Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,692,793 C→A 100% H226N (CAT→AAT)  yfhH → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,692,7930CA97.4% 660.7 / 6.3 217H226N (CAT→AAT) yfhHputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (109/100);  total (115/102)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

GTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATA  >  CP009273/2692648‑2692937
                                                                                                                                                 |                                                                                                                                                
gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAAt                                                                                                                                                <  1:2295176/148‑1 (MQ=255)
 tcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAAtt                                                                                                                                               <  1:2361234/148‑1 (MQ=255)
    ccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTCCCGGCTTTACTCCGACTGCCCTGCAACAGCGTGCTTCTAATTGcc                                                                                                                                            >  2:2465103/1‑148 (MQ=255)
     cTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCt                                                                                                                                           >  2:2797775/1‑148 (MQ=255)
         cGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGACAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGtct                                                                                                                                       >  2:3269963/1‑146 (MQ=255)
         cGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGtat                                                                                                                                       <  1:765874/148‑1 (MQ=255)
         cGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGtat                                                                                                                                       >  1:2348756/1‑148 (MQ=255)
            tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATAcc                                                                                                                                    >  1:190303/1‑148 (MQ=255)
              tGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAt                                                                                                                                  <  2:554526/148‑1 (MQ=255)
                 tattaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTATACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGc                                                                                                                               >  1:1631394/1‑148 (MQ=255)
                  attaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGcc                                                                                                                              >  2:335874/1‑148 (MQ=255)
                  attaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGcc                                                                                                                              >  2:2101297/1‑148 (MQ=255)
                     aGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaa                                                                                                                           <  1:107053/148‑1 (MQ=255)
                      gCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaag                                                                                                                          >  1:2525115/1‑148 (MQ=255)
                       ccATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaaga                                                                                                                         >  2:797913/1‑148 (MQ=255)
                        cATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaagaa                                                                                                                        <  1:126095/148‑1 (MQ=255)
                        cATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaagaa                                                                                                                        >  1:1013375/1‑148 (MQ=255)
                          tttCCTCCACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGACAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACa                                                                                                                      >  1:3429909/1‑148 (MQ=255)
                           ttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACaa                                                                                                                     >  1:2885481/1‑148 (MQ=255)
                               tACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGAAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGa                                                                                                                 <  1:3479970/148‑1 (MQ=255)
                                acacCGGTGTACGACGCGAGTTAAAACTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGACGAAGAACAAGCGAc                                                                                                                >  1:3062726/1‑148 (MQ=255)
                                 cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACa                                                                                                               >  1:2244826/1‑148 (MQ=255)
                                   ccGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                                                             >  2:3281372/1‑148 (MQ=255)
                                      gtgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAg                                                                                                          >  1:3502267/1‑148 (MQ=255)
                                       tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                                                         <  2:1783932/148‑1 (MQ=255)
                                       tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                                                         <  2:3488355/148‑1 (MQ=255)
                                          acgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                                                      <  2:470208/148‑1 (MQ=255)
                                          acgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                                                      <  2:1155328/148‑1 (MQ=255)
                                             acgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCa                                                                                                   >  1:2020104/1‑148 (MQ=255)
                                             acgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCa                                                                                                   >  1:2111117/1‑148 (MQ=255)
                                              cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCaa                                                                                                  <  2:88158/148‑1 (MQ=255)
                                               gcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAt                                                                                                 <  1:1439592/148‑1 (MQ=255)
                                                cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtc                                                                                                >  2:613365/1‑148 (MQ=255)
                                                cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtc                                                                                                >  1:1758732/1‑148 (MQ=255)
                                                 gAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtct                                                                                               <  1:2960292/148‑1 (MQ=255)
                                                   gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATctct                                                                                             >  2:2650305/1‑148 (MQ=255)
                                                   gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATctct                                                                                             >  2:2173030/1‑148 (MQ=255)
                                                     tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                           >  1:1320675/1‑148 (MQ=255)
                                                     tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                           <  1:1395187/148‑1 (MQ=255)
                                                     tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                           >  1:1034083/1‑148 (MQ=255)
                                                         ccTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGt                                                                                       >  2:3035480/1‑148 (MQ=255)
                                                           tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCa                                                                                     <  2:763430/148‑1 (MQ=255)
                                                           tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCa                                                                                     <  2:2525115/148‑1 (MQ=255)
                                                             gcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACg                                                                                   >  2:1498922/1‑148 (MQ=255)
                                                                   gatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAgg                                                                             >  1:2932228/1‑148 (MQ=255)
                                                                    atgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAggg                                                                            >  2:300257/1‑148 (MQ=255)
                                                                     tgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGa                                                                           <  1:2739496/148‑1 (MQ=255)
                                                                     tgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGa                                                                           >  1:2311372/1‑148 (MQ=255)
                                                                       agatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAAt                                                                         <  1:475556/148‑1 (MQ=255)
                                                                        gatgCTGCGCGTGGGCGGAATAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAAGAGCGTGCTTCTAATTGCCTGTATACGATTGCCGAAGAACATGCGACAAACAGTGCTTCTATCTCTGCTTGTCACGCTCAGGGAATg                                                                        <  2:2908563/148‑1 (MQ=255)
                                                                        gatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATg                                                                        <  2:1983015/148‑1 (MQ=255)
                                                                        gaggctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATg                                                                        <  2:1916252/145‑1 (MQ=255)
                                                                         atgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGt                                                                       <  2:2195024/148‑1 (MQ=255)
                                                                         atgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGt                                                                       >  2:2965678/1‑148 (MQ=255)
                                                                         atgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGt                                                                       >  2:54490/1‑148 (MQ=255)
                                                                           gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                     <  1:3166780/148‑1 (MQ=255)
                                                                           gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                     <  2:2348756/148‑1 (MQ=255)
                                                                           gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                     >  1:574734/1‑148 (MQ=255)
                                                                               cGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACgg                                                                 <  2:2571179/148‑1 (MQ=255)
                                                                                gAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGa                                                                <  1:817583/148‑1 (MQ=255)
                                                                                 aGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAt                                                               >  2:1672614/1‑148 (MQ=255)
                                                                                 aGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAt                                                               <  1:2570434/148‑1 (MQ=255)
                                                                                 aGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAt                                                               <  1:2569796/148‑1 (MQ=255)
                                                                                  gTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAtt                                                              <  2:1454442/148‑1 (MQ=255)
                                                                                   tGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTAACGCTCAGGGAATGTTAACGGAttt                                                             >  1:115618/1‑148 (MQ=255)
                                                                                      gCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCt                                                          <  2:849041/148‑1 (MQ=255)
                                                                                       cGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTg                                                         >  1:1427471/1‑148 (MQ=255)
                                                                                            aaGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCAt                                                    <  1:613365/148‑1 (MQ=255)
                                                                                               tGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgc                                                 >  1:2857051/1‑148 (MQ=255)
                                                                                               tGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgc                                                 >  1:2873886/1‑148 (MQ=255)
                                                                                                gCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGTTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgcg                                                <  1:3003159/148‑1 (MQ=255)
                                                                                                   ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTg                                             >  1:2885419/1‑148 (MQ=255)
                                                                                                       aTTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTc                                         >  2:2083966/1‑148 (MQ=255)
                                                                                                         tACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcag                                       >  1:10215/1‑148 (MQ=255)
                                                                                                          aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                      <  1:3281372/148‑1 (MQ=255)
                                                                                                          aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                      <  2:1070178/148‑1 (MQ=255)
                                                                                                          aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                      <  2:2873886/148‑1 (MQ=255)
                                                                                                           ccGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca                                     <  1:335874/148‑1 (MQ=255)
                                                                                                           ccGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca                                     >  1:681928/1‑148 (MQ=255)
                                                                                                              gCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGa                                  >  2:3138259/1‑148 (MQ=255)
                                                                                                               cTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGAt                                 <  1:2533596/148‑1 (MQ=255)
                                                                                                                tttACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATc                                >  1:913389/1‑148 (MQ=255)
                                                                                                                  tACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTg                              <  1:2797775/148‑1 (MQ=255)
                                                                                                                    cTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGa                            >  1:1618414/1‑148 (MQ=255)
                                                                                                                    cTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGa                            >  1:1618489/1‑148 (MQ=255)
                                                                                                                      ccGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAAc                          >  2:2845232/1‑148 (MQ=255)
                                                                                                                       cGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACt                         >  1:3335903/1‑148 (MQ=255)
                                                                                                                         aaTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTgg                       <  1:2090808/148‑1 (MQ=255)
                                                                                                                         aaTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTgg                       >  2:1931157/1‑148 (MQ=255)
                                                                                                                            gCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGACCTGGCAc                    >  2:1628044/1‑148 (MQ=255)
                                                                                                                            gCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAc                    >  1:1889975/1‑148 (MQ=255)
                                                                                                                            gCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAc                    >  2:1332282/1‑148 (MQ=255)
                                                                                                                             cccTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAcc                   >  1:702325/1‑148 (MQ=255)
                                                                                                                                 gCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGaa               >  2:1181652/1‑148 (MQ=255)
                                                                                                                                    aCAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGt            <  1:1801177/148‑1 (MQ=255)
                                                                                                                                     cAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTa           >  2:925960/1‑148 (MQ=255)
                                                                                                                                      aGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATGTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTAt          <  2:420464/148‑1 (MQ=255)
                                                                                                                                          tGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGt      >  2:3327564/1‑148 (MQ=255)
                                                                                                                                              tctaATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCTGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATa  <  2:143089/148‑1 (MQ=255)
                                                                                                                                              tctaATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATa  >  2:1587928/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATA  >  CP009273/2692648‑2692937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑