Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,848,002 T→A 100% C102* (TGT→TGA)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,848,0020TA100.0% 772.8 / NA 232C102* (TGT→TGA) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (104/128);  total (104/128)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTT  >  CP009273/2847857‑2848145
                                                                                                                                                 |                                                                                                                                               
cgcAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTgaga                                                                                                                                               >  2:2207041/1‑148 (MQ=255)
 gcAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGaa                                                                                                                                              <  1:2783760/148‑1 (MQ=255)
   aGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                            <  1:2671674/148‑1 (MQ=255)
    gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAcc                                                                                                                                           >  1:883848/1‑148 (MQ=255)
    gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGAAGTTACGAAGAATTTTGAGAAAcc                                                                                                                                           >  2:841584/1‑148 (MQ=255)
     aCTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                          >  1:505037/1‑148 (MQ=255)
        cAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGc                                                                                                                                       >  1:1161606/1‑148 (MQ=255)
          aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCg                                                                                                                                     <  1:1321678/148‑1 (MQ=255)
           cGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGc                                                                                                                                    >  2:1652841/1‑148 (MQ=255)
             tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAg                                                                                                                                  <  2:1552559/148‑1 (MQ=255)
             tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAg                                                                                                                                  >  2:302684/1‑148 (MQ=255)
              gCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGc                                                                                                                                 <  2:1960174/148‑1 (MQ=255)
               cGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCt                                                                                                                                <  2:3446287/148‑1 (MQ=255)
                     attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGc                                                                                                                          <  1:3344432/148‑1 (MQ=255)
                     attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGc                                                                                                                          <  2:1599588/148‑1 (MQ=255)
                      ttaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCa                                                                                                                         >  2:1052439/1‑148 (MQ=255)
                       taCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAg                                                                                                                        >  1:3149098/1‑148 (MQ=255)
                        aCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAgg                                                                                                                       <  1:382180/148‑1 (MQ=255)
                         cgcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                      <  1:498514/148‑1 (MQ=255)
                         cgcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                      >  2:970329/1‑148 (MQ=255)
                         cgcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCCGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                      <  1:1814176/148‑1 (MQ=255)
                          gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTg                                                                                                                     >  1:3343682/1‑148 (MQ=255)
                            gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTggg                                                                                                                   <  2:1431361/148‑1 (MQ=255)
                             tcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGc                                                                                                                  >  1:1586648/1‑148 (MQ=255)
                             tcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGc                                                                                                                  >  2:1628901/1‑148 (MQ=255)
                             tcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGc                                                                                                                  >  2:2918165/1‑148 (MQ=255)
                               gcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCta                                                                                                                <  2:2054279/148‑1 (MQ=255)
                               gcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCta                                                                                                                <  1:1112535/148‑1 (MQ=255)
                                   gAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAcc                                                                                                            <  2:461932/148‑1 (MQ=255)
                                      aaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaa                                                                                                         >  1:3003869/1‑148 (MQ=255)
                                       aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaa                                                                                                        >  2:1419816/1‑148 (MQ=255)
                                       aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaa                                                                                                        >  1:3188505/1‑148 (MQ=255)
                                        aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaaa                                                                                                       <  1:1964565/148‑1 (MQ=255)
                                         gACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                      <  2:1047279/148‑1 (MQ=255)
                                         gACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                      >  1:1305962/1‑148 (MQ=255)
                                          acacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAtt                                                                                                     >  1:3420144/1‑148 (MQ=255)
                                             cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                                                                                                  <  2:263996/148‑1 (MQ=255)
                                             cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                                                                                                  >  1:3029801/1‑148 (MQ=255)
                                              ccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                                                                                                 >  1:563117/1‑148 (MQ=255)
                                                 cATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                              <  1:766877/148‑1 (MQ=255)
                                                   ttAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTa                                                                                            >  1:2317787/1‑148 (MQ=255)
                                                   ttAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTa                                                                                            <  1:399743/148‑1 (MQ=255)
                                                   ttAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTa                                                                                            <  1:399783/148‑1 (MQ=255)
                                                   ttAAATATGAAGACGAAACGGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTa                                                                                            <  2:2556616/148‑1 (MQ=255)
                                                    tAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                                                                                           >  2:1206119/1‑148 (MQ=255)
                                                              gACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                 >  2:509668/1‑148 (MQ=255)
                                                               aCGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGcc                                                                                <  2:3174370/148‑1 (MQ=255)
                                                                   aaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTg                                                                            >  1:665771/1‑148 (MQ=255)
                                                                    aCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                           >  2:1235259/1‑148 (MQ=255)
                                                                       gTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggcgg                                                                        >  1:3482957/1‑148 (MQ=255)
                                                                       gTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggcgg                                                                        <  1:2445840/148‑1 (MQ=255)
                                                                         tCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGgcggcg                                                                      >  1:1066262/1‑148 (MQ=255)
                                                                          cTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGcggcgg                                                                     >  1:1591259/1‑148 (MQ=255)
                                                                           tGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGa                                                                    <  2:2988733/148‑1 (MQ=255)
                                                                             gCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAg                                                                  >  1:2908250/1‑148 (MQ=255)
                                                                               acacGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                <  2:2566666/148‑1 (MQ=255)
                                                                                cacGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAATAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGACACTGGCGGCTGAAGGGc                                                               >  2:2085953/1‑148 (MQ=255)
                                                                                cacGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGc                                                               >  2:1800078/1‑148 (MQ=255)
                                                                                 acGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa                                                              <  1:1212567/148‑1 (MQ=255)
                                                                                   ggTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCata                                                            <  2:2893489/148‑1 (MQ=255)
                                                                                    gTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCatat                                                           <  1:1494712/148‑1 (MQ=255)
                                                                                      ccGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttt                                                         <  1:3506180/148‑1 (MQ=255)
                                                                                       cGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAtttt                                                        <  2:1276970/148‑1 (MQ=255)
                                                                                       cGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAtttt                                                        <  2:492355/148‑1 (MQ=255)
                                                                                          tACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtgg                                                     <  1:2209911/148‑1 (MQ=255)
                                                                                           aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggt                                                    >  1:1771792/1‑148 (MQ=255)
                                                                                           aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggt                                                    <  1:873032/148‑1 (MQ=255)
                                                                                             gcagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtgg                                                  <  1:1051350/148‑1 (MQ=255)
                                                                                              cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCTCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGc                                                 <  1:841584/148‑1 (MQ=255)
                                                                                              cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGc                                                 <  1:1604990/148‑1 (MQ=255)
                                                                                                   ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGTCGGAAGGGCATATTTTTGGTGGCTGTGa                                            <  2:1453488/148‑1 (MQ=255)
                                                                                                   ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGa                                            >  2:3143528/1‑148 (MQ=255)
                                                                                                          gcgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAtt                                     <  1:1206119/148‑1 (MQ=255)
                                                                                                          gcgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAtt                                     <  1:1207028/148‑1 (MQ=255)
                                                                                                            gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCg                                   <  2:902897/148‑1 (MQ=255)
                                                                                                            gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCg                                   <  2:883848/148‑1 (MQ=255)
                                                                                                            gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCg                                   <  1:1526286/148‑1 (MQ=255)
                                                                                                             cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                  >  1:1926012/1‑148 (MQ=255)
                                                                                                             cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                  <  1:1381378/148‑1 (MQ=255)
                                                                                                             cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                  <  2:3410464/148‑1 (MQ=255)
                                                                                                                  aTACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGa                             <  2:1522206/148‑1 (MQ=255)
                                                                                                                  aTACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGa                             <  1:2415270/148‑1 (MQ=255)
                                                                                                                           aTTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc                    <  1:1265020/148‑1 (MQ=255)
                                                                                                                           aTTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc                    <  2:2340583/148‑1 (MQ=255)
                                                                                                                           aTTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc                    <  2:774924/148‑1 (MQ=255)
                                                                                                                            ttGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                   <  1:794072/148‑1 (MQ=255)
                                                                                                                              gCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                 <  2:2816312/148‑1 (MQ=255)
                                                                                                                              gCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                 >  2:2614960/1‑148 (MQ=255)
                                                                                                                               cAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTgg                <  2:2151831/148‑1 (MQ=255)
                                                                                                                                 gTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg              <  2:349581/148‑1 (MQ=255)
                                                                                                                                   tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCg            >  2:3497461/1‑148 (MQ=255)
                                                                                                                                   tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCg            >  2:621488/1‑148 (MQ=255)
                                                                                                                                   tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCg            >  2:1859210/1‑148 (MQ=255)
                                                                                                                                    aCGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa           <  2:1305962/148‑1 (MQ=255)
                                                                                                                                     cGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa          >  1:3201879/1‑148 (MQ=255)
                                                                                                                                         gaaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAACTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAag      >  2:1400799/1‑148 (MQ=255)
                                                                                                                                          aaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAaga     >  1:1768270/1‑148 (MQ=255)
                                                                                                                                          aaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAaga     <  1:2109742/148‑1 (MQ=255)
                                                                                                                                            ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGt   >  1:3435283/1‑148 (MQ=255)
                                                                                                                                             tttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGtt  >  2:1910765/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTT  >  CP009273/2847857‑2848145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑