Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,633,402 C→A 100% R178L (CGC→CTC)  hisS ← histidyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,633,4020CA98.9% 552.6 / ‑1.8 177R178L (CGC→CTC) hisShistidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (90/85);  total (91/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

CTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633259‑2633546
                                                                                                                                               |                                                                                                                                                
cTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTa                                                                                                                                              <  1:2942377/148‑1 (MQ=255)
cTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTa                                                                                                                                              <  2:1060843/148‑1 (MQ=255)
 tAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTaa                                                                                                                                             >  2:203371/1‑148 (MQ=255)
 tAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTaa                                                                                                                                             >  2:1977706/1‑148 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          <  1:3292767/148‑1 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  1:748910/1‑148 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  1:2554512/1‑148 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          <  1:2087896/148‑1 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGATGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  2:496830/1‑148 (MQ=255)
    tGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTATTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTg                                                                                                                                          >  1:1967338/1‑148 (MQ=255)
           gCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTg                                                                                                                                   <  2:722955/148‑1 (MQ=255)
            cgtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGc                                                                                                                                  <  2:1713399/148‑1 (MQ=255)
              tcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCtt                                                                                                                                >  2:1322008/1‑148 (MQ=255)
               cgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTc                                                                                                                               >  1:1627168/1‑148 (MQ=255)
                gtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTcc                                                                                                                              <  2:3195722/148‑1 (MQ=255)
                  tGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAg                                                                                                                            >  2:162770/1‑148 (MQ=255)
                    agaAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCg                                                                                                                          >  1:3205886/1‑148 (MQ=255)
                         cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCg                                                                                                                     <  2:2858434/148‑1 (MQ=255)
                           ccTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAt                                                                                                                   >  2:531866/1‑148 (MQ=255)
                            cTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATa                                                                                                                  >  2:906433/1‑148 (MQ=255)
                             tGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATag                                                                                                                 >  2:679769/1‑148 (MQ=255)
                               cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTATTTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               <  2:2334358/148‑1 (MQ=255)
                               cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               >  1:1446373/1‑148 (MQ=255)
                               cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               <  2:292708/148‑1 (MQ=255)
                                     cGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGc                                                                                                         <  1:2718913/148‑1 (MQ=255)
                                      ggATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCt                                                                                                        <  2:2372755/148‑1 (MQ=255)
                                          tttttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAag                                                                                                    <  1:403043/148‑1 (MQ=255)
                                          tttttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAGTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAag                                                                                                    >  2:251851/1‑148 (MQ=255)
                                                aaTCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAc                                                                                              >  2:2232145/1‑148 (MQ=255)
                                                  tCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCCGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGt                                                                                            >  2:934592/1‑148 (MQ=255)
                                                  tCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGCACCGATAGAGTTCAGCTCCAGAGTTACGt                                                                                            >  1:374458/1‑148 (MQ=255)
                                                   ccAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                           <  1:1053465/148‑1 (MQ=255)
                                                   ccAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                           <  1:964135/148‑1 (MQ=255)
                                                    cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGc                                                                                          <  1:2845423/148‑1 (MQ=255)
                                                     aGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCt                                                                                         <  2:3053939/148‑1 (MQ=255)
                                                      gCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTc                                                                                        <  2:181443/148‑1 (MQ=255)
                                                       cACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCAACGAGGTAATTTGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTAAAGAGTTAAGTTCTCg                                                                                       >  1:15206/1‑148 (MQ=255)
                                                          gcgcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGaa                                                                                    <  1:2753501/148‑1 (MQ=255)
                                                             cAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATa                                                                                 >  2:1851042/1‑148 (MQ=255)
                                                              aGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCCGCTCAAGAGTTACGTGCTCGGAAATAc                                                                                >  1:2275812/1‑148 (MQ=255)
                                                               gCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAcc                                                                               >  1:1684529/1‑148 (MQ=255)
                                                                cGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAccc                                                                              <  2:1584325/148‑1 (MQ=255)
                                                                 gggTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCa                                                                             <  1:835963/148‑1 (MQ=255)
                                                                  ggTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAg                                                                            >  2:2238843/1‑148 (MQ=255)
                                                                   gTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgc                                                                           >  2:3378600/1‑148 (MQ=255)
                                                                    ttAGTGTACATGCGGCGTGTGCAGTCTTCGTCCACCTTTTGGTTATTGTGCTCAAGGAAATCCACCACCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCCATAGAGTTCAGCTCAAGAGTTAAGTGCGCGGAAATAACCAgcg                                                                          >  1:1487281/1‑148 (MQ=255)
                                                                        tgtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAAAGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcg                                                                      >  1:2936958/1‑148 (MQ=255)
                                                                         gtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcgc                                                                     <  2:754519/148‑1 (MQ=255)
                                                                         gtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCACGGAAATACCCAgcgcgcgc                                                                     >  2:3212581/1‑148 (MQ=255)
                                                                          tACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGcc                                                                    >  2:2543677/1‑148 (MQ=255)
                                                                           aCATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATTCCACCAGCGCATCGAGGTAATTTGCGCGTGCTTCCAGCGAACCGATATAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGcca                                                                   >  1:997633/1‑148 (MQ=255)
                                                                              tGCGGCGTTTGCAGTCTTCGTCCAGCTTTTATTTATTCTGCTCAAGGAATTCCACCACCGCAACGAGGTAATTGGCGCGTGCTTCCAGCGAACCCCTAGAGTTCAGCACAAGAGTTACGTGCTCGGAAAAACCCACCGCGCGccacca                                                                >  1:2989284/1‑148 (MQ=255)
                                                                                 ggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCg                                                             <  2:3031577/148‑1 (MQ=255)
                                                                                   cgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCggg                                                           <  2:3075920/148‑1 (MQ=255)
                                                                                      ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        >  2:2627290/1‑148 (MQ=255)
                                                                                      ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        <  1:56458/148‑1 (MQ=255)
                                                                                         cAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGa                                                     <  1:1063721/148‑1 (MQ=255)
                                                                                          aGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAg                                                    <  1:1569830/148‑1 (MQ=255)
                                                                                            tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  >  2:2093259/1‑148 (MQ=255)
                                                                                            tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  >  2:2815819/1‑148 (MQ=255)
                                                                                              ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                <  2:1735855/148‑1 (MQ=255)
                                                                                              ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                >  2:405637/1‑148 (MQ=255)
                                                                                              ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                <  2:2267617/148‑1 (MQ=255)
                                                                                               tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                               <  1:2517759/148‑1 (MQ=255)
                                                                                                cGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAAt                                              <  2:2498923/148‑1 (MQ=255)
                                                                                                   ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                           >  1:3307389/1‑148 (MQ=255)
                                                                                                     aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGtt                                         >  2:2907220/1‑148 (MQ=255)
                                                                                                      gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                        >  2:2193776/1‑148 (MQ=255)
                                                                                                      gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                        >  1:787702/1‑148 (MQ=255)
                                                                                                      gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                        >  1:2549861/1‑148 (MQ=255)
                                                                                                          ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                    >  1:122434/1‑148 (MQ=255)
                                                                                                          ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                    <  1:1553466/148‑1 (MQ=255)
                                                                                                          ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                    >  1:3433496/1‑148 (MQ=255)
                                                                                                           tCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGt                                   <  2:1252058/148‑1 (MQ=255)
                                                                                                           tCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGt                                   <  2:3481730/148‑1 (MQ=255)
                                                                                                             tttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCg                                 <  2:2792242/148‑1 (MQ=255)
                                                                                                             tttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCg                                 <  2:3134099/148‑1 (MQ=255)
                                                                                                              ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                >  1:2561322/1‑148 (MQ=255)
                                                                                                              ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                <  1:976462/148‑1 (MQ=255)
                                                                                                              ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                                >  2:2122613/1‑148 (MQ=255)
                                                                                                                 tgctgcTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                             >  2:1072946/1‑148 (MQ=255)
                                                                                                                 tgctgcTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                             <  1:2152992/148‑1 (MQ=255)
                                                                                                                   ctgctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATcc                           >  1:2451786/1‑148 (MQ=255)
                                                                                                                      ctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGa                        >  1:186785/1‑148 (MQ=255)
                                                                                                                        cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                      <  2:2343523/148‑1 (MQ=255)
                                                                                                                        cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                      <  1:1851042/148‑1 (MQ=255)
                                                                                                                         aaGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCt                     >  2:11786/1‑148 (MQ=255)
                                                                                                                         aaGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCt                     >  2:3343908/1‑148 (MQ=255)
                                                                                                                         aaGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCt                     >  2:2744398/1‑148 (MQ=255)
                                                                                                                         aaGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCt                     <  2:2728364/148‑1 (MQ=255)
                                                                                                                           ggAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                   <  1:3098965/148‑1 (MQ=255)
                                                                                                                           ggAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                   >  1:314482/1‑148 (MQ=255)
                                                                                                                            gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                  <  1:411421/148‑1 (MQ=255)
                                                                                                                               tGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGa               >  1:3053814/1‑148 (MQ=255)
                                                                                                                                 ccaccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAcc             >  1:619837/1‑148 (MQ=255)
                                                                                                                                    ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa          >  1:3321182/1‑148 (MQ=255)
                                                                                                                                     caGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaa         <  1:1322008/148‑1 (MQ=255)
                                                                                                                                          cATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTc    >  1:2577466/1‑148 (MQ=255)
                                                                                                                                           aTCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCg   >  1:3467846/1‑148 (MQ=255)
                                                                                                                                            tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  <  2:3175139/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633259‑2633546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑