Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,880 T→A 100% N202Y (AAC→TAC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,8800TA99.2% 553.9 / ‑4.2 178N202Y (AAC→TAC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (101/75);  total (103/75)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024
                                                                                                                                                 |                                                                                                                                                
    cagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGCCGGTCGGCGTTATAGGTCTGCCCGGTACTCTTGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGaa                                                                                                                                            <  1:1100009/148‑1 (MQ=255)
     agtGCGGACAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGTGCCTTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAAc                                                                                                                                           <  1:634866/148‑1 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         >  1:2932426/1‑148 (MQ=255)
       tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCa                                                                                                                                         >  1:1891111/1‑148 (MQ=255)
          ggTCACCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:2124494/148‑1 (MQ=255)
          ggTCAACCTGTTTGGGCTTGAAGTGGCCCAGACGGTCGGCTTTATAGGTCTGCCCGGTACTCATGAATTTCACTTTGTCGCCCTTACGCTGGGTGCCGTTTTTAATCCGTATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGa                                                                                                                                      <  1:356332/148‑1 (MQ=255)
           gTCACCCTGTGTCGGCGTGAAGAGGCCCCGACGGTGGGGTTTATCGGTCTGCCCGTTACTCTTGACTTACAGTTTGTCCCCCTTACGCCGGGTGCCGTTTTTAATCGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAg                                                                                                                                     <  1:307499/148‑1 (MQ=255)
           gTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGCTAAGTGAAACAACGCCCCGGTAGTAGTCGCACCATGAg                                                                                                                                     >  1:641584/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                    >  1:1826828/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGt                                                                                                                                    >  2:2988826/1‑148 (MQ=255)
            tCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGGAGTCGAACCAAGAGt                                                                                                                                    >  1:2692006/1‑148 (MQ=255)
             cAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTc                                                                                                                                   >  1:2996116/1‑148 (MQ=255)
              aaCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCg                                                                                                                                  >  2:2969099/1‑148 (MQ=255)
               cccTGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCTTTATCGGTCTGCCCGGTACTCATGACTTACACTTTGTCGCCCTTCCGCAGGGTGCCGTTTTTAATCCGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 <  1:728114/147‑1 (MQ=255)
               aCCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGa                                                                                                                                 <  1:2239818/148‑1 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                <  2:1674488/148‑1 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:702851/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:1870830/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:3305068/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:3154931/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  1:3154929/1‑148 (MQ=255)
                ccTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGGTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCCGGTAGTAGTCGAACCATGAGTCGAt                                                                                                                                >  2:1564996/1‑148 (MQ=255)
                 cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAATTGAAACAAAGCCCAGGTAGTATTCGAACCATGatgagata                                                                                                                               >  1:3418781/1‑141 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCAGAGGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACTGATAATTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              <  1:3352144/148‑1 (MQ=255)
                  tGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATaa                                                                                                                              <  2:921504/148‑1 (MQ=255)
                    tttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAtt                                                                                                                            >  1:1179219/1‑148 (MQ=255)
                     ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTa                                                                                                                           >  1:592890/1‑148 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      <  1:2930942/148‑1 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGcc                                                                                                                      <  2:2017978/148‑1 (MQ=255)
                          cGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGcc                                                                                                                      >  1:2174638/1‑148 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:113419/148‑1 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:512450/148‑1 (MQ=255)
                            tGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTGTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTg                                                                                                                    <  2:2277172/148‑1 (MQ=255)
                              aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCa                                                                                                                  <  2:1360212/148‑1 (MQ=255)
                                gATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTTATACGGATAATTGAAACAACCCCCAGGTAGTAGTCGAACCATGATTCGATTATTATTGGCTTCAAc                                                                                                                >  1:21091/1‑148 (MQ=255)
                                gATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAAAAACCCCCAGGTAGTAGTCGAACCCTGAGTCGATAATTAGTGCCTGCAAc                                                                                                                >  1:386358/1‑148 (MQ=255)
                                 aTGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACg                                                                                                               >  1:1741501/1‑148 (MQ=255)
                                  tGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAAATAGTGCCTGCAACgg                                                                                                              >  1:2746695/1‑148 (MQ=255)
                                    cccAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACGTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGc                                                                                                            <  2:3235917/148‑1 (MQ=255)
                                       aGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCtt                                                                                                         >  2:2477253/1‑148 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTc                                                                                                        >  1:1713604/1‑148 (MQ=255)
                                        gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAATGAAACAACGCCCACGGAGTAGTAGAACCAAGAGTCGATAAATTTTTCCGGCAACCGGCCGTc                                                                                                        >  1:912745/1‑148 (MQ=255)
                                         aCGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTcc                                                                                                       >  2:2425185/1‑148 (MQ=255)
                                            gTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCAAGAGTCGATAATTATTTCCTGCAACGGGccataccga                                                                                                    >  1:2766497/1‑141 (MQ=255)
                                               ggCTTTATAGGTCGGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCTGGGGGCCTTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCg                                                                                                 <  1:321817/148‑1 (MQ=255)
                                                   ttATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCtt                                                                                             <  2:2052396/148‑1 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            >  2:2663173/1‑148 (MQ=255)
                                                    tataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTc                                                                                            >  2:3142080/1‑148 (MQ=255)
                                                     ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTcc                                                                                           <  1:2643627/148‑1 (MQ=255)
                                                     ataGGTCTGCCCGGTACTCATGACTTTCACTGTGGCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTTAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTcc                                                                                           <  1:2086292/148‑1 (MQ=255)
                                                                 ggTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATg                                                                               <  1:1400834/148‑1 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              >  1:1462696/1‑148 (MQ=255)
                                                                  gTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              <  2:333372/148‑1 (MQ=255)
                                                                  gTACTCATGACTGTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACACCGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGt                                                                              <  2:2788104/148‑1 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGCTAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                                                                            >  1:2276938/1‑148 (MQ=255)
                                                                    aCTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACCGATAAGTGAAACAACGCCCACGTAGTAGTCGAACCATGAGTCGATAATTATTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAtttac                                                                            >  1:511759/1‑144 (MQ=255)
                                                                     cTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTGTTTTATAAGGTTAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTATTTGCTTCAACGGGCCGTGCGGATCGGCCTACGGCGGCGGGATTTcgc                                                                           >  1:617642/1‑148 (MQ=255)
                                                                       cATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCGGCTCGCCTTCCGGCGGCGGAAAGTCgcgc                                                                         >  2:2759924/1‑148 (MQ=255)
                                                                         tGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCAc                                                                       >  1:3422652/1‑148 (MQ=255)
                                                                           aCTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCCCCa                                                                     >  2:3059620/1‑148 (MQ=255)
                                                                             tttCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGGCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAAAGTCGCGCACCAGa                                                                   >  1:1282502/1‑148 (MQ=255)
                                                                              ttCACTTTGTCGCCCTTACGCAGGGTGCCGTTGTTAATAAGGATAATTGAAACAAAGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACCGGCCATCCCGATCGCCTTACCGCGGCCGAATGTCCCGCAACAAAc                                                                  >  1:2060182/1‑148 (MQ=255)
                                                                                 aCTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGtt                                                               >  2:1792450/1‑148 (MQ=255)
                                                                                   tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCg                                                             <  1:328034/148‑1 (MQ=255)
                                                                                    ttGTCGCCCTTACGCTGGGTGCCTTTTTTAATACGGATAAGTGAAACCACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga                                                            <  1:32266/148‑1 (MQ=255)
                                                                                      gTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgaga                                                          <  2:1317628/148‑1 (MQ=255)
                                                                                       tCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGaa                                                         >  1:2301609/1‑148 (MQ=255)
                                                                                          cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTATTAGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCGGATCGCCATCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt                                                      >  2:2244735/1‑148 (MQ=255)
                                                                                          cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt                                                      <  1:2317273/148‑1 (MQ=255)
                                                                                           ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc                                                     <  1:1410587/148‑1 (MQ=255)
                                                                                            cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc                                                    >  1:867681/1‑148 (MQ=255)
                                                                                              tACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTg                                                  >  2:338614/1‑148 (MQ=255)
                                                                                              tACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTAGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCACACGTTCGAGAACGTACTg                                                  >  2:338538/1‑148 (MQ=255)
                                                                                               aCGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGc                                                 >  2:1090131/1‑148 (MQ=255)
                                                                                                cGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGca                                                >  2:1374499/1‑148 (MQ=255)
                                                                                                  cAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca                                              >  2:2178032/1‑148 (MQ=255)
                                                                                                  cAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca                                              >  2:410998/1‑148 (MQ=255)
                                                                                                      gTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCaa                                          <  1:1539985/148‑1 (MQ=255)
                                                                                                          cGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc                                      >  2:2262751/1‑148 (MQ=255)
                                                                                                            tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg                                    >  1:2684452/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                   >  1:2838386/1‑148 (MQ=255)
                                                                                                             ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt                                   >  1:1679019/1‑148 (MQ=255)
                                                                                                              tttAATAGGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt                                  <  1:735636/148‑1 (MQ=255)
                                                                                                               ttAATACGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGttt                                 <  1:3105006/148‑1 (MQ=255)
                                                                                                                     cGGATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTg                           <  1:1717523/148‑1 (MQ=255)
                                                                                                                       gATAAGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa                         <  1:1775235/148‑1 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCGTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                      >  1:868987/1‑148 (MQ=255)
                                                                                                                          aaGTGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAg                      >  2:3414281/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   >  2:3231306/1‑148 (MQ=255)
                                                                                                                             tGAAACAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc                   <  2:2965623/148‑1 (MQ=255)
                                                                                                                               aaaCAACGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc                 >  1:2884540/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg               >  2:1125471/1‑148 (MQ=255)
                                                                                                                                 acaacGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACcg               >  1:1206694/1‑148 (MQ=255)
                                                                                                                                     cGCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc           >  1:619034/1‑148 (MQ=255)
                                                                                                                                      gCCCAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCg          <  2:3418781/148‑1 (MQ=255)
                                                                                                                                        ccAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGt        <  1:6158/148‑1 (MQ=255)
                                                                                                                                         cAGGTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTg       <  2:1256402/148‑1 (MQ=255)
                                                                                                                                           ggTAGTAGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc     <  1:1870830/148‑1 (MQ=255)
                                                                                                                                             tagtagTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGt   >  1:1087742/1‑148 (MQ=255)
                                                                                                                                              agtagtCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGGTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTc  >  2:804098/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTC  >  CP009273/2699735‑2700024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑