Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,112 C→A 100% I206I (ATC→ATA)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,1120CA99.0% 638.0 / ‑2.0 200I206I (ATC→ATA) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (80/118);  total (81/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845967‑2846256
                                                                                                                                                 |                                                                                                                                                
tGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAgg                                                                                                                                                <  1:2418898/148‑1 (MQ=255)
     cAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCg                                                                                                                                           <  1:647791/148‑1 (MQ=255)
        gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACg                                                                                                                                        >  1:1984227/1‑148 (MQ=255)
          gAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGcc                                                                                                                                      <  1:1141596/148‑1 (MQ=255)
           aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCt                                                                                                                                     >  2:252817/1‑148 (MQ=255)
           aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCt                                                                                                                                     <  2:828206/148‑1 (MQ=255)
            aaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCta                                                                                                                                    >  1:1361503/1‑148 (MQ=255)
              gcgcGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtata                                                                                                                                  >  2:2189684/1‑148 (MQ=255)
                 cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               >  2:263958/1‑148 (MQ=255)
                 cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               <  1:41356/148‑1 (MQ=255)
                  gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                              <  1:2805257/148‑1 (MQ=255)
                  gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                              <  1:3072873/148‑1 (MQ=255)
                   tGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAAtttt                                                                                                                             <  2:535636/148‑1 (MQ=255)
                     aTGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTAt                                                                                                                           >  2:1869487/1‑148 (MQ=255)
                      tgtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATc                                                                                                                          >  1:2338709/1‑148 (MQ=255)
                      tgtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATc                                                                                                                          <  2:1360385/148‑1 (MQ=255)
                       gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         <  1:804940/148‑1 (MQ=255)
                       gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         >  2:1753174/1‑148 (MQ=255)
                        tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGc                                                                                                                        <  1:3465720/148‑1 (MQ=255)
                        tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGc                                                                                                                        <  1:1728724/148‑1 (MQ=255)
                         gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       >  2:2669153/1‑148 (MQ=255)
                         gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       <  2:666241/148‑1 (MQ=255)
                            gAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGc                                                                                                                    >  1:1051837/1‑148 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                <  1:2254640/148‑1 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                <  2:213672/148‑1 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                >  1:2205085/1‑148 (MQ=255)
                                 tttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAttt                                                                                                               >  1:1910996/1‑148 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:2921471/148‑1 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:73056/148‑1 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGAAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  1:2038379/148‑1 (MQ=255)
                                   tACTTCGTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:2088925/148‑1 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            >  2:1717421/1‑148 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            <  2:445350/148‑1 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            <  2:2575751/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:1706988/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  2:2552155/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:2429322/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:1479008/148‑1 (MQ=255)
                                       tcttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCg                                                                                                         >  1:2374771/1‑148 (MQ=255)
                                             gCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCt                                                                                                   <  2:639119/148‑1 (MQ=255)
                                                 gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                               <  1:2389682/148‑1 (MQ=255)
                                                 gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                               >  1:190350/1‑148 (MQ=255)
                                                  cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                              <  1:2717763/148‑1 (MQ=255)
                                                  cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                              <  2:3294913/148‑1 (MQ=255)
                                                    caTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGtt                                                                                            <  1:1520794/148‑1 (MQ=255)
                                                     aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATCATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           <  1:3337849/148‑1 (MQ=255)
                                                     aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           >  2:1986035/1‑148 (MQ=255)
                                                         cGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                       >  1:2930105/1‑148 (MQ=255)
                                                           cTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAt                                                                                     <  2:2970314/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATATGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    <  1:3145962/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    <  1:699907/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    >  2:1460217/1‑148 (MQ=255)
                                                             tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCGATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTc                                                                                   >  2:3036911/1‑148 (MQ=255)
                                                              aTCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCg                                                                                  >  1:3369900/1‑148 (MQ=255)
                                                               tCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGa                                                                                 >  2:2232656/1‑148 (MQ=255)
                                                                cccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGaa                                                                                <  1:16983/148‑1 (MQ=255)
                                                                  cGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                              >  2:23216/1‑148 (MQ=255)
                                                                  cGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                              >  1:3329393/1‑148 (MQ=255)
                                                                       ttGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                         >  2:2153994/1‑148 (MQ=255)
                                                                       ttGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                         >  2:173076/1‑148 (MQ=255)
                                                                        tGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGa                                                                        <  2:1559041/148‑1 (MQ=255)
                                                                          cgcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATc                                                                      >  2:258344/1‑148 (MQ=255)
                                                                           gcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATct                                                                     <  1:53033/148‑1 (MQ=255)
                                                                              gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTCTAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                  >  2:544149/1‑148 (MQ=255)
                                                                              gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                  >  2:544133/1‑148 (MQ=255)
                                                                               tttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctacta                                                                 >  2:284120/1‑148 (MQ=255)
                                                                                ttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCGATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCtactac                                                                >  2:3211412/1‑148 (MQ=255)
                                                                                ttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGGATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCtactac                                                                >  2:867112/1‑148 (MQ=255)
                                                                                 tGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACa                                                               <  2:1205468/148‑1 (MQ=255)
                                                                                  gCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACaa                                                              <  2:2359757/148‑1 (MQ=255)
                                                                                      gAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCg                                                          <  2:1983994/148‑1 (MQ=255)
                                                                                            ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                    <  1:777645/148‑1 (MQ=255)
                                                                                            ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                    <  1:2422399/148‑1 (MQ=255)
                                                                                              ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATg                                                  <  2:2962884/148‑1 (MQ=255)
                                                                                                 tAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTg                                               <  1:550632/148‑1 (MQ=255)
                                                                                                  aaCGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTgg                                              <  2:3343217/148‑1 (MQ=255)
                                                                                                     ggTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgc                                           <  1:850246/148‑1 (MQ=255)
                                                                                                       tATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcag                                         >  1:1723219/1‑148 (MQ=255)
                                                                                                        aTCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcagc                                        >  1:1128569/1‑148 (MQ=255)
                                                                                                         tCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcagca                                       >  2:1303238/1‑148 (MQ=255)
                                                                                                          cGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGcagcag                                      >  1:84428/1‑148 (MQ=255)
                                                                                                              gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaaa                                  <  2:3123850/148‑1 (MQ=255)
                                                                                                               cGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAAt                                 <  2:1291214/148‑1 (MQ=255)
                                                                                                                gTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATa                                <  1:2494562/148‑1 (MQ=255)
                                                                                                                  tCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                              >  1:1404533/1‑148 (MQ=255)
                                                                                                                   ccTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCg                             <  1:1795285/148‑1 (MQ=255)
                                                                                                                   ccTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCg                             >  1:1819175/1‑148 (MQ=255)
                                                                                                                      cgcgCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATATTTTTATCGCCAGCGTTTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGcc                          <  1:3002262/148‑1 (MQ=255)
                                                                                                                              gTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCaa                  >  1:1480738/1‑148 (MQ=255)
                                                                                                                               tCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAg                 <  1:2596518/148‑1 (MQ=255)
                                                                                                                                  cGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTa              >  2:932039/1‑148 (MQ=255)
                                                                                                                                       gTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGaa         <  1:3316599/148‑1 (MQ=255)
                                                                                                                                         aTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATc       >  1:3113059/1‑148 (MQ=255)
                                                                                                                                          tGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa      <  2:244593/148‑1 (MQ=255)
                                                                                                                                          tGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa      <  2:1621325/148‑1 (MQ=255)
                                                                                                                                           ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     <  2:1530042/148‑1 (MQ=255)
                                                                                                                                           ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     <  2:1530063/148‑1 (MQ=255)
                                                                                                                                            gTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGt    <  2:859752/148‑1 (MQ=255)
                                                                                                                                             ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa   <  2:3437091/148‑1 (MQ=255)
                                                                                                                                             ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa   >  1:589967/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845967‑2846256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑