Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,659,594 C→A 100% L267L (CTC→CTA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,659,5940CA97.9% 599.8 / 3.2 190L267L (CTC→CTA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (86/100);  total (87/103)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTT  >  CP009273/2659450‑2659735
                                                                                                                                                |                                                                                                                                             
ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                            <  2:2676224/148‑1 (MQ=255)
    cccGATCCAGTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAgg                                                                                                                                        <  1:1561413/148‑1 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      >  1:1730888/1‑148 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      <  2:1701030/148‑1 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      >  1:1366469/1‑148 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      <  1:142793/148‑1 (MQ=255)
        aTCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGtt                                                                                                                                    >  1:938662/1‑148 (MQ=255)
         tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                   >  2:3013054/1‑148 (MQ=255)
          ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaa                                                                                                                                  >  1:1959613/1‑148 (MQ=255)
           cATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaaa                                                                                                                                 <  2:150530/148‑1 (MQ=255)
            aTTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAt                                                                                                                                <  2:2834618/148‑1 (MQ=255)
             ttGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATc                                                                                                                               >  1:258167/1‑148 (MQ=255)
                cGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCa                                                                                                                            <  2:1825871/148‑1 (MQ=255)
                cGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCa                                                                                                                            <  2:1825883/148‑1 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                          <  2:2397733/148‑1 (MQ=255)
                    aTGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgttt                                                                                                                        <  2:1990617/148‑1 (MQ=255)
                      gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                      <  2:118581/148‑1 (MQ=255)
                       cgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgtt                                                                                                                     >  2:2480144/1‑148 (MQ=255)
                           tCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGcc                                                                                                                 >  2:682121/1‑148 (MQ=255)
                             aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                               <  2:1042364/148‑1 (MQ=255)
                             aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                               <  2:384729/148‑1 (MQ=255)
                              gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                              >  1:409098/1‑148 (MQ=255)
                               cGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCg                                                                                                             <  2:2786219/148‑1 (MQ=255)
                                gggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCgg                                                                                                            <  2:2842434/148‑1 (MQ=255)
                                gggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCgg                                                                                                            <  2:2843692/148‑1 (MQ=255)
                                 ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                           <  2:869571/148‑1 (MQ=255)
                                  gacagacaATTGCGTCAGTCTATCACACGTCTGGTAAACCATTGTCGTGAGCTGGGAAATGTTCGTTTGTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTa                                                                                                          <  1:3023081/148‑1 (MQ=255)
                                    cagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAtt                                                                                                        >  2:653041/1‑148 (MQ=255)
                                     agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                       <  2:1426277/148‑1 (MQ=255)
                                     agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                       <  1:2319804/148‑1 (MQ=255)
                                        caATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGata                                                                                                    <  2:2751145/148‑1 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                  <  2:80419/148‑1 (MQ=255)
                                            tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAc                                                                                                <  1:3304743/148‑1 (MQ=255)
                                              cGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAccc                                                                                              <  1:82403/148‑1 (MQ=255)
                                                tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                            <  2:3187617/148‑1 (MQ=255)
                                                tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                            >  2:1099666/1‑148 (MQ=255)
                                                tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                            <  1:43463/148‑1 (MQ=255)
                                                 cAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGc                                                                                           >  1:956960/1‑148 (MQ=255)
                                                 cAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGc                                                                                           <  1:1874633/148‑1 (MQ=255)
                                                     cTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGCTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGa                                                                                       >  1:481488/1‑148 (MQ=255)
                                                     cTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAAAAGGGGTTATGTTATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGa                                                                                       >  1:1230894/1‑148 (MQ=255)
                                                       aTCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAag                                                                                     <  2:2450774/148‑1 (MQ=255)
                                                         cAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAagag                                                                                   <  2:2199919/148‑1 (MQ=255)
                                                          aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGt                                                                                  <  1:259500/148‑1 (MQ=255)
                                                          aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGt                                                                                  >  1:1669341/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                 >  2:3428566/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                 >  1:1940358/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                 >  2:2140012/1‑148 (MQ=255)
                                                             cGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCg                                                                               <  2:3322024/148‑1 (MQ=255)
                                                                  ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                          >  2:541/1‑148 (MQ=255)
                                                                    tAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACt                                                                        <  2:2450954/148‑1 (MQ=255)
                                                                     aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTg                                                                       >  2:445336/1‑148 (MQ=255)
                                                                         cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                   >  2:2992915/1‑148 (MQ=255)
                                                                          aTTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAccc                                                                  <  1:665532/148‑1 (MQ=255)
                                                                          aTTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAccc                                                                  <  1:1264961/148‑1 (MQ=255)
                                                                           ttttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCa                                                                 <  1:211554/148‑1 (MQ=255)
                                                                              tCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGc                                                              <  2:2418899/148‑1 (MQ=255)
                                                                               cGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGcc                                                             <  1:1856793/148‑1 (MQ=255)
                                                                                  gAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTgg                                                          <  1:1054787/148‑1 (MQ=255)
                                                                                       gggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTgcgc                                                     <  2:1959613/148‑1 (MQ=255)
                                                                                         gAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcac                                                   >  1:1712571/1‑148 (MQ=255)
                                                                                          aaaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTCTTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacc                                                  >  1:2967917/1‑148 (MQ=255)
                                                                                            aTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcaccac                                                >  1:3317719/1‑148 (MQ=255)
                                                                                             tGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCaccacc                                               >  1:707729/1‑148 (MQ=255)
                                                                                              gTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCAccc                                              >  2:1446735/1‑148 (MQ=255)
                                                                                                   tttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgcgcg                                         >  2:2247528/1‑148 (MQ=255)
                                                                                                   tttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgcgcg                                         >  2:3113002/1‑148 (MQ=255)
                                                                                                    ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                        >  2:1772276/1‑148 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                      <  2:1906765/148‑1 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                      <  2:1456055/148‑1 (MQ=255)
                                                                                                          gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                  <  2:2617725/148‑1 (MQ=255)
                                                                                                           atgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGc                                 >  2:2744024/1‑148 (MQ=255)
                                                                                                             gaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCg                               <  1:3171043/148‑1 (MQ=255)
                                                                                                             gaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCg                               >  1:1535171/1‑148 (MQ=255)
                                                                                                             gaACAGGGGTTATGTGATCAGCTTTATACCCACCGAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCg                               >  1:3276116/1‑148 (MQ=255)
                                                                                                               aCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGa                             >  1:1039343/1‑148 (MQ=255)
                                                                                                               aCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGa                             >  1:437247/1‑148 (MQ=255)
                                                                                                                cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                            <  2:569476/148‑1 (MQ=255)
                                                                                                                cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                            <  2:527539/148‑1 (MQ=255)
                                                                                                                 aGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAtt                           <  1:494022/148‑1 (MQ=255)
                                                                                                                    ggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGa                        <  2:2688914/148‑1 (MQ=255)
                                                                                                                     gTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaa                       <  2:884112/148‑1 (MQ=255)
                                                                                                                      ttATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaaa                      <  1:2807429/148‑1 (MQ=255)
                                                                                                                      ttATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaaa                      <  2:1461144/148‑1 (MQ=255)
                                                                                                                       tATGTGATCAGCTATATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAg                     <  1:3269247/148‑1 (MQ=255)
                                                                                                                         tgtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTg                   >  1:1408064/1‑148 (MQ=255)
                                                                                                                          gtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGACATCGGTATTGATAATACCCTGCCGGAAGAGTTCTCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGa                  >  1:2294480/1‑148 (MQ=255)
                                                                                                                            gATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAt                <  1:1378691/148‑1 (MQ=255)
                                                                                                                             aTCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATa               >  1:3504995/1‑148 (MQ=255)
                                                                                                                              tCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAc              <  1:681970/148‑1 (MQ=255)
                                                                                                                               cAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACg             >  1:2654137/1‑148 (MQ=255)
                                                                                                                                 gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc           >  1:3144695/1‑148 (MQ=255)
                                                                                                                                  cTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCg          >  1:3485391/1‑148 (MQ=255)
                                                                                                                                    ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc        <  1:2096086/148‑1 (MQ=255)
                                                                                                                                    ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc        >  2:3292317/1‑148 (MQ=255)
                                                                                                                                         cccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCt   <  1:1695541/148‑1 (MQ=255)
                                                                                                                                         cccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCt   >  2:2078808/1‑148 (MQ=255)
                                                                                                                                          ccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCtt  <  1:1924988/148‑1 (MQ=255)
                                                                                                                                          ccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCtt  <  2:2919011/148‑1 (MQ=255)
                                                                                                                                          ccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCtt  >  1:1004266/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTT  >  CP009273/2659450‑2659735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑