255)
                          tACTGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTgtgt                                                                                                                    <  2:2106795/148‑1 (MQ=255)
                           aCCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGtt                                                                                                                   <  2:3199998/148‑1 (MQ=255)
                               ggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                 >  1:2224595/1‑146 (MQ=255)
                               ggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                 <  2:2224595/146‑1 (MQ=255)
                                   gTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGt                                                                                                           <  1:3326110/148‑1 (MQ=255)
                                   gTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGt                                                                                                           <  1:1941203/148‑1 (MQ=255)
                                    ttCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                          >  1:1098512/1‑148 (MQ=255)
                                    ttCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                          >  1:3181242/1‑148 (MQ=255)
                                     tCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTgg                                                                                                         >  1:1029695/1‑148 (MQ=255)
                                      ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTggg                                                                                                        >  1:3399340/1‑148 (MQ=255)
                                      ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATAAGTCCCAGCTTGTGTTTGACTAGTggg                                                                                                        >  1:2001231/1‑148 (MQ=255)
                                         gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgtgt                                                                                                     >  1:3455231/1‑148 (MQ=255)
                                         gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGAAGAGTGGgtgt                                                                                                     >  2:2572433/1‑148 (MQ=255)
                                          ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGtt                                                                                                    >  1:609387/1‑148 (MQ=255)
                                           cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                   >  2:3250754/1‑148 (MQ=255)
                                            ttCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                  >  1:556655/1‑148 (MQ=255)
                                            ttCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                  >  2:332673/1‑148 (MQ=255)
                                                aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                              <  1:2488609/148‑1 (MQ=255)
                                                aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                              >  1:2372392/1‑148 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                             >  2:2749371/1‑148 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                             >  1:1347922/1‑148 (MQ=255)
                                                  aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTcc                                                                                            <  2:578771/148‑1 (MQ=255)
                                                   gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCa                                                                                           <  2:2882215/148‑1 (MQ=255)
                                                   gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCa                                                                                           <  1:316491/148‑1 (MQ=255)
                                                    ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                          >  2:695545/1‑148 (MQ=255)
                                                    ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                          >  2:1839738/1‑148 (MQ=255)
                                                      aCTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGa                                                                                        <  1:710236/148‑1 (MQ=255)
                                                       cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                       <  2:3092492/148‑1 (MQ=255)
                                                             ccGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCAt                                                                                 <  1:2995586/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:405579/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:895400/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  2:68475/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:3319524/148‑1 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                        >  1:1206851/1‑148 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                        >  2:2661395/1‑148 (MQ=255)
                                                                             ggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGa                                                                 <  2:1870472/148‑1 (MQ=255)
                                                                               tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                               <  2:3296510/148‑1 (MQ=255)
                                                                                     cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGc                                                         >  1:2415100/1‑148 (MQ=255)
                                                                                       tAAGTCAGCAGGCTACCCACTTCGAAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCca                                                       >  2:1961690/1‑148 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   >  1:2648432/1‑148 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   <  1:1998578/148‑1 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   >  1:2600892/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:2078053/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:3301045/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:2509815/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              <  1:2795393/148‑1 (MQ=255)
                                                                                                 ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAAt                                            >  2:2139126/1‑148 (MQ=255)
                                                                                                 ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAAt                                            <  1:78032/148‑1 (MQ=255)
                                                                                                  gCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATc                                           >  1:1697331/1‑148 (MQ=255)
                                                                                                     aCCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGAc                                        >  1:1141073/1‑148 (MQ=255)
                                                                                                      cccACTTCGGAAGCGCGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAATCGAc                                        >  2:2938620/1‑148 (MQ=255)
                                                                                                      cccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAACAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACg                                       <  2:3278098/148‑1 (MQ=255)
                                                                                                       ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACgg                                      >  2:1048530/1‑148 (MQ=255)
                                                                                                       ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACgg                                      >  2:1368913/1‑148 (MQ=255)
                                                                                                        cACTTCGGAAGCTTGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGTTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGa                                     <  1:2029029/148‑1 (MQ=255)
                                                                                                         aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGAt                                    >  1:3074874/1‑148 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAACGCGGTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTTTTTGACTTCCACTATCTTAATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATta                                  <  2:1206851/148‑1 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAACGCGCTTGGTGATTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACTATCTTAATACTCTTTCGCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATta                                  <  1:1394721/148‑1 (MQ=255)
                                                                                                              ggAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATGGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAc                               <  2:143693/148‑1 (MQ=255)
                                                                                                              ggAAGCGAGTTCGCGAAAGCGCTGGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAc                               <  2:1440836/148‑1 (MQ=255)
                                                                                                                 aGCGAGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctc                            <  2:2008892/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAACGCGCTGGGTGCTGATATAGTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1218300/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGCTCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1073857/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:3498669/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          >  2:1335321/1‑148 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          >  2:1880935/1‑148 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1651960/148‑1 (MQ=255)
                                                                                                                     aGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCttt                        >  1:472681/1‑148 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTGATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTTGAGGAGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCCTTTTAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTttc                       <  2:453574/148‑1 (MQ=255)
                                                                                                                           cgAAAGGGTTTGGTGGTTATATATTGATCACGAATCTGTCCCAGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACTATATTCATACTCTTTCTCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCaa                  <  1:1839738/148‑1 (MQ=255)
                                                                                                                              aaGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggc               >  2:521607/1‑148 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg             >  1:1318308/1‑148 (MQ=255)
                                                                                                                                  ggttGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATAGACTGATTATACCTCCTTTCTTCAAGGCGGCa           <  2:3142873/146‑1 (MQ=255)
                                                                                                                                  gcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGATTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCa           <  2:1767501/148‑1 (MQ=255)
                                                                                                                                    ttgttgCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGAATTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAat         <  2:674919/148‑1 (MQ=255)
                                                                                                                                      ggTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAatat       <  2:523101/148‑1 (MQ=255)
                                                                                                                                        tGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc     >  1:1238539/1‑148 (MQ=255)
                                                                                                                                        ggCTGATATATTGCTCACGCATCAGTCCCTGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGCGGGGCCGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc     <  1:2424745/147‑1 (MQ=255)
                                                                                                                                          cttatatatTCCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCACCAAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  1:2179044/145‑1 (MQ=255)
                                                                                                                                          cTGATATCTTGCACCCGCATCAGTCCCAGCTTGTGTTTGCCGAGTGGGTGTTTGACGTCCACGATCTTCATCCCCTTTCGCCTTTGAGGGGCAGCCAC‑AAAACAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  2:1507404/148‑1 (MQ=255)
                                                                                                                                          cTGATATATTGCTCACGCATCCGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACTATCTTCATACTCTTTCTCATTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  1:2754977/148‑1 (MQ=255)
                                                                                                                                           ttttatTTTGCTCACGCATCAGTCCCAGGTTGTTTTTGACGTGTGGTTGTTTGACTTCCACGATCTTCATACTATTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt  <  1:332673/145‑1 (MQ=255)
                                                                                                                                           tGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt  <  2:2320140/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT  >  CP009273/2614023‑2614309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑