Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,743,438 C→A 100% intergenic (+19/+36) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,743,4380CA99.4% 566.7 / ‑4.2 174intergenic (+19/+36)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (79/94);  total (80/94)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCA  >  CP009273/2743291‑2743582
                                                                                                                                                   |                                                                                                                                                
aTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTc                                                                                                                                                  >  2:1734550/1‑148 (MQ=255)
  cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGc                                                                                                                                                <  2:2310793/148‑1 (MQ=255)
  cccGGTCGCAGGCACCCGCGTGCGTATGGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGc                                                                                                                                                <  2:3450557/148‑1 (MQ=255)
    cTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAt                                                                                                                                              >  1:586370/1‑148 (MQ=255)
     tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAATAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                             >  2:2477568/1‑148 (MQ=255)
     tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                             >  1:360291/1‑148 (MQ=255)
      gTCGCTGGCACCCGCGTGCTTATTGGCGATTACTATATCGATTTTCTCGCCGTACAGGAAAATATTATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGc                                                                                                                                            <  1:2510676/148‑1 (MQ=255)
      gTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGATTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGc                                                                                                                                            >  1:2840684/1‑148 (MQ=255)
            ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGcc                                                                                                                                      >  1:1644399/1‑148 (MQ=255)
             gCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                     >  1:3317706/1‑148 (MQ=255)
              cACCCGCGTGCGTATTGGCGAGTACGATAACGATATTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                    <  2:1495419/148‑1 (MQ=255)
              cACCCGCGTGCGTATGGGCTAGTACGATATCGAAATTCTCGACGTACCGTACAATATGATTACCCAGGAAAAAGTTTTACCGGTCAAACCGCTGCGCGAGAGTGTGGCGTAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                    <  2:3237855/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTCAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  2:197172/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCTGGTAAAAGTTTTGCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  2:743038/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  1:2304451/148‑1 (MQ=255)
                     gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattat                                                                                                                             <  2:177419/148‑1 (MQ=255)
                      tgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattatt                                                                                                                            >  1:3267385/1‑148 (MQ=255)
                          tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGa                                                                                                                        >  1:798349/1‑148 (MQ=255)
                                   gTACTATATCGATATTCTCGACGTACTGGACAATATTATTAAGCAGGTAAATGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGGGGTGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGaaa                                                                                                               <  1:3004465/148‑1 (MQ=255)
                                    tACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGATAGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAt                                                                                                              <  2:1528536/148‑1 (MQ=255)
                                     aCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                             >  2:2363343/1‑148 (MQ=255)
                                        atatctatatTCTCTACGTACAGGAAAATTTTATTAAGCATTTAAAAGTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                          <  1:213316/148‑1 (MQ=255)
                                        atatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                          <  1:823586/148‑1 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAACTTACGc                                                                                                         >  2:2257012/1‑148 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAACTTAAGc                                                                                                         >  2:1608090/1‑148 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                         <  2:3372313/148‑1 (MQ=255)
                                          atcgatatTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                        <  1:2542694/148‑1 (MQ=255)
                                            cgatttTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCttt                                                                                                      <  2:681319/148‑1 (MQ=255)
                                            cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCttt                                                                                                      <  1:1401346/148‑1 (MQ=255)
                                              atatTCTCGACGTACAGGACAATATTATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                    <  1:2463014/148‑1 (MQ=255)
                                              atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                    >  2:2837457/1‑148 (MQ=255)
                                                atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                  >  2:1527755/1‑148 (MQ=255)
                                                    tcGACGTACAGTAAAATATGATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGAAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCg                                                                                              <  1:1574564/148‑1 (MQ=255)
                                                     cGACGTACAGGACAATATGATTAAGCAGGTAAATGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                             <  1:3399745/148‑1 (MQ=255)
                                                     cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTGTCCTGTGAACCCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                             <  1:3398504/148‑1 (MQ=255)
                                                         gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACa                                                                                         <  2:2459885/148‑1 (MQ=255)
                                                             aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa                                                                                     >  2:1511245/1‑148 (MQ=255)
                                                              ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                    >  2:1098151/1‑148 (MQ=255)
                                                              ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                    <  1:3193579/148‑1 (MQ=255)
                                                               gACAATATGATTAAGCAGGTAAAAGTTTTTCCGGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAcc                                                                                   <  2:1729507/148‑1 (MQ=255)
                                                                    tatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                              <  2:292460/148‑1 (MQ=255)
                                                                     atGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                             >  2:2494214/1‑148 (MQ=255)
                                                                     atGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                             >  2:2201083/1‑148 (MQ=255)
                                                                       gATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgccgc                                                                           >  2:1386625/1‑148 (MQ=255)
                                                                        aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCa                                                                          >  2:1950569/1‑148 (MQ=255)
                                                                         ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCAc                                                                         >  2:723336/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  2:2339587/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  2:2339551/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAATCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  1:2402798/1‑148 (MQ=255)
                                                                           aaGCATGTAACAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:1221131/148‑1 (MQ=255)
                                                                           aaGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:1221716/148‑1 (MQ=255)
                                                                           aaGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:3154961/148‑1 (MQ=255)
                                                                              ctggTAAAAGTTTGTCCTGTGAACCCGCTGCGCGAGAGGGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    <  1:2459901/146‑1 (MQ=255)
                                                                              cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    >  1:139630/1‑148 (MQ=255)
                                                                              cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    >  1:2075594/1‑148 (MQ=255)
                                                                               agttAAATGTTTTTCCTGTGAAACCGCTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCg                                                                   <  1:1623188/145‑1 (MQ=255)
                                                                                ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                                  >  1:1865225/1‑148 (MQ=255)
                                                                                 gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGtc                                                                 >  2:3239464/1‑147 (MQ=255)
                                                                                 gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa                                                                 >  2:1106242/1‑148 (MQ=255)
                                                                                     aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                             <  2:3293085/148‑1 (MQ=255)
                                                                                      aGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                            >  1:1042205/1‑148 (MQ=255)
                                                                                        tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAt                                                          >  2:1598538/1‑148 (MQ=255)
                                                                                        tttttCCGGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAt                                                          <  2:1300551/148‑1 (MQ=255)
                                                                                         ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAtt                                                         <  1:3061764/148‑1 (MQ=255)
                                                                                               tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                   >  2:2638593/1‑148 (MQ=255)
                                                                                                gtgAACCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                  <  2:979035/148‑1 (MQ=255)
                                                                                                  taacccGCTGCGCGAGTGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                <  1:1224522/144‑1 (MQ=255)
                                                                                                  gacaCCGCTGCGCGAGAGTGTGGCGTAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                <  1:1224583/145‑1 (MQ=255)
                                                                                                     aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAcc                                             >  2:3273869/1‑148 (MQ=255)
                                                                                                      ccGCTGCGCTAGAGTGGGGCGGTGTATATAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                            <  1:2192739/148‑1 (MQ=255)
                                                                                                      ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                            >  1:1341044/1‑148 (MQ=255)
                                                                                                       cGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                           <  2:586370/148‑1 (MQ=255)
                                                                                                         cTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         <  1:3098191/148‑1 (MQ=255)
                                                                                                         cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         <  2:1757220/148‑1 (MQ=255)
                                                                                                         cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         >  1:1129968/1‑148 (MQ=255)
                                                                                                            cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGc                                      <  1:1827981/148‑1 (MQ=255)
                                                                                                              cgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                    >  2:1116508/1‑148 (MQ=255)
                                                                                                                agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATaa                                  <  2:3317706/148‑1 (MQ=255)
                                                                                                                   gtgtGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATgt                               <  1:886147/148‑2 (MQ=255)
                                                                                                                    tgtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGcc                              >  2:1039713/1‑148 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  1:2477568/148‑1 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  2:3121325/148‑1 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  2:3121415/148‑1 (MQ=255)
                                                                                                                            aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                      <  1:2704334/148‑1 (MQ=255)
                                                                                                                            aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                      >  2:1875670/1‑148 (MQ=255)
                                                                                                                             gTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                     <  1:950670/148‑1 (MQ=255)
                                                                                                                               acagAAAACGGACCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGTATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                   <  2:516948/145‑1 (MQ=255)
                                                                                                                                ccGACAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAcc                  <  1:949857/147‑1 (MQ=255)
                                                                                                                                aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCCAGTACGTTAcc                  <  1:1343612/148‑1 (MQ=255)
                                                                                                                                aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAca                  >  2:3018597/1‑147 (MQ=255)
                                                                                                                                  gAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTg                <  1:799904/148‑1 (MQ=255)
                                                                                                                                   aaaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCGACAGTAACCATCGCCGCACGAATCGTACCACCATTCAGCGTATAACCCATCTTCATAATGCCCAGTACGTTACCTgg               >  1:1492134/1‑148 (MQ=255)
                                                                                                                                        ggCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa          <  2:2840375/148‑1 (MQ=255)
                                                                                                                                            cGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc      <  1:1033655/148‑1 (MQ=255)
                                                                                                                                            cGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc      <  1:1033490/148‑1 (MQ=255)
                                                                                                                                             ggCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca     <  1:323540/148‑1 (MQ=255)
                                                                                                                                              gCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcat    >  2:2432547/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTCTAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  2:1273573/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  1:2812170/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  2:1273667/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCA  >  CP009273/2743291‑2743582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑