Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,836,554 G→T 100% I84I (ATC→ATA)  hycH ← hydrogenase 3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,836,5540GT98.3% 567.0 / 0.9 177I84I (ATC→ATA) hycHhydrogenase 3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (90/84);  total (91/86)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698
                                                                                                                                                 |                                                                                                                                                
tcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTAt                                                                                                                                                <  1:659358/148‑1 (MQ=255)
   tcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGAc                                                                                                                                             >  2:3484400/1‑148 (MQ=255)
    cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACg                                                                                                                                            >  2:621262/1‑148 (MQ=255)
    cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACg                                                                                                                                            >  2:3473855/1‑148 (MQ=255)
    cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACg                                                                                                                                            >  2:621266/1‑148 (MQ=255)
     aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGa                                                                                                                                           <  1:1188965/148‑1 (MQ=255)
      ccATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGAATCGGCACATGGTTAATGTCTATGACGAt                                                                                                                                          <  1:2871980/148‑1 (MQ=255)
       cATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtc                                                                                                                                         >  1:1688922/1‑148 (MQ=255)
        aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtct                                                                                                                                        >  2:1408056/1‑148 (MQ=255)
         tCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtctc                                                                                                                                       <  1:496820/148‑1 (MQ=255)
          cAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCa                                                                                                                                      >  2:1735280/1‑148 (MQ=255)
            aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAcc                                                                                                                                    >  1:2602863/1‑148 (MQ=255)
               aGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCata                                                                                                                                 <  1:955345/148‑1 (MQ=255)
                gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                >  1:2053363/1‑148 (MQ=255)
                gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                >  1:2930181/1‑148 (MQ=255)
                gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                >  2:479786/1‑148 (MQ=255)
                gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTGTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                <  1:875647/148‑1 (MQ=255)
                  tGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAttt                                                                                                                              >  2:2478101/1‑148 (MQ=255)
                  tGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAttt                                                                                                                              >  1:1554455/1‑148 (MQ=255)
                       ttttCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCa                                                                                                                         >  1:2990287/1‑148 (MQ=255)
                               atgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                 >  2:580346/1‑148 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                <  1:2934296/148‑1 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                <  1:2828224/148‑1 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                <  1:2828301/148‑1 (MQ=255)
                                 gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAt                                                                                                               <  2:1270893/148‑1 (MQ=255)
                                  aaTATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAtt                                                                                                              <  2:2583133/148‑1 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  2:1213024/1‑148 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  1:3343013/1‑148 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  2:1212993/1‑148 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  2:2962244/1‑148 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  1:1919915/1‑148 (MQ=255)
                                    tatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAtttt                                                                                                            <  2:3360601/148‑1 (MQ=255)
                                    tatCATGCAGCATAATTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTATATCGAATGCGTTGGACAGCATCGGCACATGGTTAATGTCTATGACTATCTCACCATATTTCGGCACGCCTTCCAtttt                                                                                                            >  1:772769/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  2:1645663/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  1:3115283/1‑148 (MQ=255)
                                        aTGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcgg                                                                                                        <  1:2717901/148‑1 (MQ=255)
                                        aTGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcgg                                                                                                        <  1:1263787/148‑1 (MQ=255)
                                          gcagcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcggcg                                                                                                      <  2:3008580/148‑1 (MQ=255)
                                                tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                >  2:863538/1‑148 (MQ=255)
                                                tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                <  2:1371503/148‑1 (MQ=255)
                                                tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                <  1:2677677/148‑1 (MQ=255)
                                                     aaCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgc                                                                                           <  2:1739829/148‑1 (MQ=255)
                                                     aaCAGCATTGTACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgc                                                                                           <  2:2873529/148‑1 (MQ=255)
                                                          cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                                                                      >  2:2108596/1‑148 (MQ=255)
                                                           aTTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTgcctgcct                                                                                     <  2:848493/148‑1 (MQ=255)
                                                            ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    <  1:2094039/148‑1 (MQ=255)
                                                            ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    >  2:2887685/1‑148 (MQ=255)
                                                            ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    >  1:2463359/1‑148 (MQ=255)
                                                            gtGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    <  1:2058551/147‑1 (MQ=255)
                                                             tGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCTGCACTCCTTCCATTTTGCGGCGGGCTTCACTGAATGCCTcc                                                                                   >  1:1499447/1‑148 (MQ=255)
                                                             tGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                                                                   >  2:2205176/1‑148 (MQ=255)
                                                             tGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                                                                   >  2:1902082/1‑148 (MQ=255)
                                                               tACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCag                                                                                 >  1:3190317/1‑148 (MQ=255)
                                                                 cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCagag                                                                               >  2:2979404/1‑148 (MQ=255)
                                                                 cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCagag                                                                               <  1:3442901/148‑1 (MQ=255)
                                                                 cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCagag                                                                               <  1:3442857/148‑1 (MQ=255)
                                                                  tCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGt                                                                              <  2:2811085/148‑1 (MQ=255)
                                                                   ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                             >  2:3431223/1‑148 (MQ=255)
                                                                   ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                             <  1:2555805/148‑1 (MQ=255)
                                                                   ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                             >  1:1508556/1‑148 (MQ=255)
                                                                       tCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCaa                                                                         >  1:2335468/1‑148 (MQ=255)
                                                                          tgctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATcc                                                                      <  2:730059/148‑1 (MQ=255)
                                                                          tgctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATcc                                                                      <  1:3112843/148‑1 (MQ=255)
                                                                          tgctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATcc                                                                      >  2:1029876/1‑148 (MQ=255)
                                                                           gctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCa                                                                     <  1:621262/148‑1 (MQ=255)
                                                                              gctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGc                                                                  <  2:2467966/148‑1 (MQ=255)
                                                                                      gTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt                                                          <  2:2044691/148‑1 (MQ=255)
                                                                                       tttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTc                                                         <  2:1032115/148‑1 (MQ=255)
                                                                                        ttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCa                                                        >  2:7852/1‑148 (MQ=255)
                                                                                        ttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCa                                                        <  1:702766/148‑1 (MQ=255)
                                                                                        ttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCa                                                        <  2:75612/148‑1 (MQ=255)
                                                                                        ttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCa                                                        >  2:3127521/1‑148 (MQ=255)
                                                                                         tGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCAt                                                       <  1:1424416/148‑1 (MQ=255)
                                                                                                cccGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACgg                                                >  1:2708191/1‑148 (MQ=255)
                                                                                                 ccGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACggg                                               <  1:2771327/148‑1 (MQ=255)
                                                                                                   gggCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCa                                             >  2:1845433/1‑148 (MQ=255)
                                                                                                     gCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAgg                                           <  2:2267343/148‑1 (MQ=255)
                                                                                                      cTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGt                                          <  2:3183074/148‑1 (MQ=255)
                                                                                                      cTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGt                                          <  2:3183082/148‑1 (MQ=255)
                                                                                                       tttATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTg                                         >  2:939129/1‑148 (MQ=255)
                                                                                                        ttATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGa                                        <  1:861883/148‑1 (MQ=255)
                                                                                                               aTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGcgccgc                                 >  1:1119273/1‑148 (MQ=255)
                                                                                                               aTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGcgccgc                                 >  2:3505101/1‑148 (MQ=255)
                                                                                                                tGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCt                                >  2:2646295/1‑148 (MQ=255)
                                                                                                                 gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                               >  2:2044343/1‑148 (MQ=255)
                                                                                                                 gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                               >  2:1402961/1‑148 (MQ=255)
                                                                                                                 gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                               >  1:289801/1‑148 (MQ=255)
                                                                                                                        ccAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCa                        <  1:1291583/148‑1 (MQ=255)
                                                                                                                        ccAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCa                        <  1:1649744/148‑1 (MQ=255)
                                                                                                                          aGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAAt                      <  1:1026764/148‑1 (MQ=255)
                                                                                                                            cATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCg                    >  1:3343370/1‑148 (MQ=255)
                                                                                                                               cGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATa                 >  1:3090838/1‑148 (MQ=255)
                                                                                                                                   acaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAccc             <  1:863591/148‑1 (MQ=255)
                                                                                                                                   acaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAccc             <  2:237957/148‑1 (MQ=255)
                                                                                                                                       ggTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAg         >  2:1923252/1‑148 (MQ=255)
                                                                                                                                          tAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtg      >  1:233375/1‑148 (MQ=255)
                                                                                                                                          tAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtg      >  1:1465899/1‑148 (MQ=255)
                                                                                                                                          tAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTCCCAGGCAATCGATAACCCCAAggtg      <  2:1688922/148‑1 (MQ=255)
                                                                                                                                           aaTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtgg     <  2:910271/148‑1 (MQ=255)
                                                                                                                                            aTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtggt    <  1:1846624/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑