Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,841,044 C→A 100% A519A (GCG→GCT)  hycC ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,841,0440CA99.5% 716.3 / ‑4.3 215A519A (GCG→GCT) hycChydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (118/96);  total (118/97)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

TGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCA  >  CP009273/2840899‑2841188
                                                                                                                                                 |                                                                                                                                                
tGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGc                                                                                                                                                <  1:3214890/148‑1 (MQ=255)
tGCATAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGc                                                                                                                                                <  1:1175062/148‑1 (MQ=255)
 gCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                               <  2:1450586/148‑1 (MQ=255)
 gCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                               <  1:3329501/148‑1 (MQ=255)
 gCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                               <  1:3329422/148‑1 (MQ=255)
    tAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATa                                                                                                                                            <  2:2551463/148‑1 (MQ=255)
    tAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATa                                                                                                                                            <  1:2664700/148‑1 (MQ=255)
        ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatga                                                                                                                                        >  1:507350/1‑148 (MQ=255)
         tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       >  2:2772618/1‑148 (MQ=255)
         tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       >  2:2772651/1‑148 (MQ=255)
           aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAAtgatga                                                                                                                                     <  1:1048917/148‑1 (MQ=255)
            gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGaa                                                                                                                                    >  2:2164748/1‑148 (MQ=255)
            gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGaa                                                                                                                                    >  1:2224543/1‑148 (MQ=255)
             cACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAAt                                                                                                                                   <  2:2022975/148‑1 (MQ=255)
               ccGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATgg                                                                                                                                 >  1:3463019/1‑148 (MQ=255)
                cGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgc                                                                                                                                >  1:1243587/1‑148 (MQ=255)
                 ggCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgca                                                                                                                               >  2:602551/1‑148 (MQ=255)
                  gcgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcag                                                                                                                              >  1:793030/1‑148 (MQ=255)
                   cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                             >  1:370862/1‑148 (MQ=255)
                   cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                             >  1:2139902/1‑148 (MQ=255)
                   cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                             <  2:1391228/148‑1 (MQ=255)
                     cAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGcagcag                                                                                                                           >  2:1382560/1‑148 (MQ=255)
                     cAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGcagcag                                                                                                                           >  2:3255609/1‑148 (MQ=255)
                     cAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGcagcag                                                                                                                           >  1:1514976/1‑148 (MQ=255)
                      aaaCGCCTGTTTCACCGGGATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCATTCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCagcagc                                                                                                                          <  2:824191/148‑1 (MQ=255)
                      aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCagcagc                                                                                                                          <  1:2471849/148‑1 (MQ=255)
                      aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCagcagc                                                                                                                          <  2:663988/148‑1 (MQ=255)
                        aCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                        >  2:3378180/1‑148 (MQ=255)
                        aCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                        >  1:1017766/1‑148 (MQ=255)
                         cGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCggg                                                                                                                       <  1:462773/148‑1 (MQ=255)
                            cTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcag                                                                                                                    >  2:1668605/1‑148 (MQ=255)
                            cTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcag                                                                                                                    >  2:2760226/1‑148 (MQ=255)
                                    ccGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                            <  2:3215650/148‑1 (MQ=255)
                                    ccGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                            <  2:1072211/148‑1 (MQ=255)
                                    ccGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                            <  1:1677891/148‑1 (MQ=255)
                                     cGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagc                                                                                                           >  2:1732971/1‑148 (MQ=255)
                                     cGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagc                                                                                                           >  2:1732873/1‑148 (MQ=255)
                                      ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagca                                                                                                          >  2:2840241/1‑148 (MQ=255)
                                         aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAgcagca                                                                                                       <  2:1478118/148‑1 (MQ=255)
                                                aaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGat                                                                                                >  1:1112167/1‑148 (MQ=255)
                                                 aCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCACCGTGatc                                                                                               >  2:1823952/1‑148 (MQ=255)
                                                 aCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatc                                                                                               >  1:2573067/1‑148 (MQ=255)
                                                  ccGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                              <  2:655368/148‑1 (MQ=255)
                                                  ccGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                              >  1:790620/1‑148 (MQ=255)
                                                     tGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCg                                                                                           <  2:2439377/148‑1 (MQ=255)
                                                      gAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCgg                                                                                          >  1:1323223/1‑148 (MQ=255)
                                                      gAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCgg                                                                                          <  1:2975602/148‑1 (MQ=255)
                                                       aGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGt                                                                                         >  1:685518/1‑148 (MQ=255)
                                                         cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGGAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                       <  1:2163926/148‑1 (MQ=255)
                                                         cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                       <  1:210893/148‑1 (MQ=255)
                                                            tAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgaga                                                                                    >  2:332984/1‑148 (MQ=255)
                                                              aTCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGaaa                                                                                  >  2:2408221/1‑148 (MQ=255)
                                                              aTCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGaaa                                                                                  >  1:1130777/1‑148 (MQ=255)
                                                               tCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAAc                                                                                 >  2:1332420/1‑148 (MQ=255)
                                                                cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACg                                                                                <  2:565396/148‑1 (MQ=255)
                                                                cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACg                                                                                >  1:2934161/1‑148 (MQ=255)
                                                                 aCCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACgg                                                                               <  2:1898522/148‑1 (MQ=255)
                                                                   cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                             <  2:328281/148‑1 (MQ=255)
                                                                      tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGgtggtg                                                                          <  1:1626216/148‑1 (MQ=255)
                                                                      tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGgtggtg                                                                          >  2:2249614/1‑148 (MQ=255)
                                                                       gATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGgtgt                                                                         <  1:1921648/148‑1 (MQ=255)
                                                                       gATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGgtgt                                                                         <  2:1302121/148‑1 (MQ=255)
                                                                         tttttCGTGGTCTTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTa                                                                       >  1:850941/1‑148 (MQ=255)
                                                                          ttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAg                                                                      >  1:561295/1‑148 (MQ=255)
                                                                           tttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGc                                                                     >  2:2377096/1‑148 (MQ=255)
                                                                           tttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGc                                                                     >  2:615379/1‑148 (MQ=255)
                                                                            ttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCa                                                                    <  1:2453163/148‑1 (MQ=255)
                                                                              cGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAgg                                                                  <  2:385543/148‑1 (MQ=255)
                                                                                 ggTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTc                                                               <  2:2223159/148‑1 (MQ=255)
                                                                                   tCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCa                                                             >  2:3488939/1‑148 (MQ=255)
                                                                                       aaCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                         <  2:1992558/148‑1 (MQ=255)
                                                                                         ccGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCa                                                       >  1:3470512/1‑148 (MQ=255)
                                                                                             acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAgg                                                   <  2:884562/148‑1 (MQ=255)
                                                                                                cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                >  1:853913/1‑148 (MQ=255)
                                                                                                cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                >  2:412122/1‑148 (MQ=255)
                                                                                                  cAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGAGTTAGCAGGCTCCAGCGGCAGAGGTAcag                                              >  1:331194/1‑148 (MQ=255)
                                                                                                    ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagca                                            >  1:196691/1‑148 (MQ=255)
                                                                                                     gCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                           >  2:1258836/1‑148 (MQ=255)
                                                                                                     gCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                           >  1:1999063/1‑148 (MQ=255)
                                                                                                        gCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagcag                                        <  2:793030/148‑1 (MQ=255)
                                                                                                         cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCaga                                       >  2:2212558/1‑148 (MQ=255)
                                                                                                                 gAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                               >  1:2424669/1‑148 (MQ=255)
                                                                                                                  aaCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCgg                              <  2:1017766/148‑1 (MQ=255)
                                                                                                                    cgcgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                            >  2:3040505/1‑148 (MQ=255)
                                                                                                                          ggCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGcc                      >  2:2889485/1‑148 (MQ=255)
                                                                                                                           gCAAACGATCGCCTTTGCAAATAGCCATACTGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCa                     <  2:3192085/148‑1 (MQ=255)
                                                                                                                               aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgc                 >  2:1568844/1‑148 (MQ=255)
                                                                                                                                 gATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcgc               <  2:2139902/148‑1 (MQ=255)
                                                                                                                                 gATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcgc               <  2:370862/148‑1 (MQ=255)
                                                                                                                                   tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag             >  1:231736/1‑148 (MQ=255)
                                                                                                                                   tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag             >  1:2004618/1‑148 (MQ=255)
                                                                                                                                   tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag             >  1:3101829/1‑148 (MQ=255)
                                                                                                                                    cGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagc            >  1:1951458/1‑148 (MQ=255)
                                                                                                                                      ccTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCaa          >  2:454103/1‑148 (MQ=255)
                                                                                                                                       cTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac         >  2:1532644/1‑148 (MQ=255)
                                                                                                                                        tttGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAaca        >  1:621210/1‑148 (MQ=255)
                                                                                                                                           gCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGNTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCg     >  1:1781243/1‑148 (MQ=255)
                                                                                                                                            cgaaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc    >  2:1842424/3‑148 (MQ=255)
                                                                                                                                              aaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCa  >  1:2175397/1‑148 (MQ=255)
                                                                                                                                              aaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCa  >  2:3006769/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCA  >  CP009273/2840899‑2841188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑