Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,651,665 G→T 100% G210G (GGC→GGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,651,6650GT100.0% 692.8 / ‑6.7 222G210G (GGC→GGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (131/90);  total (131/91)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

TACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCA  >  CP009273/2651520‑2651809
                                                                                                                                                 |                                                                                                                                                
tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                                >  1:352798/1‑148 (MQ=255)
     tCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGa                                                                                                                                           >  2:1655100/1‑148 (MQ=255)
     tCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGa                                                                                                                                           >  1:3133162/1‑148 (MQ=255)
      cAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAg                                                                                                                                          >  2:1921746/1‑148 (MQ=255)
          aaTGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCa                                                                                                                                      <  2:441733/148‑1 (MQ=255)
             gCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaa                                                                                                                                   >  1:1815773/1‑148 (MQ=255)
              cccGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaaa                                                                                                                                  <  1:734309/148‑1 (MQ=255)
                   cTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCg                                                                                                                             >  1:544959/1‑148 (MQ=255)
                     gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                           >  2:460025/1‑148 (MQ=255)
                       tCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGGTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                         <  2:264085/148‑1 (MQ=255)
                          cgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGc                                                                                                                      >  1:3045561/1‑148 (MQ=255)
                           gAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGcc                                                                                                                     <  2:2423844/148‑1 (MQ=255)
                             aTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCtt                                                                                                                   >  1:626862/1‑148 (MQ=255)
                              tttAATCCGCCAGATTATGGTCTAAATCATCGCCGCGGAGCTGGTAATCACCGCGGGTTGCAAGCACTTAAAACACGCCGCGAGTTAAGCGCTAATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                  <  1:2191913/146‑1 (MQ=255)
                              tGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                  >  2:2217220/1‑148 (MQ=255)
                              tGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                  >  2:1311966/1‑148 (MQ=255)
                               ttAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGTGCGCGTAATCACCGCCGGTTGCCACCACTTCAAACCCGCCGCGACTTAAGCGAAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTcc                                                                                                                 <  1:2556293/147‑1 (MQ=255)
                               gTAATCCGCCAGCTGTTGGTCGAAATCATCGCCGCCGAGCGCGGATACACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTcc                                                                                                                 <  1:1643233/148‑1 (MQ=255)
                                 aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACCCCCCGCGACTTAAGCGCAGAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTg                                                                                                               <  1:1965331/148‑1 (MQ=255)
                                  aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGACTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGa                                                                                                              >  2:3051965/1‑148 (MQ=255)
                                  aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGa                                                                                                              >  1:435572/1‑148 (MQ=255)
                                   tCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             >  1:3112839/1‑148 (MQ=255)
                                          gcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATc                                                                                                      >  2:2506568/1‑148 (MQ=255)
                                              aTGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAAAGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                                  >  1:2795661/1‑148 (MQ=255)
                                                ggTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGcac                                                                                                <  2:2558648/148‑3 (MQ=255)
                                                 gTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCg                                                                                               <  1:2347707/148‑1 (MQ=255)
                                                   ctaaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCTACTTATGCGCAGAATGGAAATTTCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTa                                                                                             <  1:1475223/146‑1 (MQ=255)
                                                     aaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAgg                                                                                           >  2:2993013/1‑148 (MQ=255)
                                                       atcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                         >  2:253465/1‑148 (MQ=255)
                                                        tcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGGCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGa                                                                                        <  1:1836844/148‑1 (MQ=255)
                                                         catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                       <  1:258308/148‑1 (MQ=255)
                                                          atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATa                                                                                      >  1:1173529/1‑148 (MQ=255)
                                                           tcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAg                                                                                     <  1:1921189/148‑1 (MQ=255)
                                                            cgccgcCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                    <  2:2020728/148‑1 (MQ=255)
                                                            cgccgcCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                    >  1:791206/1‑148 (MQ=255)
                                                                   gAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcg                                                                             <  2:938818/148‑1 (MQ=255)
                                                                   gAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcg                                                                             <  2:515609/148‑1 (MQ=255)
                                                                        cgGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcgg                                                                        <  1:537932/148‑1 (MQ=255)
                                                                        cgGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcgg                                                                        >  1:2117901/1‑148 (MQ=255)
                                                                        cgGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcgg                                                                        <  2:1117089/148‑1 (MQ=255)
                                                                         ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACTTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcggt                                                                       <  2:716692/148‑1 (MQ=255)
                                                                         ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACTTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcggt                                                                       <  2:716924/148‑1 (MQ=255)
                                                                          gAATCACCGCGGGTTCCCAGCACTACAACCCCGCCGCGACTTAAGCGCAGATTGTAAATACCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGtt                                                                      <  1:346608/148‑1 (MQ=255)
                                                                            aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCg                                                                    >  2:94898/1‑148 (MQ=255)
                                                                            aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCg                                                                    >  2:94669/1‑148 (MQ=255)
                                                                            aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCg                                                                    <  1:2027698/148‑1 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                   <  1:2824514/148‑1 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                   >  1:1900301/1‑148 (MQ=255)
                                                                                 cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                               >  2:1951064/1‑148 (MQ=255)
                                                                                 cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                               >  2:2321763/1‑148 (MQ=255)
                                                                                   ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGACTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagta                                                             >  2:3280074/1‑148 (MQ=255)
                                                                                     ggTTGCCACAACTACAAACCCCCCGCGAATTAAGCGAAGAATGTAAATAACACCCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                           <  1:2943968/148‑1 (MQ=255)
                                                                                           cAGCACTTCAAACCCGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAgg                                                     <  1:719393/148‑1 (MQ=255)
                                                                                            aGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTCGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGa                                                    >  1:1866585/1‑148 (MQ=255)
                                                                                            aGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGa                                                    <  1:2463485/148‑1 (MQ=255)
                                                                                             gCACTTCAAACACGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAc                                                   >  2:200193/1‑148 (MQ=255)
                                                                                              cACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                  >  2:1576323/1‑148 (MQ=255)
                                                                                              cACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                  >  2:865821/1‑148 (MQ=255)
                                                                                                 ttCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGa                                               >  2:2208119/1‑148 (MQ=255)
                                                                                                 ttCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGa                                               <  2:269626/148‑1 (MQ=255)
                                                                                                  tCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGaa                                              <  1:2567750/148‑1 (MQ=255)
                                                                                                     aaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGc                                           <  2:626862/148‑1 (MQ=255)
                                                                                                      acacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGcc                                          >  2:561254/1‑148 (MQ=255)
                                                                                                      acacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGcc                                          >  2:2177122/1‑148 (MQ=255)
                                                                                                      acacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCACTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAATCGCAGGACGTGAAGGcc                                          >  1:2273921/1‑148 (MQ=255)
                                                                                                         cgccgcGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGc                                       >  1:1066420/1‑148 (MQ=255)
                                                                                                             gcgACTTAAGCGCAGAATGGAAATCTCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGa                                   <  1:830491/148‑1 (MQ=255)
                                                                                                             gcgACTTAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGa                                   <  1:678542/148‑1 (MQ=255)
                                                                                                                 cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               >  2:2105034/1‑148 (MQ=255)
                                                                                                                 cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               >  1:3342575/1‑148 (MQ=255)
                                                                                                                 cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               >  1:1087738/1‑148 (MQ=255)
                                                                                                                 cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCATGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               >  1:1833205/1‑148 (MQ=255)
                                                                                                                  ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                              >  2:3028053/1‑148 (MQ=255)
                                                                                                                  ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                              >  2:3027221/1‑148 (MQ=255)
                                                                                                                   tAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgcc                             >  1:2693612/1‑148 (MQ=255)
                                                                                                                     aGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgccgc                           >  1:1698095/1‑148 (MQ=255)
                                                                                                                            aaTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                    >  1:3504213/1‑148 (MQ=255)
                                                                                                                            aaTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                    >  2:3378352/1‑148 (MQ=255)
                                                                                                                             aTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTgg                   <  2:685181/148‑1 (MQ=255)
                                                                                                                               ggAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg                 >  1:3009230/1‑148 (MQ=255)
                                                                                                                               ggAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg                 >  1:1949177/1‑148 (MQ=255)
                                                                                                                               ggAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg                 >  1:1948965/1‑148 (MQ=255)
                                                                                                                                 aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc               >  1:2707139/1‑148 (MQ=255)
                                                                                                                                    tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg            >  2:2963373/1‑148 (MQ=255)
                                                                                                                                    tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg            <  2:2601/148‑1 (MQ=255)
                                                                                                                                    tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg            >  2:1413174/1‑148 (MQ=255)
                                                                                                                                     atCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa           >  1:1584950/1‑148 (MQ=255)
                                                                                                                                     atCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa           >  2:2530160/1‑148 (MQ=255)
                                                                                                                                     atCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa           <  1:1997749/148‑1 (MQ=255)
                                                                                                                                     atCAAACGTCCCTCCACCGAGGTCATAAACGGAGATCACGCCTTCCTCACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTATGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa           >  1:1586027/1‑148 (MQ=255)
                                                                                                                                      tCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGAc          >  2:3410025/1‑148 (MQ=255)
                                                                                                                                       cAACCGTCCCTCCACCGAGGTCATAACCGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACg         <  1:3433686/148‑1 (MQ=255)
                                                                                                                                         aaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCt       >  2:619219/1‑148 (MQ=255)
                                                                                                                                          aCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg      >  1:1052374/1‑148 (MQ=255)
                                                                                                                                          aCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg      >  1:3180025/1‑148 (MQ=255)
                                                                                                                                           cGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTgg     >  1:2416562/1‑148 (MQ=255)
                                                                                                                                           cGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTgg     >  2:1048075/1‑148 (MQ=255)
                                                                                                                                            gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTggg    <  1:2308410/148‑1 (MQ=255)
                                                                                                                                             tCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGc   <  1:1165416/148‑1 (MQ=255)
                                                                                                                                              cccTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGcc  >  2:3430324/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCA  >  CP009273/2651520‑2651809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑