Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,733,7530CT100.0% 572.7 / NA 174E253K (GAG→AAG) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (99/75);  total (99/75)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 182 total aligned reads displayed.

ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC  >  CP009273/2733608‑2733897
                                                                                                                                                 |                                                                                                                                                
atcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttt                                                                                                                                                <  1:824381/148‑1 (MQ=255)
  caGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttttt                                                                                                                                              <  1:1867429/148‑1 (MQ=255)
   aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttttt                                                                                                                                             <  2:1384697/148‑1 (MQ=255)
   aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttttt                                                                                                                                             <  2:1942090/148‑1 (MQ=255)
     aCCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                           >  2:3402666/1‑148 (MQ=255)
       cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTcaca                                                                                                                                         <  1:696173/148‑1 (MQ=255)
       cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTcaca                                                                                                                                         >  1:1810376/1‑148 (MQ=255)
       cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTcaca                                                                                                                                         >  2:3388703/1‑148 (MQ=255)
        aataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAt                                                                                                                                        <  2:1000398/148‑1 (MQ=255)
         ataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAtt                                                                                                                                       >  2:2946100/1‑148 (MQ=255)
          taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTg                                                                                                                                      <  2:491972/148‑1 (MQ=255)
          taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTg                                                                                                                                      >  2:564690/1‑148 (MQ=255)
             ttGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcgc                                                                                                                                   >  1:3327378/1‑148 (MQ=255)
             ttGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcgc                                                                                                                                   >  1:1091129/1‑148 (MQ=255)
              tGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCAACCGCAGGCTGACGGCGATAATCTTTATTGGAATTAACGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCCCCTGTTCCATCTTTTTTTCAAATTGCGCa                                                                                                                                  >  1:2759535/1‑148 (MQ=255)
               gAGCGATTGCCATCTTTGATTTGATCAACCACGGATTCTGCCAGCGCAGGCTGACGGCGATACTCTTTATTGTAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTTCGCaa                                                                                                                                 <  2:1556294/148‑1 (MQ=255)
                aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAAc                                                                                                                                <  1:2410737/148‑1 (MQ=255)
                aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAAc                                                                                                                                >  2:852209/1‑148 (MQ=255)
                   gATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCTAAGAACCATCAGAGAGTGGCGAAGTCCCGCATGTTCCATCTTTTTTTCACATTGCGCAACATc                                                                                                                             <  1:3448107/148‑1 (MQ=255)
                   gATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATc                                                                                                                             <  2:1945269/148‑1 (MQ=255)
                     ttGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCg                                                                                                                           <  2:464760/148‑1 (MQ=255)
                         cATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAgg                                                                                                                       >  1:1062222/1‑148 (MQ=255)
                           tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGc                                                                                                                     <  2:2460568/148‑1 (MQ=255)
                            cTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                    <  1:2033503/148‑1 (MQ=255)
                            cTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                    <  1:1297163/148‑1 (MQ=255)
                             tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                   <  1:3501813/148‑1 (MQ=255)
                             tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                   <  2:2243377/148‑1 (MQ=255)
                             tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                   <  1:1838869/148‑1 (MQ=255)
                                 atttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGt                                                                                                               <  1:1574821/148‑1 (MQ=255)
                                   ttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTc                                                                                                             >  1:1406516/1‑148 (MQ=255)
                                        cAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcgc                                                                                                        <  1:489167/148‑1 (MQ=255)
                                           ccACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                     >  2:2645361/1‑148 (MQ=255)
                                           ccACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                     >  1:1121261/1‑148 (MQ=255)
                                               ggATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTCTAGTTCGGCGCTTTacca                                                                                                 >  2:1203942/1‑148 (MQ=255)
                                               ggATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTacca                                                                                                 >  2:1204229/1‑148 (MQ=255)
                                                 aTTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccacc                                                                                               >  2:442972/1‑148 (MQ=255)
                                                 aTTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccacc                                                                                               >  2:1825557/1‑148 (MQ=255)
                                                   tCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcgc                                                                                             >  2:3228428/1‑148 (MQ=255)
                                                     tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCgcgc                                                                                           <  2:1968828/148‑1 (MQ=255)
                                                       ccACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                         >  1:1706019/1‑148 (MQ=255)
                                                        cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAgg                                                                                        >  1:1520552/1‑148 (MQ=255)
                                                        cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAgg                                                                                        >  1:1520700/1‑148 (MQ=255)
                                                         aCCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGa                                                                                       >  2:1811592/1‑148 (MQ=255)
                                                          ccGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGAt                                                                                      >  1:584757/1‑148 (MQ=255)
                                                             cAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                   <  2:2710000/148‑1 (MQ=255)
                                                                gCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                >  1:1326135/1‑148 (MQ=255)
                                                                gCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                >  1:1251101/1‑148 (MQ=255)
                                                                gCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                >  1:774658/1‑148 (MQ=255)
                                                                gCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                >  1:3069883/1‑148 (MQ=255)
                                                                 cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATgg                                                                               <  1:386599/148‑1 (MQ=255)
                                                                 cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATgg                                                                               <  1:2154917/148‑1 (MQ=255)
                                                                   gACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                             >  2:409566/1‑148 (MQ=255)
                                                                          aTAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                      >  2:2042859/1‑148 (MQ=255)
                                                                               cTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTccc                                                                 <  1:400788/148‑1 (MQ=255)
                                                                               cTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTccc                                                                 >  2:1626255/1‑148 (MQ=255)
                                                                                 ttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCt                                                               >  2:3505321/1‑148 (MQ=255)
                                                                                  tATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg                                                              <  1:958053/148‑1 (MQ=255)
                                                                                        aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTg                                                        <  2:1010045/148‑1 (MQ=255)
                                                                                          ttACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                      >  2:1904210/1‑148 (MQ=255)
                                                                                          ttACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAAATGGCCGTCCGGATTCCCCTTAGTTTGTa                                                      <  2:1029451/148‑1 (MQ=255)
                                                                                             ccGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgca                                                   <  1:3174461/148‑1 (MQ=255)
                                                                                              cGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                  >  2:1057512/1‑148 (MQ=255)
                                                                                              cGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                  >  2:810306/1‑148 (MQ=255)
                                                                                              cGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                  >  2:2569658/1‑148 (MQ=255)
                                                                                                  gCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgca                                              <  2:402253/148‑1 (MQ=255)
                                                                                                    tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgcaac                                            <  1:102722/148‑1 (MQ=255)
                                                                                                     gCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcc                                           <  2:562171/148‑1 (MQ=255)
                                                                                                      cAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                          >  2:2961775/1‑148 (MQ=255)
                                                                                                        aTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgc                                        >  1:448068/1‑148 (MQ=255)
                                                                                                        aTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgc                                        >  2:682777/1‑148 (MQ=255)
                                                                                                        aTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgc                                        >  2:681476/1‑148 (MQ=255)
                                                                                                         tCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc                                       <  2:3083658/148‑1 (MQ=255)
                                                                                                         tCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc                                       <  2:2724562/148‑1 (MQ=255)
                                                                                                          cTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccc                                      >  1:1531665/1‑148 (MQ=255)
                                                                                                            aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctg                                    >  1:2584171/1‑148 (MQ=255)
                                                                                                            aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctg                                    >  1:2583281/1‑148 (MQ=255)
                                                                                                            aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctg                                    >  1:1881815/1‑148 (MQ=255)
                                                                                                             ccATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc                                   <  2:324014/148‑1 (MQ=255)
                                                                                                             ccATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc                                   <  2:3029598/148‑1 (MQ=255)
                                                                                                                tCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcctg                                >  1:200555/1‑148 (MQ=255)
                                                                                                                 cAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCACTGCCTgg                               <  1:2177327/148‑1 (MQ=255)
                                                                                                                    agaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                            <  1:2123855/148‑1 (MQ=255)
                                                                                                                    agaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                            <  1:2123836/148‑1 (MQ=255)
                                                                                                                       cGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                         <  2:3327378/148‑1 (MQ=255)
                                                                                                                              aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaacaa                  <  2:2239499/148‑1 (MQ=255)
                                                                                                                              aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaacaa                  <  2:2239360/148‑1 (MQ=255)
                                                                                                                                tCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCTCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                <  1:1904210/148‑1 (MQ=255)
                                                                                                                                tCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                <  1:264088/148‑1 (MQ=255)
                                                                                                                                tCCCGCCTGTTCCATCTTTTTTTCAAATTGCGCAACATCCGCAGGGCAATAGTTCGGCGCTTTACCACCGCGCAGGAACACATGGCCGACCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGACAACaaaa                >  1:1472831/1‑148 (MQ=255)
                                                                                                                                 cccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAAc               >  1:1749203/1‑148 (MQ=255)
                                                                                                                                  ccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACg              <  2:2143815/148‑1 (MQ=255)
                                                                                                                                   cGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg             <  1:3389364/148‑1 (MQ=255)
                                                                                                                                   cGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg             <  2:2838807/148‑1 (MQ=255)
                                                                                                                                   cGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg             <  2:2838788/148‑1 (MQ=255)
                                                                                                                                      cTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGc          >  1:2908869/1‑148 (MQ=255)
                                                                                                                                         ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGc       <  1:3155896/148‑1 (MQ=255)
                                                                                                                                         ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGc       <  1:475773/148‑1 (MQ=255)
                                                                                                                                            cATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTgg    <  1:3336538/148‑1 (MQ=255)
                                                                                                                                             aTCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTggg   >  1:3449416/1‑148 (MQ=255)
                                                                                                                                              tcttTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc  >  2:23996/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC  >  CP009273/2733608‑2733897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑