Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 231,378 C→A 100% R26S (CGC→AGC)  yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273231,3780CA98.7% 774.2 / 0.4 242R26S (CGC→AGC) yafSputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (132/106);  total (134/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 243 total aligned reads displayed.

ATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATG  >  CP009273/231234‑231521
                                                                                                                                                |                                                                                                                                               
aTTGTCATCAAAGGCGGTAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              <  2:1352971/148‑1 (MQ=255)
aTTGTCATCAAAGGCGGGAATACTGTTAAGATTCATTGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              <  1:1010426/148‑1 (MQ=255)
aTTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCa                                                                                                                                              >  1:2465846/1‑148 (MQ=255)
   gTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAgg                                                                                                                                           <  2:421631/148‑1 (MQ=255)
     catcaAAGGCGGGAATACTGATAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                         <  2:2040498/148‑1 (MQ=255)
        caAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTg                                                                                                                                      >  2:2146110/1‑148 (MQ=255)
         aaaGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTgg                                                                                                                                     <  1:171854/148‑1 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGACAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  2:2778466/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:1630337/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:2359108/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  2:841208/1‑148 (MQ=255)
            ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                  >  1:1630386/1‑148 (MQ=255)
             gCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgcg                                                                                                                                 >  1:3022181/1‑148 (MQ=255)
                  aaTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGc                                                                                                                            <  1:2008735/148‑1 (MQ=255)
                   aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCt                                                                                                                           >  2:2884072/1‑148 (MQ=255)
                   aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCt                                                                                                                           <  2:2304172/148‑1 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  1:1400154/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  2:1764891/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  2:2641732/1‑148 (MQ=255)
                     aCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCa                                                                                                                         >  1:473444/1‑148 (MQ=255)
                      cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCaa                                                                                                                        >  1:2664088/1‑148 (MQ=255)
                       tGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAg                                                                                                                             <  2:2106067/142‑1 (MQ=255)
                        gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAcc                                                                                                                      <  1:440094/148‑1 (MQ=255)
                        gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAcc                                                                                                                      <  2:1544153/148‑1 (MQ=255)
                            aGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTg                                                                                                                  >  2:2739786/1‑148 (MQ=255)
                             gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                 <  1:2454045/148‑1 (MQ=255)
                             gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                 <  1:1093180/148‑1 (MQ=255)
                                  aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCAc                                                                                                            <  2:2828285/148‑1 (MQ=255)
                                     gATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaa                                                                                                         >  2:2199818/1‑148 (MQ=255)
                                      aTTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTACCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                        <  2:1408532/148‑1 (MQ=255)
                                        tACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAt                                                                                                      >  2:2155915/1‑148 (MQ=255)
                                         aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATg                                                                                                     >  2:3511359/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    >  2:291948/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    >  2:3367376/1‑148 (MQ=255)
                                          ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                    <  1:2474369/148‑1 (MQ=255)
                                             ctcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGGGGTTCACTAAAATGTATg                                                                                                 >  2:789065/1‑148 (MQ=255)
                                               cAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGt                                                                                               <  1:2614500/148‑1 (MQ=255)
                                                 gtgtGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTTGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGttt                                                                                             >  1:314704/1‑148 (MQ=255)
                                                  tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGtttt                                                                                            <  2:1748928/148‑1 (MQ=255)
                                                     gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt                                                                                         <  2:49220/148‑1 (MQ=255)
                                                     gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCATGATTGCTGGGGCGATTTGCCCTGGGGAAACCTTTATAGAAAGGAGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCaa                                                                                         >  1:2066445/1‑147 (MQ=255)
                                                      aaaCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATc                                                                                        <  1:1493444/148‑1 (MQ=255)
                                                        aCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTg                                                                                      <  2:1120781/148‑1 (MQ=255)
                                                         cGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGc                                                                                     <  1:2150427/148‑1 (MQ=255)
                                                          ggAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCt                                                                                    <  1:891859/148‑1 (MQ=255)
                                                              ggTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg                                                                                >  1:3368806/1‑148 (MQ=255)
                                                              ggTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg                                                                                >  2:3269320/1‑148 (MQ=255)
                                                                 tgtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt                                                                             >  2:3293194/1‑148 (MQ=255)
                                                                  gtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTg                                                                            <  2:1446896/148‑1 (MQ=255)
                                                                   tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTATGATTgg                                                                           >  1:625729/1‑148 (MQ=255)
                                                                   tgatgaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTgg                                                                           <  1:2739786/148‑1 (MQ=255)
                                                                        aaaCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCACtt                                                                      >  2:1935420/1‑148 (MQ=255)
                                                                        aaaCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAtt                                                                      >  2:2553622/1‑148 (MQ=255)
                                                                           ccGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTaa                                                                   <  1:2885123/148‑1 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:2584817/1‑148 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:239902/1‑148 (MQ=255)
                                                                                     tCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAt                                                         >  2:14624/1‑148 (MQ=255)
                                                                                       ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCa                                                       <  2:1901048/148‑1 (MQ=255)
                                                                                       ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCa                                                       <  1:3456728/148‑1 (MQ=255)
                                                                                        cTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCaa                                                      >  2:3102444/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:2810845/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  1:1255394/148‑1 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:3274955/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     >  1:3090983/1‑148 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  2:2358146/148‑1 (MQ=255)
                                                                                         tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                     <  1:304846/148‑1 (MQ=255)
                                                                                          cAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAtt                                                    >  1:2050343/1‑148 (MQ=255)
                                                                                          cAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAtt                                                    >  1:2871812/1‑148 (MQ=255)
                                                                                           aaaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTg                                                   >  1:1943817/1‑148 (MQ=255)
                                                                                            aaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGc                                                  >  1:704310/1‑148 (MQ=255)
                                                                                             aCTGTCGTGGCTCCTGATTGCTGGTGCGATTTGCCCTTGGGAAATCTTTATATCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCg                                                 <  2:2135876/148‑1 (MQ=255)
                                                                                               tGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGaa                                               <  1:3238698/148‑1 (MQ=255)
                                                                                               tGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGaa                                               <  1:1305179/148‑1 (MQ=255)
                                                                                                gTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAg                                              >  2:2346651/1‑148 (MQ=255)
                                                                                                     ggCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgc                                         >  1:3324694/1‑148 (MQ=255)
                                                                                                      gCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                        >  2:46641/1‑148 (MQ=255)
                                                                                                      gCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTgcg                                        >  2:2468398/1‑148 (MQ=255)
                                                                                                         ccTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                     <  2:704256/148‑1 (MQ=255)
                                                                                                         ccTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                     <  2:2718092/148‑1 (MQ=255)
                                                                                                           tGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGtt                                   >  2:2801307/1‑148 (MQ=255)
                                                                                                              ttGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtct                                >  1:1039550/1‑148 (MQ=255)
                                                                                                                    gggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCaa                          >  1:2285679/1‑148 (MQ=255)
                                                                                                                     ggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAg                         >  2:682860/1‑148 (MQ=255)
                                                                                                                      gCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGt                        <  1:2155915/148‑1 (MQ=255)
                                                                                                                      gCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGt                        >  1:2590717/1‑148 (MQ=255)
                                                                                                                       cGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTg                       >  2:1593672/1‑148 (MQ=255)
                                                                                                                         aTTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGaa                     >  2:1730763/1‑148 (MQ=255)
                                                                                                                         aTTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGaa                     <  2:379839/148‑1 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   >  2:1432238/1‑148 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   >  1:197685/1‑148 (MQ=255)
                                                                                                                           ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                   <  1:3447045/148‑1 (MQ=255)
                                                                                                                            tGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAGCCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt                  <  2:3188332/148‑1 (MQ=255)
                                                                                                                            tGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGt                  <  1:3105314/148‑1 (MQ=255)
                                                                                                                              cccTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAGGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGttt                <  2:1958411/148‑1 (MQ=255)
                                                                                                                                 tGGGGAAAGCTCTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTg             >  2:2421/1‑148 (MQ=255)
                                                                                                                                  ggggAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgc            >  2:2564814/1‑148 (MQ=255)
                                                                                                                                     gAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa         >  2:3052557/1‑148 (MQ=255)
                                                                                                                                          cTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGaa    <  1:1837403/148‑1 (MQ=255)
                                                                                                                                          cTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGaa    <  2:2839308/148‑1 (MQ=255)
                                                                                                                                            ttATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATg  <  1:1876154/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATG  >  CP009273/231234‑231521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑